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Electron Microscopy of Plant Pathogens (Paperback, Softcover reprint of the original 1st ed. 1991)
Loot Price: R2,819
Discovery Miles 28 190
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Electron Microscopy of Plant Pathogens (Paperback, Softcover reprint of the original 1st ed. 1991)
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Total price: R2,829
Discovery Miles: 28 290
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Plants, fungi, and viruses were among the first biological objects
studied with an electron microscope. One of the two first
instruments built by Siemens was used by Helmut Ruska, a brother of
Ernst Ruska, the pioneer in constructing electron microscopes. H.
Ruska published numerous papers on different biological objects in
1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch,
and H. Ruska of tobacco mosaic virus opened a new age in
microscopy. The main problem was then as it still is today, to
obtain an appropriate preparation of the specimen for observation
in the electron microscope. Beam damage and specimen thickness were
the first obstacles to be met. L. Marton in Brussels not only built
his own instrument, but also made considerable progress in specimen
preparation by introducing the impregnation of samples with heavy
metals to obtain useful contrast. His pictures of the bird nest
orchid root impregnated with osmium were revolutionary when
published in 1934. It is not the place here to recall the different
techniques which were developed in the subsequent years to attain
the modern knowledge on the fine structure of plant cells and of
different plant pathogens. The tremendous progress obtained with
tobacco mosaic virus is reflected in the chapter by M. Wurtz on the
fine structure of viruses in this Volume. New cytochemical and
immunological techniques considerably surpass the morphological
information obtained from the pathogens, especially at the
host-parasite interface.
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