Adenovirus - Methods and Protocols (Paperback, Softcover reprint of the original 3rd ed. 2014)

Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 1089
Release date:	September 2016
First published:	2014
Editors:	Miguel Chillon • Assumpcio Bosch
Dimensions:	254 x 178 x 14mm (L x W x T)
Format:	Paperback
Pages:	247
Edition:	Softcover reprint of the original 3rd ed. 2014
ISBN-13:	978-1-4939-6325-6
Categories:	Books > Medicine > General issues > Medical equipment & techniques > Medical laboratory testing & techniques Books > Medicine > Other branches of medicine > Pathology > Medical microbiology & virology Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical) > General
LSN:	1-4939-6325-2
Barcode:	9781493963256

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