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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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LC-MS/MS in Proteomics - Methods and Applications (Hardcover, 2010)
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LC-MS/MS in Proteomics - Methods and Applications (Hardcover, 2010)
Series: Methods in Molecular Biology, 658
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With the development of new quantitative strategies and powerful
bioinformatics tools to cope with the analysis of the large amounts
of data generated in proteomics experiments, liquid chromatography
with tandem mass spectrometry (LC-MS/MS) is making possible the
analysis of proteins on a global scale, meaning that proteomics can
now start competing with cDNA microarrays for the analysis of whole
genomes. In LC-MS/MS in Proteomics: Methods and Applications,
experts in the field provide protocols and up-to-date reviews of
the applications of LC-MS/MS, with a particular focus on MS-based
methods of protein and peptide quantification and the analysis of
post-translational modifications. Beginning with overviews of the
use of LC-M/MS in protein analysis, the book continues with topics
such as protocols for the analysis of post-translational
modifications, with particular focus on phosphorylation and
glycosylation, popular techniques for quantitative proteomics, such
as multiple reaction monitoring, metabolic labelling, and chemical
tagging, biomarker discovery in biological fluids, as well as novel
applications of LC-MS/MS. Written in the highly successful Methods
in Molecular Biology (TM) series format, chapters include
introductions to their respective subjects, lists of necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and notes on troubleshooting and avoiding
known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in
Proteomics: Methods and Applications presents the techniques and
concepts necessary in order to aid proteomic practitioners in the
application of LC-MS/MS to essentially any biological problem.
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