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RNA Nanotechnology and Therapeutics - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2015)
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RNA Nanotechnology and Therapeutics - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2015)
Series: Methods in Molecular Biology, 1297
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This volume contains a compilation of techniques and laboratory
protocols directly related to RNA nano technology and its
applications in nano biotechnology and nano medicine. The chapters
in this book cover a wide range of research methods that can be
easily comprehended and carried out in a step-by-step manner by
graduate students and postdoctoral fellows from diverse scientific
disciplines. Some of the covered topics include various
biochemical, biophysical and bioinformatics approaches for
analyzing RNA structures and properties; methods to analyze the
multi-step assembly process of RNA nano architectures; purification
of multifunctional RNA nanoparticles by ultracentrifugation and
HPLC; real-time detection of RNA nano constructs in vivo;
conjugation of imaging, targeting and therapeutic modules to RNA
scaffold; and design and characterization of RNA-protein
nanostructures. Written in the highly successful Methods of
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols
and key tips on troubles troubleshooting and avoiding known
pitfalls. Cutting-edge and practical, RNA Nanotechnology and
Therapeutics: Methods and Protocols propels the field toward new
heights by engaging researchers working in the diverse fields of
physical, engineering, and life sciences.
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