Biolistic DNA Delivery - Methods and Protocols (Hardcover, 2013 ed.)

Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 940
Release date:	October 2012
First published:	2013
Editors:	Stephan Sudowe • Angelika B. Reske-Kunz
Dimensions:	254 x 178 x 30mm (L x W x T)
Format:	Hardcover
Pages:	415
Edition:	2013 ed.
ISBN-13:	978-1-62703-109-7
Categories:	Books > Professional & Technical > Biochemical engineering > Biotechnology > Genetic engineering Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > DNA
LSN:	1-62703-109-X
Barcode:	9781627031097

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