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Books > Science & Mathematics > Biology, life sciences > Zoology & animal sciences > Vertebrates > Mammals
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Monoclonal Antibody Protocols (Paperback, 1995 ed.)
Loot Price: R3,008
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Monoclonal Antibody Protocols (Paperback, 1995 ed.)
Series: Methods in Molecular Biology, 45
Expected to ship within 10 - 15 working days
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Since the initial description of techniques to immortalize anti
body-producing B-lymphocytes by fusion with tissue culture-adapted
myeloma cells, methods have been developed to produce monoclonal
antibodies of defined specificity in multiple animal species.
Stable hybrids can be readily produced in mice using a number of
myeloma and hybridoma cell lines. To obviate the problem of
identifying fusion partners in other animal species, xenohybrids
have been produced using B-lymphocytes from the relevant species
and mouse myeloma cells. The use of xenohybrids has minimized the
problem of obtain ing stable antibody-producing hybrids in all
species examined thus far. Although alternative techniques are
being developed to produce monoclonal antibodies by molecular
methods, hybridoma technol ogy will remain the technology of choice
for producing monoclonal antibodies for a variety of applications
in research and industry. The objective of Monoclonal Antibody
Protocols is to provide investigators with a set of methods for
producing and using mono clonal antibodies in biomedical,
agricultural, and biological sciences. The book is not intended to
provide methodology for all possible applications, but rather a
series of methods presented in an easy-- follow format that can be
used by new and established investiga tors, graduate and
postgraduate fellows, and technical staff.
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