sector. This ensured eventual transfer of the technology demonstrated at the wo- shops and Technical Meetings to marketable devices. BIOSET provided assistance for researchers from European laboratories to meet to exchange ideas, use equ- ment, and establish a basis for new joint projects. The secretariat of the Concerted Action BIOSET supported the Technical Meetings. There were three Technical Meetings held, two in Berlin in 1997 and 1998, and the third in Barcelona, in April 2000. The goal of these technical meetings was to join different research and industrial teams to evaluate the performance of their biosensor technology in field conditions with common and standardized surface and waste waters. As a result of these field experiments, the additional information that biosensors can offer to environmental monitoring was also evaluated. Thus, these three Technical Meetings were useful accompanying measures and practical additions to the currently organized yearly workshops. The concerted action BIOSET was f- lowed by the SENSPOL network. The 1st SENSPOL Workshop was held on the 9-11 May 2001 on Sensing Technologies for Contaminated Sites and Groundwater at the University of Alcala. There was one special Workshop on "Genotoxicity Biosensing (TECHNOTOX)" supported by the European Commission DG XII D-1 and BIOSET in the year 2000. The TECHNOTOX meeting at the Flemish Institute for Technological Research (VITO) in Mol was organized by Phillippe Corbisier (VITO), Peter-D. Hansen (TU Berlin) and Damia Barcelo (CSIC Barcelona).

11 11/16 X 8 1/4 in

Antimicrobial Activity of Nanoparticles: Applications in Wound Healing and Infection Treatment presents the state of the art among nanotechnological approaches used in the treatment of infections. This field has gained a large amount of interest over the past few years, in response to the increasing resistance of pathogens to antibiotics. Leading researchers from around the world discuss the synthesis routes of nanobiomaterials, characterization, and their applications as antimicrobial agents. The book covers various aspects: from antiviral and antibacterial nanoparticles, to the functionalization of nanoparticles and their toxicity to human cells. This book offers an advanced reference text for biomedical engineers, materials scientists, clinicians, and biochemists, with an interest in nanomedicine and infection control.

This two-volume work surveys the entire range of general aspects of chromosome research on plants. This first volume is divided into two sections. Section A consists of 11 chapters covering the entire range of general aspects of chromosome research in plants (including a chapter on genetic engineering in crop improvement). Section B is devoted to cytogenetics of cereals and millets (wheat, rye, barley, triticale, oats, maize, rice, pearl millet, and minor millets). More than one chapter is devoted to the same crop to give a detailed treatment of chromosome research (including molecular biology) in these crops.

The second volume deals with cytogenetics of plant materials including legumes, vegetable and oil crops, sugar crops, forage crops, fibre crops, medicinal crops and ornamentals. This work will be useful both as a reference work and a teaching aid to satisfy a wide range of workers. Every chapter has been written by an expert who has been involved in chromosome research on a particular plant material for many years.

The objective of the Springer Handbook of Enzymes is to provide in concise form data on enzymes sufficiently well characterized. Data sheets are arranged in their EC-Number sequence. Each volume comprises one enzyme class, sometimes the enzyme classes have to be divided into several volumes. Considerable progress has been made in enzymology since the publication of the first edition (published as "Enzyme Handbook"): many enzymes are newly classified or reclassified. In the 2nd edition each entry is correlated with references and one or more source organisms. New datafields are created: "application" and "engineering" (for the properties of enzymes where the sequence has been changed). Altogether the amount of data has doubled so that the 2nd edition will consist of 39 volumes plus synonym index. This collection is an indispensable source of information for researchers in biochemistry, biotechnology, organic and analytical chemistry, and food sciences.

There is hardly an area of research developing so quickly and raising so many promises as stem cell research. Adult, embryonic and recently available induced pluripotent stem cells not only foster our understanding of differentiation of endo-, ecto- and mesodermal lineages to all organs of the body, but foremost nourish the hope that cells grown in culture can be used for regeneration of diseased organs such as the heart damaged by myocardial infarction. This book focuses on perspectives of stem cells for regenerative therapy of cardiovascular diseases. Based on the EC consortium INELPY, it reviews the field and disseminates major outcomes of this project. Thus it introduces the reader to this fascinating area of research and incorporates very recent findings interesting to the expert, spanning the field from bench to bedside. The compilation of contributions is unique as there is yet no similar comprehensive overview combining stem cell research with preclinical and clinical evaluation as well as engineering of tissue patches for transplantation. As such it will be an invaluable source of information for all researchers in the stem cell and tissue regeneration field including bioengineers as well as for all clinicians interested in regenerative therapies, especially for ischemic cardiomyopathies.

Advances in Applied Microbiology offers intensive reviews of the latest techniques and discoveries in this rapidly moving field. The editors are recognized experts and the format is comprehensive and instructive.

Membrane techniques are an excellent alternative to traditional methods of purification and separation. This book covers issues related to the most recent developments in the field of membrane techniques. The latest scientific research and their potential applications in industrial solutions are described. In addition, currents trends in food & beverages technologies, and biomedicine are discussed. Moreover, the book emphasizes recent advancements in design of membrane systems, used either for separation or creation of mixtures, from the perspective of industry 4.0 and data management.

Traditional food and bioprocessing technologies are facing challenges due to high expectation from the consumers and producers for better quality and safety, higher process efficiency, and products with novel properties or functionalities. For this reason, in the last few years new forms of physical energies have been explored to propose alternatives to traditional processing technologies. Acoustic energy has the potential to replace or partially substitute conventional processes, and at the same time offer unique opportunities in the characterization of foods and biomaterials. This book is a resource for experts and newcomers in the field of power ultrasound, gives insights into the physical principles of this technology, details the latest advancements, and links them to current and potential applications in the food and bioprocessing related industries.

In the last decade, a number of powerful kernel-based learning methods have been proposed in the machine learning community: support vector machines (SVMs), kernel fisher discriminant (KFD) analysis, kernel PCA/ICA, kernel mutual information, kernel k-means, and kernel ARMA. Successful applications of these algorithms have been reported in many fields, such as medicine, bioengineering, communications, audio and image processing, and computational biology and bioinformatics. ""Kernel Methods in Bioengineering, Signal and Image Processing"" covers real-world applications, such as computational biology, text categorization, time series prediction, interpolation, system identification, speech recognition, image de-noising, image coding, classification, and segmentation. ""Kernel Methods in Bioengineering, Signal and Image Processing"" encompasses the vast field of kernel methods from a multidisciplinary approach by presenting chapters dedicated to adaptation and use of kernel methods in the selected areas of bioengineering, signal processing and communications, and image processing.

This 2nd edition begins with an overview of NMR development and applications in biological systems. It describes recent developments in instrument hardware and methodology. Chapters highlight the scope and limitation of NMR methods. While detailed math and quantum mechanics dealing with NMR theory have been addressed in several well-known NMR volumes, chapter two of this volume illustrates the fundamental principles and concepts of NMR spectroscopy in a more descriptive manner. Topics such as instrument setup, data acquisition, and data processing using a variety of offline software are discussed. Chapters further discuss several routine stategies for preparing samples, especially for macromolecules and complexes. The target market for such a volume includes researchers in the field of biochemistry, chemistry, structural biology and biophysics.

The objective of the Springer Handbook of Enzymes is to provide in concise form data on enzymes sufficiently well characterized. Data sheets are arranged in their EC-Number sequence. Each volume comprises one enzyme class, sometimes the enzyme classes have to be divided into several volumes. Considerable progress has been made in enzymology since the publication of the first edition (published as "Enzyme Handbook"): many enzymes are newly classified or reclassified. In the 2nd edition each entry is correlated with references and one or more source organisms. New datafields are created: "application" and "engineering" (for the properties of enzymes where the sequence has been changed). Altogether the amount of data has doubled so that the 2nd edition will consist of 39 volumes plus synonym index. This collection is an indispensable source of information for researchers in biochemistry, biotechnology, organic and analytical chemistry, and food sciences.

Dynamic Single-Use Bioreactors Used in Modern Liter- and m3- Scale Biotechnological Processes: Engineering Characteristics and Scaling Up, by Christian Loffelholz, Stephan C. Kaiser, Matthias Kraume, Regine Eibl, Dieter Eibl. Orbitally Shaken Single-Use Bioreactors, by Wolf Klockner, Sylvia Diederichs, Jochen Buchs.

Therapeutic Human Cells: Manufacture for Cell Therapy/Regenerative Medicine by Christian van den Bos, Robert Keefe, Carmen Schirmaier, Michael McCaman.

Fast Single-Use VLP Vaccine Productions Based on Insect Cells and the Baculovirus Expression Vector System: Influenza as Case Study by Regine Eibl, Nina Steiger, Sabine Wellnitz, Tiago Vicente, Corinne John, Dieter Eibl.

Microbial High Cell Density Fermentations in a Stirred Single-Use Bioreactor by Thomas Dreher, Bart Walcarius, Ute Husemann, Franziska Klingenberg, Christian Zahnow, Thorsten Adams, Davy de Wilde, Peter Casteels, Gerhard Greller.

Quorus Bioreactor: A New Perfusion-Based Technology for Microbial Cultivation by Sheena J. Fraser, Christian Endres.

Cultivation of Marine Microorganisms in Single-Use Systems by Friederike Hillig, Maciej Pilarek, Stefan Junne, Peter Neubauer.

Flexible Biomanufacturing Processes that Address the Needs of the Future by Bernhard Diel, Christian Manzke, Thorsten Peuker.

An Approach to Quality and Security of Supply for Single-Use Bioreactors by Magali Barbaroux, Susanne Gerighausen, Heiko Hackel.

A Risk Analysis for Production Processes with Disposable Bioreactors by Tobias Merseburger, Ina Pahl, Daniel Muller, Markus Tanner."

Nano-enabled Sustainable and Precision Agriculture is the first single-volume resource to cover this important field using a whole systems approach that considers both opportunities and challenges. The book provides a comprehensive understanding of the role of nanotechnology in agriculture from broad aspects, but also includes a comprehensive view of the interaction of nanomaterials with soil-plant systems. It highlights aspects not described in previous books, including the application of nanoinformatics and artificial intelligence in nano-enabled sustainable agriculture, the application of nanotechnology in alternative forms of agriculture such as hydroponics, and regulatory frameworks for this research field. The book addresses all these aspects by including sections on enhanced sustainability, reduced pollution and enhanced ecosystems' health, and the role of nanoinformatics and machine learning.

This book presents an overview of the ways in which the latest experimental and theoretical nanotechnologies are serving the fields of biotechnology, medicine, and biomaterials. They not only enhance the efficiency of common therapeutics and lower their risks, but thanks to their specific properties, they also provide new capabilities. Nano-scale measurement techniques, such as nano-indentation and nano-scratch methods, could potentially be used to characterize the physical and mechanical properties of both natural tissues and synthetic biomaterials in terms of strength and durability.

Advanced biotechnologies enable breeders to produce a whole generation of new crops for specialist needs (designer crops), including raw materials for the energy, chemical and pharmaceutical industries. This book provides concerns useful to promote an increase of the productivity of crops by using functional genomics (to understand the regulation of plant metabolism at molecular, cellular and whole plants), and the improvement of photosynthetic efficiency (to design new plants with enhanced raw materials percent and recovery). Fundamental thematics have been addressed: metabolic engineering, plant breeding tools, renewable biomass for energy generation, fibres and composites, biopharmaceuticals. The gained know how is relevant to identify bottlenecks in the major production chains and to propose actions for moving these issues forward.

Dr. Jean Huxsoll and a team of distinguished biotechnology industry experts from the U.S. and Europe offer a wealth of practical guidelines to designing, implementing, and managing QA systems to assure that biopharmaceutical products meet standards for safety purity, and potency. Quality Assurance for Biopharmaceuticals covers all important theoretical and practical concerns, including detailed guidelines to meeting GMP compliance; quality assurance of production; quality assurance of analytical methods; advanced documentation, sampling, and validation techniques; comprehensive coverage of regulatory issues in the U.S., Europe, and Japan; and much more.

Until recently, knowledge of ruminant gut microbiology was primarily obtained using classical culture based techniques, which probably only account for 10 to 20% of the rumen microbial population. New gene-based technologies can now be employed to examine microbial diversity through the use of small sub-unit ribosomal DNA analysis (e.g. 16S rDNA) and to understand the function of complex microbial ecosystems in the rumen through metagenomic analysis. These technologies have the potential to revolutionize the understanding of rumen function and will overcome the limitations of classical based techniques, including isolation and taxonomic identification of strains important to efficient rumen function and better understanding of the roles of microorganisms in relation to achieving high productivity and decreasing environmental pollutants. This book presents a comprehensive up-to-date account of the methodologies and protocols for conventional and modern molecular techniques that are currently in use for studying the gut microbial ecology of ruminants. Each chapter has been contributed by experts in the field and methods have been presented in a recipe-like format designed for direct practical use in the laboratory and also to provide insight into the most appropriate techniques, their applications and the type of information that could be expected. The techniques and procedures described are also relevant and adaptable to other gastrointestinal ecosystems and the microbiology of anaerobic environments in general. This manual will a ~demystifya (TM) the methods in molecular microbial ecology for readers who are novice in the field but are excited by the prospects of the technology. Itwould also be invaluable for the experienced workers striving for giving new dimension to their research a" expanding the work in other fields and initiating cross-cutting activities.

This consolidated reference book addresses the various aspects of nano biomaterials used in ophthalmic drug delivery, including their characterization, interactions with ophthalmic system and applications in treatments of the ophthalmic diseases and disorders. In the last decade, a significant growth in polymer sciences, nanotechnology and biotechnology has resulted in the development of new nano- and bioengineered nano-bio-materials. These are extensively explored as drug delivery carriers as well as for implantable devices and scaffolds. At the interface between nanomaterials and biological systems, the organic and synthetic worlds merge into a new science concerned with the safe use of nanotechnology and nano material design for biological applications. For this field to evolve, there is a need to understand the dynamic forces and molecular components that shape these interactions. While it is impossible to describe with certainty all the bio physicochemical interactions at play at the interface, we are at a point where the pockets of assembled knowledge are providing a conceptual framework to guide this exploration, and review the impact on future product development. The book is intended as a valuable resource for academics and pharmaceutical scientists working in the field of polymers, polymers materials for drug delivery, drug delivery systems and ophthalmic drug delivery systems, in addition to medical and health care professionals in these areas.

Altogether, the biochemical, technical and economic limitations on existing proka- otic and eukaryotic expression systems and the growing clinical demand for complex therapeutic proteins have created substantial interest in developing new expression systems for the production of therapeutic proteins. To that end, plants have emerged in the past decade as a suitable alternative to the current production systems, and today their potential for production of high quality, much safer and biologically active complex recombinant pharmaceutical proteins is largely documented. The chapters in this volume, contributed by leaders in the field, sum up the state-- the-art methods for using a variety of different plants as expression hosts for phar- ceutical proteins. Several production platforms are presented, ranging from seed- and leaf-based production in stable transgenic plant lines, to plant cell bioreactors, to viral or Agrobacterium-mediated transient expr ession systems. Currently, antibodies and their derived fragments represent the largest and most important group of biote- nological products in clinical trials. This explains why the potential of most prod- tion platforms is illustrated here principally for antibodies or antibody fragments with acknowledged potential for immunotherapy in humans. In addition, a comparison of different plant expression systems is presented using aprotinin, a commercial phar- ceutical protein, as a test system. Although multiple books and monographs have been recently published on mol- ular pharming, there is a noticeable dearth of bench step-by-step protocols that can be used quickly and easily by beginners entering this new field.

Purification of Laboratory Chemicals: Part One, Physical Techniques, Chemical Techniques, Organic Chemicals, Ninth Edition describes contemporary methods for the purification of chemical compounds. The work includes tabulated methods taken from literature for purifying thousands of individual commercially available chemical substances. To help in applying this information, the more common processes currently used for purification in chemical laboratories and new methods are discussed. For dealing with substances not separately listed, another chapter is included, setting out the usual methods for purifying specific classes of compounds. Laboratory workers, whether carrying out research or routine work, will invariably need to consult this book. Apart from the procedures described, the large amount of physical data about listed chemicals is essential. This fully updated, revised and expanded new edition includes the purification of many new substances that have been available commercially since 2017, along with previously available substances which have found new applications.

This book presents the first comprehensive text on construction biomaterials and bioprocesses. It details aspects of construction biotechnology, a new interdisciplinary area involving applications of environmental and industrial microbiology and biotechnology in geotechnical and civil engineering. It also critically reviews all existing and potential construction biotechnology processes. It discusses a number of topics including the biotechnological production of new construction materials such as self-healing concrete, construction biocomposites, construction bioplastics, and biotechnological admixtures to cement. It also addresses construction-related processes like biocementation, bioclogging, soil surface fixation and biosealing, microbial cements and grouts, the biocoating of construction material surfaces, the microbiology and biosafety of the construction environment, the prevention of biocorrosion as well as biodeterioration and biofouling in civil engineering. Biomediated precipitation of calcium, magnesium, and iron compounds as carbonates, phosphates, sulphides, and silicate minerals in soil for its clogging and strengthening are considered from geotechnical, chemical, and microbiological points of view. It offers an overview of the basic microbiology that will enable civil engineers to perform the construction biogeochemical processes. Design principles and considerations for different field implementations are discussed from a practical point of view. The book can be used as a textbook for graduate and senior undergraduate students in biotechnology, civil engineering and environmental engineering as well as a reference book for researchers and practitioners working in this new interdisciplinary area.

This book explains biosensor development fundamentals. It also initiates awareness in engineers and scientists who would like to develop and implement novel biosensors for agriculture, biomedicine, homeland security, environmental needs, and disease identification. In addition, the book introduces and lays the basic foundation for design, fabrication, testing, and implementation of next generation biosensors through hands-on learning.