This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an "in-situ" PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system's performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid "sample-in-answer-out" testing in routine clinical practice.

Phytochemicals have been present in human diet and life since the birth of mankind, including the consuming of plant foods and the application of herbal treatments. This coevolutionary interaction of plants and people has resulted in humans' reliance on food and medicinal plants as sources of macronutrients, micronutrients, and bioactive phytochemicals. Phytochemicals can be used as adjuvant agents and sensitizers in traditional antibiotic and anticancer therapy, reducing the potential of selecting resistant microbial strains and cancer cells. Recent Frontiers of Phytochemicals addresses the many processes of potential phytochemical evaluation of known sources, with a focus on phytochemical and pharmacological evaluations, and computational research into the structures and pharmacological mechanisms of natural products and their applications in medicine, food and biotech.

OSHA (29 CFR 1910.119) has recognized AIChE/DIERS two-phase flow publications as examples of "good engineering practice" for process safety management of highly hazardous materials. The prediction of when two-phase flow venting will occur, and the applicability of various sizing methods for two-phase vapor-liquid flashing flow, is of particular interest when designing emergency relief systems to handle runaway reactions. This comprehensive sourcebook brings together a wealth of information on methods that can be used to safely size emergency relief systems for two-phase vapor-liquid flow for flashing or frozen, viscous or nonviscous fluids. Design methodologies are illustrated by selected sample problems. Written by industrial experts in the safety field, this book will be invaluable to those charged with operating, designing, or managing today's and tomorrow's chemical process industry facilities.

Bacterial infections cause substantial morbidity and mortality in cancer patients. These infections always remained enigmatic due to initial reluctance of cancer researchers in understanding their etiologic potential. Etiological association of bacteria with cancer gained credibility after discovery of carcinogenic potential of Helicobacter pylori. Moreover, other suspected associations including Salmonella typhi and gallbladder cancer, Streptococcus bovis and colon cancer, Chlamydia psittaci and ocular adnexal lymphoma and Chlamydia pneumoniae with lung cancer, etc. are looking for a legitimate appraisal to unravel their etiologic potential without prejudice. In contrary, bacteria also show protective role in certain types of cancer. Certain agents derived from bacteria are successfully in practice for the management of cancer. The integrate association of bacteria and cancer is evident in both positive and negative aspects. The role of bacteria in cancer etiology and treatment is vigorously studied since last few years. Present book tries to provide current status of research undergoing in above direction, with the glimpses of future possibility for using microbiological knowledge in the management of this deadly killer. This book will interest specialists dealing with cancer associated infectious complications, researchers working in the field of cancer biology, teachers and scientists in the field of microbiology, biotechnology, medicine and oncology. The unique coverage of bacteriology and cancer association in both positive and negative way can usher into development of novel thrust area for microbiology students and experts.

This book addresses the important clinical problem of accurately diagnosing osteoporosis, and analyzes how Bone Turnover Markers (BTMs) can improve osteoporosis detection. In her research, the author integrated microfluidic technology with electrochemical sensing to embody a reaction/detection chamber to measure serum levels of different biomarkers, creating a microfluidic proteomic platform that can easily be translated into a biomarker diagnostic. The Osteokit System, a result of the integration of electrochemical system and microfluidic chips, is a unique design that offers the potential for greater sensitivity. The implementation, feasibility, and specificity of the Osteokit platform is demonstrated in this book, which is appropriate for researchers working on bone biology and mechanics, as well as clinicians.

The critically acclaimed laboratory standard for more than fifty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today truly an essential publication for researchers in all fields of life sciences. This new volume presents methods related to the use of bacterial genetics for genomic engineering. The book includes sections on strain collections and genetic nomenclature; transposons; and phage.

This volume offers an analysis of the scale and nature of the immunological issues facing regenerative medicine, drawing on the expertise of laboratories around the world who have taken up the challenge of applying their expertise in immunology to the vagaries of stem cell biology. In Part I, we explore the extent to which the principles of allograft rejection, learned over several decades from our experiences of whole organ transplantation, apply within the unique context of cell replacement therapy. Part II discusses various innovative ways of addressing the issues of immunogenicity, while, in Part III, we focus exclusively on the induction of immunological tolerance through a variety of novel approaches. It is our hope that this systematic analysis of the current state of the field will galvanise efforts to solve an issue which has so far remained intractable.

DNA nanotechnology: From structure to function presents an overview of various facets of DNA nanotechnology, with a particular focus on their promising applications. This book is composed of three parts. Part I, Elements of DNA Nanotechnology, provides extensive basic information on DNA nanotechnology. Part II, Static and Dynamic DNA Nanotechnology, describes the design and fabrication of static and dynamic DNA nanostructures. Recent advances in DNA origami, DNA walkers and DNA nanodevices are all covered in this part. Part III, Applications of DNA Nanotechnology, introduces a variety of applications of DNA nanotechnology, including biosensing, computation, drug delivery, etc. Together these provide a comprehensive overview of this emerging area and its broad impact on biological and medical sciences. This book is intended for post-graduates, post-doctoral researchers and research scientists who are interested in expanding their knowledge of DNA nanotechnology. It provides readers an impression of the latest developments in this exciting filed.

This thesis describes an in-depth study of an indolizine-based fluorophore, from understanding of its structure-photophysical property relationship to its application as a useful biological reporter. Organic fluorophores have been extensively used in the field of molecular biology owing to their excellent photophysical property, suitable cell permeability, and synthetic flexibility. Understanding of the structure-photophysical property relationship of a given fluorophore often paves the road to the development of valuable molecular probes to visualize and transcribe biological networks. In this thesis, respective chapters deal with molecular design, organic synthesis, structure-property analysis, and quantum-mechanical interpretation of unexplored family of indolizine-based molecules. This systematic exploration has led to rational development of a new microalgae lipid droplet probe, colorful bioorthogonal fluorogenic probes, and a bright mitochondrial probe, working under live cell conditions. Harnessing the optical properties of a given fluorophore has been an important topic for a couple of decades, both in industry and in academia. This thesis provides useful insights for the improvement and development of unique small fluorescent materials, or optical materials.

During the past 15 years, cellular and molecular approaches have emerged as valuable adjuncts to supplement and complement conventional breeding methods for a wide variety of crop plants. Biotechnology increasingly plays a role in the creation, conservation, characterization and utilization of genetic variability for germplasm enhancement. For instance, anther/microspore culture, somaclonal variation, embryo culture and somatic hybridization are being exploited for obtaining incremental improvement in the existing cultivars. In addition, genes that confer insect- and disease-resistance, abiotic stress tolerance, herbicide tolerance and quality traits have been isolated and re-introduced into otherwise sensitive or susceptible species by a variety of transgenic techniques. Together these transformative methodologies grant access to a greater repertoire of genetic diversity as the gene(s) may come from viruses, bacteria, fungi, insects, animals, human beings, unrelated plants or even be artificially derived. Remarkable achievements have been made in the production, characterization, field evaluation and commercialization of transgenic crop varieties worldwide. Likewise, significant advances have been made towards increasing crop yields, improving nutritional quality, enabling crops to be raised under adverse conditions and developing resistance to pests and diseases for sustaining global food and nutritional security. The overarching purpose of this 3-volume work is to summarize the history of crop improvement from a technological perspective but to do so with a forward outlook on further advancement and adaptability to a changing world. Our carefully chosen "case studies of important plant crops" intend to serve a diverse spectrum of audience looking for the right tools to tackle complicated local and global issues.

Sustainable Biotechnology; Sources of Renewable Energy draws on the vast body of knowledge about renewable resources for biofuel research, with the aim to bridge the technology gap and focus on critical aspects of lignocellulosic biomolecules and the respective mechanisms regulating their bioconversion to liquid fuels and other value-added products. This book is a collection of outstanding research reports and reviews elucidating several broad-ranging areas of progress and challenges in the utilization of sustainable resources of renewable energy, especially in biofuels.

Materials from renewable resources are receiving increased attention, as leading industries and manufacturers attempt to replace declining petrochemical-based feedstocks with products derived from natural biomass, such as cereal straws. Cereal straws are expected to play an important role in the shift toward a sustainable economy, and a basic knowledge of the composition and structure of cereal straw is the key to using it wisely. Cereal Straw as a Resource for Sustainable Biomaterials and Biofuels: Chemistry, Extractives, Lignins, Hemicelluloses and Cellulose provides an introduction to straw chemistry. Topics discussed include the structure, ultrastructure, and chemical composition of straw; the structure and isolation of extractives from the straw; the three main components of straw: cellulose, hemicelluloses, and lignins; and chemical modifications of straw for industrial applications. This book will be helpful to scientists interested in the areas of natural resource management, environmental chemistry, plant chemistry, material science, polysaccharide chemistry, and lignin chemistry. It will also be of interest to academic and industrial scientists/researchers interested in novel applications of agricultural residues for industrial and/or recycling technologies.

Animal cell technology is a growing discipline of cell biology which aims not only to understand the structure, function and behavior of differentiated animal cells, but also to ascertain their ability to be used for industrial and medical purposes. Some of the major goals of animal cell technology include: the clonal expansion of differentiated cells, the optimization of their culture conditions, modulation of their ability for the production of medically and pharmaceutically important proteins and the application of animal cells to gene therapy, artificial organs and functional foods. This volume gives the readers a complete review of the present state-of-the-art research in Japan and other countries where this field is well advanced. The Proceedings will be useful to cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and to those working in either academic environments or in the biotechnology and pharmacy industries related to animal cell culture.

The entire range of the developmental processes in plants is regulated by a shift in the hormonal concentration, tissue sensitivity and their interaction with the factors operating around them. Out of the recognized hormones, attention has largely been focused on five - Auxins, Gibberellins, Cytokinin, Abscisic acid and Ethylene. However, the information about the most recent group of phytohormone (Brassinosteroids) has been incorporated in this book. This volume includes a selection of newly written, integrated, illustrated reviews describing our knowledge of Brassinosteroids and aims to describe them at the present time. Various chapters incorporate both theoretical and practical aspects and may serve as baseline information for future researches through which significant developments are possible. This book will be useful to the students, teachers and researchers, both in universities and research institutes, especially in relation to biological and agricultural sciences.

There is hardly a technical library in the world in which the volumes of the Chemical Formulary (Volumes 1-34) do not occupy a prominent place. Chemists both experienced and beginner, continually refer to them. It does not duplicate any of the formulas included in previous volumes, but lists a wide array of modern and salable products from all branches of the chemical industries. An excellent reference for formulation problems. -CONTENTS - I. Introduction - II. Adhesives - III. Coatings - IV. Cosmetics - V. Detergents and Disinfectants - VI. Drug Products - VII. Elastomers, Plastics and Resins - VIII. Foods and Beverages - IX. Polishes - X. Textile Specialties - XI. Miscellaneous - Appendix - Index - PREFACE - Chemistry, as taught in our schools and colleges, concerns chiefly synthesis, analysis, and engineering-and properly so. It is part of the right foundation for the education of the chemist. Many a chemist entering an Industry soon finds that most of the products manufactured by his concern are not synthetic or definite complex compounds, but are mixtures, blends, or highly complex compounds of which he knows little or nothing. The literature in this field, if any, may be meager, scattered, or obsolete. Even chemists with years of experience In one or more Industries spend considerable time and effort in acquainting themselves with any new field which they may enter. Consulting chemists similarly have to solve problems brought to them from industries foreign to them. There was a definite need for an up-to-date compilation of formulae for chemical compounding and treatment. Since the fields to be covered are many and varied, an editorial board of chemists and engineers engaged in many industries was formed. Many publications, laboratories, manufacturing firms, and Individuals have been consulted to obtain the latest and best information. It is felt that the formulas given in this volume will save chemists and allied workers much time and effort. Manufacturers and sellers of chemicals will find, In these formulae, new uses for their products. Non-chemical executives, professional men, and Interested laymen will make through this volume a "speaking acquaintance" with products which they may be using, trying or selling. It often happens that two Individuals using the same Ingredients in the same formula get different results. This may be due to slight deviations in the raw materials or unfamiliarity with the intricacies of a new technique. Accordingly, repeated experiments may be necessary to get the best results. Although many of the formulas given are being used commercially, many have been taken from the literature and may be subject to various errors and omissions. This should be taken into consideration. Wherever possible, it is advisable to consult with other chemists or technical workers regarding commercial production.

Acknowledgements - Introduction - Contents - Part One- Natural Rubber - 1. THE STORY OF NATURAL RUBBER - The early history - The beginnings of the rubber industry - Goodyear and vulcanization - Plantation rubber - 2. THE NATURE OF NATURAL RUBBER - The physical properties of natural rubber- Tensile properties - Dynamic properties - Hardness - Abrasion - Electrical properties - The chemistry of natural rubber - Atoms and molecules - The formula of natural rubber - The elasticity of natural rubber - Part Two-Synthetic Rubber - 3. HISTORICAL INTRODUCTION TO SYNTHETIC RUBBER - The beginnings of synthetic rubber production - Synthetic rubber in the First World War - Progress between the wars - The American contribution - Developments after the Second World War - 4. THE MANUFACTURE OF GENERAL PURPOSE SYNTHETIC RUBBER - Butadiene: Petroleum - Butadiene and cracking - Styrene Production of the polymer: Emulsion polymerization - The polymerization formula - The synthetic rubber plant - 5. THE PROCESSING OF GENERAL PURPOSE SYNTHETIC RUBBER - Processing machinery: The bale-cutting machine - The mill - The internal mixer - The calendar - The spreading machine - The extruder Compounding: Plasticizers and softeners - Tack - Extenders - Reclaimed rubber - Fillers - Colouring materials - The ageing of rubber - Antioxidants - Vulcanization Accelerators - Vulcanization activators - Summary of compounding 6. SPECIAL PURPOSE RUBBERS - Nitrile rubber - Butyl rubber - Neoprene - Thiokol - Silicone rubbers - Polyurethanes - Hard rubber - 7. THE MANUFACTURE OF RUBBER ARTICLES - Mechanicals: Soles and heels - Bathing caps - Hot water bottles - Extruded articles - Wires and cables - Hose: Plain hose - Wrapped hose - Moulded hose - Armouring - Belting: Conveyor belts - Transmission belting - Rubber balls: Gold balls - Tyres: The cover - The bead - The casing - The tread and sidewalls - Cover building - Vulcanizing the cover - Inner tubes - Goods from latex: Compounding latex - Dipped goods - Latex thread - Latex foam - Part Three-The Future of Rubber - 8. MODERN DEVELOPMENTS - Polymerization: Condensation polymerization - Addition polymerization - Initiators - The arrangement of atoms in a chain - Synthetic natural rubber - cis Polybutadiene - Radiation and rubber: Polymerization - Cross-linking - Looking ahead - Bibliography - Glossary - Index - Plates - The object of this book is to explain what these various rubbers are, how they behave, and why they behave as they do. As synthetic rubbers are now being made in this country and will play an important part in the future of the rubber industry, most of this book is devoted to them. How they are made and how they compare with natural rubber is discussed in the appropriate place.- To enable a reasonable comparison to be made between natural rubber and the various synthetic rubbers the subject has been treated from a scientific standpoint, and to keep the size of the book between reasonable limits much technological information has been omitted. Because of the comparison between natural and synthetic rubbers the first section of this book is devoted to natural rubber. In this section the fundamentals of rubber science are introduced, to be developed later in connection with the synthetic rubbers. This book is intended as an introduction to a complex subject, and as a surveyor report for non-technical readers who wish to know something about rubber. The reader who requires further information on a particular point or topic should consult the bibliography at the end of the book.-

The emergence of nanotechnology has had a profound effect on almost every aspect of the 21st century's daily life. It has had a revolutionary impact from stain-resistant clothing and cosmetics to environmental issues , including energy and medicine and even aerospace engineering. In Oxidative Stress and Nanotechnology: Methods and Protocols, expert researchers in the field detail various aspects of nanotechnology from the oxidative stress point of view. Focusing on synthesis of different antioxidant nanoparticles and antioxidant-loaded nanoparticles, as well as their in vitro/ in vivo mechanisms of action along with their clinical relevance. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Oxidative Stress and Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of techniques for nanotechnology in pathophysiology and protection from the oxidative stress point of view.

Various types of secondary agriculture and forestry wastes represent valuable resource materials for developing alternate energy as biofuels and other value added products such as sugars, phenols, furans, organic acids, enzymes and digestible animal feed etc. However, if not managed properly, waste material and environmental contaminants generated by various industries such as food and feed, pulp and paper and textile may lead to severe environmental pollution. The energy, food and feed demand necessitate developing simple and economically viable technologies for environmental management and resource recovery. Microorganisms and their enzymes contribute significantly in utilization of plant residues, resource recovery and eventually in pollution mitigation. "Biotechnology for Environmental Management and Resource Recovery" presents a comprehensive review of selected research topics in a compendium of 16 chapters related to environmental pollution control and developing biotechnologies in agro-ecosystem management and bioconversion of agro-residues (lignocellulosics) into biofuels, animal feed and paper etc. This book provides a valuable resource for reference and text material to graduate and postgraduate students, researchers, scientists working in the area of microbiology, biotechnology, and environmental science and engineering.

Light Microscopic Analysis of Mitochondrial Heterogeneity in Cell Populations and Within Single Cells, by S. Jakobs, S. Stoldt, and D. Neumann
*

Advanced Microscopy of Microbial Cells, by J. A. J. Haagensen, B. Regenberg, and C. Sternberg

*

Algebraic and Geometric Understanding of Cells, Epigenetic Inheritance of Phenotypes Between Generations, by K. Yasuda

*

Measuring the Mechanical Properties of Single Microbial Cells, by C. R. Thomas, J. D. Stenson, and Z. Zhang
*

Single Cell Analytics: Pushing the Limits of the Doable,
by H. Kortmann, L.M. Blank, and A. Schmid

*
Cultivation-Independent Assessment of Bacterial Viability, by F. Hammes, M. Berney, and T. Egli
*

Resolution of Natural Microbial Community Dynamics by Community Fingerprinting, Flow Cytometry and Trend Interpretation Analysis, by P. Bombach, T. Hubschmann, I. Fetzer, S. Kleinsteuber, R. Geyer, H. Harms, and S. Muller
*Multivariate Data Analysis Methods for the Interpretation of Microbial Flow Cytometric Data, by

H.M. Davey, and C.L. Davey

*

From Single Cells to Microbial Population Dynamics:

Modelling in Biotechnology Based on Measurements of Individual Cells, by T. Bley"

"Plant and Process Engineering 360" will be the backbone of any plant, chemical, or process engineer s library. This is a broad area in which engineers need to be familiar with a wide array of techniques, technologies andequipment. Its focus on providing a broad introduction to key systems make the book the first point of reference for engineers who are involved with designing, specifying, maintaining or working with plant, process and control technologies in many sectors, includingmanufacturing, chemical process, and energy.
A single-source of plant and processequipment information for engineers, providinga 360 degree view of the critical equipment engineers encounterEnablesreaders to get up to speed with unfamiliar topics quickly withan overview of important but disparate technologies that are specific to plant engineering Coversthe systems and processes that drive effective and efficient plants and processesDrawn from authoritative Elsevier resources, this book is a first port of call with breadth and depth of content, from leading figures in the field."

This books provides a compendium of electrospinning strategies and related technologies for the production of biomaterials for tissue engineering and regenerative medicine applications. It gives a broad overview of the field as well as cutting-edge research on electrospinning and how it is applied to engineer biomaterials. This is an ideal book for biomaterials scientists, engineers, students, and researchers. This book also: Presents cutting-edge research performed in the area of electrospinning with applications in tissue engineering and regenerative medicine Provides readers from the biomaterials field as well as those new to the field with a broad overview of the multiple applications of electrospun biomaterials Summarizes the latest research from the past ten years on electrospinning and related technologies

This book summarizes the recent advancements for drug delivery systems (DDS) in terms of fundamental principles, rapidly emerging techniques and developing frontiers of molecular imprinting. Especially with the combination of enantioselective molecularly imprinted polymers and water compatible molecularly imprinted polymers, stimuli responsive imprinted DDS have been innovated and applied to dermal delivery, ophthalmic drugs and cancer treatment. This philosophy comprehensively revolutionizes the treatment strategy of human healthcare and provides the possibility to re-trigger in vivo an exhaust system after the complete release of the starting drug cargo, thus enabling precision medicine. To this end, the following unique features will be discussed and concluded: 1) State-of-the-art definition of MIP as drug delivery systems. 2) Advanced techniques and clinical applications of MIP as drug delivery systems in the past decade. 3) Novel frontiers and brand-new technologies, for example, drug delivery devices for zero-order sustained release and stimuli responsive imprinted DDS. 4) Revolutionary impact on dermal delivery, ophthalmic drugs and cancer treatment. 5) Future challenges and perspectives

The Fast Pyrolysis Handbook Volume 2 is an edited version of the final report of the European Commission and IEA Bioenergy sponsored Pyrolysis Network that officially finished in 2001. It provides a companion volume to the first handbook published in 1999 and it is again intended that this will provide a useful guide both to newcomers to the subject area as well as those already involved in research, development and implementation. A significant feature of this second volume is the greater attention paid to addressing commercial issues such as marketability, transportation and safety. Fast pyrolysis is a high temperature process in which biomass is rapidly heated in the absence of oxygen. As a result it decomposes to generate mostly vapours and aerosols and some charcoal. After cooling and condensation, a dark brown mobile liquid is formed which has a heating value about half that of conventional fuel oil. While it is related to the traditional pyrolysis processes for making charcoal, fast pyrolysis is an advanced process that is carefully controlled to give high yields of liquid. The essential features of a fast pyrolysis process are: very high heating and heat transfer rates, which usually requires a finely ground biomass feed; carefully controlled pyrolysis reaction temperature of around 500C in the vapour phase, with short vapour residence times of typically less than 2 seconds; rapid cooling of the pyrolysis vapours to give the main product - bio-oil.

Appropriate for a wide range of disciplines, from biology to non-biology, law and nursing majors, "DNA and Biotechnology" uses a straightforward and comprehensive writing style that gives the educated layperson a survey of DNA by presenting a brief history of genetics, a clear outline of techniques that are in use, and highlights of breakthroughs in hot topic scientific discoveries.

Engaging and straightforward scientific writing style Comprehensive forensics chapter Parallel Pedagogic material designed to help both readers and teachers. Highlights in the latest scientific discoveries Outstanding full-color illustration that walk reader through complex concepts