This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.

This is the fourth updated and revised edition of a well-received book that emphasises on fungal diversity, plant productivity and sustainability. It contains new chapters written by leading experts in the field. This book is an up-to-date overview of current progress in mycorrhiza and association with plant productivity and environmental sustainability. The result is a must hands-on guide, ideally suited for agri-biotechnology, soil biology, fungal biology including mycorrrhiza and stress management, academia and researchers. The topic of this book is particularly relevant to researchers involved in mycorrhiza, especially to food security and environmental protection. Mycorrhizas are symbioses between fungi and the roots of higher plants. As more than 90% of all known species of plants have the potential to form mycorrhizal associations, the productivity and species composition and the diversity of natural ecosystems are frequently dependent upon the presence and activity of mycorrhizas. The biotechnological application of mycorrhizas is expected to promote the production of food while maintaining ecologically and economically sustainable production systems.

This book gives a comprehensive overview of electrochemical-based biosensors and their crucial components. Practical examples are given throughout the text to illustrate how the performance of electrochemical-based biosensors can be improved by nanoscale surface modification and how an optimal design can be achieved. All essential aspects of biosensors are considered, including electrode functionalization, efficiency of the mass transport of reactive species, and long term durability and functionality of the sensor. This book also: * Explains how the performance of an electrochemical-based biosensor can be improved by nanoscale surface modification * Gives readers the tools to evaluate and improve the performance of a biosensor with a multidisciplinary approach that considers electrical, electrostatic, electrochemical, chemical, and biochemical events * Links the performance of a sensor to the various governing physical and chemical principles so readers can fully understand how a biosensor with nanoscale modified electrode surface functions.

Computer-aided process engineering (CAPE) plays a key design and operations role in the process industries, from the molecular scale through managing complex manufacturing sites. The research interests cover a wide range of interdisciplinary problems related to the current needs of society and industry. ESCAPE 23 brings together researchers and practitioners of computer-aided process engineering interested in modeling, simulation and optimization, synthesis and design, automation and control, and education. The proceedings present and evaluate emerging as well as established research methods and concepts, as well as industrial case studies.
Contributions from the international community using computer-based methods in process engineeringReviews the latest developments in process systems engineering

Emphasis on industrial and societal challenges

Ion-exchange Technology I: Theory and Materials describes the theoretical principles of ion-exchange processes. More specifically, this volume focuses on the synthesis, characterization, and modelling of ion-exchange materials and their associated kinetics and equilibria. This title is a highly valuable source not only to postgraduate students and researchers but also to industrial R&D specialists in chemistry, chemical, and biochemical technology as well as to engineers and industrialists.

This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques.

The purpose of this book is to assess the potential effects of biotechnological approaches particularly genetic modification on biodiversity and the environment. All aspects of biodiversity such as ecological diversity, species diversity and genetic diversity are considered. Higher organisms contain a specific set of linear DNA molecules called chromosomes and a complete set of chromosomes in an organism comprises its genome. The collection of traits displayed by any organism (phenotype) depends on the genes present in its genome (genotype). The appearance of any specific trait also will depend on many other factors, including whether the gene(s) responsible for the trait is/are turned on (expressed) or off, the specific cells within which the genes are expressed and how the genes, their expression and the gene products interact with environmental factors. The primary biotechnology which concerns us is that of genetic manipulation, which has a direct impact on biodiversity at the genetic level. By these manipulations, novel genes or gene fragments can be introduced into organisms (creating transgenics) or existing genes within an organism can be altered. Transgenics are a major area of concern, combining genes from different species to effectively create novel organisms. Current rates of disappearance of biological and cultural diversity in the world are unprecedented. Intensive resource exploitation due to social and economic factors has led to the destruction, conversion or degradation of ecosystems. Reversing these trends requires time to time assessment to integrate conservation and development.

This book describes a new family of bio-polymer gels made from cytoskeletal proteins - actin, microtubule, and tropomyosin. The importance of the gel state with multi-scale hierarchical structure is emphasized to utilize emergent functions in living organisms. Detailed protocol of gel preparation, specified method of structure investigation, and dynamic studies of self-organization, self-healing, synchronized oscillating, and autonomous motility functions are introduced together with biomimetic functions of synthetic hydrogels.

The rapidly expanding molecular biological techniques and approaches have significant impact on microbial biotechnology, hence the need for the addition of four new chapters in the third edition of this textbook - "Chapter 3: Application of 'Omics' Technologies in Microbial Fermentation", "Chapter 5: Microbial Genome Mining for Identifying Antimicrobial Targets", "Chapter 21: Bacterial Biofilm: Molecular Characterization and Impacts on Water Management" and "Chapter 23: Microbial Biomining". "Chapter 15: Transgenic Plants" has been completely revised while most of the other chapters have been thoroughly updated in this new edition.There already exist a number of excellent general textbooks on microbiology and biotechnology that deal with the basic principles of microbial biotechnology. To complement them, this book focuses on the various applications of microbial-biotechnological principles. A teaching-based format is adopted, whereby working problems, as well as answers to frequently asked questions, supplement the main text. The book also includes real life examples of how the application of microbial-biotechnological principles has achieved breakthroughs in both research and industrial production.Although written for polytechnic students and undergraduates, the book contains sufficient information to be used as a reference for postgraduate students and lecturers. It may also serve as a resource book for corporate planners, managers and applied research personnel.

"Transforming Your STEM Career Through Leadership and Innovation" offers valuable information on what it means to be a leader and innovator and encourages you to discover and develop these skills for yourself. This book integrates leadership and innovation principles with personal examples and profiles of inspirational women. By providing a clear process on how to build upon your personal strengths to realize leadership and innovation goals, this book will inspire you to pick up the mantle and meet the critical need for leadership and innovation in the STEM fields. This is a must-have guide that is relevant and valuable for women in all stages of their careers.
Examines research-based leadership and innovation principles to make these critically important characteristics both real and attainableEmpowers you to build upon your own strengths and successes to discover and develop leadership and innovation skillsFeatures a companion website that highlights women s leadership success stories, innovation resources and best practicesProvides a practical guide that educates, encourages and equips you to pursue leadership and innovation opportunities
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"Synthetic Biology" provides a framework to examine key enabling components in the emerging area of synthetic biology. Chapters contributed by leaders in the field address tools and methodologies developed for engineering biological systems at many levels, including molecular, pathway, network, whole cell, and multi-cell levels. The book highlights exciting practical applications of synthetic biology such as microbial production of biofuels and drugs, artificial cells, synthetic viruses, and artificial photosynthesis. The roles of computers and computational design are discussed, as well as future prospects in the field, including cell-free synthetic biology and engineering synthetic ecosystems.

Synthetic biology is the design and construction of new biological entities, such as enzymes, genetic circuits, and cells, or the redesign of existing biological systems. It builds on the advances in molecular, cell, and systems biology and seeks to transform biology in the same way that synthesis transformed chemistry and integrated circuit design transformed computing. The element that distinguishes synthetic biology from traditional molecular and cellular biology is the focus on the design and construction of core components that can be modeled, understood, and tuned to meet specific performance criteria and the assembly of these smaller parts and devices into larger integrated systems that solve specific biotechnology problems.
Includes contributions from leaders in the field presents examples of ambitious synthetic biology efforts including creation of artificial cells from scratch, cell-free synthesis of chemicals, fuels, and proteins, engineering of artificial photosynthesis for biofuels production, and creation of unnatural living organismsDescribes the latest state-of-the-art tools developed for low-cost synthesis of ever-increasing sizes of DNA and efficient modification of proteins, pathways, and genomesHighlights key technologies for analyzing biological systems at the genomic, proteomic, and metabolomic levels which are especially valuable in pathway, whole cell, and multi-cell applicationsDetails mathematical modeling tools and computational tools which can dramatically increase the speed of the design process as well as reduce the cost of development. "

Among the most promising techniques to handle small objects at the micrometer scale are those that employ electrical forces, which have the advantages of voltage-based control and dominance over other forces. The book provides a state-of-the-art knowledge on both theoretical and applied aspects of the electrical manipulation of colloidal particles and fluids in microsystems and covers the following topics: dielectrophoresis, electrowetting, electrohydrodynamics in microsystems, and electrokinetics of fluids and particles. The book is addressed to doctoral students, young or senior researchers, chemical engineers and/or biotechnologists with an interest in microfluidics, lab-on-chip or MEMS.

The purpose of "Ligand Efficiency Indices for Drug Discovery: Towards an Atlas-Guided Paradigm" is to introduce in a concise and self-contained form the concepts, ideas, applications and examples of efficiency-driven drug discovery to the biomedical community at large. The book emphasizes the use of 'new variables' and more objective numerical methods to drive drug discovery in an encompassing way. These 'new variables' are based on Ligand Efficiency Indices (LEIs) formulated in a way that permits mapping Chemico-Biological Space (CBS) in an Atlas-like representation. It provides a practical and timely discussion of the concepts, ideas, applications and examples of efficiency-driven drug discovery. This book emphasizes the use of a graphical representation and objective numerical methods to drive drug discovery more effectively. It presents the definition of LEIs and the corresponding efficiency planes within an atlas-like environment to provide a robust graphical and numerical framework for medicinal chemists and drug-discoverers.
Provides a practical and timely discussion of the concepts, ideas, applications and examples of efficiency-driven drug discoveryEmphasizes the use of new variables and more objective numerical methods to drive quicker and more effective drug discoveryPresents the definition of Ligand Efficiency Indices (LEIs) and the corresponding efficiency planes as key concepts to provide a graphical and numerical framework"

In" Quantum Dots: Applications in Biology, Second Edition, "expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots (QDs) as fluorescent probes in Life Sciences. While the second section covers important features about QDs preparative processes and characterizations for their successful application as fluorophores. The third part presents main aspects related to QDs methods applied to live cells and tissues. The fourth section focuses on QDs experiments in small animals and the fifth part demonstrates the versatility of QDs in a set of FRET applications. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, In" Quantum Dots: Applications in Biology, Second Edition" aids scientists in continuing to study QDs by providing information about methods and protocols helping to expand their research."

Biotechnology: Prospects and Applications covers the review of recent developments in biotechnology and international authorship presents global issues that help in our understanding of the role of biotechnology in solving important scientific and societal problems for the benefit of mankind and environment. A balanced coverage of basic molecular biology and practical applications, relevant examples, colored illustrations, and contemporary applications of biotechnology provide students and researchers with the tools and basic knowledge of biotechnology. In our effort to introduce students and researchers to cutting edge techniques and applications of biotechnology, we dedicated specific chapters to such emerging areas of biotechnology as Emerging Dynamics of Brassinosteroids Research, Third generation green energy, Bioremediation, Metal Organic Frameworks: New smart materials for biological application, Bioherbicides, Biosensors, Fetal Mesenchymal Stem Cells and Animal forensics. Biotechnology: Prospects and Applications will be highly useful for students, teachers and researchers in all disciplines of life sciences, agricultural sciences, medicine, and biotechnology in universities, research stations and biotechnology companies. The book features broader aspects of the role of biotechnology in human endeavor. It also presents an overview of prospects and applications while emphasizing modern, cutting-edge, and emerging areas of biotechnology. Further, it provides the readers with a comprehensive knowledge of topics in food and agricultural biotechnology, microbial biotechnology, environmental biotechnology and animal biotechnology. The chapters have been written with special reference to the latest developments in above broader areas of biotechnology that impact the biotechnology industry. A list of references at the end of each chapter is provided for the readers to learn more about a particular topic. Typically, these references include basic research, research papers, review articles and articles from the popular literature.

If you investigate biological systems and might use mass spectrometry in your research but need to know more about it, this book is for you. It introduces the fundamental concepts of mass spectrometry and how mass spectrometers work. It also presents recent advancements particularly interesting to bio-researchers in an easy-to-understand manner that does not require extensive background in chemistry, math, or physics.
Key Features
* Glossary of basic terms
* Abundant illustrations
* Examples of applications
* Practical tips on using mass spectrometric techniques
* Useful for peptide, protein, oligonucleotide, and carbohydrate analysis
* Simplified description of mass spectrometry including:
* Matrix-Assisted Laser Desorption/Ionization (MALDI)
* Electrospray Ionization (ESI)
* Fast Atom/Ion Bombardment (FAB)

Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering.

This six volume book set examines a range of topics and applications related to biotechnology. Volumes include fermentation and algal biotechnologies; agricultural biotechnology; medical biotechnology, biopharmaceutics; biosafety, bioethics, biotechnology policy; microbiomes; bioenergy and environmental biotechnology for sustainable development. The topics address significant aspects of the dairy and fermented foods; crop genetics, breeding and genomics; microalgae and novel products; molecular diagnostics and DNA forensics; biosafety, bioethics and legal issues in biotechnology; biotechnology policy advocacy, enlightenment and engagement with stakeholders; medical and pharmaceutical biotechnology; omics and bioinformatics; waste recycling, biofuels and environmental remediation; animal genetics, breeding and genomics; genetic resources conservation and utilization; medicinal and underutilized plants; medical insect biotechnology, genomics and molecular genetics of pests; microbiomes and microbial biotechnology; biotechnology education and curriculum development; and water and waste water research. Volumes include: I: Fermentation and Algal Biotechnologies for the Food, Beverage and other Bioproduct Industries II: Agricultural Biotechnology, Biodiversity and Bio-resources Conservation and Utilization III: Medical Biotechnology, Biopharmaceutics, Forensic Science and Bioinformatics IV: Biosafety and Bioethics in Biotechnology: Policy, Advocacy, and Capacity Building V: Microbiomes and Emerging Applications VI: Bioenergy and Environmental Biotechnology for Sustainable Development The book is a timely knowledge product that documents key issues on advances in biotechnology for use by a variety of readers including postgraduate students, professionals in the field, policy makers, science advocacy groups.

This book is intended to give technological background and practical examples, but also to give general insight into the on-going technology development in the area of biodetection. The content is therefore suitable for an array of stakeholders (decision makers, purchasing officers, etc.) and end-users of biodetection equipment within the areas of health, environment, safety and security, and military preparation. The book is divided into three sections. The first section discusses the fundamental physical and biological properties of bioaerosol's. The second section goes into more detail and discusses in-depth the most commonly used detection principles. The third section of the book is devoted to technologies that have been used in standoff applications. The last section of the book gives an overview of trends in bioaerosol detection. The reader of this book will gain knowledge about the different biodetection technologies and thus better judge their capabilities in relation to desired applications.