This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.

Building Biotechnology helps readers start and manage biotechnology companies and understand the business of biotechnology. This acclaimed book describes the convergence of scientific, policy, regulatory, and commercial factors that drive the biotechnology industry and define its scope. In addition to its popularity among business professionals and scientists seeking to apply their skills to biotechnology, Building Biotechnology has also been adopted as a course text in dozens of advanced biotechnology programs. This fourth edition significantly expands upon the foundation laid by the first three, updating case law and business models in this dynamic industry and adding significantly more case studies, informative figures and tables. Most importantly, Building Biotechnology enables seasoned business professionals and entrepreneurial scientists alike to understand the drivers of biotechnology businesses and apply their established skills for commercial success.

th We compiled this volume mostly from presentations at the 6 International Plant Cold Hardiness Seminar (PCHS) after consulting with Professor Tony H. H. Chen, Oregon State University, USA, Professor Pekka Heino, University of Helsinki, Finland, th and Dr. Gareth J. Warren, University of London, Surrey, UK. The 6 International PCHS was held at the Unitas Congress Center, Helsinki, Finland from July 1-5, 2001. There were 110 registered scientists at the serttinar representing 20 countries: Australia, Belgium, Canada, Chile, the Czech Republic, Denmark, Estonia, Finland, Gennany, Hungary, Iceland, Italy, Japan, Norway, Poland, Spain, Sweden, Taiwan, United Kingdom, and United States of America. The infonnation compiled represents the state of the art of research in phmt cold hardiness in tenns of gene regulation, gene expression, signal transduction, the physiology of cold hardiness and, ultimately, the genetic engineering for cold tolerant plants. The International PCHS was initiated in 1977 at the University of Minnesota, St. Paul, Minnesota. It has been traditionally held at 5-year intervals at various locations. th Because of the rapid advances of research in plant cold hardiness, attendees at the 6 meeting unanimously adopted a resolution to hold the seminar in 3-year intervals instead of 5 in the future. Consequently, the next seminar will be held in 2004 in Sapporo, Japan, and Professor Seizo Fujikawa from Hokkaido University will serve as the host.

The tobacco BY-2 cell system is a unique model cell line for the study of dynamic features of plant cells. As extension of Volume 53, Tobacco BY-2 Cells, which presented basic aspects of the cell system, this present volume provides a wealth of new approaches. Topics include: various aspects of the cell cycle and cellular dynamics using BY-2 cells; physiological and developmental aspects of BY-2 cells; recent developments in the knowledge of intracellular traffic of BY-2 cells; BY-2 cells as hosts for infectious diseases; dynamic features of mitochondrial fusion and division; BY-2 cells as tools to elucidate the biosynthesis of isoprenoids; recent developments in the omics of BY-2 cells; and novel techniques for handling BY-2 cells. This latest volume in the series is an invaluable source of information for scientists in basic and applied plant biology.

Arbuscular Mycorrhiza (AM) is the most common mycorrhizal type involved in agricultural systems, and the most widespread plant root symbiosis. The fungi involved (Glomales) are known to promote plant growth and health by acting as biofertilizers, bioprotectors and bioregulators. The main aim of this book is to provide readers with theoretical and applied knowledge essential for the use of AM fungi in improving plant health and fitness, production of high quality food and in conservation of natural resources. The different chapters target understanding the role of AM fungi in sustainable crop production, discussing ways to improve biological equilibria between microorganisms in the mycorrhizosphere, analysing genetic, physiological, cellular and molecular bases of AM functioning and establishing technologies for inoculum production, according to the regulatory guidelines for application.

This book summarizes the current status of research on bilayer lipid membranes (planar lipid bilayers and spherical liposomes). In addition to describing the properties of lipid bilayers and examining biomembrane phenomena, the book has two other objectives. The first is to present practical methods for the formation and study of lipid bilayers with either aqueous or metal-lipid bilayer interfaces. The second aim is to treat planar lipid bilayers as a new type of interfacial adsorption phenomena. The first nine chapters cover properties of biomembranes, basic principles of membrane biophysics, transport, electrochemistry, physiology, bioenergetics, and photobiology. Chapter 10 presents the following topics: lipid bilayers in medicine, supported lipid bilayers as sensors, a short discussion of liposomes, and solar energy transduction via semiconductor septum photovoltaic cells based on natural photosynthesis.

Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 1: Optical-Based Detectors delves into direct and indirect optical detectors, including methods involving surface plasmon resonance, interferometric sensors, CCD based detectors, and spectrometers, among many other cutting-edge technologies. Written in the highly successful Methods in Molecular Biologya" series format, chapters include brief introductions to the subjects, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.

Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.

This book is a compilation of the engineering data on mixing, which have appeared in the major technical journals of chemical engineering and bioengineering since 1975. That year marked the beginning of a period of rapid advancement in the science and technology of mixing, with rather reliable results for both theoretical and experimental studies. In addition, some important earlier articles which have been, and still are being referred to, are included.
Designs of both agitators and tanks still depend primarily on art and experience. In light of this it was felt that the data on mixing should be compiled and presented in a systematic manner to assist in design and analysis of agitated tanks, and to provide easier access to mixing data for various engineering activities. Although computer-aided searches of pertinent data bases can be of assistance to chemical engineers and bioengineers in their studies, they are sometimes time-consuming and often costly. Furthermore inadequate selection of key words can jeopardize the searches. This book offers an alternative method of surveying mixing data which interests readers.
The first chapter presents a variety of results for the experimental measurements of flow patterns in stirred tanks. Most of the measurements were made by using modern Laser-Doppler techniques. This chapter is useful for the prediction of flow patterns in tanks with many different geometries, various types of agitators, and fluids of diverse physical and rheological properties, plus valuable data for the validation of results obtained by CFD simulations. Chapters 2 through 5 deal with data for traditional chemical engineering subjects and Chapter 6 summarizes a number of scale-up relations developed over the years for various systems. These include liquid, solid-liquid, liquid-liquid, gas-liquid, and solid-liquid-gas systems. Chapter 7 provides data related to multiphase processes, and most importantly, drop size and drop-size distributions and bubble-size distributions. These two subjects have not been treated systematically either in text books or in handbooks on stirred-tank mixing, although the results of both experimental and theoretical investigations have been reported on many occasions. Finally gas-inducing mechanically agitated systems are dealt with. The applications of this type of agitation system will become increasingly attractive from the standpoint of rationalization of stirred-tank operations as well as environmental protection.

This detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in major wheat production countries. The topics, addressed by internationally renowned scientists active in the field, cover methods underpinning the latest developments in the field of wheat biotechnology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Wheat Biotechnology: Methods and Protocols serves as a vital resource for scientists working to breed future high-yielding wheat varieties to sustain a growing population in an increasingly unpredictable world.

This volume deals with "Microbial Production of L-Amino Acids" and presents five comprehensive, expert and actual review articles on the modern production of Amino Acids by application of biotechnologically optimized microorganisms. This includes not only the modern techniques of enzyme, metabolic and transport engineering but also sophisticated analytical methods like metabolic flux analysis and subsequent pathway modeling. A general review about industrial processes of Amino Acid production provides a comprehensive overview about recent strain development as well as fermentation technologies. It was our special interest to focus the other articles on the most important and best selling amino acids on the world market i.e. L-Glutamate, L-Lysine and L-Threonine. The authors of this special volume have contributed significantly to the progress of Amino Acid biotechnology in the last decades and earn our special gratitude and admiration for their expert review articles.

Organoselenium Chemistry is a unique resource in this branch of organic/organometallic chemistry. The authors give an overview of synthesis strategies, introduce bioactive and environmentally friendly organoselenium compounds and discuss their applications from organic synthesis to the clinic.

For many years the use of chemical agents such as pesticides and herbicides has been effective in controlling the many varieties of pests that infest both agricultural crops and backyard gardens. However, these pests are gradually becoming resistant to these agents, because the agents themselves are acting as selective factors making the pests better and better able to resist and persist. As a result, the use of biological controlling agents is increasing.
This book is a comprehensive and authoritative handbook of biological control.
Key Features
* Introduction (preface plus 2 chapters)
* Principles and processes (12 chapters)
* Agents, biology, and methods (6 chapters)
* Applications (10 chapters)
* Research (2 chapters)

There is hardly any technical library in the world in which the volumes of the Chemical Formulary (Volumes 1-34) do not occupy a prominent place. Chemists both experienced and beginner, continually refer to them. Volume 33 counts numerous experts among it's contributors, such as Editor-in-chief H. Bennett. It does not duplicate any of the formulas included in previous volumes, but lists a wide array of modern and salable products from all branches of the chemical industries. An excellent reference for formulation problems.

The objective of the Springer Handbook of Enzymes is to provide in concise form data on enzymes that have been sufficiently well characterized. Data sheets are arranged in their EC-Number sequence. Usually each volume comprises one enzyme class, although sometimes the enzyme classes have to be divided into several volumes. Considerable progress has been made in enzymology since the publication of the first edition (published as "Enzyme Handbook"), and now many enzymes are newly classified or reclassified. In the 2nd edition each entry is correlated with references and one or more source organisms. New datafields are created, e.g., "application" and "engineering" (for the properties of enzymes where the sequence has been changed). Altogether the amount of data has doubled so that the 2nd edition will consist of approximately 25 volumes. This collection is an indispensable source of information for biochemists, biotechologists, organic and analytical chemists, and food scientist.

My journey into this fascinating field of biotechnology started about 26 years ago at a small biotechnology company in South San Francisco called Genentech. I was very fortunate to work for the company that begat the biotech industry during its formative years. This experience established a solid foundation from which I could grow in both the science and business of biotechnology. After my fourth year of working on Oyster Point Boulevard, a close friend and colleague left Genentech to join a start-up biotechnology company. Later, he approached me to leave and join him in of all places - Oklahoma. He persisted for at least a year before I seriously considered his proposal. After listening to their plans, the opportunity suddenly became more and more intriguing. Finally, I took the plunge and joined this ent- preneurial team in cofounding and growing a start-up biotechnology company. Making that fateful decision to leave the security of a larger company was extremely difficult, but it turned out to be the beginning of an entrepreneurial career that forever changed how I viewed the biotechnology industry. Since that time, I have been fortunate to have cofounded two other biotechnology com- nies and even participated in taking one of them public. During my career in these start-ups, I held a variety of positions, from directing the science, operations, regulatory, and marketing components, to subsequently becoming CEO.

The scope of opportunities in chemical and biomolecular engineering has grown tremendously in recent years. Careers in Chemical and Biomolecular Engineering conveys the breadth and depth of today's chemical and biomolecular engineering practice, and describes the intellectually enriching, socially conscious and financially lucrative opportunities available for such graduates in an ever-widening array of industries and applications. This book aims to help students interested in studying chemical engineering and biomolecular engineering to understand the many potential career pathways that are available in these dynamic fields - and is an indispensable resource for the parents, teachers, advisors and guidance counselors who support them, In addition to 10 chapters that discuss the roles such graduates play in many diverse industries, this book also features 25 Profile articles that share in-depth, first-person insight from industry-leading chemical and biomolecular engineers. These technical professionals discuss their work and educational experiences (in terms of both triumphs and challenges), and share wisdom and recommendations for students pursuing these two dynamic engineering disciplines.

The increasing demand for environmentally friendly materials and the need for cheaper fibres points the search in the direction of natural products such as bark, leaves, scales or shells. The aim of this book is to provide a forum to review the recent advances in the area of plant and animal-based composites and identify possible trends for further developments.

Tingyue Gu's second edition provides a comprehensive set of nonlinear multicomponent liquid chromatography (LC) models for various forms of LC, such as adsorption, size exclusion, ion-exchange, reversed-phase, affinity, isocratic/gradient elution and axial/radial flow LC. Much has advanced since the first edition of this book and the author's software, described here, is now used for teaching and research in 32 different countries. This book comes together with a complete software package with graphical user interface for personal computers, offered free for academic applications. Additionally, this book provides detailed methods for parameter estimation of mass transfer coefficients, bed voidage, particle porosity and isotherms. The author gives examples of how to use the software for predicitons and scale-up. In contrast to the first edition, authors do not need to deal with complicated math. Instead, they focus on how to obtain a few parameters for simulation and how to compare simulation results with experimental data. After reading the detailed descriptions in the book, a reader is able to use the simulation software to investigate chromatographic behavior without doing actual experiments. This book is aimed at readers who are interested in learning about LC behaviors and at those who want to scale up LC for preparative- and large-scale applications. Both academic personnel and industrial practitioners can benefit from the use of the book. This new edition includes: - New models and software for pellicular (cored) beads in liquid chromatography - Introduction of user-friendly software (with graphical user interface) - Detailed descriptions on how to use the software - Step-by-step instructions on parameter estimation for the models - New mass-transfer correlations for parameter estimation - Experimental methods for parameter estimation - Several actual examples using the model for product development and scale-up - Updated literature review

Biohydrogen is considered the most promising energy carrier and its utilization for energy storage is a timely technology. This book presents latest research results and strategies evolving from an international research cooperation, discussing the current status of Biohydrogen research and picturing future trends and applications.

C. Tollnick, G. Seidel, M. Beyer, K. Schugerl: Inverstigations of the Production of Cephalosporin C by Acremonium chrysogenum.- K. Friehs: Plasmid Copy Number and Plasmid Stability.- P.S.J. Cheetham: Bioprocesses for the Manufacture of Ingredients for Foods and Cosmetics.- I. Roy, S. Sharma, M.N.

"Cardiac Tissue Engineering: Methods and Protocols "presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, "Cardiac Tissue Engineering: Methods and Protocols "highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.

The title "Nano Biotechnology for Biomedical and Diagnostics Research" will address research aspects related to nanomaterial in imaging and biological research, nanomaterials as a biosensing tool, DNA nanotechnology, nanomaterials for drug delivery, medicinal and therapeutic application and cytotoxicity of nanomaterials. These topics will be covered by 16 different manuscripts. Amongst the authors that will contribute to the book are major scientific leaders such as S. Weiss - UCLA, I. Willner, and G. Golomb -- HUJI, S. Esener - UCSD, E.C. Simmel - Tech. Univ. Munchen, I. Medintz -- NRL, N. Hildebrandt - Universit Paris and more. The manuscripts in the book intend to present specifically biological, diagnostics and medical problems with their potential solution by nano technology or materials. In this respect this book is unique, since it would arise from the biological problems to the nano technology possible solution and not vice versa.