In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical, Cellular and Subcellular Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of applying nanotechnology to all areas of biomedical sciences.

Volume 17 of this highly acclaimed series features discussions on a new method of mapping and manipulating DNA with the use of RecA protein in combination with restriction enzymes, RNA pseudoknots, and platelet-derived growth factors and their receptors in cell differentiation and cell cycle control.

For physicians, surgeons, and scientists working on cardiovascular disorders, Applications of Biotechnology in Cardiovascular Therapeutics serves as an invaluable reference by collecting the essential writings of Dr. Kewal K. Jain on the topics of biotechnology as they relate to cardiovascular disease. This thorough volume includes such subjects as biotechnology and therapeutic delivery to the cardiovascular system, cell-selective targeted drug delivery, cell and gene therapies, including antisense and RNA interference, cutting-edge gene therapy approaches, as well as personalized cardiology as a way of integrating new technologies into the selection of the best possible treatment for an individual patient. Selected references from recent literature are collected for each chapter, and the text is supplemented by a variety of useful tables and figures. Comprehensive and up-to-date, Applications of Biotechnology in Cardiovascular Therapeutics will be tremendously useful for those working in life sciences and the pharmaceutical sciences, and the inclusion of some basics of cardiovascular diseases will greatly benefit nonmedical readers as well.

Following the routinization of assisted reproduction in the industrialized world, technologies such as in vitro fertilization, preimplantation genetic diagnosis, and DNA-based paternity testing have traveled globally and are now being offered to couples in numerous non-Western countries. This volume explores the application and impact of these advanced reproductive and genetic technologies in societies across the globe. By highlighting both the cross-cultural similarities and diverse meanings that technologies may assume as they enter multiple contexts, the book aims to foster understanding of both the technologies and the settings. Enhanced by cross-cultural perspectives, the book addresses the challenges that globalization presents to local understandings of science, technology, and medicine.

Providing overview, depth, and expertise, Essentials of Functional Foods is the key resource for all involved in the exciting and rapidly growing arena of functional foods. Every important aspect of functional foods and ingredients is covered, from technology, product groups, and nutrition, to safety, efficacy, and regulation. The editors and their expert contributors emphasize broadly based principles that apply to many functional foods. This book is essential reading for food scientists, researchers, and professionals who are developing, researching, or working with functional foods and ingredients in the food, drug, and dietary supplement industry.

Murray's new handbook on Gene Transfer and Expression Protocols sets forth both current and new methodologies in a clear, concise, easy-to-follow manner, following the successful formula of the classic volumes in Humana's Methods in Molecular Biology series. Each chapter is devoted to a thorough exposition of a single technique. An Introduction explains the significance of the protocol and provides background information. A Materials section lists all the requirements for the technique discussed. A Methods section details the procedure in a step-by-step protocol. A Notes section alerts the reader to pitfalls that may be encountered, as well as alternatives that may be used for successful completion of the experiment. Each technique is designed to guarantee optimum results.
This volume is an outstanding new benchtop manual that provides protocols for introducing an isolated gene into a cell line, using various transfection techniques. Topics and techniques include:
viral vectors a [ reporter genes a [ analysis of steady-state level of transcription a [ assay for newly initiated transcriptional complexes a [ immunocytological techniques a [ assay of structure and replication state of transfected genes a [ overall strategy for defining regulatory sequences
Providing the latest techniques in a fast-paced area, Gene Transfer and Expression Protocols is an absolutely essential handbook for everyone involved in cytogenetics, cell genetics, molecular biology, and related fields.

The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized - and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.

Bioinformatics is an integrative field of computer science, genetics, genomics, proteomics, and statistics, which has undoubtedly revolutionized the study of biology and medicine in past decades. It mainly assists in modeling, predicting and interpreting large multidimensional biological data by utilizing advanced computational methods. Despite its enormous potential, bioinformatics is not widely integrated into the academic curriculum as most life science students and researchers are still not equipped with the necessary knowledge to take advantage of this powerful tool. Hence, the primary purpose of our book is to supplement this unmet need by providing an easily accessible platform for students and researchers starting their career in life sciences. This book aims to avoid sophisticated computational algorithms and programming. Instead, it will mostly focus on simple DIY analysis and interpretation of biological data with personal computers. Our belief is that once the beginners acquire these basic skillsets, they will be able to handle most of the bioinformatics tools for their research work and to better understand their experimental outcomes. The third volume is titled In Silico Life Sciences: Agriculture. It focuses on plant genetic, genomic, transcriptomic, proteomic and metabolomics data. Using examples of new crop diseases-emergence, crop productivity and biotic/abiotic stress tolerance, this book illustrates how bioinformatics can be an integral components of modern day plant science research.

Collaboration plays an important role in the early development of com- nies. Among others, they provide opportunities to combine complementary resources, develop additional competencies, and generate valuable signals for investors. They are particularly important for biotechnology firms, whose resource base often is not sufficient to realize the market potential of their R&D findings. Strategic alliances thus are an integral part of the business model of most biotechnology companies, but their economic re- vance is not yet fully understood, since research has thus far neglected most industry-specific drivers of alliance value. Based on an event study, Hady Farag analyzes the capital-market re- tion to alliance-related news announcements and assesses their complex - fects on company value. In this regard, the present work represents the first comprehensive study of European biotechnology alliances. In addition to this unique database, the research approach and techniques in sample - lection, econometric and cross-sectional analyses are state-of-the-art. The author develops and empirically tests an integrative dynamic model of collaborative value drivers. These reflect the specific characteristics of biotechnology firms and biotechnology alliances. Moreover, the work - tends to so far entirely unresearched dynamic aspects of alliances, such as the value of contractual flexibilities, the impact of environmental unc- tainty, and the evolution of alliances over time. Overall, Hady Farag's work underscores the need to consider pluralistic influences on the value of collaborative ventures.

Controlled radical polymerization techniques for molecular imprinting, by Mark E. Byrne From bulk polymers to nanoparticles, by Lei Ye Post-imprinting and in-cavity functionalization, by Toshifumi Takeuchi Characterization of MIPs (affinity, selectivity, site heterogeneity...), by Richard Ansell Theoretical aspects and computer modelling, by Ian Nicholls MIPs in aqueous environments, by Bin Lu MIPs for binding macromolecules, by Kenneth J. Shea Solid phase extraction, by Ecevit Yilmaz Sensors, by Sergey A. Piletsky MIPs for catalysis and synthesis, by Marina Resmini Wastewater treatment, by Bo Mattiasson MIPs as tools for bioassays, biotransformation and drug delivery, by Meiping Zhao

We are in a phase of the evolution of biotechnology in which the true and potential commercial importance of carbohydrates is becoming appre- ated more fully. Progress in providing hard facts to establish the commercial value ofpolysaccharides and oligosaccharides is limited, as always, by lack of funding and by a relative shortage of skilled practitioners in the production and analysis of those materials. Carbohydrate science has a reputation, not unmerited, for technical difficulty owing to the structural similarity of the many monosaccharide monomers and the potential, and real, complexity of oligosaccharides and polysaccharides, particularly heterosaccharides conta- ing many different monomers. Modem analytical and synthetic methods, in many cases using enzyme technology, are beginning to allow this complexity to be unraveled. Carbohydrate Biotechnology Protocols is aimed at those newcomers who have an interest in the production and use of carbohydrate materials, but have shied away from involvement for lack of detailed descriptions of appropriate methods, including the type of practical hints that may be provided by those skilled in those methods, but that are rarely described in research papers. The majority of the contributions to this book conform to the established format of the Methods in Biotechnology series. They begin with the theoretical and c- mercial background to the method or group of methods, provide a list of the reagents and equipment required for the procedure, then give a detailed st- by-step description of how to carry out the protocol.

Since 9/11 and the U.S. anthrax attacks, public, and policy concerns about the security threats posed by biological weapons has increased significantly. As a result, there are now active international deliberations about what restrictions should be placed on the openness of scientific research. "Biotechnology, Security and the Search for Limits" examines these security implications for life science research as well as the methodological issues associated with conducting social research. In doing so the book considers the place of biological and social research in creating and responding to societal problems through drawing on diverse academic traditions such as discourse analysis, social problems studies, philosophy, action research, science and technology studies, politics, and public policy.

In Human Cloning a panel of distinguished philosophers, medical ethicists, religious thinkers, and social critics tackle the thorny problems raised by the now real possibility of human cloning. In their wide ranging reviews, the distinguished contributors critically examine the major arguments for and against human cloning, probe the implications of such a procedure for society, and critically evaluate the "Report and Recommendations of the National Bioethics Advisory Commission." The debate includes both religious and secular arguments, as well as an outline of the history of the cloning debate and a discussion of human cloning's impact on our sense of self and our beliefs about the meaning of life.

After the 1986 and 1989 volumes, this is the third volume on biotechnology for propagation of trees. Comprising 28 chapters contributed by international experts the book deals with fruit, ornamental, and forest trees, such as Black cherry, Sour cherry, Pomegranate, Loquat, "Ficus," Yellow poplar, Horse chestnut, Judas tree, Linden tree, Saskatoons, Taiwan sassafras, Plane-tree, Rattans, Bamboos, Engelmann spruce, White spruce, Larches, Hinoki cypress, Western redcedar, and various types of pines, i.e. Jack, Carribean, Eldarica, Slash, Egg-cone, Maritime, Ponderosa, Eastern white, Loblolly pine. Trees III is an excellent reference book for scientists, educators, and students of forestry, botany, genetics, and horticulture, who are interested in tree biotechnology.

The book provides a fascinating overview about current and sophisticated developments in applied entomology that are powered by molecular biology and that can be summarized under a novel term: insect biotechnology. By analogy with the application of powerful molecular biological tools in medicine (red biotechnology), plant protection (green biotechnology) and industrial processing (white biotechnology), insect biotechnology (yellow biotechnology) provides novel tools and strategies for human welfare and nutrition. Insect Biotechnology has emerged as a prospering discipline with considerable economic potential, and encompasses the use of insect model organisms and insect-derived molecules in medical research as well as in modern plant protection measures.

Biosensors have captured the imagination of the world's scientific and commercial communities by combining interdisciplinary skills of biologists, physicists, chemists and engineers to provide innovative solutions to analytical problems. Biosensors are applicable to clinical diagnostics, food analysis, cell culture monitoring, environmental control and various military situations. Ever increasing demands for rapid and convenient analyses of a wide variety of materials in diverse locations has led to intense interest in the fusion of biology and electronics which mimics our principal concern: the effect of materials and environments on living systems. This series, "Advances in Biosensors", presents a compendium of research papers, in which authorities in the field of biosensors provide an up-to-date overview of their laboratory's contribution, summarizing the primary research as it has appeared, possibly scattered, in the journal and conference literature, and reflecting on their findings. The net result will be intense, yet highly readable accounts of the state of the art at this leading edge of analytical technology.

This book provides in-depth insights into the regulatory frameworks of five countries and the EU concerning the regulation of genome edited plants. The country reports form the basis for a comparative analysis of the various national regulations governing genetically modified organisms (GMOs) in general and genome edited plants in particular, as well as the underlying regulatory approaches.The reports, which focus on the regulatory status quo of genome edited plants in Argentina, Australia, Canada, the EU, Japan and the USA, were written by distinguished experts following a uniform structure. On this basis, the legal frameworks are compared in order to foster a rational assessment of which approaches could be drawn upon to adjust, or to completely realign, the current EU regime for GMOs. In addition, a separate chapter identifies potential best practices for the regulation of plants derived from genome editing.

For courses in biotechnology. Introduction to Biotechnology brings the latest information students need to understand the science and business of biotechnology. The popular text emphasizes the future of biotechnology and the biotechnology student's role in that future with balanced coverage of basic cell and molecular biology, fundamental techniques, historical accounts, new advances, and hands-on applications. The 4th Edition features content updates in every chapter that reflect the most relevant, up-to-date changes in technology, applications, ethical issues, and regulations. Additionally, every chapter now includes an analytic Case Study that highlights current research and asks students to use what they've learned about key chapter concepts to answer questions. New Career Profiles, written by biotech professionals and available on the Companion Website along with additional career resources, highlight potential jobs in the biotech industry. The chapter on biotechnology regulations has been revised to include regulations involving international bodies.

Plant secondary metabolism is an economically important source of fine chemicals, such as drugs, insecticides, dyes, flavours, and fragrances. Moreover, important traits of plants such as taste, flavour, smell, colour, or resistance against pests and diseases are also related to secondary metabolites. The genetic modification of plants is feasible nowadays. What does the possibility of engineering plant secondary metabolite pathways mean? In this book, firstly a general introduction is given on plant secondary metabolism, followed by an overview of the possible approaches that could be used to alter secondary metabolite pathways. In a series of chapters from various authorities in the field, an overview is given of the state of the art for important groups of secondary metabolites. No books have been published on this topic so far. This book will thus be a unique source of information for all those involved with plants as chemical factories of fine chemicals and those involved with the quality of food and ornamental plants. It will be useful in teaching graduate courses in the field of metabolic engineering in plants.

What should the average person know about science? Because science is so central to life in the 21st century, science educators and other leaders of the scientific community believe that it is essential that everyone understand the basic concepts of the most vital and far-reaching disciplines. Biotechnology 101 does exactly that. This accessible volume provides readers - whether students new to the field or just interested members of the lay public - with the essential ideas of biotechnology using a minimum of jargon and mathematics. Concepts are introduced in a progressive order so that more complicated ideas build on simpler ones, and each is discussed in small, bite-sized segments so that they can be more easily understood. Biotechnology 101 provides an introduction to all the areas of biotechnology, covering such topics as: BLA History of the Science Behind Biotechnology BLThe Tools of Biotechnology BLBiotechnology Innovations BLPrincipal people of Biotechnology BLBiotechnology in Everyday Life BLEthical Issues of Biotechnology This short volume will enable students and lay people to understand the basics of one of the most important scientific fields of endeavor for the future.

The book provides background knowledge on the molecular biology of plants and a comprehensive description of plant biotechnology. It is in three parts. Part A deals with an overview of Plant Molecular Biology. Part B is concerned with Plant Tissue Culture. Part C is about Plant Genetic Engineering and Biotechnological Applications. Several transgenic applications dealt with in detail are in areas of engineering plants for stress tolerance, metabolic engineering of plants, transplastomic technology, crop protection, and antisense technology in plants. One of the highlights of this book is that it presents the concepts of both plant molecular biology and plant biotechnology.

Many industrial, power generation and chemical processes produce unwanted fine particulate material as a consequence of their operation. Electrostatic precipitation is a highly efficient method of removing entrained particulate contaminants from exhaust gases and is extensively used in these industries to limit particulate emissions. New legislation aimed at improving the environment by further limiting these discharges has resulted in the technique undergoing considerable development over the past decade, to the point where it has become the method of choice, over a wide range of applications, for limiting particulate discharges. In this new book, the editor has brought together an international team of contributors, mainly industrialists and consultants, to produce an authorative and practical guide to electrostatic precipitation. This book is of interest to all those in process industries or power generation and to academics concerned with gas cleaning and environmental issues.

The medical device and drug industries standards in analytical methodology and are consistently among the strongest techno- quality control. logical performers. Materials are a key The users of Biomaterials Engineering ingredient in their dynamic growth. Devel- and Devices: Human Applications will r- opment of these materials is in a constant resent a broad base of backgrounds ranging state of activity, with the challenge of re- from the basic sciences (e. g. , polymer placing old materials that cannot withstand chemistry and biochemistry) to more the tests of time, and the new materials' applied disciplines (e. g. , mechanical/ needs coming to the forefront in modern chemical engineering, orthopedics, and applications. This new reference text, pharmaceutics). To meet varied needs, each Biomaterials Engineering and Devices: chapter provides clear ancd fully detailed Human Applications, focuses on materials discussions. This in-depth, but practical, used in or on the human body-materials coverage should also assist recent indu- that define the world of "biomaterials. " ees to the biomaterials circle. The editors Biomaterials Engineering and Devices: trust that this reference textbook conveys Human Applications focuses on mate- the intensity of this fast moving field in an rials development and characterization. enthusastic presentation. Chapters deal with issues in the selection of Donald L. Wise, PHD proper biomaterials from biocompatibility Debra J. Trantolo, PHD to biostability to structure/function relation- Kai-Uwe Lewandrowski, MD ships. Chapters also focus on the use of Joseph D. Gresser, PHD specific biomaterials based on their physio- Mario V.