This Volume presents protocols for systems and synthetic biology applications in the field of hydrocarbon and lipid microbiology. It complements another Volume that describes generic protocols for wet experimental and computer-based systems and synthetic biology studies. The protocols in this Volume demonstrate how to employ systems and synthetic biology approaches in the design of microbes for the production of esters, isoprenoids, hydrophobic polymers, rhamnolipid biosurfactant, and peptide antimicrobial and thioether-stabilised molecules. Also presented is a protocol for the engineering of transcription factor-based biosensors for intracellular products, and another for the creation of a synthetic hydroxylase with novel activity for the selective oxyfunctionalisation of linear alkanes. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Biopolymers from Renewable Resources is a compilation of information on the diverse and useful polymers derived from agricultural, animal, and microbial sources. The volume provides insight into the diversity of polymers obtained directly from, or derived from, renewable resources. The beneficial aspects of utilizing polymers from renewable resources, when considering synthesis, pro cessing, disposal, biodegradability, and overall material life-cycle issues, suggests that this will continue to be an important and growing area of interest. The individual chapters provide information on synthesis, processing and properties for a variety of polyamides, polysaccharides, polyesters and polyphenols. The reader will have a single volume that provides a resource from which to gain initial insights into this diverse field and from which key references and contacts can be drawn. Aspects of biology, biotechnology, polymer synthesis, polymer processing and engineering, mechanical properties and biophysics are addressed to varying degrees for the specific biopolymers. The volume can be used as a reference book or as a teaching text. At the more practical level, the range of important materials derived from renewable resources is both extensive and impressive. Gels, additives, fibers, coatings and films are generated from a variety of the biopolymers reviewed in this volume. These polymers are used in commodity materials in our everyday lives, as well as in specialty products."

The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized - and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.

Biosensors offer clear and distinct advantages over standard analytical methods for the direct monitoring of environmental pollutants in the field, such as real-time detection with minimum sample preparation and handling. The present book highlights recent advantages that will be of great value to a range of scientists, researchers and students dealing with analytical and environmental chemistry and biosensor technology. It presents recent trends in analytical methodology for the determination of indoor and outdoor pollutants, advances in DNA, biological and recognition-based sensors, examples of biosensors for use in field and water analysis, biosensors based on non-aqueous systems, and recent advances in the miniaturisation and micromachining of biosensors.

CONTENTS - 1. INTRODUCTION - 2. ANIMAL FATS AND OILS - 3. VEGETABLE FATS AND OILS - 4. FATTY ACIDS-FATTY ALCOHOLS-GLYCEROL - 5. SURFACTANTS AND SURFACE ACTIVITY - 6. PRODUCTION OF FATS AND OILS - 7. SOAP MANUFACTURE - 8. SYNTHETIC DETERGENTS - 9. ANALYSIS OF OILS AND DETERGENTS - INDEX - Preface - Presented in this book are quantities of data, gathered during many years in my practice of chemistry, either as resident chemist in manufacturing plants, or as a consultant to the soap and related industries. The style is didactic and such that it may readily be understood by non-technical personnel. Involved theoretical and technical discussions have been avoided. As far as possible, references have been included at the end of each chapter. Although much of the information contained in this work is well within my own experience, much of it has been gleaned also from manufacturers' technical bulletins or through private correspondence. Many patents have been cited, merely as examples of products discussed; this is not to be construed as recommending the infringement of any such patents. The various formulations are presented in good faith, but no warranty is given, nor is freedom from any patent to be inferred. With a little ingenuity on the part of the reader, many useful and efficient products may be made along the lines discussed, without in any way infringing on patented products. Because of numerous factors affecting results, all the formulations cited herein, although believed to be reliable (most of them having emanated from the greatest laboratories in the world) should be tested thoroughly. It is especially recommended that manufacturers make their own tests to determine the suitability of the formulations, for the specific applications intended. I am indebted to the manufacturers of the many chemicals named in the book, for information which I gathered either from direct correspondence or from their technical bulletins; also for their many samples with which I compounded some of the formulas. Special mention should be made of those companies that supplied photographs of equipment and installations depicting various processes. I have tried to include all information pertinent to each formula, and credit has been given to all identified suppliers, so that additional information, if needed, may be obtained from the manufacturers of the basic materials used. Finally, I am grateful to the publishers for valuable assistance in the organization and presentation of my quantities of material which now comes out as not merely a second edition of my Oil, Fat, and Soap, but as a much more comprehensive new book.|CONTENTS - 1. AEROSOLS - 2. BACTERICIDES-FUNGICIDES - SANITIZERS - 3. CLEANERS FOR HOUSEHOLD AND INDUSTRY - 4. CARE OF FLOORS - 5. LAUNDERING AND DRY CLEANING - 6. TREATMENT OF METALS - 7. PESTICIDES - 8. MISCELLANEOUS - BIBLIOGRAPHY - APPENDIX - Glossary - Suppliers of Materials and Equipment - Useful Tables - Patents - Periodicals of Interest - Associations Related to the Industry - INDEX -

It is our pleasure to present the 53rd volume of the Biotechnology in Agric- ture and Forestry (BAF) series. This is the second issue of the BAF series edited by the new editorial team consisting of Professors Horst Lorz, University of Hamburg, Hamburg, Germany, Jack Widholm, University of Illinois, Urbana, USA,and Toshiyuki Nagata,University of Tokyo,Tokyo,Japan. This series was originally founded by the late Professor Y. P. S. Bajaj,Delhi,India,in 1986. The current volume is somewhat unique, since in this volume only one plant cell line,the tobacco BY-2 cell line,is handled,while previous volumes mainly dealt with plants having certain economical importance. Nonetheless, the three editors of this volume,Professors Dirk Inze,Seiichiro Hasezawa and Toshiyuki Nagata,believe that most scientists who are working in the ?eld of plant s- ences will enjoy seeing this volume as a kind of source book of the unique tobacco BY-2 cells. Indeed,tobacco BY-2 cells have,over the years,gained the status as a model plant system,comparable to HeLa cells for human research. The current book is very timely because a wealth of basic knowledge on plant cells related to,e. g. ,cell division,cytoskeleton,cytokinesis,plant hormone s- naling,etc. ,has been gathered from experiments with this cell line. As re?ected in the contents,the accumulated knowledge of the BY-2 cell line is enormous and there is no other cell line that has been so important for progress in the plant sciences. Such knowledge should be shared with scientists from the ?elds of both applied and basic plant science.

In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical, Cellular and Subcellular Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of applying nanotechnology to all areas of biomedical sciences.

Volume 17 of this highly acclaimed series features discussions on a new method of mapping and manipulating DNA with the use of RecA protein in combination with restriction enzymes, RNA pseudoknots, and platelet-derived growth factors and their receptors in cell differentiation and cell cycle control.

Following the routinization of assisted reproduction in the industrialized world, technologies such as in vitro fertilization, preimplantation genetic diagnosis, and DNA-based paternity testing have traveled globally and are now being offered to couples in numerous non-Western countries. This volume explores the application and impact of these advanced reproductive and genetic technologies in societies across the globe. By highlighting both the cross-cultural similarities and diverse meanings that technologies may assume as they enter multiple contexts, the book aims to foster understanding of both the technologies and the settings. Enhanced by cross-cultural perspectives, the book addresses the challenges that globalization presents to local understandings of science, technology, and medicine.

In Human Cloning a panel of distinguished philosophers, medical ethicists, religious thinkers, and social critics tackle the thorny problems raised by the now real possibility of human cloning. In their wide ranging reviews, the distinguished contributors critically examine the major arguments for and against human cloning, probe the implications of such a procedure for society, and critically evaluate the "Report and Recommendations of the National Bioethics Advisory Commission." The debate includes both religious and secular arguments, as well as an outline of the history of the cloning debate and a discussion of human cloning's impact on our sense of self and our beliefs about the meaning of life.

Murray's new handbook on Gene Transfer and Expression Protocols sets forth both current and new methodologies in a clear, concise, easy-to-follow manner, following the successful formula of the classic volumes in Humana's Methods in Molecular Biology series. Each chapter is devoted to a thorough exposition of a single technique. An Introduction explains the significance of the protocol and provides background information. A Materials section lists all the requirements for the technique discussed. A Methods section details the procedure in a step-by-step protocol. A Notes section alerts the reader to pitfalls that may be encountered, as well as alternatives that may be used for successful completion of the experiment. Each technique is designed to guarantee optimum results.
This volume is an outstanding new benchtop manual that provides protocols for introducing an isolated gene into a cell line, using various transfection techniques. Topics and techniques include:
viral vectors a [ reporter genes a [ analysis of steady-state level of transcription a [ assay for newly initiated transcriptional complexes a [ immunocytological techniques a [ assay of structure and replication state of transfected genes a [ overall strategy for defining regulatory sequences
Providing the latest techniques in a fast-paced area, Gene Transfer and Expression Protocols is an absolutely essential handbook for everyone involved in cytogenetics, cell genetics, molecular biology, and related fields.

We are in a phase of the evolution of biotechnology in which the true and potential commercial importance of carbohydrates is becoming appre- ated more fully. Progress in providing hard facts to establish the commercial value ofpolysaccharides and oligosaccharides is limited, as always, by lack of funding and by a relative shortage of skilled practitioners in the production and analysis of those materials. Carbohydrate science has a reputation, not unmerited, for technical difficulty owing to the structural similarity of the many monosaccharide monomers and the potential, and real, complexity of oligosaccharides and polysaccharides, particularly heterosaccharides conta- ing many different monomers. Modem analytical and synthetic methods, in many cases using enzyme technology, are beginning to allow this complexity to be unraveled. Carbohydrate Biotechnology Protocols is aimed at those newcomers who have an interest in the production and use of carbohydrate materials, but have shied away from involvement for lack of detailed descriptions of appropriate methods, including the type of practical hints that may be provided by those skilled in those methods, but that are rarely described in research papers. The majority of the contributions to this book conform to the established format of the Methods in Biotechnology series. They begin with the theoretical and c- mercial background to the method or group of methods, provide a list of the reagents and equipment required for the procedure, then give a detailed st- by-step description of how to carry out the protocol.

Controlled radical polymerization techniques for molecular imprinting, by Mark E. Byrne From bulk polymers to nanoparticles, by Lei Ye Post-imprinting and in-cavity functionalization, by Toshifumi Takeuchi Characterization of MIPs (affinity, selectivity, site heterogeneity...), by Richard Ansell Theoretical aspects and computer modelling, by Ian Nicholls MIPs in aqueous environments, by Bin Lu MIPs for binding macromolecules, by Kenneth J. Shea Solid phase extraction, by Ecevit Yilmaz Sensors, by Sergey A. Piletsky MIPs for catalysis and synthesis, by Marina Resmini Wastewater treatment, by Bo Mattiasson MIPs as tools for bioassays, biotransformation and drug delivery, by Meiping Zhao

This book focuses on recent advances and future trends in the methods and applications of technologies that are used in neuroscience for the evaluation, diagnosis and treatment of neurological diseases and conditions or for the improvement of quality of life. The editors have assembled contributions from a range of international experts, to bring together key topics in neurotechnology, neuroengineering, and neurorehabilitation. The book explores biomedical signal processing, neuroimaging acquisition and analysis, computational intelligence, virtual and augmented reality, biometrics, machine learning and neurorobotics, human machine interaction, mobile apps and discusses ways in which these neural technologies can be used as diagnostic tools, research methods, treatment modalities, as well as in devices and apps in everyday life. This cross-disciplinary topic is of particular interest to researchers and professionals with a background in neuroscience-related disciplines and neurotechnology, but also touches on a wide range of other fields including biomedical engineering, AI, medicine, healthcare, security and industry, among others.

Collaboration plays an important role in the early development of com- nies. Among others, they provide opportunities to combine complementary resources, develop additional competencies, and generate valuable signals for investors. They are particularly important for biotechnology firms, whose resource base often is not sufficient to realize the market potential of their R&D findings. Strategic alliances thus are an integral part of the business model of most biotechnology companies, but their economic re- vance is not yet fully understood, since research has thus far neglected most industry-specific drivers of alliance value. Based on an event study, Hady Farag analyzes the capital-market re- tion to alliance-related news announcements and assesses their complex - fects on company value. In this regard, the present work represents the first comprehensive study of European biotechnology alliances. In addition to this unique database, the research approach and techniques in sample - lection, econometric and cross-sectional analyses are state-of-the-art. The author develops and empirically tests an integrative dynamic model of collaborative value drivers. These reflect the specific characteristics of biotechnology firms and biotechnology alliances. Moreover, the work - tends to so far entirely unresearched dynamic aspects of alliances, such as the value of contractual flexibilities, the impact of environmental unc- tainty, and the evolution of alliances over time. Overall, Hady Farag's work underscores the need to consider pluralistic influences on the value of collaborative ventures.

Since 9/11 and the U.S. anthrax attacks, public, and policy concerns about the security threats posed by biological weapons has increased significantly. As a result, there are now active international deliberations about what restrictions should be placed on the openness of scientific research. "Biotechnology, Security and the Search for Limits" examines these security implications for life science research as well as the methodological issues associated with conducting social research. In doing so the book considers the place of biological and social research in creating and responding to societal problems through drawing on diverse academic traditions such as discourse analysis, social problems studies, philosophy, action research, science and technology studies, politics, and public policy.

Providing overview, depth, and expertise, Essentials of Functional Foods is the key resource for all involved in the exciting and rapidly growing arena of functional foods. Every important aspect of functional foods and ingredients is covered, from technology, product groups, and nutrition, to safety, efficacy, and regulation. The editors and their expert contributors emphasize broadly based principles that apply to many functional foods. This book is essential reading for food scientists, researchers, and professionals who are developing, researching, or working with functional foods and ingredients in the food, drug, and dietary supplement industry.

This book provides in-depth insights into the regulatory frameworks of five countries and the EU concerning the regulation of genome edited plants. The country reports form the basis for a comparative analysis of the various national regulations governing genetically modified organisms (GMOs) in general and genome edited plants in particular, as well as the underlying regulatory approaches.The reports, which focus on the regulatory status quo of genome edited plants in Argentina, Australia, Canada, the EU, Japan and the USA, were written by distinguished experts following a uniform structure. On this basis, the legal frameworks are compared in order to foster a rational assessment of which approaches could be drawn upon to adjust, or to completely realign, the current EU regime for GMOs. In addition, a separate chapter identifies potential best practices for the regulation of plants derived from genome editing.

Modern Luminescence From Fundamental Concepts to Materials and Applications, Volume Four: Luminescence in Solid State Devices is a multivolume work that reviews the fundamental principles, properties and applications of luminescent materials. Topics addressed include 1) The key concepts of luminescence with a focus on important characterization techniques to understand a wide category of luminescent materials, 2) The most relevant luminescent materials such as transition metals, rare-earth materials, actinide-based materials, and organic materials, and 3) The emerging applications of luminescent materials in biomedicine, solid state devices, and the development of hybrid materials. The book reviews the latest advances in luminescent materials and their application in solid state lighting, energy and space. The volume also discusses advanced applications such as photonic energy materials, anti-counterfeiting, high power applications, and more. It will be suitable for materials scientists and engineers in academia and industry R&D.

For physicians, surgeons, and scientists working on cardiovascular disorders, Applications of Biotechnology in Cardiovascular Therapeutics serves as an invaluable reference by collecting the essential writings of Dr. Kewal K. Jain on the topics of biotechnology as they relate to cardiovascular disease. This thorough volume includes such subjects as biotechnology and therapeutic delivery to the cardiovascular system, cell-selective targeted drug delivery, cell and gene therapies, including antisense and RNA interference, cutting-edge gene therapy approaches, as well as personalized cardiology as a way of integrating new technologies into the selection of the best possible treatment for an individual patient. Selected references from recent literature are collected for each chapter, and the text is supplemented by a variety of useful tables and figures. Comprehensive and up-to-date, Applications of Biotechnology in Cardiovascular Therapeutics will be tremendously useful for those working in life sciences and the pharmaceutical sciences, and the inclusion of some basics of cardiovascular diseases will greatly benefit nonmedical readers as well.

Plant secondary metabolism is an economically important source of fine chemicals, such as drugs, insecticides, dyes, flavours, and fragrances. Moreover, important traits of plants such as taste, flavour, smell, colour, or resistance against pests and diseases are also related to secondary metabolites. The genetic modification of plants is feasible nowadays. What does the possibility of engineering plant secondary metabolite pathways mean? In this book, firstly a general introduction is given on plant secondary metabolism, followed by an overview of the possible approaches that could be used to alter secondary metabolite pathways. In a series of chapters from various authorities in the field, an overview is given of the state of the art for important groups of secondary metabolites. No books have been published on this topic so far. This book will thus be a unique source of information for all those involved with plants as chemical factories of fine chemicals and those involved with the quality of food and ornamental plants. It will be useful in teaching graduate courses in the field of metabolic engineering in plants.

After the 1986 and 1989 volumes, this is the third volume on biotechnology for propagation of trees. Comprising 28 chapters contributed by international experts the book deals with fruit, ornamental, and forest trees, such as Black cherry, Sour cherry, Pomegranate, Loquat, "Ficus," Yellow poplar, Horse chestnut, Judas tree, Linden tree, Saskatoons, Taiwan sassafras, Plane-tree, Rattans, Bamboos, Engelmann spruce, White spruce, Larches, Hinoki cypress, Western redcedar, and various types of pines, i.e. Jack, Carribean, Eldarica, Slash, Egg-cone, Maritime, Ponderosa, Eastern white, Loblolly pine. Trees III is an excellent reference book for scientists, educators, and students of forestry, botany, genetics, and horticulture, who are interested in tree biotechnology.

The book provides background knowledge on the molecular biology of plants and a comprehensive description of plant biotechnology. It is in three parts. Part A deals with an overview of Plant Molecular Biology. Part B is concerned with Plant Tissue Culture. Part C is about Plant Genetic Engineering and Biotechnological Applications. Several transgenic applications dealt with in detail are in areas of engineering plants for stress tolerance, metabolic engineering of plants, transplastomic technology, crop protection, and antisense technology in plants. One of the highlights of this book is that it presents the concepts of both plant molecular biology and plant biotechnology.