The second part of an updated edition of the classic "Methods in Cell Biology, "Volume 48, this book emphasizes diverse methods and technologies needed to investigate "C. elegans," both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of "C. elegans" for years to come.
Diverse, up-to-date techniques covered will be useful to the broadening community of "C. elegans" researchers for years to comeChapters written by leaders in the fieldTried and true methods deliver busy researchers a one-stop compendium of essential protocols

Lipids are a broad group of naturally occurring molecules which includes fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides, phospholipids, and others. The main biological functions of lipids include energy storage, as structural components of cell membranes, and as important signaling molecules. This volume of Methods in Cell Biology covers such areas as Membrane structure and dynamics, Imaging, and Lipid Protein Interactions. It will be an essential tool for researchers and students alike.
Covers such areas as membrane structure and dynamics, imaging, and lipid protein interactionsAn essential tool for researchers and students alikeInternational authorsRenowned editors

Written by leading researchers, this text explores the intersection of genetics and metabolomics, and points the way to more comprehensive studies of inborn variation of metabolism. All chapters refer to one or more published experimental datasets.

First published in 1943, "Vitamins and Hormones" is the longest-running serial published by Academic Press. The Editorial Board now reflects expertise in the field of hormone action, vitamin action, X-ray crystal structure, physiology and enzyme mechanisms.

Under the capable and qualified editorial leadership of Dr. Gerald Litwack, "Vitamins and Hormones" continues to publish cutting-edge reviews of interest to endocrinologists, biochemists, nutritionists, pharmacologists, cell biologists and molecular biologists. Others interested in the structure and function of biologically active molecules like hormones and vitamins will, as always, turn to this series for comprehensive reviews by leading contributors to this and related disciplines.

This volume focuses on stem cell regulators.
Longest running series published by Academic Press Contributions by leading international authorities

In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondrial Regulation: Methods and Protocols seeks to aid advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry and bioenergetics.

The present book intends to provide an update on immunosenescence and how deficiencies in the immune system contribute to a higher susceptibility to infections, decline in organ function, reduced vaccination responses, age-related disease and the ageing process itself, negatively affecting longevity. Our focus is on the main changes in immune system cells and their products occurring during the ageing process and the possible consequences for health and disease. This includes: discussion of the modulatory and/or suppressive mechanisms associated with the alterations in T regulatory cells, B regulatory cells and Myeloid Derived Suppressor cells; changes in the immune system observed in chronic neurodegenerative diseases, cancer, lung disease and frailty will also be discussed. Most importantly we provide recent literature information about possible interventions (focusing on physical activity) that could alleviate the negative effects of immunosenescence. The Ageing Immune System and Health is a comprehensive guide on the field intended to all physicians, researchers, professors and students interested on relationship between immune system, ageing and health.

Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Necrosis: Methods and Protocols describes techniques in an easy to follow manner, with details so that beginners can succeed with challenging techniques.

This volume of "Current Topics in Membranes" focuses on adrenergic receptor biology, beginning with a review of past successes and historical perspectives then further discussing current general trends in adrenic receptor studies in various contexts. This publication also includes discussions of the role and relationship of adrenergic receptors to different systems and diseases, establishing adrenergic receptor biology as a needed, practical reference for researchers.

The present volume continues the trend established in previous volumes in this series on Advances in Structural Biology. As in the past, diverse topics of current importance relevant to the theme of the series are included in the fourth volume.

This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson's disease, especially young onset Parkinson's disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy.

This volume of "Methods in Cell Biology, " the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999.
* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* Illustrates many techiques in full-color
* Summarizes the Zebrafish Genome Project

First published in 1943, "Vitamins and Hormones" is the longest-running serial published by Academic Press. The Editorial Board now reflects expertise in the field of hormone action, vitamin action, X-ray crystal structure, physiology and enzyme mechanisms.

Under the capable and qualified editorial leadership of Dr. Gerald Litwack, "Vitamins and Hormones" continues to publish cutting-edge reviews of interest to endocrinologists, biochemists, nutritionists, pharmacologists, cell biologists and molecular biologists. Others interested in the structure and function of biologically active molecules like hormones and vitamins will, as always, turn to this series for comprehensive reviews by leading contributors to this and related disciplines.

This volume focuses on vitamins and the immune system.
*Longest running series published by Academic Press
*Contributions by leading international authorities

The cytoskeleton is comprised of a variety of specialized proteins, and is a dynamic structure that is involved in the majority of key cellular events. There is increasing interest in the role of the cytoskeleton in human disease. This volume brings together human disease states where cytoskeletal disruptions are driving disease. Our emerging understanding of the molecular and cellular events that drive cytoskeletal mediated diseases including cancer, heart disease, myopathies and skin disorders, are also helping shape targeted therapeutic approaches to treating these diseases.

Required reading for anyone involved in cell signaling research with articles written and edited by experts in the field. This title covers disease states such as lymphoid leukemia, breast cancer, pulmonary fibrosis, systemic sclerosis, andinflammatory bowel disease, along with up-to-date research on signaling systems and mutations in
transcription factors that provide new targets for treating disease.
Articles written and edited by experts in the field
Thematic volume covering disease states such as lymphoid leukemia, breast cancer, pulmonary fibrosis, systemic sclerosis, and inflammatory bowel disease
Up-to-date research on signaling systems and mutations in transcription factors that provide new targets for treating disease

This volume of Current Topics in Membranes focuses on Membrane Fusion, beginning with fusion and fission of lipid bilayers, with reviews focused on hemifusion and dynamic remodeling of membranes catalyzed by dynamin. Other topics discussed include viral fusion, intracellular fusion, developmental cell fusion, and theoretical modeling.

This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.

This book presents some of the most advanced experimental systems in which the role of forces has been dissected. It explores the physical principles accounting for how forces can affect soft matter such as our cells, and presents some of the methods used to measure or assess the role of forces.

Presents some of the most advanced experimental systems in which the role of forces has been dissected.
It explores the physical principles accounting for how forces can affect soft matter such as our cells, and presents some of the methods used to measure or assess the role of forces.
Appeals to both physicists eager to get familiar with biological systems, and to biologists curious about the physical principles behind what they observe.

Cell-cell adhesion is fundamental for the development and homeostasis of animal tissues and organs. Adherens junctions (AJs) are the best understood cell-cell adhesion complexes. In this volume, internationally recognized experts review AJ biology over a wide range of organization; from atoms to molecules, to protein complexes, molecular networks, cells, tissues, and overall animal development. AJs have also been an integral part of animal evolution, and play central roles in cancer development, pathogen infection and other diseases. This book addresses major questions encompassing AJ biology. - How did AJs evolve? - How do cadherins and catenins interact to assemble AJs and mediate adhesion? - How do AJs interface with other cellular machinery to couple adhesion with the whole cell? - How do AJs affect cell behaviour and multicellular development? - How can abnormal AJ activity lead to disease? Valuable for both newcomers and experts in the field, this book offers a comprehensive resource for the research laboratory and a teaching tool for advanced undergraduate and graduate courses in cell and developmental biology.

Cytosol, the liquid found inside cells, is the site for multiple cell processes, including signaling from the cell membrane to sites within the cell. Cytosolic signaling mechanisms are researched and studied in graduate programs in cell biology, molecular biology, biochemistry, pharmacology, molecular and cellular physiology, pharmacy, and biomedical sciences.

Articles written and edited by experts in the field
Thematic volume covering material needed for young professionals joining the field of research and graduate students taking survey courses
Up-to-date research on signaling systems and mutations in transcription factors that provide new targets for treating disease

The cyanobacteria are a fascinating group of bacteria that have adapted to colonize almost every environment on the planet. They are the only prokaryotes capable of oxygenic photosynthesis, responsible for up to 20-30% of Earth's photosynthetic productivity. They can attune their light-harvesting systems to changes in available light conditions, fix nitrogen, and have circadian rhythms. In addition, many cyanobacteria species exhibit gliding mobility and can differentiate into specialized cell types called heterocysts, and some are symbiotic. Thanks to their simple nutritional requirements, their metabolic plasticity, and the powerful genetics of some model strains, cyanobacteria could be exploited for use as microbial cell factories for carbon capture and storage, and for the sustainable production of secondary metabolites and biofuels. Understanding their cell biology is an essential step to achieving this. In this book, leading senior scientists and young researchers review the current key topics in cyanobacterial cell biology to provide a timely overview. Topics covered include: historical background * cell division * the cell envelope * the thylakoid membrane * protein targeting, transport, and translocation * chromatic acclimation * the carboxysome * glycogen as a dynamic storage of photosynthetically fixed carbon * cyanophycin * gas vesicles * motility in unicellular and filamentous cyanobacteria * cellular differentiation in filamentous cyanobacteria * cell-cell joining proteins in heterocyst-forming cyanobacteria. This cutting-edge text will provide a valuable resource for all those working in this field and is recommended for all microbiology libraries.

This must-have cell signaling title will appeal to researchers across molecular biology, biochemistry, cell biology and genetics. The articles are written and edited by experts in the field and emphasize signaling to and from intracellular compartments including transcriptional responses to cytoplasmic and nuclear signaling events, chromatin remodeling and stress responses, the regulation of endoplasmic reticulum function, control of cell cycle progression and apoptosis and the modulation of the activities of mitochondria and other organelles.

Articles written and edited by experts in the field
Thematic volume covering regulation of endoplasmic reticulum function, regulation of cell cycle progression, and quality control and assurance in mitochondrion events
Up-to-date research on events in membrane proteins and proteins of intracellular matrix

A primary component of cell signaling research, this title covers the principal membrane-bound receptor families, including their structural organization. Written and edited by experts in the field, this book provides up-to-date research on transmembrane signaling entities and their initiating responses following extracellular stimulation.

Articles written and edited by experts in the field
Thematic volume covering effectors, cytosolic events, nuclear, and cytoplasmic events
Up-to-date research on signaling systems and mutations in transcription factors that provide new targets for treating disease

This book sheds new light on the physiology, molecular biology and pathophysiology of epithelial ion channels and transporters. It combines the basic cellular models and functions by means of a compelling clinical perspective, addressing aspects from the laboratory bench to the bedside. The individual chapters, written by leading scientists and clinicians, explore specific ion channels and transporters located in the epithelial tissues of the kidney, intestine, pancreas and respiratory tract, all of which play a crucial part in maintaining homeostasis. Further topics include the fundamentals of epithelial transport; mathematical modeling of ion transport; cell volume regulation; membrane protein folding and trafficking; transepithelial transport functions; and lastly, a discussion of transport proteins as potential pharmacological targets with a focus on the pharmacology of potassium channels.

The volumes in this authoritative series present a multidisciplinary approach to modeling and simulation of flows in the cardiovascular and ventilatory systems, especially multiscale modeling and coupled simulations. The cardiovascular and respiratory systems are tightly coupled, as their primary function is to supply oxygen to and remove carbon dioxide from the body's cells. Because physiological conduits have deformable and reactive walls, macroscopic flow behavior and prediction must be coupled to nano- and microscopic events in a corrector scheme of regulated mechanisms when the vessel lumen caliber varies markedly. Therefore, investigation of flows of blood and air in physiological conduits requires an understanding of the biology, chemistry, and physics of these systems together with the mathematical tools to describe their functioning.
Volume 3 is devoted to the set of mediators of the cell surface, especially ion and molecular carriers and catalytic receptors that, once liganded and activated, initiate signal transduction pathways. Intracellular cascades of chemical reactions trigger the release of substances stored in cellular organelles and/or gene transcription and protein synthesis. Primary mediators are included in models of regulated cellular processes, but multiple secondary signaling components are discarded to allow simple, representative modeling and to manage their inverse problems.