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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical) > General

Mycorrhizal Symbiosis (Hardcover, 3rd edition): Sally E. Smith, David J. Read Mycorrhizal Symbiosis (Hardcover, 3rd edition)
Sally E. Smith, David J. Read
R3,240 R2,685 Discovery Miles 26 850 Save R555 (17%) Ships in 10 - 15 working days

The roots of most plants are colonized by symbiotic fungi to form mycorrhiza, which play a critical role in the capture of nutrients from the soil and therefore in plant nutrition. "Mycorrhizal Symbiosis" is recognized as the definitive work in this area. Since the last edition was published there have been major advances in the field, particularly in the area of molecular biology, and the new edition has been fully revised and updated to incorporate these exciting new developments.
Over 50% new material
Includes expanded colour plate section
Covers all aspects of mycorrhiza
Presents new taxonomy
Discusses the impact of proteomics and genomics on research in this area

Fungi in Ecosystem Processes (Paperback, 2nd edition): John Dighton Fungi in Ecosystem Processes (Paperback, 2nd edition)
John Dighton
R1,575 Discovery Miles 15 750 Ships in 10 - 15 working days

This new edition of Fungi in Ecosystem Processes continues the unique approach of examining the roles of fungi from the perspective of ecosystem functions. It explores how fungi have adapted to survive within particular constraints, how they help to maintain homeostasis in ecosystems, how they facilitate resistance to perturbations, and how they influence the communities of other organisms. Updated and revised, the second edition Expands the section on plant pathogens, invasive species, and insect-fungal interactions Provides more extensive coverage on insect-fungal interactions, including entomopathogens, the links between entomopathogens and endophytes, and symbiotic and mutualistic interactions Adds a new section on fungi in the built environment Presents new material on below-ground to above-ground interactions mediated through fungi, such as mycorrhizal signaling systems for herbivory defense The book also includes expanded coverage of the role of fungi in suppressive soils, aquatic and marine fungi, modern methods of following food chains in fungal-invertebrate trophic interactions, and the physiology of nutrient uptake by mycorrhizae. A necessary update and expansion to previous material, this book provides an essential reference on the current understanding of fungal roles in ecosystem processes. It also identifies directions for future study, including an emphasis on the need for further research on fungi in built environments.

Food Safety in Poultry Meat Production (Hardcover, 1st ed. 2019): Kumar Venkitanarayanan, Siddhartha Thakur, Steven C. Ricke Food Safety in Poultry Meat Production (Hardcover, 1st ed. 2019)
Kumar Venkitanarayanan, Siddhartha Thakur, Steven C. Ricke
R5,855 Discovery Miles 58 550 Ships in 18 - 22 working days

This comprehensive study of poultry meat safety offers readers the most up-to-date information on food safety concerns in poultry meat production. Chapters address recent topics of interest such as organic poultry production, antimicrobial resistant pathogens in poultry, antibiotic usage in poultry production, and pre- and post- harvest approaches to improving poultry meat safety. The last couple of decades have observed a significant increase in poultry meat production in the US. However, poultry meat is a potential source of foodborne pathogens such as Salmonella, Campylobacter spp. and pathogenic Escherichia coli (APEC linked to human infections), leading to economic losses to the poultry industry and impacting public health. Advances in knowledge in microbiology, molecular biology, immunology and "omics" fields have intensified efforts to improve the microbiological safety of poultry by targeting virulence mechanisms of the pathogens, developing vaccines and improving gut health in chickens. Moreover, due to the emergence of multidrug resistance in poultry-borne pathogens, and growth of organic poultry production, there exists significant interest for developing natural strategies for controlling pathogens in chickens. This edited volume provides insight into these strategies and covers other material of interest to food microbiologists, public health personnel, and poultry scientists. Readers of various backgrounds will appreciate its incorporation of recent developments not covered in other publications on the subject.

Beneficial Microorganisms in Multicellular Life Forms (Hardcover, 2011 Ed.): Eugene Rosenberg, Uri Gophna Beneficial Microorganisms in Multicellular Life Forms (Hardcover, 2011 Ed.)
Eugene Rosenberg, Uri Gophna
R4,057 Discovery Miles 40 570 Ships in 18 - 22 working days

All animals and plants form associations with hundreds or thousands of different beneficial microorganisms. These symbiotic microbes play an important role in the development, adaptation, health and evolution of their hosts. This book brings together a group of diverse biologists to discuss microbial interactions with multicellular life forms including insects, corals, plants, and mammals, including humans. The various mechanisms by which microorganisms benefit their hosts are discussed, including providing essential nutrients, preventing disease, inducing the immune system, and combating stress. Since the microbiota can be transferred from parent to offspring, it plays an important role in the origin and evolution of animal and plant species. This book should be of interest to the widest range of biological scientists, merging the studies of host and microbial physiology, symbiosis, and the ecology and evolution of symbiotic partners.

Reproduction in Fungi (Hardcover, 1994 ed.): C. Elliot Reproduction in Fungi (Hardcover, 1994 ed.)
C. Elliot
R5,313 Discovery Miles 53 130 Ships in 18 - 22 working days

The main theme of this book is how reproduction in fungi is controlled by genetic and environmental factors. The genetics of fungi is at a crossroads - the methods of classical genetics are giving way to those of recombinant DNA technology. Reproduction in Fungi takes stock of what has been learned to date and points the way to future research.

Schistosoma - Biology, Pathology and Control (Paperback): Barrie G.M. Jamieson Schistosoma - Biology, Pathology and Control (Paperback)
Barrie G.M. Jamieson
R1,590 Discovery Miles 15 900 Ships in 10 - 15 working days

Apart from malaria, schistosomiasis is the most prevalent parasitic infection in the world. It affects more than 200 million people in 76 tropical and subtropical countries, causing great suffering and resulting in thousands of deaths. Written by world authorities, this book examines many aspects of the biology, pathology, and control of the schistosoma parasite. Ranging in topic from infection in Pharaonic Egypt, through DNA relationships and biological systems, to advances in development of vaccines against the parasite, this book is a comprehensive text written for researchers and medical professionals alike.

Microbial Biofilms - Omics Biology, Antimicrobials and Clinical Implications (Paperback): Chaminda Jayampath Seneviratne Microbial Biofilms - Omics Biology, Antimicrobials and Clinical Implications (Paperback)
Chaminda Jayampath Seneviratne
R1,559 Discovery Miles 15 590 Ships in 10 - 15 working days

Microbial Biofilms: Omics Biology, Antimicrobials and Clinical Implications is a comprehensive survey of microbial biofilms and their role in human health and disease with contributions from world renowned experts in molecular microbiology, proteomics, genomics, metabolomics and infectious diseases. The book is intended to serve as a guide for students, as well as a reference for researchers, clinicians and industry professionals. The chapters cover bacterial and fungal microbiomes, and the latest omics techniques organized in a clear and up-to-date manner. One of the highlights of this book is the comprehensive information on "omics of microbial biofilms". The chapters dedicated to metagenomics, proteomics and metabolomics are designed to provide a simple and holistic review of the current knowledge and, the applications of these techniques in the field of microbial biofilms. In addition to introductory chapters on microbial biofilms and their clinical implications, subsequent chapters delve into oral biofilms, their composition, and metagenomic diversity. Thereafter, mechanisms of drug resistance in microbial biofilms are reviewed, as well as the proteomic and metabolomic characterization of this resistance. The book includes a comprehensive discussion of persister cells and host-microbial interactions on mucosal surfaces. Finally, the book concludes with a summary of novel therapeutic approaches for biofilms such as synbiotics and biogenics.

Stress in Yeasts and Filamentous Fungi, Volume 27 (Hardcover): Simon Avery, Malcolm Stratford, Pieter van West Stress in Yeasts and Filamentous Fungi, Volume 27 (Hardcover)
Simon Avery, Malcolm Stratford, Pieter van West
R3,451 Discovery Miles 34 510 Ships in 10 - 15 working days

Yeasts and filamentous fungi need to cope with stress, whether growing in the laboratory or in the natural environment, whether victims or offenders in interactions with other organisms. These considerations are discussed in this volume that coveres stress in the broad sense, within the context of mycology.
* Includes discussions of the stresses associated with organism-organism interactions and stress under controlled conditions
* Anthropogenic stress towards fungi in the environment and the impacts that such stressors may have on different organisms and communities in the wild are explained
* Encompasses a breadth of information from the bigger picture of stress effects on fungi in their natural habitats, to the recent advances in underlying molecular-level understanding

Extremophiles: Applications in Nanotechnology (Hardcover, 1st ed. 2016): Sonia Tiquia-Arashiro, Debora Frigi Rodrigues Extremophiles: Applications in Nanotechnology (Hardcover, 1st ed. 2016)
Sonia Tiquia-Arashiro, Debora Frigi Rodrigues
R3,864 R3,311 Discovery Miles 33 110 Save R553 (14%) Ships in 10 - 15 working days

This book discusses the extremophiles explored for biosynthesis of nanoparticles. Nanotechnology is a widely emerging field involving interdisciplinary subjects such as biology, physics, chemistry and medicine. A wide variety of microorganisms, such as bacteria, fungi and algae are employed as biological agents for the synthesis of nanoparticles. Novel routes by which extremophiles can be employed to generate nanoparticles have yet to be discovered. The book is divided into 5 major chapters: (1) Major types of nanoparticles in nanotechnology (2) Diversity of microbes in the synthesis of nanoparticles (3) Extremophiles in nanoparticle biosynthesis (4) Applications of nanoparticles produced by extremophiles (5) Challenges and Future perspectives

Ecology of Saprotrophic Basidiomycetes, Volume 28 (Hardcover): Lynne Boddy, Juliet Frankland, Pieter van West Ecology of Saprotrophic Basidiomycetes, Volume 28 (Hardcover)
Lynne Boddy, Juliet Frankland, Pieter van West
R3,586 Discovery Miles 35 860 Ships in 10 - 15 working days

The breadth and depth of understanding of many areas concerning basidiomycetes has increased dramatically since the premier publication of Frankland et al., Decomposer Basidiomycetes: their Biology and Ecology. New vistas have opened up with the advent of powerful computing, modeling and molecular approaches helping to greatly increase the general understanding of the ecology of basidiomycetes. This is tantamount to understanding the role of fungi in natural ecosystems because they are major agents of decomposition and nutrient cycling. These remarkable advances have been incorporated into this volume that discusses all aspects of saprotrophic basidiomycete ecology.

Dairy Processing: Advanced Research to Applications (Hardcover, 1st ed. 2020): Jagrani Minj, Aparna Sudhakaran V, Anuradha... Dairy Processing: Advanced Research to Applications (Hardcover, 1st ed. 2020)
Jagrani Minj, Aparna Sudhakaran V, Anuradha Kumari
R4,739 Discovery Miles 47 390 Ships in 18 - 22 working days

This book focuses on advanced research and technologies in dairy processing, one of the most important branches of the food industry. It addresses various topics, ranging from the basics of dairy technology to the opportunities and challenges in the industry. Following an introduction to dairy processing, the book takes readers through various aspects of dairy engineering, such as dairy-based peptides, novel milk products and bio-fortification. It also describes the essential role of microorganisms in the industry and ways to detect them, as well as the use of prebiotics, and food safety. Lastly, the book examines the challenges faced, especially in terms of maintaining quality across the supply chain. Covering all significant areas of dairy science and processing, this interesting and informative book is a valuable resource for post-graduate students, research scholars and industry experts.

Advances in Microbial Physiology, Volume 52 (Hardcover, 52nd edition): Robert K. Poole Advances in Microbial Physiology, Volume 52 (Hardcover, 52nd edition)
Robert K. Poole
R3,912 Discovery Miles 39 120 Ships in 10 - 15 working days

Advances in Microbial Physiology is one of the most successful and prestigious series from Academic Press, an imprint of Elsevier. It publishes topical and important reviews, interpreting physiology to include all material that contributes to our understanding of how microorganisms and their component parts work.
First published in 1967, it is now in its 50th volume. The Editors have always striven to interpret microbial physiology in the broadest context and have never restricted the contents to traditional views of whole cell physiology. Now edited by Professor Robert Poole, University of Sheffield, Advances in Microbial Physiology continues to be an influential and very well reviewed series.
* In 2004, the Institute for Scientific Information released figures showing that the series had an Impact Factor of 8.947, with a half-life of 6.3 years, placing it 5th in the highly competitive category of Microbiology

Microbial Biosurfactants - Preparation, Properties and Applications (Hardcover, 1st ed. 2021): Inam Uddin, Mohd Imran Ahamed,... Microbial Biosurfactants - Preparation, Properties and Applications (Hardcover, 1st ed. 2021)
Inam Uddin, Mohd Imran Ahamed, Ramprasad
R5,179 Discovery Miles 51 790 Ships in 18 - 22 working days

Microbial biosurfactant compounds are a group of structurally diverse molecules produced by microorganisms, and are mainly categorized according to their chemical structure. The diversity of microbial biosurfactants makes them versatile and means that they offer a range of capabilities, while at the same time being economically sustainable. As such, they have potential applications in environmental processes, as well as in food, biomedicine and other industries. This book discusses innovative approaches and cutting-edge research that utilize the various properties of biosurfactants. Drawing on research from around the globe, it provides an up-to-date review of biosurfactant applications and their importance in fields such as medicine, gene therapy, immunotherapy, antimicrobial bioremediation and agriculture. It also discusses their anti-adhesive properties. The book will appeal to academics and researchers in the field of microbiology, as well as policymakers. It also serves as additional reading material for undergraduate and graduate students of agriculture, ecology, soil science, and environmental sciences.

Advances in Microbial Physiology, Volume 51 (Hardcover, 51st edition): Robert K. Poole Advances in Microbial Physiology, Volume 51 (Hardcover, 51st edition)
Robert K. Poole
R3,900 Discovery Miles 39 000 Ships in 10 - 15 working days

Advances in Microbial Physiology is one of the most successful and prestigious series from Academic Press, an imprint of Elsevier. It publishes topical and important reviews, interpreting physiology to include all material that contributes to our understanding of how microorganisms and their component parts work.
First published in 1967, it is now in its 50th volume. The Editors have always striven to interpret microbial physiology in the broadest context and have never restricted the contents to "traditional" views of whole cell physiology. Now edited by Professor Robert Poole, University of Sheffield, Advances in Microbial Physiology continues to be an influential and very well reviewed series.
* In 2004, the Institute for Scientific Information released figures showing that the series had an Impact Factor of 8.947, with a half-life of 6.3 years, placing it 5th in the highly competitive category of Microbiology.

Biofuel Technologies - Recent Developments (Hardcover, 2013 ed.): Vijai Kumar Gupta, Maria G. Tuohy Biofuel Technologies - Recent Developments (Hardcover, 2013 ed.)
Vijai Kumar Gupta, Maria G. Tuohy
R6,576 Discovery Miles 65 760 Ships in 10 - 15 working days

Biofuels are considered to be the main potential replacement for fossil fuels in the near future. In this book international experts present recent advances in biofuel research and related technologies. Topics include biomethane and biobutanol production, microbial fuel cells, feedstock production, biomass pre-treatment, enzyme hydrolysis, genetic manipulation of microbial cells and their application in the biofuels industry, bioreactor systems, and economical processing technologies for biofuel residues. The chapters provide concise information to help understand the technology-related implications of biofuels development. Moreover, recent updates on biofuel feedstocks, biofuel types, associated co- and byproducts and their applications are highlighted. The book addresses the needs of postgraduate researchers and scientists across diverse disciplines and industrial sectors in which biofuel technologies and related research and experimentation are pursued.

Advanced Quantitative Microbiology for Foods and Biosystems - Models for Predicting Growth and Inactivation (Hardcover): Micha... Advanced Quantitative Microbiology for Foods and Biosystems - Models for Predicting Growth and Inactivation (Hardcover)
Micha Peleg
R6,786 Discovery Miles 67 860 Ships in 10 - 15 working days

Presenting a novel view of the quantitative modeling of microbial growth and inactivation patterns in food, water, and biosystems, Advanced Quantitative Microbiology for Foods and Biosystems: Models for Predicting Growth and Inactivation describes new models for estimating microbial growth and survival. The author covers traditional and alternative models, thermal and non-thermal preservation, water disinfection, microbial dose response curves, interpretation of irregular count records, and how to estimate the frequencies of future outbursts. He focuses primarily on the mathematical forms of the proposed alternative models and on the rationale for their introduction as substitutes to those currently in use.

The book provides examples of how some of the methods can be implemented to follow or predict microbial growth and inactivation patterns, in real time, with free programs posted on the web, written in MS ExcelO, and examples of how microbial survival parameters can be derived directly from non-isothermal inactivation data and then used to predict the efficacy of other non-isothermal heat treatments. Featuring numerous illustrations, equations, tables, and figures, the book elucidates a new approach that resolves several outstanding issues in microbial modeling and eliminates inconsistencies often found in current methods.

Fungi in Sustainable Food Production (Hardcover, 1st ed. 2021): Xiaofeng Dai, Minaxi Sharma, Jieyin Chen Fungi in Sustainable Food Production (Hardcover, 1st ed. 2021)
Xiaofeng Dai, Minaxi Sharma, Jieyin Chen
R2,669 Discovery Miles 26 690 Ships in 18 - 22 working days

This book presents research on the challenges and potential of fungal contribution in agriculture for food substantiality. Research on fungi plays an essential role in the improvement of biotechnologies which lead global sustainable food production. Use of fungal processes and products can bring increased sustainability through more efficient use of natural resources. Fungal inoculum, introduced into soil together with seed, can promote more robust plant growth through increasing plant uptake of nutrients and water, with plant robustness being of central importance in maintaining crop yields. Fungi are one of nature's best candidates for the discovery of food ingredients, new drugs and antimicrobials. As fungi and their related biomolecules are increasingly characterized, they have turned into a subject of expanding significance. The metabolic versatility makes fungi interesting objects for a range of economically important food biotechnology and related applications. The potential of fungi for a more sustainable world must be realized to address global challenges of climate change, higher demands on natural resources.

Phosphate Solubilizing Microorganisms - Principles and Application of Microphos Technology (Hardcover, 2014 ed.): Mohammad... Phosphate Solubilizing Microorganisms - Principles and Application of Microphos Technology (Hardcover, 2014 ed.)
Mohammad Saghir Khan, Almas Zaidi, Javed Musarrat
R4,486 R3,415 Discovery Miles 34 150 Save R1,071 (24%) Ships in 10 - 15 working days

This book provides a comprehensive description of phosphate solubilizing microorganisms and highlights methods for the use of microphos in different crop production systems. The focus is on understanding both the basic and applied aspects of phosphate solubilizing microorganisms and how phosphorus-deficient soils can be transformed into phosphorus-rich ones by applying phosphate solubilizing microorganisms. The interaction of rhizosphere phosphate solubilizing microorganisms and environmental variables, as well as their importance in the production of crops such as legumes, cereals, vegetables etc. are discussed and considered. The use of cold-tolerant phosphate solubilizing microorganisms to enhance crop productivity in mountainous regions is examined, as are the ecological diversity and biotechnological implications of phosphate solubilizing microorganisms. Lastly, the role of phosphate solubilizing microorganisms in aerobic rice cultivation is highlighted. This volume offers a broad overview of plant disease management using phosphate solubilizing microbes and presents strategies for the management of cultivated crops. It will therefore be of special interest to both academics and professionals working in the fields of microbiology, soil microbiology, biotechnology and agronomy, as well as the plant protection sciences. This timely reference book provides an essential and comprehensive source of material, as it includes recent findings on phosphate solubilizing microorganisms and their role in crop production.

Beneficial Microbes in Agro-Ecology - Bacteria and Fungi (Paperback): N. Amaresan, M. Senthilkumar, K. Annapurna, Krishna... Beneficial Microbes in Agro-Ecology - Bacteria and Fungi (Paperback)
N. Amaresan, M. Senthilkumar, K. Annapurna, Krishna Kumar, N. Sankaranaryanan
R7,915 Discovery Miles 79 150 Ships in 10 - 15 working days

Beneficial Microbes in Agro-Ecology: Bacteria and Fungi is a complete resource on the agriculturally important beneficial microflora used in agricultural production technologies. Included are 30 different bacterial genera relevant in the sustainability, mechanisms, and beneficial natural processes that enhance soil fertility and plant growth. The second part of the book discusses 23 fungal genera used in agriculture for the management of plant diseases and plant growth promotion. Covering a wide range of bacteria and fungi on biocontrol and plant growth promoting properties, the book will help researchers, academics and advanced students in agro-ecology, plant microbiology, pathology, entomology, and nematology.

Molecular Microbial Ecology (Paperback, New edition): Mark Osborn, Cindy Smith Molecular Microbial Ecology (Paperback, New edition)
Mark Osborn, Cindy Smith
R2,254 Discovery Miles 22 540 Ships in 10 - 15 working days

Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary method of culturing microorganisms on media allowed us to study only between 0.1 and 10% of the total microbial flora in any given environment. Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world's leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.

Advances in Microbial Physiology, Volume 50 (Hardcover, 52 Ed): Robert K. Poole Advances in Microbial Physiology, Volume 50 (Hardcover, 52 Ed)
Robert K. Poole
R3,906 Discovery Miles 39 060 Ships in 10 - 15 working days

Advances in Microbial Physiology is one of the most successful and prestigious series from Academic Press, an imprint of Elsevier. It publishes topical and important reviews, interpreting physiology to include all material that contributes to our understanding of how microorganisms and their component parts work.
First published in 1967, it is now in its 50th volume. The Editors have always striven to interpret microbial physiology in the broadest context and have never restricted the contents to traditional views of whole cell physiology. Now edited by Professor Robert Poole, University of Sheffield, Advances in Microbial Physiology continues to be an influential and very well reviewed series.
* In 2004, the Institute for Scientific Information released figures showing that the series had an Impact Factor of 8.947, with a half-life of 6.3 years, placing it 5th in the highly competitive category of Microbiology.

Advances in Microbial Ecology, v. 13 (Hardcover): J.G. Jones Advances in Microbial Ecology, v. 13 (Hardcover)
J.G. Jones
R2,459 Discovery Miles 24 590 Ships in 10 - 15 working days

Implications of Resource-Ratio Theory for Microbial Ecology; V.H. Smith. 13C Tracer Methodology in Microbial Ecology with Special Reference to Primary Production Processes in Aquatic Environments; T. Hama, et al. Sex in Ciliates; F. Dini, D. Nyberg. Microbial Ecology in Lake Ciso; C. PedroAlio, R. Guerrero. Biological Activities of Symbiotic and Parasitic Protists in Low Oxygen Environments; A.G. Williams, D. Lloyd. Polymorphism in Bacteria; P.B. Rainey, et al. Decomposition of Shoots of a Saltmarsh Grass; S.Y. Newell. Dynamics of Autotrophic Picoplankton in Marine and Freshwater Ecosystems; T. Weisse. Bacterial Growth Rates and Production As Determined by [3HMethyl]thymidine? R.D. Robarts, T. Zohary. Index.

Pharmacology and Pathophysiology of the Control of Breathing (Hardcover): Denham S. Ward, Albert Dahan, Luc Teppema Pharmacology and Pathophysiology of the Control of Breathing (Hardcover)
Denham S. Ward, Albert Dahan, Luc Teppema
R4,823 R4,548 Discovery Miles 45 480 Save R275 (6%) Ships in 10 - 15 working days

Exploring the functional anatomy and physiology of the ventilatory control system from the intracellular to the integrative level, this references serves as the first source to offer comprehensive coverage of the influences of various pharmacological agents on the control of breathing.

Real-time PCR in Microbiology - From Diagnosis to Characterization (Hardcover): Ian M. Mackay Real-time PCR in Microbiology - From Diagnosis to Characterization (Hardcover)
Ian M. Mackay
R5,259 Discovery Miles 52 590 Ships in 18 - 22 working days

Real-time PCR has established itself as a sensitive and specific qualitative and quantitative technique that has become important to all areas of microbiology. This invaluable book describes and explains some of the more complex aspects of real-time PCR presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardisation, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion providing an insightful, topical and interesting discourse with contributions from over 30 authorities and experts on real-time PCR. The editor and authors have produced an excellent book that will be extremely useful for all microbiologists. It is a recommended book for all microbiology laboratories.

Molecular Alternatives to Indicator and Pathogen Detection (Paperback, Illustrated Ed): S. C. Jiang Molecular Alternatives to Indicator and Pathogen Detection (Paperback, Illustrated Ed)
S. C. Jiang
R3,940 R3,612 Discovery Miles 36 120 Save R328 (8%) Ships in 9 - 17 working days

Microbial quality of water is a prime public health concern in today's world. To protect public health, the World Health Organization and the U.S. Environmental Protection Agency have established microbial pollution indicator standards and recommended routine monitoring of water for both total and fecal coliforms (Dufour, 1984, U.S. EPA 1986,1994, WHO 1993). However, the adequacy of current water quality standards to indicate the presence or absence of human pathogens is still questionable. For example, human viruses are more resistant to sewage treatment processes and environmental conditions than bacterial indicators and therefore may pose a substantial threat. It is now recognized that the absence, or a low concentration, of indicator organisms in water may not adequately reflect the absence of human viruses. In our previous study of southern California coastal waters, we found over 30% of coastal waters tested contained human viruses, and the presence of these viruses did not correlate with an elevated level of bacterial indicators (Jiang et al. 2000). The goal of this research is to develop and validate a molecular method for rapid and specific detection of microbial contaminants including human viruses and bacterial indicators in treated sewage effluents and receiving waters. Research Outcomes An extensive search and review of current state of technology for molecular alternatives to indicator and viral pathogen detection was conducted. The results indicate that although real-time PCR methods have been widely applied in the clinical research for detection of human viruses, environmental application of this method is very limited. In addition, there is an urgent need for a method for efficient concentration and purification of human viruses in complex environmental matrixes. During this study, we have designed, tested and optimized real-time quantitative PCR method using specific and degenerate primers and probes targeting at adenoviruses and enterococci, respectively. Experimental testing of real time PCR primers and probes for adenoviruses demonstrated reproducible results at efficiency greater than 90% over a 5-log dynamic range of target concentration. The enterococci real time PCR was efficient at 99% of the time and over a 7-log dynamic range of target concentration. Application of these methods to sewage effluents and coastal waters demonstrated that real-time PCR methods are more sensitive than culturing methods at detection of targets, suggesting a great potential for real-time quantification of microbial contaminants in environments. However, real-time PCR based method, like other genome based detection technology, overestimates the concentration of infectious viral concentration in the environment. In addition to the method development for real-time PCR detection, we have also sampled and isolated human adenoviruses from Newport Bay, California using human embryonic kidney cells, 293A. Cloning and sequencing of selective environmental adenovirus hexon gene suggested that most of the viruses recovered from the environment belong to adenovirus serotype 40 (66%). This result is in agreement with clinical data on the load of viral shedding in feces. Since adenovirus serotypes 40 and 41 are the major cause of childhood diarrhea, the result of this investigation indicates the importance of monitoring water for viral quality. Comparison of four different tissue culture cell lines for their sensitivities to adenoviruses infection has demonstrated that the genetically engineered 293A cells are the most efficient at recovery of adenoviruses 40. This result is a significant contribution to our ability to assay for infectious adenoviruses in environmental samples.

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