This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.

This book offers a range of environmentally benign molecular mechanisms which are safer alternative strategies for effective insect pest management. In modern era of biotechnology, there has been much advancement in the field of molecular biology, where many more techniques have evolved which can be helpful in the field of pest management too. Plant resistance, development of transgenic plants, and many more techniques are being considered the panacea to pest problems. On the other hand, there are wide spread concerns of the safety of biotechnological interventions with nontarget organisms including humans. While the world stands divided on the ethical issues of these approaches and the many safety concerns, scientists believe that well thought of biotechnological interventions are probably the only safest ways possible for reducing pest attacks on crops. It explores various techniques and aspects related to molecular pathways for crop pest control. This book is a useful resource for postgraduate students and researchers of agriculture sciences, plant pathology and plant physiology. It is also useful for policy planners in agriculture.

This book brings together specialized information on modern aspects of applied microbiology in pest management. In the last few decades, the humans have witnessed major advancements in Life Sciences, as a result several new and powerful tools and techniques have evolved. This has led to great advancements in microbial nutrition, genetics and their application in different fields. In modern era of biotechnology, the microbes have provided solutions to many of the human problems and necessities and thus serve as human and farmers' friends. The microbes have proved to be successful tools for the pest management. Similarly, there has been much advancement in the field of molecular biology, where many more techniques have evolved which can be helpful in the field of pest management too. Plant resistance, development of transgenic plants, and many more techniques are being considered the panacea to pest problems. On the other hand, there are wide spread concerns of the safety of these microbial and biotechnological interventions with nontarget organisms including humans. While the world stands divided on the ethical issues of these approaches and the many safety concerns, scientists believe that well thought of microbial and biotechnological interventions are probably the only safest ways possible for reducing pest attacks on crops. This is useful read for postgraduate students and teachers, plant protection practioners across the world and also useful for policy planners.

This book provides a comprehensive monograph of the family Humiraceae. It includes information on economic botany, conservation, phylogenetic relationships, taxonomic history, ecology, cytology, anatomy, and phytochemistry, among other topics. This volume is illustrated with line drawings, black and white photographs, and distribution maps. It was written by the world-leading authority on this plant group and contains a total of eight genera, 65 species, and 15 infraspecific taxa, with two new species described. This work is volume 123 in the Flora Neotropica book series (Lawrence M. Kelly, Editor-in-Chief). Flora Neotropica volumes provide taxonomic treatments of plant groups or families growing in the Americas between the Tropic of Cancer and the Tropic of Capricorn.

This volume provides a collection of molecular protocols detailing the most common and modern techniques on fusarium wilt. Chapters guide readers through methods on initial isolation, molecular-based identification, genome characterization, generation of mutants, and characterization of interactions with other organisms including host plants. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Fusarium wilt: Methods and Protocols aims to be a valuable resource for mycologists, plant pathologists, microbiologists, geneticists, and other scientists that have an interest in members of the Fusarium oxysporum species complex or closely related fungi.

The book is presenting a comprehensive information on fundamental, and applied knowledge of Plasmodiophora brassicae Woronin. infecting cruciferous crops, and weeds. Clubroot of crucifers has spread over more than 88 countries of the world with average annual loss of cruciferous crops from 10-15 per cent at global level. It is considered as a disease of cultivation since once introduced in a field, its inoculum piles up year by year in the form of resilient resting spores of P. brassicae which spreads in the field through field operations. This disease is very unique since the pathogen can survive in the soil in the rhizosphere of non-host plants in addition to its main host cruciferous species, cultivated or wild. This book complies inclusive information about the disease, its geographical distribution, symptoms, host range, yield losses, and disease assessment scales. The book also explores host-parasite interactions in the form of seed infection, disease cycle, process of infection, pathogenesis, epidemiology and forecasting. Chapters discuss the genetic and molecular mechanisms of host-parasite relationships, management practices including cultural, chemical, biological control practices, and other integrated approaches. The book is immensely useful to researchers, teachers, extension specialists, farmers, and all others who are interested to grow healthy and profitable cruciferous crops all over the world. Also the book serves as additional reading material for undergraduate and graduate students of agriculture and especially plant pathology. National and international agricultural scientists, policy makers will also find this to be a useful read.

This edited book provides knowledge about hemicelluloses biorefinery approaching production life cycle, circular economy, and valorization by obtaining value-added bioproducts and bioenergy. A special focus is dedicated to chemical and biochemical compounds produced from the hemicelluloses derivatives platform. Hemicelluloses are polysaccharides located into plant cell wall, with diverse chemical structures and properties. It is the second most spread organic polymer on nature and found in vast lignocellulosic materials from agro and industrial wastes, therefore, hemicelluloses are considered as abundant and renewable raw material/feedstock. Biorefinery concept contributes to hemicelluloses production associated with biomass industrial processes. Hemicelluloses are alternative sources of sugars for renewable fuels and as platform for chemicals production. This book reviews chemical processes for sugar production and degradation, obtaining of intermediate and final products, and challenges for pentose fermentation. Aspects of hemicelluloses chain chemical and enzymatic modifications are presented with focus on physicochemical properties improvement for bioplastic and biomaterial approaches. Hemicelluloses are presented as sources for advanced materials in biomedical and pharmaceutical uses, and as hydrogel for chemical and medicine deliveries. An interdisciplinary approach is needed to cover all the processes involving hemicelluloses, its conversion into final and intermediate value-added compounds, and bioenergy production. Covering this context, this book is of interest to teachers, students, researchers, and scientists dedicated to biomass valorization. This book is a knowledge source of basic aspects to advanced processing and application for graduate students, particularly. Besides, the book serves as additional reading material for undergraduate students (from different courses) with a deep interest in biomass and waste conversion, valorization, and chemical products from hemicelluloses.

This open access book in the field of plant pest detection shows a constant demand in development and improvement of fast and reliable detection tools, especially for high-priority pests. This open access book describes and summarizes the whole process of the organization of test performance study (TPS) for these tools. The outcome of TPS, obtained through the evaluation of the performance of one or more diagnostic tests by several laboratories on defined samples, is the finding of the best performing test/s for particular pest and for specific uses. Nowadays the intensification of worldwide trade and associated controls increases the need for quality assurance accreditation and harmonization of laboratories practices. Therefore, such studies are very important, but, non-existent. Considering those facts, our goal was to develop guidelines, by using the data and experiences of involved partners, for further TPS in the field of plant health. Developed guidelines could be easily transferable to other microbiology fields.

Plant galls may be produced by a wide variety of organisms, from fungi to parasitic insects, on an equally wide variety of hosts. Their taxonomy is highly complex, as are the life cycles of the organisms associated with them. Yet, common as they are, plant galls are often poorly understood. This book brings together information from the diverse disciplines involved in the study of plant galls: ecology, evolution, molecular biology, physiology, and developmental biology. The work considers the latest issues, covering questions of classification, coevolution, ecology, physiology, and plant genetic engineering. As an up-to-date resource in an area of immense interest and debate, the book will enhance the quality of discussion surrounding these phenomena, across all disciplinary perspectives.

This volume highlights molecular methods to study the phytopathogenic rice blast fungus Magnaporthe oryzae. Chapters in this book cover the history, development, and evolution of the pathogen; molecular methods to increase the knowledge of the biology, genetic, and metabolic diversity of the pathogen; and the pathogen's adaptability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Magnaporthe oryzae: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this developing field.

Bananas are a staple food for over 500 million people and are also an important cash crop. Fusarium wilt, caused by the fungus Fusarium oxysporum f.sp. cubense, is one of the most destructive diseases of banana globally. Since the 1990s, an aggressive variant of this fungus, called Tropical Race 4 (TR4), severely affected banana plantations in Southeast Asia from where it spread to other continents, including Latin America, where the global banana export market is primarily centred. TR4 is a soil borne pathogen making the disease difficult to contain. The Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture implemented a Coordinated Research Project (CRP) 'Efficient Screening Techniques to Identify Mutants with Disease Resistance for Coffee and Banana" (2015-2020). This CRP brought together experts from Asia, Europe and Africa in addition to experts of the Joint FAO/IAEA Centre to develop resistance against TR4 through mutation-assisted breeding. Induced mutagenesis is particularly attractive in case of banana since most cultivated bananas are seedless, thus hampering conventional cross breeding. This Open Access book is a compilation of the protocols developed under the CRP specifically for TR4. The first part covers methods for mutation induction, including the integrated use of innovative single-cell culture with mutagenesis techniques. The book also describes up-to-date phenotypic screening methods for TR4 resistance in banana under field-, greenhouse- and laboratory conditions. Finally, molecular and bioinformatics tools for genome-wide mutation discovery following Next Generation Sequencing are also described. Given the imminent threat of Fusarium Wilt TR4 on banana production globally, it is our hope and intention that the book will serve as a timely reference and guide for banana breeders and pathologists worldwide who are committed to the genetic improvement of banana for Fusarium wilt resistance.

Of the global population of more than 7 billion people, some 800 million do not have enough to eat today. By 2050, the population is expected to exceed 9 billion. It has been estimated that some 15% of food production is lost to plant diseases; in developing countries losses may be much higher. Historically, plant diseases have had catastrophic impact on food production. For example: potato blight caused the Irish famine in 1845; brown spot of rice caused the Great Bengal Famine of 1943; southern corn leaf blight caused a devastating epidemic on the US corn crop in 1970. Food security is threatened by an ongoing sequence of plant diseases, some persistent for decades or centuries, others more opportunistic. Wheat blast and banana xanthomonas wilt are two contrasting examples of many that currently threaten food production. Other emerging diseases will follow. The proposed title aims to provide a synthesis of expert knowledge to address this central challenge to food security for the 21st century. Chapters [5] and [11] are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Molecular plant pathology has directly benefited from advances in modern molecular techniques. These techniques have been applied both to pathogen and plant, enhancing our understanding of the organisms themselves and of the complex interactions which determine compatibility between them and their host plants. This new book and its companion volume represent the first comprehensive guide to the latest molecular techniques as well as the established approaches to the subject. Detailed protocols are included which address a wide range of investigations from plant pathogen isolation and culture, through physiology and biochemistry, to techniques for localizing genes and their products within the cells of the infected plants. Topics in Volume I include pathogens and pathogen manipulation, nucleic acid isolation and hybridization techniques, analysis of gene expression, and in situ hybridization. Researchers in plant science, molecular biology, and related areas will find the two volumes of Molecular Plant Pathology to be an invaluable experimental resource for this exciting and fast moving field, providing a wealth of easy-to-follow protocols supported by expert advice and guidance.

Increasing world population, unpredictable climate and various kind of biotic and abiotic stresses necessitate the sustainable increase in crop production through developing improved cultivars possessing enhanced genetic resilience against all odds. An exploration of these challenges and near possible solution to improve yield is addressed in this book. It comprehensively and coherently reviews the application of various aspect of rapidly growing omics technology including genomics, proteomics, transcriptomics and metabolomics for crop development. It provides detailed examination of how omics can help crop science and introduces the benefits of using these technologies to enhance crop production, resistance and other values. It also provides platform to ponder upon the integrative approach of omics to deal with complex biological problems. The book highlights crop improvement such as yield enhancement, biotic and abiotic resistance, genetic modification, bioremediation, food security etc. It explores how the different omics technology independently and collectively would be used to improve the quantitative and qualitative traits of crop plants. The book is useful for graduate and post-graduate students of life science including researchers who are keen to know about the application of omics technologies in the different area of plant science. This book is also an asset to the modern plant breeders, and agriculture biotechnologist.

This book presents a timely review of the latest advances in rhizosphere biology, which have been facilitated by the application of omics tools. It includes chapters on the use of various omics tools in rhizosphere biology, focusing on understanding plant and soil microbe interactions. The role of proteomics and metagenomics in research on symbiotic association is also discussed in detail. The book also includes chapters on the use of omics tools for the isolation of functional biomolecules from rhizospheric microorganisms. The book's respective sections describe and provide detailed information on important omics tools, such as genomics, transcriptomics, proteomics, metabolomics and meta-epigenomics. In turn, the book promotes and describes the combined use of plant biology, microbial ecology, and soil sciences to design new research strategies and innovative methods in soil biology. Lastly, it highlights the considerable potential of the rhizosphere in terms of crop productivity, bioremediation, ecological engineering, plant nutrition and health, as well as plant adaptation to stress conditions. This book offers both a practical guide and reference source for all scientists working in soil biology, plant pathology, etc. It will also benefit students studying soil microbiology, and researchers studying rhizosphere structure.

Abiotic and biotic stress factors, including drought, salinity, waterlog, temperature extremes, mineral nutrients, heavy metals, plant diseases, nematodes, viruses, and diseases, adversely affect growth as well as yield of crop plants worldwide. Plant growth-promoting microorganisms (PGPM) are receiving increasing attention from agronomists and environmentalists as candidates to develop an effective, eco-friendly, and sustainable alternative to conventional agricultural (e.g., chemical fertilizers and pesticide) and remediation (e.g., chelators-enhanced phytoremediation) methods employed to deal with climate change-induced stresses. Recent studies have shown that plant growth-promoting bacteria (PGPB), rhizobia, arbuscular mycorrhizal fungi (AMF), cyanobacteria have great potentials in the management of various agricultural and environmental problems. This book provides current research of biofertilizers and the role of microorganisms in plant health, with specific emphasis on the mitigating strategies to combat plant stresses.

The search for new strategies of pest control with safer molecules is currently of great importance and interest. Microbe-mediated biological crop protection is an attractive and promising technology with no concern for a negative impact on the environment and biodiversity. Microbial hydrolytic enzymes such as proteases, chitinases, lipases, etc. are attractive for this purpose. They present toxic properties and act synergistically to control pest attacks. Also, some metabolites, that microorganisms produce for their survival or defense, can be explored and exploited for plant protection. The focus of this Volume is on the potential of microbial hydrolytic enzymes and their metabolites in agroecosystem functioning. Subsequent chapters review topics such as microbial hydrolytic enzymes as powerful management tools, chitinases in IPM of agro-horticultural crops, metabolites as pesticides and the importance of the metabolites of entomopathogenic fungi, metabolites and virulence factors. Other topicas include: microbial-based nanoparticles, recombinant DNA technologies to improve the efficacy of microbial insecticides, the effects of entomopathogens on insect predators and parasitoids, and the management of major vegetable insect pests. This Volume provides detailed accounts on the safe use of microbial products for sustainable management of insect pests. Its aim is to build solid foundations for the students, teachers, and researchers interested in eco-friendly management of important insect crop pests.

Selenium plays a significant role in preventing certain types of cancer and cardiovascular diseases. The level of Selenium in the human body depends on its concentration in food. In turn, the content in vegetable crops is a function of the soil-plant system. There are many countries in the world with low Selenium content in the soil. The average daily human intake is thus limited through food chain. Analysis of Selenium status suggests that fortification of the soil substrate with Sodium Selenate, and foliar application to agricultural crops are both effective means of Selenium enrichment. Our intention for this publication is to present the possibilities of augmenting Selenium content by biofortification of soils and plants through differentiated nutrition. In the first part of the monograph, the results of Selenium supplementation in model vegetation experiments are presented. The next part of the monograph presents the results of foliar supplementation of Selenium in field conditions. This book is an outstanding reference source for plant breeders and researchers engaged in biofortification of horticulture crops. It is also beneficial to agricultural companies and other stakeholders.

This edited volume is a comprehensive account of plant diseases and insect pests, plant protection and management for various crops using microbial and biotechnological approaches. The book elucidates the role of biotechnology for the enhancement of crop productivity and management of bacterial and fungal diseases via eco-friendly methods. It discusses crop-pest/ pathogen interaction and utilizing this interaction in a beneficial and sustainable way. This book is of interest to teachers, researchers, plant scientists and plant pathologists. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.

Written in easy to follow language, the book presents cutting-edge agriculturally relevant plant biotechnologies and applications in a manner that is accessible to all. This book updates and introduces the scope and method of plant biotechnologies and molecular breeding within the context of environmental analysis and assessment, a diminishing supply of productive arable land, scarce water resources and climate change. New plant breeding techniques including CRISPR-cas system are now tools to meet these challenges both in developed countries and in developing countries. Ethical issues, intellectual property rights, regulation policies in various countries related to agricultural biotechnology are examined. The rapid developments in plant biotechnology are explained to a large audience with relevant examples. New varieties of crops can be adapted to new climatic conditions in order to reduce pest-associated losses and the adverse abiotic effects

Biological control has become an attractive alternative strategy for the control of plant diseases to reduce the excessive use of agrochemicals and its health hazards. But a significant gap still exists between basic research involving the discovery of a biocontrol agent and its development and implementation under commercial conditions. Because BCAs (unlike chemical a.m.) need to establish, colonize, survive and perform their metabolic activity to control diseases. In order to move a biocontrol agent from the laboratory to the market place requires many different disciplines and people with a variety of expertise. Research can stimulate the development of commercial biocontrol agents. Chapter 16 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Plant growth and development is closely dependent on the plant environment, including the wide-spread presence of organic and inorganic xenobiotics and pollutants. Currently, heavy metals are the most common inorganic environmental pollutants and they have pronounced effects and consequences not only for plants, but also for the ecosystem in which the plants form an integral component. It has been suggested that these contaminants accumulate in agricultural crops, thus entering the food chain and posing a significant health risk. Plants growing in polluted sites exhibit altered metabolism, reduced growth, and decreased biomass production. These pollutants adhere to plant roots and exert physical or chemical toxicity and subsequently cell death in plants. Yet, plants have developed various defence mechanisms to counteract the toxicity induced by heavy metals. Only detailed study of the processes and mechanisms would allow researchers and students to understand the interactions, responses, and adaptations of plants to these pollutants; however, there are several unresolved issues and challenges regarding the interaction and biological effects of heavy metals. Therefore, this volume provides relevant, state-of-the-art findings on environmental phytotoxicity and the mechanisms of such interactions at the cellular and molecular levels. This volume consists of chapters on relevant topics contributed by different experts or group of experts so as to make available a comprehensive treatise designed to provide an in-depth analysis of heavy metals phytotoxicity. This book may serve as a reference to scientists, researchers and students in the fields of toxicology, environmental toxicology, phytotoxicology, plant biology, plant physiology, plant biochemistry and plant molecular biology, and especially those interested in heavy metals toxicology.

This landmark reference contains the global sorghum and millet pathology community's decennial summary of diseases and work in progress based primarily on contributions to the Third Global Conference on Sorghum and Millets Diseases in September 2000, sponsored by INTSORMIL, ICRISAT, and INIFAP.

Editor John Leslie has expanded the book's coverage beyond the conference presentations to guarantee a well-rounded treatment of sorghum and millet diseases. One hundred and ninety-seven authors from thirty countries contribute to a truly global picture.

This critical review provides a unique, long-term analysis of different issues during the last quarter century, a summary of the current critical problems, and an evaluation of the sorghum and millet community's progress since its previous meeting in 1988. Perhaps the book's greatest value lies in defining the research agenda for these crops and their diseases for the next ten years. As such, it is an indispensable resource for plant pathologists and other crop scientists, policy makers, sociologists, and others involved in tropical or subtropical agriculture.

Sucking pests are most notorious group of pests for agricultural crops. Unlike most pests with chewing mouth parts, sucking pests cause more severe damage to the crops and are complex to get identified until advanced stages of infection. Not only is this late detection detrimental to their effective control, sucking pests also often cause fungal growth and virus transmission. The book emphasizes on sucking pests of most major crops of India. It aims to reflect Indian scenario before the international readership. This book complies comprehensive information on sucking pests of crops and brings the attention of the readers to this multiple damage causing insect complex. The chapters are contributed by highly experienced Indigenous experts from Universities & ICAR institutes, and book collates useful content for students and young researchers in plant pathology, entomology and agriculture.

Palms are monocots, Angiosperms, belonging to the family Palmae (Arecaceae), perennials having woody stems. Palmae (Arecaceae) family comprised of about six subfamilies, 200 genera and 2,700 species that are distributed all over the tropical, subtropical and Mediterranean landscape. Palms are diverse (ecologically and morphologically) group of plants. Ornamental palms are important component of landscape as well as interiorscapes. Additionally, these plants are good source of food, feed and shelter with numerous other commercial benefits. Likewise other trees and crops, landscape and field nurseries of palms are also subjected to various threats of insect pest and diseases (caused by different plant pathogens). Amongst fungal diseases leaf spots, leaf blights, Fusarium wilts, butt rots, bud rots, root rots, lethal yellowing and decline of palms are major growth constraints of palm growth. In developing countries very little attention has been paid on the etiology and management of these fungal diseases on ornamental palms. Accurate diagnosis and reliable management plan of palm fungal diseases usually requires expertise in both modern and advanced plant pathological approaches. Historically it was general belief that plant pathogens are not associated with human diseases. Since 19th century, several clinical reports are available indicating many plant pathogenic fungi (Aspergillus spp., Penicillium spp., Alternaria spp., Trichoderma spp., Fusarium spp., Curvularia spp. and Colletotrichum Spp) as novel agents of human diseases. Besides the association of fungal plant pathogens infecting ornamental palms, harbouring any of earlier mentioned or other fungal species (capable of causing certain diseases in human beings or pets) by the ornamental palms cultivation (either grown indoor or outdoor) is an important area of research to be explored and addressed thoroughly. This book will provide the deep information regarding major fungal diseases of ornamental palms, their symptoms, disease identification, and etiology and management strategies. This book will also provide unique knowledge regarding the ornamental palms harbouring kinds of human fungal pathogens and their practical management at domestic and commercial scale, in order to make cultivation of these plant more beneficial for humans, animals and environment.