This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phytoplasmas: Methods and Protocols aims to ensure successful results in the further study of this vital field.

It is difficult to forecast the serious consequences of damage to soil health on plants, animals and human beings. Agricultural soils across the globe are affected by erosion, degradation, nutrient depletion, pollution, organic matter decline and faulty management practices. This has resulted into 'Plant nutrient disorders' due primarily to imbalances, interactions, deficiencies or toxicities of one or more plant nutrients. Quality of irrigation water and environmental stress has a profound influence on this process. The present book provides an insight into the reasons for occurrence of such nutrient disorders, methodologies to monitor soil, plant, water and environment and ways to diagnose the problem through analytical and instrumental techniques. Widespread occurrence of secondary and micronutrient deficiencies in soils have resulted into low uptake of these nutrient ions by plants with adverse impact on human and animal health. Continuous cropping without due care of efficient soil and water use, have resulted in poor crop performances with low yields in many areas. This book attempts to provide answers to some pertinent questions on the subject i.e. I. Why plant nutrient disorders occur? II. Whether these are site or crop specific? III. How best one can diagnose the problem, by adopting sound analytical techniques? IV. What are the major concerns? V. How best one can manage such disorders to sustain soil health for crop production? Implementing scientific soil management programme requires good team work among scientists, extension workers & field staff with governmental support and farmer's participation. It is hoped that this book written in a simple language will be very useful for the students and teachers of Agriculture, Horticulture, Forestry, Fisheries & allied disciplines.

Examine the most recent developments in molecular plant pathology This comprehensive reference book describes the molecular biology of plant-pathogen interactions in depth. With Dr. Vidhyasekaran's keen insights and experienced critical viewpoint, Bacterial Disease Resistance in Plants: Molecular Biology and Biotechnological Applications not only presents reviews of current research but goes on to suggest future research strategies to exploit the studies in interventions with biotechnological, commercial, and field applications.This extraordinarily well-referenced book delivers in-depth examinations of: the molecular recognition process between plants and bacterial pathogens bacterial genes involved in the recognition process hrp, avr, dsp, and hsv genes the transcription of bacterial genes in plants signal transduction systems in bacteria and plants the functions of resistance genes and defense genes at the molecular level the elicitor molecules of bacterial pathogens and plants and their interactions plant and bacterial cell wall modifications and their role in triggering host defense mechanismsBacterial Disease Resistance in Plants also explores active oxygen species, inducible plant proteins and their signals and transcription mechanisms, inducible secondary metabolites, and more. It introduces novel strategies for bacterial disease management using genes from human beings, birds, crabs, insects, fungi, bacteria, and bacteriophages; and genetic engineering techniques that can be used to develop transgenic, disease-resistant plants.Generously illustrated with figures and tables that make the data more quickly understandable, Bacterial Disease Resistance in Plants will be an invaluable resource and textbook for plant pathologists, bacteriologists, botanists, plant physiologists, plant molecular biologists, microbiologists, biochemists, plant cell and applied biologists, genetic engineers, and graduate-level students in these disciplines.

Examine the most recent developments in molecular plant pathology This comprehensive reference book describes the molecular biology of plant-pathogen interactions in depth. With Dr. Vidhyasekaran's keen insights and experienced critical viewpoint, Bacterial Disease Resistance in Plants: Molecular Biology and Biotechnological Applications not only presents reviews of current research but goes on to suggest future research strategies to exploit the studies in interventions with biotechnological, commercial, and field applications.This extraordinarily well-referenced book delivers in-depth examinations of: the molecular recognition process between plants and bacterial pathogens bacterial genes involved in the recognition process hrp, avr, dsp, and hsv genes the transcription of bacterial genes in plants signal transduction systems in bacteria and plants the functions of resistance genes and defense genes at the molecular level the elicitor molecules of bacterial pathogens and plants and their interactions plant and bacterial cell wall modifications and their role in triggering host defense mechanismsBacterial Disease Resistance in Plants also explores active oxygen species, inducible plant proteins and their signals and transcription mechanisms, inducible secondary metabolites, and more. It introduces novel strategies for bacterial disease management using genes from human beings, birds, crabs, insects, fungi, bacteria, and bacteriophages; and genetic engineering techniques that can be used to develop transgenic, disease-resistant plants.Generously illustrated with figures and tables that make the data more quickly understandable, Bacterial Disease Resistance in Plants will be an invaluable resource and textbook for plant pathologists, bacteriologists, botanists, plant physiologists, plant molecular biologists, microbiologists, biochemists, plant cell and applied biologists, genetic engineers, and graduate-level students in these disciplines.

This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.

Proper identification of a problem is the prerequisite for devising the measures of its management as well as for applying the most suitable management strategy available. Plant diseases have always been an associated problem for successful crop cultivation and obtaining optimum profits from this most important enterprise. With advancements in the field of chemical management of plant diseases, we have witnessed a simultaneous increase in the problems related to pesticide application in agriculture. Part of this problem can be attributed to the improper identification of plant diseases, which leads to the use of improper chemical in injudicious amounts. It has, therefore, become necessary to aware the people involved in education, extension and practice of agriculture of proper characterization of plant diseases with the most appropriate management strategy for the disease. In this context, students, extension workers and farmers stand the major clients who must be armed with information on proper identification method and management of a given plant disease. The present book is designed to cater to the needs of undergraduate and postgraduate students of agriculture and biosciences, extension workers and educated progressive farmers. It is aimed to aid them in proper identification of many of the economically important plant diseases with integrated strategies for their respective management. The book may also serve as a guidebook for the professionals in the field of plant disease management for identification and management of plant diseases with minimal resource requirements

Physiological or abiotic disorders are mainly caused by changing environmental conditions such as temperature, moisture, unbalanced soil nutrients, inadequate or excess of certain soil minerals, extremes of soil pH and poor drainage. The distinction between physiological or abiotic disorders from other disorders is that they are not caused by living organisms (viruses, bacteria, fungi, insects, etc.), but they are the result of abiotic situations (inanimate) i.e. their agents are non-living in nature which causes deviation from normal growth. They results in physical or chemical changes in a plant which is far away from what is normal and is generally caused by an external factor. Non-infectious disorders in some cases are easy to identify, but others are difficult or even impossible to recognize. Most of them are non reversible once they have occurred. For the identification of physiological disorders it is important that one must know that: a) Physiological disorders are often caused by the deficiency or excess of something that supports life or by the presence of something that interferes with life. b) Physiological disorders can affect plants in all stages of their development. c) They are non-transmissible because they occur without or in absence of infectious agents. d) Plant reacts differently to the same agent and sometimes response is seen as a little reaction to death. e) Dealing with physiological disorders often means dealing with the consequences from a past event. f) Generally damaged and undamaged tissue is clearly demarcated. g) Physiological disorders not only causes damage themselves but also serve as the 'open door' (entry) for pathogens.

Vegetables are important source of dietary fibers, minerals, antioxidants and vitamins. Shifting from a non-vegetarian diet to vegetarian, global recognition of the importance of vegetables for human health and their medicinal and nutritional value have contributed to a steady upward trend in vegetable production system. China is ranked first in the world and currently produces around 237 million tons of vegetable. The total vegetable production of India during the year 2009-10 was approximately 90 million tons. Pesticides are valuable tools in sustainable vegetable production, but unfortunately they are often being used irresponsibly, causing hazards to producers, consumers and the environment. In addition, they can exacerbate pests and diseases problems by inducing resistance and suppressing the natural enemies which exert natural biological control. This book aims to promoting an integration of appropriate chemical, cultural, physical, genetic and biological technologies into Integrated Pest Management (IPM) strategies for vegetable crop protection.

This book has been written, primarily due to my own felt need to have a comprehensive text book on the pests of horticultural crops and methods to manage it, in the integrated way. Due to the widespread use of digital color photography and the good resolution that you get in even the basic level digital cameras, it is possible to get good photos. It was thus more of a need to have a text book to teach the UG and PG students, that this book was written. It was also necessary that any practicing field level worker like officers of the state department of Agriculture and commodity boards, and every practicing farmer with an ability to read English text book, should be guided into the basic facts about the pests of crops. It is most likely that most of the information is available in this book itself, as pest of some other crop. It will then be possible to read on the pest in that , and manage the pest effectively using the latest techniques available to the crop management expert.

The book is intended to provide a clear overview on the management of pests and diseases of horticulture crops, associated soil and beneficial fauna, residue status of pesticides and their estimation techniques. It is divided in four parts: Part I explain the practices followed in the pest management of horticulture crops. s include pest status of insects, mites, rodents, and diseases in fruits, vegetables, ornamentals, spices and mushrooms and their management. Different aspects of biological, cultural, and mechanical controls are also highlighted. Harmful and beneficial soil fauna associated with horticulture crops are dealt in Part II. Keeping in view the potential of beneficial organisms, the effects of pesticides on predators, parasites and pollinators have also been discussed in this section. The recent scientific developments related to residue status in vegetables, fruits and spices are provided in Part III. Part IV includes the residue estimation techniques of various pesticides.

Diagnosis involves careful observation of crop with a thorough knowledge of crop behaviour as well as a complete understanding of functions of nutrients and their deficiency symptoms. Diagnostic techniques vary from crop to crop depending upon the field condition in which the deficiency occurs. Indian agriculture has entered into an era of multiple nutrient deficiencies. Realizing the gravity of emerging problem of nutrient deficiencies and to fulfill an immediate need to tackle them more efficiently, a guide on diagnosis of nutritional disorders and their corrections in crop plants has been prepared. All the new diagnostic techniques have been discussed in a simple language. The book has been designed in such a way as to improve the knowledge on diagnosis of deficiencies of mineral elements essential for normal plant growth, and of the methods by which such deficiencies may most effectively be remedied. The main feature of this book is the detailed description of the various visual deficiency symptoms exhibited by the fruit crops. The book has been written primarily for the use of the students of Horticulture and Agriculture to help update their knowledge on nutritional and physiological disorders of fruit crops. It is also felt that the book will provide a suitable basis for those engaged in the profession of agriculture like extension workers and progressive farmers. We hope that this book will go a long way to help in increased fruit production by proper diagnosis and suitable correction of nutritional disorders in fruit crops.

The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations.
The Second Edition includes chapters on the following:
Sterilization of culture apparatus and culture media
Culture of pathogens with detailed techniques for 61 fungi and selected bacteria
Long-term storage of plant pathogens
Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens
Establishment of disease and testing for disease resistance
Work with soil microorganisms
Fungicide evaluation
Biological control
Bright-field microscopy

Hemp and cannabis, both belonging to Cannabis sativa, have emerged as some of the most valuable crops because of their multiple functionalities - industrial, medicinal, and recreational uses. Like all other crops, they are at risk of diseases and pests. In certain cases, an entire hemp field can fail due to unexpected disease. As a new and highly regulated crop, research on Cannabis crop diseases is scarce, and the science of plant diagnostics is not well covered in the literature. Taking hemp/cannabis as a model crop, the book illustrates how to diagnose a disease problem and how to manage it effectively. It presents real disease cases encountered during crop production, and explains methods of diagnosis, both in the field and in the lab, in order to find out the cause(s). The book provides: *A field and laboratory guide to diagnosing hemp and cannabis diseases and pest problems *Ready-to-adopt skills, methods and protocols in plant diagnosis, which can be applied to other crops *Over 300 colour photographs accompanied by a wealth of disease information, including field observations, unique symptoms, microscopic details, and molecular data. This book is essential for anyone who is interested in learning about Cannabis crop diseases, for crops grown in the field, and in indoor production facilities.

Plants have evolved both general and highly-specialized defense mechanisms that function to prevent diseases caused by the majority of microbial pathogens they encounter. Highly-specialized defense is governed by specific interactions between pathogen avr (avirulence) genes' loci and alleles of the corresponding plant disease resistance (R) loci. These defences can be very dynamic as microbes from the same species can act differently in their co-evolution with the specific host plant, which in turn has similarly evolved its response to external threats.There have been major developments in the field of plant-microbe interactions in recent years, due to newly developed techniques and the availability of genomic information. "Molecular Plant-Microbe Interactions" explores these new discoveries, focusing primarily on the mechanisms controlling plant disease resistance, the cross-talk among the pathways involved and the strategies used by the pathogens to suppress these defences. By exploring developments in plant defenses, pathogen's counter-defenses and mutually beneficial plant-microbe interactions, this book will be useful for researchers and students in plant pathology and plant biology-related areas.

Biological control, the management of pests by the use of living organisms, has a long history of application to agriculture around the world. However, the effective use of beneficial organisms is constrained by environmental, legal, and economic restrictions, forcing researchers to adopt increasingly multi-disciplinary techniques in order to deploy successful biological control programs. It is this complex process, including the mindset and the social environment of the researcher as well as the science being pursued, that this book seeks to capture. Chapters reveal the personal experiences of scientists from the initial search for suitable control agents, to their release into ecosystems and finally to the beneficial outcomes which demonstrate the great success of biological control across diverse agro-ecosystems.
Drawing together historical perspectives and approaches used in the development of biological control as well as outlining current debates surrounding terminology and differential techniques, Biological Control: A Global Perspective will be a valuable resource.

Toxic plants and other natural toxicants have a variety of roles in the fields of human health, medical research and the production of safe food and also represent an economic problem in terms of animal health and crop production. Estimates of economic impact on livestock have ranged in the millions of dollars in countries such as Australia and the United States. This book brings together applied and fundamental research from botanists, chemists, biochemists, agricultural scientists, veterinarians and physicians and advice from regulatory bodies. It consists of more than 100 edited papers from the Fifth International Symposium on Poisonous Plants, held in Texas in May 1997. All aspects of poisonous plants, mycotoxicoses and herbal intoxications are covered. Their adverse effects are described, such as fatalities, reduced or failed reproduction, fetotoxicity, spontaneous abortions, deformities, reduced productivity and organ-specific toxicity. Methods of detection, isolation and identification of the chemical compounds responsible are included. The biochemistry of the plant-associated toxins and elucidation of their mechanism of action is investigated, including the protocols for management or eradication, immunization programs, behaviour modification, withholding periods for metabolic detoxification, regulatory advice concerning human usage of natural products and advice concerning toxin-residue in agricultural produce. The development of non-toxic strains of plants for use as fodder is also discussed. This book is essential reading for toxicologists concerned with animal and human health, food industry regulators and plant scientists.

Genetics has transformed plant pathology on two occasions: first when Mendelian genetics enabled the discovery that disease resistance was a heritable trait in plants, and secondly when Flor proposed the "gene-for-gene" hypothesis to explain his observations of plant-parasite interactions, based on his work on flax rust in North Dakota starting in the 1930s. Our knowledge of the genetics of disease resistance and host-pathogen coevolution is now entering a new phase as a result of the cloning of the first resistance genes. This book provides a broad review of recent developments in this important and expanding subject. Both agricultural and natural host-pathogen situations are addressed. While most of the book focuses on plant pathology, in the usual sense of the term embracing fungal, bacterial and viral pathogens, there is also consideration of parasitic plants and a chapter demonstrating lessons to be learnt from the mammalian immune system. Three overall themes are addressed: genetic analyses and utilization of resistance; population genetics; and cell biology and molecular genetics. Chapters are based on papers presented at the British Society for Plant Pathology Presidential meeting held in December 1995, but all have been revised and updated to mid-1996. Written by leading authorities from North America, Europe and Australia, the book represents an essential update for workers in plant genetics, breeding, biotechnology and pathology.

This book provides extensive and comprehensive knowledge to the researchers/academics who are working in the field of cesium contaminated sites, and the impact on plants. This book is also helpful for graduate and undergraduate students who are specializing in radioecology or safe disposal of radioactive waste, remediation of legacies and the impact on the environment. Radiocesium (137Cs and 134Cs) was released into the environment as a result of nuclear weapons testing in 1950s and 1960s (~1x1018 Bq), and later due to the Chernobyl accident in 1986 (8.5x1016 Bq) and Fukushima Daiichi Nuclear Power Plant in 2011 (~1x1017 Bq). 137Cs is still of relevance due to its half-life of 30 years. The study of radioisotope 137Cs is important, as production and emission rates are high compared to other radioisotopes, due to high fission yield and high volatility. This book contains original work and reviews on how cesium is released into the environment on translocation from soil to plants and further on to animals and into the human food chain. Separate chapters focus on the effective half-life of cesium in plants and on how different cultivars are responding in accumulation of cesium. Other key chapters focus on cesium impact on single cells to higher plants and also on remediation measures as well as on basic mechanism used for remedial options and analysis of transfer factors. The book rounds off by contributions on cesium uptake and translocation and its toxicity in plants after the Chernobyl and Fukushima accidents.

This study, based on a literature review and simulations, shows the efficiency of cover crops at catching nitrate in most agriculture situations. It also analyzes both the negative impacts they can have and the ecosystem services they can provide. The introduction of a cover crop between two main crops helps catch the soil mineral nitrogen before the period of drainage and consequently reduces nitrate leaching and nitrate concentration in the drainage water. This study allows quantifying the efficiency of cover crops at catching nitrate and optimizing their implantation conditions over a large range of French pedoclimatic conditions. The presence of high nitrate levels in surface and ground waters, due to excessive nitrogen fertilization and natural production of nitrate by soil organic matter mineralization, is a double challenge for public health and environment protection.

This book summarizes our current knowledge on belowground defence strategies in plants by world-class scientists actively working in the area. The volume includes chapters covering belowground defence to main soil pathogens such as Fusarium, Rhizoctonia, Verticillium, Phytophthora, Pythium and Plasmodiophora, as well as to migratory and sedentary plant parasitic nematodes. In addition, the role of root exudates in belowground plant defence will be highlighted, as well as the crucial roles of pathogen effectors in overcoming root defences. Finally, accumulating evidence on how plants can differentiate beneficial soil microbes from the pathogenic ones will be covered as well. Better understanding of belowground defences can lead to the development of environmentally friendly plant protection strategies effective against soil-borne pathogens which cause substantial damage on many crop plants all over the world. The book will be a useful reference for plant pathologists, agronomists, plant molecular biologists as well as students working on these and related areas.

The knowledge of ecology and epidemiology of rhizomania is particularly useful to understand the means and practices able to limit or avoid its further diffusion. Some promising methods of biological control using coexisting and non-pathogenic organisms could potentially help improve the action of the not completely effective genetic resistances. This integrated protection would be valuable, especially in the even more frequent development of resistance-breaking strains in the BNYVV, where the known types of resistance, alone or in combination, seem to have lost part of their original ability to protect the crop. Therefore, further efforts will be needed to discover new traits likely still present in the wild species of the genus Beta. The availability of large collections of germplasm stored in the International Beta gene-banks should ensure the enhanced efficiency of genetic resistance by means of conventional and marker-assisted selection methods. Some almost immune transgenic varieties seem already to be waiting for release where and when it will be possible. The introduction chapter describes briefly the sugar beet crop, the more common diseases, and the damage caused by rhizomania. The following chapters discuss biological properties of the causal virus, BNYVV, and its vector, Polymyxa betae, and their interactions with the environment and the host-plant. In particular, the great advances in research of the molecular biology of BNYVV should be noteworthy, which have been established by a wide range of the most modern methods. Recent work focused on the genetic diversity and evolution of BNYVV is moving forward our understanding of the dramatic worldwide epidemics of rhizomania. Newly developed molecular techniques also lead to practical applications, such as quantification of inoculum in ecological and epidemiological research.

This book provides an overview of the current state of knowledge of the genetics and genomics of the agriculturally important Cucurbitaceae plant family, which includes crops such as watermelon, melon, cucumber, summer and winter squashes, pumpkins, and gourds. Recent years have resulted in tremendous increases in our knowledge of these species due to large scale genomic and transcriptomic studies and production of draft genomes for the four major species, Citrullus lanatus, Cucumis melo, Cucumis sativus, and Cucurbita spp. This text examines genetic resources and structural and functional genomics for each species group and across species groups. In addition, it explores genomic-informed understanding and commonalities in cucurbit biology with respect to vegetative growth, floral development and sex expression, fruit growth and development, and important fruit quality traits.

This book focuses on the evolution of plant viruses, their molecular classification, epidemics and management, covering topics relating to evolutionary mechanisms, viral ecology and emergence, appropriate analysis methods, and the role of evolution in taxonomy. The currently emerging virus species are increasingly becoming a threat to our way of life, both economically and physically. Plant viruses are particularly significant as they affect our food supply and are capable of rapidly spreading to new plant species. In basic research, plant viruses have become useful models to analyze the molecular biology of plant gene regulation and cell-cell communication. The small size of DNA genome of viruses possesses minimal coding capacity and replicates in the host cell nucleus with the help of host plant cellular machinery. Thus, studying virus cellular processes provides a good basis for explaining DNA replication, transcription, mRNA processing, protein expression and gene silencing in plants. A better understanding of these cellular processes will help us design antiviral strategies for plants. The book provides in-depth information on plant virus gene interactions with hosts, localization and expression and the latest advances in our understanding of plant virus evolution, their responses and crop improvement. Combining characterization of plant viruses and disease management and presenting them together makes it easy to compare all aspects of resistance, tolerance and management strategies. As such, it is a useful resource for molecular biologists and plant virologists alike.

This book describes the three gasotransmitters nitric oxide (NO), hydrogen sulphide (H2S) and carbon monoxide (CO) and their function as intracellular signalling molecules in plants. Common properties are shared by NO, H2S and CO: they are beneficial at low concentrations but hazardous in higher amounts; they are small molecules of gas; they can freely cross cell membranes; their effects do not rely on receptors; they are generated enzymatically and their production is regulated; their functions can be mimicked by exogenous application; and their cellular effects may or may not be mediated by second messengers, but have specific cellular and molecular targets. In plants, many aspects of the biology of gasotransmitters remain completely unknown and generate intriguing questions, which will be discussed in this book.

Handbook of Plant Disease Identification and Management presents the fundamentals of plant diseases identification based on symptomology and management focusing mainly on integrated pest management approach. It discusses a variety of techniques for the diagnosis of crop disease, losses due to crop diseases, and theories behind disease management. It describes how society is constraining the possibilities for management of crop diseases by changing the environment; biologically controlling crop diseases; and the epidemiologic and genetic concepts of managing host genes. This book discusses managing diseases through diverse chemical, biological, and physical methods. It highlights climatic factors affecting crops by creating favorable condition for most of the diseases. This book serves as a complete guide for growers, researchers, and graduate students to understand basics of plant disease identification. It explains the disease cycle for respective crops with favorable conditions promoting disease development. It intends to aid growers in managing diseases and help scientists with future research.