This book discusses and addresses the rapidly increasing world population demand for food, which is expected to double by 2050. To meet these demands farmers will need to improve crop productivity, which relies heavily on nitrogen (N) fertilization. Production of N fertilizers, however, consumes huge amounts of energy and the loss of excess N fertilizers to leaching results in the pollution of waterways and oceans. Therefore, increasing plant nitrogen use efficiency (NUE) is essential to help farmers produce more while conserving the environment. This book assembles some of the best work of top researchers from academic and industrial institutions in the area of NUE and provides valuable insight to scholars and researchers by its comprehensive discussion of current and future strategies to improve NUE through genetic manipulation. This book should also be highly valuable to policy makers, environmentalists, farmers, biotechnology executives, and to the hard-core researchers working in the lab.

This volume presents the issues and challenges of crop pathogens and plant protection. Composed of the latest knowledge in plant pathology, the book covers topics such as fungal diseases of the groundnut, plant growth promoting rhizobacteria, plant pathogenic fungi in the genomics era, the increased virulence of wheat rusts and oat fungal diseases.

Written by experienced and internationally recognized scientists in the field, "Future Challenges in Crop Protection Against Fungal Pathogens "is a concise yet comprehensive resource valuable for both novice as well as experienced plant scientists and researchers.

Plants are endowed with innate immune system, which acts as a surveillance system against possible attack by pathogens. Plant innate immune systems have high potential to fight against viral, bacterial, oomycete and fungal pathogens and protect the crop plants against wide range of diseases. However, the innate immune system is a sleeping system in unstressed healthy plants. Fast and strong activation of the plant immune responses aids the host plants to win the war against the pathogens. Plant hormone signaling systems including salicylate (SA), jasmonate (JA), ethylene (ET), abscisic acid (ABA), auxins, cytokinins, gibberellins and brassinosteroids signaling systems play a key role in activation of the sleeping immune systems. Suppression or induction of specific hormone signaling systems may result in disease development or disease resistance. Specific signaling pathway has to be activated to confer resistance against specific pathogen in a particular host. Two forms of induced resistance, systemic acquired resistance (SAR) and induced systemic resistance (ISR), have been recognized based on the induction of specific hormone signaling systems. Specific hormone signaling system determines the outcome of plant-pathogen interactions, culminating in disease development or disease resistance. Susceptibility or resistance against a particular pathogen is determined by the action of the signaling network. The disease outcome is often determined by complex network of interactions among multiple hormone signaling pathways. Manipulation of the complex hormone signaling systems and fine tuning the hormone signaling events would help in management of various crop diseases. The purpose of the book is to critically examine the potential methods to manipulate the multiple plant hormone signaling systems to aid the host plants to win the battle against pathogens.

This book provides a timely review of concepts in plant disease management involving microbial soil suppressiveness and organic amendments. Topics discussed include the impact of suppressive soils on plant pathogens and agricultural productivity, the enhancement of soil suppressiveness through the application of compost and the development of disease suppressive soils through agronomic management. Further chapters describe diseases caused by phytopathogens, such as Pythium, Fusarium and Rhizoctonia, interaction of rhizobia with soil suppressiveness factors, biocontrol of plant parasitic nematodes by fungi and soil suppressive microorganisms.

Allelopathy is an ecological phenomenon by which plants release organic chemicals (allelochemicals) into the environment influencing the growth and survival of other organisms. In this book, leading scientists in the field synthesize latest developments in allelopathy research with a special emphasis on its application in sustainable agriculture. The following topics are highlighted: Ecological implications, such as the role of allelopathy during the invasion of alien plant species; regional experiences with the application of allelopathy in agricultural systems and pest management; the use of microscopy for modeling allelopathy; allelopathy and abiotic stress tolerance; host allelopathy and arbuscular mycorrhizal fungi; allelopathic interaction with plant nutrition; and the molecular mechanisms of allelopathy. This book is an invaluable source of information for scientists, teachers and advanced students in the fields of plant physiology, agriculture, ecology, environmental sciences, and molecular biology.

This volume of "Advances in Virus Research "focuses on mycoviruses. The authors and reviews represent the most current and cutting-edge research in the field. A broad range of research is presented from research experts.
Key features:

* Contributions from leading authorities * Informs and updates on all the latest developments in the field

This book provides in-depth information on all key aspects of geminivirus biology, e.g. the genetics and evolution, global diversity and spread of these plant pathogens, as well as the molecular mechanisms underlying their virulence. Geminiviridae is one of the largest viral families, comprising numerous plant-infecting viruses that cause diseases in crops and weeds. These diseases have been reported from nearly all continents, in particular Asia, Europe, Africa and America. The book summarizes the current state of knowledge on the interactions between plant host and virus. In addition, it discusses advances regarding the trans-replication of satellite molecules and its effect on geminiviral pathogenesis, as well as pest management strategies to combat these diseases in the field. Given its scope, the book is a must-read reference guide for all researchers and advanced students working in virology, agriculture and plant biotechnology..

A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes. In Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens. These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

The theme of the book is highly relevant to the current emphasis on environment conservation, with focus on native biodiversity conservation in agro-ecosystems. The current impetus being given to organic farming and export oriented agri-hortculture in the country calls for access to relevant scientific knowledge base among the stakeholders. Research on biological pest control is more than a century old in India. Egg parasitoids, which are mainly tiny wasps, led by the family Trichogrammatidae, are the most widely utilized natural enemies for biological control globally. Over thirty countries are using these bioagents to protect over 10 million hectares of agricultural and forestry crops from many important insect pests. The book comprises 18 chapters, which are arranged in continuum, commencing with basic aspects of knowledge and ending in their utilization targets. The chapters cover broadly four areas: bio-diversity and natural occurrence of egg parasitoids, behaviour and adaptation of egg parasitoids, mass production and safe use of egg parasitoids and utilisation of egg parasitoids in different crop ecosystems. Some of the chapters cater to the needs of discipline-wise update on the current R&D scenario-like insect taxonomy, biotechnology, mass-production and quality control of the target organisms - egg-parasitoids, which are useful for laboratory scientists/researchers. There are also chapters devoted to knowledge status and scope for utilization of egg parasitoids in different target crops, which cater to requirements of field entomologists and extensionists for use in their tasks of guiding farmers/local guides. The book is different in approach, method, structure and content and ensures holistic coverage of the topic. The chapters are written by active and experienced workers in different crops and aspects and co-edited by four very experienced experts who have over three decades R&D experience in the subject. All the authors have uniformly focussed on comprehensive literature study and critical identification of knowledge gaps for future R&D, thus the book is novel in outlook, up-to-date in content and comprehensive in coverage of themes. This book will be useful for supplementary reading for MSc Agriculture and PhD Agriculture students, besides MSc/PhD research students in Zoology/Environmental Biology, who are specialising in Entomology. It would also serve as a very useful reference book for researchers worldwide, though focus is also there on Indian work. It addresses the special information needs of students and faculty, besides practitioners and extensionists in the Australasia and Africa regions and thus not limited to the R&D knowledge generated in developed countries.

This book describes how genomics has revolutionized our scientific understanding of agriculturally important plant-associated bacteria. Each chapter focuses on the genomics of particular bacteria: the first described plant pathogen, "Erwinia amylovora"; phytoplasmas lacking cell walls; fastidious, phloem-restricted liberibacters; "Pseudomonas syringae," which is a genetically tractable model system; "Xanthomonas citri," which causes a disease that can devastate citrus crops and "Pseudomonas fluorescens," which can protect plants from diseases.

Topics considered in this volume include the importance of horizontal gene transfer in originating new bacterial strains and species and advances in transcriptomics that allow us to describe the complex regulatory networks critical to plant-microbe interactions. The availability of the "Xanthomonas oryzae" genome has led to new technologies in genome editing, which will revolutionize approaches to genetic engineering, even in eukaryotes. The contributions show how genomics has greatly accelerated progress toward understanding the biology of these bacteria and how that understanding can be translated into novel crop protection methods.

Experience shows that biotic stresses occur with different levels of intensity in nearly all agricultural areas around the world. The occurrence of insects, weeds and diseases caused by fungi, bacteria or viruses may not be relevant in a specific year but they usually harm yield in most years. Global warming has shifted the paradigm of biotic stresses in most growing areas, especially in the tropical countries, sparking intense discussions in scientific forums. This book was written with the idea of collecting in a single publication the most recent advances and discoveries concerning breeding for biotic stresses, covering all major classes of biotic challenges to agriculture and food production. Accordingly, it presents the state-of-the-art in plant stresses caused by all microorganisms, weeds and insects and how to breed for them. Complementing Plant Breeding for Abiotic Stress Tolerance, this book was written for scientists and students interested in learning how to breed for biotic stress scenarios, allowing them to develop a greater understanding of the basic mechanisms of resistance to biotic stresses and develop resistant cultivars.

Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.

The current scenario of increasing sensitivity towards the sustainable agriculture has given a large space to extensively utilize natural resources that are environmental friendly and are a good replacement of chemicals in agriculture. Application of organic additives in the sustainable disease management can provide new insight in sustenance of plant productivity along with improved host stress tolerance. In the present book we have focussed upon a range of organic strategies to control plant pathogens of wide spectrum in addition to maintaining robust plant health. A detailed account on the application of organic additives has been discussed, irrespective of their origin and nature. In addition, the methods of utilising these organic supplements in the management of plant diseases and promotion of plant yield in more economic way have also been presented with reference to developing, underdeveloped and developed countries. The book has included the works of eminent scholars from across the world thus flashing light on the key literature related to application of organic matters including phytoextracts, chopped leaves, composted organic manures and liquid manures in eco-friendly agriculture. The mechanisms underlying the effectiveness of these organic amendments in promoting plant health has also been presented and discussed in understandable ways.

Precise regulation of gene expression in both time and space is vital to plant growth, development and adaptation to biotic and abiotic stress conditions. This is achieved by multiple mechanisms, with perhaps the most important control being exerted at the level of transcription. However, with the recent discovery of microRNAs another ubiquitous mode of gene regulation that occurs at the post-transcriptional level has been identified. MicroRNAs can silence gene expression by targeting complementary or partially complementary mRNAs for degradation or translational inhibition. Recent studies have revealed that microRNAs play fundamental roles in plant growth and development, as well as in adaptation to biotic and abiotic stresses. This book highlights the roles of individual miRNAs that control and regulate diverse aspects of plant processes.

Our lives and well being intimately depend on the exploitation of the plant genetic resources available to our breeding programs. Therefore, more extensive exploration and effective exploitation of plant genetic resources are essential prerequisites for the release of improved cultivars. Accordingly, the remarkable progress in genomics approaches and more recently in sequencing and bioinformatics offers unprecedented opportunities for mining germplasm collections, mapping and cloning loci of interest, identifying novel alleles and deploying them for breeding purposes. This book collects 48 highly interdisciplinary articles describing how genomics improves our capacity to characterize and harness natural and artificially induced variation in order to boost crop productivity and provide consumers with high-quality food. This book will be an invaluable reference for all those interested in managing, mining and harnessing the genetic richness of plant genetic resources.

Microbial Phenazines: Biosynthesis, Agriculture and Health focuses on phenazines, a group of upwards of a hundred nitrogen-containing redox-active heterocyclic compounds of bacterial origin that have long attracted scientific interest because of their colorful pigmentation. Our understanding of these fascinating natural products and their role in human health and the environment has advanced rapidly in recent years, but we are only now beginning to be able to exploit the potential of these compounds in such fields as agriculture and medicine. This volume includes information on the biochemistry and genetics of phenazine synthesis, the physiological effects of phenazines, and methods for the isolation and identification of phenazines with the aid of spectroscopic and electrophoretic techniques. Also included are chapters focused on the roots of phenazine research in the biological control of plant pathogens and recent knowledge of the diversity of phenazine-producing microorganisms and the environments in which they occur. A final chapter addresses the potential of phenazines in the treatment of cancer.

Viruses are a huge threat to agriculture. In the past, viruses used to be controlled using conventional methods such as crop rotation and destruction of the infected plants but now there are more novel ways to control them. This volume focuses on natural and engineered virus resistance, the two major strategies used for crop protection.

* Contributions from leading authorities * Informs and updates on all the latest developments in the field

This book provides biological and agricultural insights into snow mold, a fungal disease affecting land plants observed after the melting of snow. Snow mold fungi can cause significant damage to plant growth both in agriculture and in the natural environment, but the interesting ecology and biology described here will capture the attention of scientists in diverse disciplines. The book describes diverse biological phenomena such as cold tolerance of snow mold fungi and plants and their interactions, occurring in an ecologically unique environment under the snow, which maintains constant low temperature and high humidity. Presented here are the unique strategies of snow mold fungi to survive in diverse habitats and the defensive mechanism in plants tolerant to snow mold fungi infection, as well as the conventional control methods using fungicide or cultural practice. Also contained in the book is speculation on the impact of a changing environment on snow mold diseases and their effects on agricultural production.

Petunia belongs to the family of the Solanaceae and is closely related to important crop species such as tomato, potato, eggplant, pepper and tobacco. With around 35 species described it is one of the smaller genera and among those there are two groups of species that make up the majority of them: the purple flowered P.integrifolia group and the white flowered P.axillaris group. It is assumed that interspecific hybrids between members of these two groups have laid the foundation for the huge variation in cultivars as selected from the 1830 s onwards.

Petunia thus has been a commercially important ornamental since the early days of horticulture. Despite that, Petunia was in use as a research model only parsimoniously until the late fifties of the last century. By then seed companies started to fund academic research, initially with the main aim to develop new color varieties. Besides a moment of glory around 1980 (being elected a promising model system, just prior to the Arabidopsis boom), Petunia has long been a system in the shadow. Up to the early eighties no more then five groups developed classical and biochemical genetics, almost exclusively on flower color genes. Then from the early eighties onward, interest has slowly been growing and nowadays some 20-25 academic groups around the world are using Petunia as their main model system for a variety of research purposes, while a number of smaller and larger companies are developing further new varieties.

At present the system is gaining credibility for a number of reasons, a very important one being that it is now generally realized that only comparative biology will reveal the real roots of evolutionary development of processes like pollination syndromes, floral development, scent emission, seed survival strategies and the like.

As a system to work with, Petunia combines advantages from several other model species: it is easy to grow, sets abundant seeds, while self- and cross pollination is easy; its lifecycle is four months from seed to seed; plants can be grown very densely, in 1 cm2 plugs and can be rescued easily upon flowering, which makes even huge selection plots easy to handle. Its flowers (and indeed leaves) are relatively large and thus obtaining biochemical samples is no problem. Moreover, transformation and regeneration from leaf disc or protoplast are long established and easy-to-perform procedures. On top of this easiness in culture, Petunia harbors an endogenous, very active transposable element system, which is being used to great advantage in both forward and reverse genetics screens.

The virtues of Petunia as a model system have only partly been highlighted. In a first monograph, edited by K. Sink and published in 1984, the emphasis was mainly on taxonomy, morphology, classical and biochemical genetics, cytogenetics, physiology and a number of topical subjects. At that time, little molecular data was available. Taking into account that that first monograph will be offered electronically as a supplement in this upcoming edition, we would like to put the overall emphasis for the second edition on molecular developments and on comparative issues.

To this end we propose the underneath set up, where chapters will be brief and topical. Each chapter will present the historical setting of its subject, the comparison with other systems (if available) and the unique progress as made in Petunia. We expect that the second edition of the Petunia monograph will draw a broad readership both in academia and industry and hope that it will contribute to a further expansion in research on this wonderful Solanaceae."

The Grasses and Native Plants manual is a reference manual on diseases which attack grasses, forage, native flowers, and weeds. The manual identifies various types of diseases which are known to invade these plants located throughout North, Central, and South America. The recordings include diseases caused by fungi, bacteria, viruses, viroids, phytoplasmas, and nematodes. Causal disease agents are described and illustrated in some cases and diseases and disease control measures are also discussed. A manual such as this is never finished since new reports of diseases are continuously reported.

The book illustrates the use of putative microbial agents which provide good protection to the plant from biotic pathogens attack. An up to date knowledge on plant-microbiome interaction strategies in terms of improved sustainability has been discussed. Information from experts across the globe on the application of microbes for providing amicable solution in sustainable agriculture has been gathered. In addition, information related to microbes mediated resistance levels leading to enhanced plant health has been well presented. The chapters have emphasised the use of Plant Growth Promoting Rhizobacteria (PGPR) and other potential biocontrol agents/antagonists in the management of plant diseases which provide extensive information to the readers. Literature on microbial root colonization, plant growth promotions, and also on the protection of plants from attack of various soil borne pathogens have been presented in a coherent way. Information on the application of potential strain of the bio-control fungi, endophytes, actinomycetes strengthening the plants ability which rescue the plant from pathogens attack leading to improved plant health has also been underpinned.

In 1992 a Concerted Action Programme (CAP) was initiated by Peter Sijmons with the purpose of intensifying collaborations between 16 European laboratories working on plant-parasitic nematodes. The four-year programme entitled "Resistance Mechanisms Against Plant-Parasitic Nematodes" focused on molecular aspects of the interaction between sedentary nematodes and plants on the model system Arabidopsis and on novel resistance strategies. Funding was provided mainly for exchange visits between collaborating laboratories and for the organization of annual meetings. During the last annual meeting which was held in May 1996 in Toledo, Spain, Carmen Fenoll initiated the production of this volume. The text presents a series of current reviews, each written by one of the participating laboratories, which include the scientific progress achieved in the frame of this CAP but are by no means limited in scope to this work.

This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community.