This book is based on papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. Since the first symposium (published as Acta Horticulturae 225, 1988), there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centered on attempts to separate, spatially, the problems of vertical disease transmission, via infected explants to progeny microplants, from laboratory contamination. Disease control is best achieved by establishing pathogen-free cultures, while laboratory contamination management is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most important causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality micro-propagules. In this book, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo. The contents are divided into sections. In each section there are papers that update contributions offered at the first symposium; for example, major advances have been made in microbial taxonomy and diagnosticsbased on advances in DNA-based techniques. Consolidation has occurred in therapy and laboratory contamination management. Novel, and arguably speculative, in vitro contamination control based on autotrophic culture is described; and disease control at establishment using bacterial and mycorrhizal inoculants is discussed.

In response to low iron availability in the environment most microorganisms synthesize iron chelators, called siderophores. Bacteria and fungi produce a broad range of structurally diverse siderophores, which all show a very high affinity for ferric ions.

"Microbial Siderophores" presents an up-to-date overview of the chemistry, biology and biotechnology of these iron chelators. Following an introduction to the structure, functions and regulation of fungal siderophores, several chapters focus on siderophores of pseudomonads. Here, the technique of siderotyping, which has proved to be a rapid, accurate and inexpensive tool for pseudomonad characterization and identification, is described. Further, the biological significance of siderophores of symbiotic fungi and the possible role of siderophores in pathogenesis are discussed. In addition to methodological approaches, chapters on the biotechnological production of siderophores and their application in promoting human and plant health are included.

Plants use resources, i.e. carbon, nutrients, water and energy, either for growth or to defend themselves from biotic and abiotic stresses. This volume provides a timely understanding of resource allocation and its regulation in plants, linking the molecular with biochemical and physiological-level processes. Ecological scenarios covered include competitors, pathogens, herbivores, mycorrhizae, soil microorganisms, carbon dioxide/ozone regimes, nitrogen and light availabilities. The validity of the Growth-Differentiation Balance Hypothesis is examined and novel theoretical concepts and approaches to modelling plant resource allocation are discussed. The results presented can be applied in plant breeding and engineering, as well as in resource-efficient stand management in agriculture and forestry.

"

For investigators engaged in the study of toxins generally, and host-specific toxins in particular, it is a rare treat to attend a meeting in which toxins involved in plant pathogenesis are emphasized. A gathering of this type provides opportunity to consider the discovery of new toxins, their chemical structures, genes encoding enzymes that control their biosyntheses, their sites of action and physiological effects on plants, and their roles (if any) in pathological processes. Having acknowledged the inspiration fostered by a 'toxin meeting', however, it is important to point out that the program of this symposium was generously sprinkled with 'nontoxin' talks. These contributions generated cross-disciplinary discussion and promoted new ways of thinking about relationships among factors required for plant disease development. The point can be illustrated by considering just one example. We have in the past often regarded diseases mediated by host-specific toxins and diseases involving 'gene-for-gene' relationships as representing two different classes of fungal/plant interaction. This is largely because the key molecular recognition event in so-called 'toxin' diseases leads to compatibility, whereas the corresponding event in 'gene-for-gene' diseases leads to incompatibility. Yet the race specific elicitors produced by the 'gene-for-gene' fungi Cladosporium fulvum (De Wit, Adv. Bot. Res. 21:147- 185, 1995) and Rhynchosporium secalis (Rohe et a1. , EMBO J.

Fundamentals of Plant Virology is an introductory student text covering all of modern plant virology. The author, Dr. R.E.F. Matthews, has written this coursebook based on his classic and comprehensive Plant Virology, Third Edition. Four introductory chapters review properties of viruses and cells and techniques used in their study. Five chapters are devoted to current knowledge of all major plant viruses and related pathogens. Seven chapters describe biological properties such as transmission, host response, disease, ecology, control, classification, and evolution of plant viruses. A historical and future overview concludes the text. Fundamentals of Plant Virology is a carefully designed instructional format for a plant virology course. It is also an invaluable resource for students of plant pathology and plant molecular biology.
Key Features
* Summarizes knowledge on all aspects of plant virology
* Condenses all essential material from Plant Virology 3/e
* Compares basic properties of cells and viruses
* Outlines principles of gene manipulation technology
* Discusses serological techniques including monoclonal antibodies
* Geared to student level course

''Informative, well-constructed, and readable...The contributors are leaders in their fields and what they have to say is worthwhile.'' --- SGM Quarterly, August 1998

Pine forests face a global threat of pine wilt disease, which is being spread by vector beetles carrying pathogenic nematodes from dead trees to healthy ones. Among the host pines there are varying degrees of susceptibility, and nematode strains also contain a variety of virulences, both of which factors help to determine whether infected host trees will die or survive. As well, biotic and abiotic environmental factors influence the fate of infected trees. This book describes the history of the disease, pathogenic nematodes, vector beetles, the etiology and ecology of the disease, microorganisms involved, and control methods that utilize host resistance and biological control agents. Concrete, comprehensive, and the most up-to-date knowledge about this worldwide forest epidemic is presented for readers, enabling them to understand the nature and epidemic threat of pine wilt disease.

Agricultural research is one of the most widespread forms of organized research in the world, in both developed and developing countries. Management of agricultural research involves many decisions that have scientific, social and political consequences. Every country has established agricultural research priorities based on many complex factors that must be considered when decisions are made on the choice of research problems to be investigated. Resources must be divided among projects that often compete for the limited funding available that supports the total research enterprise. This book will provide those individuals who manage research with methods and procedures that can be used in their management organization.

Westcott's Plant Disease is a reference book on diseases which attack plants. Diseases of plants are found on most all plants including trees, shrubs, grasses, forage, fruits, vegetables, garden and greenhouse plants as well as native wild flowers and even weeds. Plant Disease Handbook identifies various types of diseases which are known to invade these plants located throughout North and South America. The recordings include diseases caused by fungi, bacteria, viruses, viroids and nematodes. Causal disease agents are described and illustrated in many cases and diseases and disease control measures are also discussed. A book such as this is never finished since new reports of diseases are continuously reported. This includes new diseases and previously known diseases which occur on both presently recorded plants and on new plants found to be susceptible to diseases. Westcott's Plant Disease provides a reference and guide for identification and control of these plant disease problems.

This volume contains the Proceedings of the International Conference on Effects of Crop Rotation on Potato Production in the Temperate Zones. This conference was held in the International Agricultural Centre at Wageningen, The Netherlands, August 14 - 19, 1988, under the auspices of the European Association for Potato Research (EAPR), the Potato Association of America (PAA) and the Dutch National Council for Agricultural Research (NRLO). The length of rotations affect the performance of potato crops and yields are often reduced if rotations are too short. The kinds of crops within a rotation will also affect potato performance, especially those grown in the previous season. It was a major aim of the conference to bring together specialists of various disciplines to examine these determinants of potato yield. The Organizing Committee is glad that all relevant aspects of the theme are highlighted in these proceedings. The contents of the contributions are primarily left to the responsibility of the authors. J. Vos C.D. van Loon G.J. Bollen ORGANIZATION OF THE CONFERENCE The European Association for Potato Research (EAPR) and the Potato Association of America (PAA) nominated representatives in the Conference Committee. PAA was represented by prof. dr E.E. Ewing (Ithaca, USA) and by dr R.E. Thornton (Pullman, USA); EAPR was represented by prof. dr J.C. Holmes (Edinburgh, UK), ir C.D. van Loon (Lelystad, NL), and dr ir J. Vos (chairman; Wageningen, NL).

This book aims to assess, evaluate and critically analyze the methods that are currently available for a judicious pest management in durable food. It presents and analyzes a vast amount of methods that are already in use in "real world" industrial applications. After the phase-out of methyl bromide, but also the withdrawal of several insecticides and the continuously updated food safety regulations, there is a significant knowledge gap on the use of risk-reduced, ecologically-compatible control methods that can be used with success against stored-product insect species and related arthropods. The importance of integrated pest management (IPM) is growing, but the concept as practiced for stored products might differ from IPM as historically developed for field crops. This book discusses a wide variety of control strategies used for stored product management and describes some of the IPM components. The editors included chemical and non-chemical methods, as both are essential in IPM. They set the scene for more information regarding emerging issues in stored product protection, such as emerging, alien and invasive species as threats for global food security, as well as the importance of stored-product arthropods for human health. Finally, the analysis of the economics of stored product protection is presented, from theory to practice.

This volume addresses the similarities and also the differences in the genomes of soil saprophytes, symbionts, and plant pathogens by using examples of fungal species to illustrate particular principles. It analyzes how the specific interactions with the hosts and the influence of the environment may have shaped genome evolution. The relevance of fungal genetic research and biotechnological applications is shown for areas such as plant pathogenesis, biomass degradation, litter decomposition, nitrogen assimilation, antibiotic production, mycoparasitism, energy, ecology, and also for soil fungi turning to human pathogens. In addition to the model organisms Neurospora and Aspergillus, the following species are covered providing a view of pathogens and mutualists: Trichoderma, Fusarium oxysporum, Cochliobolus heterostrophus, Penicillium chrysogenum, Rhizopus oryzae, Podospora anserina, and species belonging to Agaricomycetes, Archaeorhizomycetes and Magnaporthaceae. Ecology and potential applications have guided the choice of fungal genes to be studied and it will be fascinating to follow the trends of future sequencing projects.

Microorganisms, including those genetically modified (GMOs), are increasingly being released deliberately into the environment for different purposes. One major interest is the use of antagonistic organisms for biological control of pests and plant diseases. Both in the development of biocontrol agents and their later risk assessment and product approval, there is a need for methods that facilitate monitoring of such introduced microorganisms. This book particularly addresses the various approaches for monitoring introduced fungi and the current status of techniques which may be relevant to use are described. Such techniques include marker genes and other DNA-based techniques, serology using polyclonals or monoclonals, the use of enzymes or secondary metabolite profiles, and electronic noses' as well as more traditional methods like dilution plating.

The future of agriculture greatly depends on our ability to enhance productivity without sacrificing long-term production potential. The application of microorganisms, such as the diverse bacterial species of plant growth promoting bacteria (PGPB), represents an ecologically and economically sustainable strategy. The use of these bio-resources for the enhancement of crop productivity is gaining importance worldwide. "Bacteria in Agrobiology: Disease Management" discusses various aspects of biological control and disease suppression using bacteria. Topics covered include: fluorescent pseudomonads; siderophore-producing PGPR; pseudomonas inoculants; bacillus-based biocontrol agents; bacterial control of root and tuber crop diseases; fungal pathogens of cereals; soil-borne fungal pathogens; peronosporomycete phytopathogens; and plant parasitic nematodes.

In 1992 a Concerted Action Programme (CAP) was initiated by Peter Sijmons with the purpose of intensifying collaborations between 16 European laboratories working on plant-parasitic nematodes. The four-year programme entitled "Resistance Mechanisms Against Plant-Parasitic Nematodes" focused on molecular aspects of the interaction between sedentary nematodes and plants on the model system Arabidopsis and on novel resistance strategies. Funding was provided mainly for exchange visits between collaborating laboratories and for the organization of annual meetings. During the last annual meeting which was held in May 1996 in Toledo, Spain, Carmen Fenoll initiated the production of this volume. The text presents a series of current reviews, each written by one of the participating laboratories, which include the scientific progress achieved in the frame of this CAP but are by no means limited in scope to this work.

Emphasis in this second volume of Advances in Downy Mildew Research is on the biology of compatible interactions, forecasting and epidemiology, host specialisation, genetic variability amongst pathogen populations, novel methods for detection and systematics, and induced resistance. Two chapters focus on the related oomycete Albugo candida, which shares many pathogenic characteristics with the downy mildews and provides a valuable comparative pathosystem. Contributions on specific downy mildews include Bremia lactucae, Peronospora destructor, Peronospora sparsa, Peronospora viciae, Plasmopara halstedii, Plasmopara viticola, Pseudoperonospora cubensis and Sclerospora graminicola. Review chapters on compatibility, forecasting and systematics consider a broader range of downy mildew fungi, and compare them with other oomycete and biotrophic pathogens. The book is relevant to anyone with an interest in these unique biotrophic pathogens, either in their own right as causes of damaging diseases or as model systems for research on host-pathogen interactions. It should be read by: students, teachers and researchers in academic and research institutes; research and development personnel in the agrochemical industries; agricultural and horticultural advisers, and other extension workers.

OY (])PONEOYLI TOIA YTA IIOAAOI OKOEOJII Many fail to grasp what they have seen, and cannot judge what they have learned, ErKYPEOYLI OYL1E MA (R)ONTEE ITINOEKOYIT although they tell themselves they know. EQYTOJII L1E L10KEOYLI Heraclitus of Ephesus, 500 BC " ... everyone that is not speckled and C~T~v: N,;~, 'T,ji~ ,~~~N ,tuN '= spotted among the goats and brown among the sheep, that shall be counted ~~N N,n =,~, c~w=== c,m stolen with me." Genesis Chapter 30 From Heraclitus of Ephesus and later philosophers, we can deduce that observation of natural phenomena, even when keen and accurate, will not result in meaningful knowledge unless combined with analysis of the mind; just as analysis of the mind without acquaintance with natural phenomena will not suffice to grasp the perceivable world. Only familiarity with phenomena combined with mental analysis will lead to additional knowledge. The citation from Genesis, Chapter 30, is part of an unusual story. It tells how Jacob received, as payment for his service to Laban, the bulk of Laban's herds. By agreement, Jacob was to receive "only" the newborn speckled and spotted goats and the newborn brown sheep that differed completely from their parents. Did Jacob know that there was instability (transposable elements?) in the pigmentation of Laban's herd? It is reasonable to assume that Jacob combined his keen observation with analysis of his mind in order to predict the outcome: most of the newborns were indeed speckled, spotted or brown.

Trichodorids constitute a group of nematodes, together with tylenchs and longidorids, that are parasitic on plants. The trichodorids form the smallest group in a number of genera, species and also individuals in the soil. However, they are not at all devoid of interest on both economical and zoological grounds, and they are being studied with increasing attention by agronomists and taxonomists. Trichodorids are important in strictly agronomical research, as they are able to cause direct damage to the root system of various crops; they are the agent of the docking disorder' of the sugar beet, a severe disease in Europe. They are also able to transmit various viruses to a series of important crops. These viruses differ from those transmitted by the longidorids and also - as opposed to longidorids - they are not strictly associated with one or a restricted number of species. Trichodorids therefore constitute a potentially active group of direct and indirect predators. The present book has been written by one of the leading specialists on the group, who has produced an up to date review of data on the morpho-anatomy of all species of the group. Moreover, the book is not, as is so often the case, based merely on a literature survey, but on the author's personal observations. The descriptions are supported by detailed drawings and micrographs and in many instances new characters of taxonomic significance have been identified. Attention to detail and keen observation have enabled the author to provide a considered and authoritative opinion on the taxonomy and systematics of the Trichodoridae. Further, the evaluation and selection of specific characteristics clearly demonstrate the importanceof reliable identification of species when undertaking investigations on geographical distribution, ecology and other facets of the biology of individual species, particularly those of economic importance. It is inevitable that in such an actively investigated taxon as the Trichodoridae, there will be a continuing addition of new species which in turn will encourage further expression of taxonomic opinion. If that proves to be so, Dr. Decraemer's book will provide a well-established foundation for any new opinion and will remain a standard reference for Trichodoridae as well as a substantial contribution to the science of nematology. Audience: Research workers and students of agriculture, horticulture and zoology, plant pathologists and extension nematology service.

The book provides a fascinating overview about current and sophisticated developments in applied entomology that are powered by molecular biology and that can be summarized under a novel term: insect biotechnology. By analogy with the application of powerful molecular biological tools in medicine (red biotechnology), plant protection (green biotechnology) and industrial processing (white biotechnology), insect biotechnology (yellow biotechnology) provides novel tools and strategies for human welfare and nutrition. Insect Biotechnology has emerged as a prospering discipline with considerable economic potential, and encompasses the use of insect model organisms and insect-derived molecules in medical research as well as in modern plant protection measures.

This book provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts. This will help to develop better methods for disease control in plants and animals.

This book provides extensive and comprehensive knowledge to the researchers/academics who are working in the field of cesium contaminated sites, and the impact on plants. This book is also helpful for graduate and undergraduate students who are specializing in radioecology or safe disposal of radioactive waste, remediation of legacies and the impact on the environment. Radiocesium (137Cs and 134Cs) was released into the environment as a result of nuclear weapons testing in 1950s and 1960s (~1x1018 Bq), and later due to the Chernobyl accident in 1986 (8.5x1016 Bq) and Fukushima Daiichi Nuclear Power Plant in 2011 (~1x1017 Bq). 137Cs is still of relevance due to its half-life of 30 years. The study of radioisotope 137Cs is important, as production and emission rates are high compared to other radioisotopes, due to high fission yield and high volatility. This book contains original work and reviews on how cesium is released into the environment on translocation from soil to plants and further on to animals and into the human food chain. Separate chapters focus on the effective half-life of cesium in plants and on how different cultivars are responding in accumulation of cesium. Other key chapters focus on cesium impact on single cells to higher plants and also on remediation measures as well as on basic mechanism used for remedial options and analysis of transfer factors. The book rounds off by contributions on cesium uptake and translocation and its toxicity in plants after the Chernobyl and Fukushima accidents.

Genetics and Genomics of Populus provides an indepth description of the genetic and genomic tools and approaches for Populus, examines the biology that has been elucidated using genomics, and looks to the future of this unique model plant. This volume is designed to serve both experienced Populus researchers and newcomers to the field. Contributors to the volume are a blend of researchers, some who have spent most of their research career on Populus and others that have moved to Populus from other model systems. Research on Populus forms a useful complement to research on Arabidopsis. In fact, many plant species found in nature are - in terms of the life history and genetics - more similar to Populus than to Arabidopsis. Thus, the genetic and genomic strategies and tools developed by the Populus community, and showcased in this volume, will hopefully provide inspiration for researchers working in other, less well developed, systems.

This book systematically presents 40 pests, 7 natural pest enemies, and 20 diseases and weeds commonly encountered in sugarcane production, combining clear colour photos with detailed scientific descriptions. It covers a range of related topics, including morphological identification, habits and frequency of occurrence, prevention and control measures, symptom identification, characteristics of infections and epidemics, parasitic (predator) characteristics, ways of utilising natural pest enemies, main species and distribution, fluctuation in the field, and chemical control of weeds. With novel content presented in simple, straightforward language, the book provides a valuable reference guide for scientific researchers, educators and industrial practitioners, as well as students and advisers at agricultural universities.

Phytoplasma-associated diseases are a major limiting factor to quality and productivity of many ornamentals, horticultural and other economically important agriculture crops worldwide. Annual losses due to phytoplasma diseases in many crops vary, but under the pathogen favorable conditions they always lead to disastrous consequences to farming community. As there is no effective cure for phytoplasma diseases, the management options emphasize on their exclusion, minimizing their spread by insect vectors and propagation materials and on development of host plant resistance. The phytoplasma associated plant diseases have a history of more than 50 years. Phytoplasmas have undoubtedly infected plants and cause diseases for centuries before they are described and proven to be the causal agents. But important progress related to identification of phytoplasmas only began after 1980's. Phytoplasmas have emerged as the most serious constraints in the production of several crops all around the world during last four decades. Phytoplasmas constitute a major limiting factor to quality and productivity of cereals, horticultural, ornamentals and many other economically important crops all over the world. Annual losses due to phytoplasma diseases may vary, but under the pathogen favorable condition, phytoplasma disease may lead to disastrous consequences for farming and industry community. The scientific literature concerning phytoplasma occurrence, characterization, diagnosis, detection, and management is growing at a fast pace. Significant advancement in the last decades on diagnostic, biological and molecular properties, epidemiology, host-pathogen-insect interactions as well as management of phytoplasmas has been made. Till date, no authentic compilation is available to know the progress of phytoplasmas characterization major crops all over the world. The planned book will compile all the updated information available information on phytoplasmas by distinguished experts in the form of edited book entitled "Characterization and epidemiology of phytoplasma associated diseases". The book covers recent and update information on emerging and re-emerging phytoplasma diseases affecting important crops in tropics and subtropics. It provides comprehensive information on disease distribution, occurrence, and identification of the phytoplasmas including the recent approaches for diagnostics, transmission, and information about losses and geographical distribution along with and management aspects. This volume contains 11-12 chapters contributed by the experienced and recognized experts working on different group of phytoplasmas affecting major crops all over the world. The information on various topics is at advanced as well as comprehensive level and provides the period wise developments of phytoplasma research. The book covers major chapters on an up to date progress of phytoplasma research, and then phytoplasma diseases associated with vegetable, pulse, oils crops, cereals, sugar crops, fruit crops, ornamentals, medicinal plants, palms species, forest tress and weeds. We have covered historical background, geographical distribution, identification and characterization, genetic diversity, host pathogen interaction and management aspects of important phytoplasma diseases infecting our major agricultural crops. The information on various topics is advanced as well as comprehensive, and provides thought provoking ideas for planning novel research ideas for future. This book will be useful to everyone interested in mollicutes, phytoplasma, spiroplasmas, plant pathology, disease control and plant biology and serve as an exhaustive and up-to-date compendium of references on various aspects of different groups of phytoplasmas affecting important crops worldwide.

Advances in Rice Blast Research provides a complete overview of the research undertaken on the rice-blast pathosystem. This book gathers in one volume the most recent works on rice blast fungus genetics and molecular biology of pathogenicity, rice blast fungus population studies, and genetics and molecular biology of rice resistance to blast, including resistance gene cloning. It also presents the latest results on resistance breeding and resistance management strategies, epidemiology and disease management. This book is a must for plant pathologists and breeders working on rice blast and also to plant pathologists and breeders dealing with fungal diseases in general, because the rice-blast pathosystem is a model in plant pathology. Advances in Rice Blast Research provides a complete overview of the research undertaken on the rice-blast pathosystem. This book gathers in one volume the most recent works on rice blast fungus genetics and molecular biology of pathogenicity, rice blast fungus population studies, and genetics and molecular biology of rice resistance to blast, including resistance gene cloning. It also presents the latest results on resistance breeding and resistance management strategies, epidemiology and disease management. This book is a must for plant pathologists and breeders working on rice blast and also to plant pathologists and breeders dealing with fungal diseases in general, because the rice-blast pathosystem is a model in plant pathology.