The fifth and last Volume of this IPMD series reviews, in a multi-disciplinary approach, recent achievements in crop protection and integrated management of arthropod pests. The volume is organized in a first Section covering IPM in citrus productions, a Second one dealing with advacements in the integratioon of management technologies and a last Section covering mites and their biological control agents. As for the previous volumes, we attempted to provide an informative coverage for a broad range of agricultural systems and situations. The chapters are mainly organized and centered on crops, with a particular emphasis on citrus. This is one of the main crops in which biological control and IPM approaches were tested successfully, and the experience gained herein may indeed result helpful for IMP efforts deployed worldwide on other crops and/or cropping systems. Chapters in Section 2 review the integration of insect and disease management options in pecan crops, the application of remote sensing technologies, the status of knowledge about plant defense compounds and their potentials. For IPM of invasive species, an update is provided on the experience gained on the Red Palm Weevil (RPW) in Egypt. Long-term technological solutions for IPM in forests and park areas through aerial treatments with Bt spores concludes this Section. Finally, in Section 3, updated reviews about biological control agents of mites are provided.

Mycorrhizal fungi are microbial engines which improve plant vigor and soil quality. They play a crucial role in plant nutrient uptake, water relations, ecosystem establishment, plant diversity, and the productivity of plants. Scientific research involves multidisciplinary approaches to understand the adaptation of mycorrhizae to the rhizosphere, mechanism of root colonization, effect on plant physiology and growth, biofertilization, plant resistance and biocontrol of plant pathogens. This book discusses and goes into detail on a number of topics: the molecular basis of nutrient exchange between arbuscular mycorrhizal (AM) fungi and host plants; the role of AM fungi in disease protection, alleviation of soil stresses and increasing grain production; interactions of AM fungi and beneficial saprophytic mycoflora in terms of plant growth promotion; the role of AM fungi in the restoration of native ecosystems; indirect contributions of AM fungi and soil aggregation to plant growth and mycorrhizosphere effect of multitrophic interaction; the mechanisms by which mycorrhizas change a disturbed ecosystem into productive land; the importance of reinstallation of mycorrhizal systems in the rhizosphere is emphasized and their impact on landscape regeneration, and in bioremediation of contaminated soils; Ectomycorrhizae (ECM) and their importance in forest ecosystems and associations of ECM in tropical rain forests function to maintain tropical monodominance; in vitro mycorrhization of micro-propagated plants, and visualizing and quantifying endorhizal fungi; the use of mycorrhizae, mainly AM and ECM, for sustainable agriculture and forestry.

This is the first comprehensive and up-to-date reference on the science, mechanism, methodology, and application of allelopathy. The objective of this practical reference is to report on the latest advances by inviting leading scientists to contribute in specific fields. The volume is organized under three major subsections: History of allelopathy, Allelochemicals, allelopathic mechanisms, and bioassays, and Application of allelopathy in agriculture and forestry.

Megaplasmids are extrachromosomal genetic elements in the size range of 100 kb and larger. They are found in physiologically and phylogenetically diverse groups of bacteria and archaea. By definition, megaplasmids are not essential for the viability of their hosts under all growth conditions, but paradoxically many megaplasmids carry the genetic information for the defining and characteristic traits of the organism in which they reside.

Microbial Megaplasmids reviews our knowledge of the extensively studied representatives, such as the catabolic plasmids of the pseudomonads, the rhizobial Sym plasmids, the Ti plasmids of the genus Agrobacterium and the giant enterobacterial virulence plasmids. It also presents snapshots of more recently discovered megaplasmids. The contribution of megaplasmids to the biology of their hosts is described, highlighting the interactions between megaplasmid and chromosomal genes.

This book will awaken the interest of breeders, phytopathologists, environmentalists, extension services, plant virologists, entomologists and molecular biologists. It deals both with the epidemiological aspects of the disease and with integrated pest management in the field. It discusses the efforts aimed at breeding tomato plants resistant to the virus (using classical breeding, marker-assisted breeding and genetic engineering). It summarizes the techniques used for diagnosis, eradication and certification and emphasizes the problems inherent to the control of the virus insect vector, the use of pesticides and the resistance acquired by the insects, the appearance of new whitefly biotypes with previously unknown characteristics, and the complex relations between virus, vector and plant host.

Bemisia tabaci (Gennedius) has distinguished itself from the more than 1,000 whitefly species in the world by its adaptability, persistence and potential to damage a wide range of agricultural and horticultural crops in all six of the world s inhabited continents. B. tabaci inflicts plant damage through direct feeding, inducement of plant disorders, vectoring of plant viruses and excretion of honeydew. This book collates multiple aspects of the pest ranging from basic to applied science and molecular to landscape levels of investigation. Experts in multiple disciplines provide broad, but detailed summaries and discussion of taxonomy, genetics, anatomy, morphology, physiology, behavior, ecology, symbiotic relationships, virus vector associations and various tactics for integrated management of this pest insect. The book is focused primarily on progress during the last 10-15 years and is directed at workers in the field as well as the informed professional who may not necessarily specialize in whitefly research. The book is unique in providing broad coverage in relatively few chapters by recognized experts that highlight the state-of-the-art in our understanding of this fascinating but troublesome cosmopolitan pest."

This book compiles the latest research in the area of Trichoderma Rhizosphere Biology. It covers topics such as microbial interaction, crosstalk between plants and microbes, interactions with abiotic and biotic factors, and advances in biocontrol agents, biofertilizers and biostimulants. The respective chapters describe innovative ways of adapting fungal communities to improve their survival in highly dynamic environments and agroecosystems. In closing, the book discusses the use of Trichoderma as a bio-growth enhancer and biostimulant for organic agriculture.

The Garden and Greenhouse Flowers manual is a reference manual on diseases which attack garden and greenhouse flowers. The manual identifies various types of diseases which are known to invade these plants located throughout North, Central, and South America.The recordings include diseases caused by fungi, bacteria, viruses, viroids, phytoplasmas, and nematodes. Causal disease agents are described and illustrated in some cases and diseases and disease control measures are also discussed. A manual such as this is never finished since new reports of diseases are continuously reported.

Through Green Revolution in late 1960s, India achieved self-sufficiency in food production, but still the country has not achieved self-sufficiency in production of horticultural crops. Most of the growth in food production during the green revolution period is attributed to the use of higher levels of fertilizers and pesticides which are continuing to destroy stable traditional ecosystems. The challenge before the crop protection scientist is to increase yields from the existing land without harming the environment and resource base. This can be achieved by adopting eco-friendly Biointensive Integrated Pest Management (BIPM) strategy.

BIPM incorporates ecological and economic factors into agricultural system design and decision making, and addresses public concerns about environmental quality and food safety. The benefits of implementing BIPM can include reduced chemical input costs, reduced on-farm and off-farm environmental impacts, and more effective and sustainable pest management. An ecology-based IPM has the potential of decreasing inputs of fuel, machinery, and synthetic chemicals-all of which are energy intensive and increasingly costly in terms of financial and environmental impact. Such reductions will benefit the grower and society.

The present book deals with the most recent biointensive integrated approaches for pest management utilizing components such as bioagents predators, parasitoids and pathogens (bacteria, fungi, viruses)], botanicals (biofumigation, oil cakes, FYM, compost, crop residues, green manuring and other organic amendments), arbuscular mycorrhizal fungi, physical methods (hot water treatment of planting material, soil solarization), cultural methods (crop rotation, summer ploughing, fallowing, intercropping, pruning, mulching, spacing, planting date, trap cropping, "etc."), biorational chemicals (pheromones) and resistant cultivars. This book can serve as a useful reference to policy makers, research and extension workers, practicing farmers and students. The material can also be used for teaching post-graduate courses."

Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.

This book provides an overview of the current state of knowledge of the genetics and genomics of the agriculturally important Cucurbitaceae plant family, which includes crops such as watermelon, melon, cucumber, summer and winter squashes, pumpkins, and gourds. Recent years have resulted in tremendous increases in our knowledge of these species due to large scale genomic and transcriptomic studies and production of draft genomes for the four major species, Citrullus lanatus, Cucumis melo, Cucumis sativus, and Cucurbita spp. This text examines genetic resources and structural and functional genomics for each species group and across species groups. In addition, it explores genomic-informed understanding and commonalities in cucurbit biology with respect to vegetative growth, floral development and sex expression, fruit growth and development, and important fruit quality traits.

The need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays have been successfully applied for the detection of viruses in crop and weed host plant species as well as in the vectors. Nucleic acid-based techniques have been demonstrated to be the most reliable and sensitive tests for detection, identification and differentiation of viruses and viroids present in plants and planting materials.. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume.

Jointly published with INRA, Paris.
Bacterial wilt, caused by "Ralstonia solanacearum," is a very destructive plant disease that attacks over 450 different species, including many of the most important economic crop plants. Often endemic, the bacterium transmits through the soil, penetrates the plant root system and eventually causes irreversible wilting and death. This book summarizes the current information on bacterial wilt for both the basic research community and for concerned professionals who are faced with the disease in the field, offering the latest approaches to diagnosis and control of the disease. Emphasis is placed on integrated and biologically sustainable control methods. Also presented is the most recent genetic/biochemical research exploring the interaction between the bacterium and its plant host at the molecular level.

The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.

The major objective of this book is to highlight the significance of phytonematodes in horticulture. Detailed and latest information on major aspects of phytonematodes associated exclusively with horticultural crops, which is the need of the day, is lacking. Hence, the book has been written mainly with the objective of providing its readers, comprehensive information on the advanced aspects related to phytonematodes associated with horticultural crops. It also provides basic information on plant parasitic nematodes since it is required for a better understanding of advanced topics. Several popular topics, information on which is already available in plenty, have been avoided. Thus, book explicates both the essential fundamental and advanced aspects pertaining to nematodes associated with horticultural crops. The book is conveniently divided into 13 chapters, which cover latest information on the major fundamental and advanced aspects related to phytonematodes including the role of phytonematodes in horticultural industry, phylogenetic and evolutionary concepts in nematodes, major phytonematodes associated with horticultural crops and their diagnostic keys, symptoms caused by phytonematodes and disease diagnosis, nematode population threshold levels, crop loss assessment, nematode diseases of horticultural crops and their management, nematode disease complexes, genetics of nematode parasitism, important nematological techniques and nematodes of quarantine importance. An exclusive chapter on novel methods of nematode management has been included mainly to provide the information on the latest molecules and novel modes of managing nematodes attacking horticultural crops. Routine nematode management aspects, information on which is already available, have not been discussed; instead, this topic reflects the changing scenario of future nematode management. Hence, this book can serve as a friendly guide to meet the requirements of the students, teachers and researchers interested in these 'hidden enemies' of the grower, apart from the research and extension personnel working under Public organizations, officials of State departments of Horticulture, Forestry, field workers and all those concerned and working with plant parasitic nematodes. Appropriate diagrams, convincing tables and suitable graphs/illustrations have been furnished at right places. A complete bibliography has also been included.

A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

This book is based on papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. Since the first symposium (published as Acta Horticulturae 225, 1988), there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centered on attempts to separate, spatially, the problems of vertical disease transmission, via infected explants to progeny microplants, from laboratory contamination. Disease control is best achieved by establishing pathogen-free cultures, while laboratory contamination management is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most important causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality micro-propagules. In this book, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo. The contents are divided into sections. In each section there are papers that update contributions offered at the first symposium; for example, major advances have been made in microbial taxonomy and diagnosticsbased on advances in DNA-based techniques. Consolidation has occurred in therapy and laboratory contamination management. Novel, and arguably speculative, in vitro contamination control based on autotrophic culture is described; and disease control at establishment using bacterial and mycorrhizal inoculants is discussed.

In response to low iron availability in the environment most microorganisms synthesize iron chelators, called siderophores. Bacteria and fungi produce a broad range of structurally diverse siderophores, which all show a very high affinity for ferric ions.

"Microbial Siderophores" presents an up-to-date overview of the chemistry, biology and biotechnology of these iron chelators. Following an introduction to the structure, functions and regulation of fungal siderophores, several chapters focus on siderophores of pseudomonads. Here, the technique of siderotyping, which has proved to be a rapid, accurate and inexpensive tool for pseudomonad characterization and identification, is described. Further, the biological significance of siderophores of symbiotic fungi and the possible role of siderophores in pathogenesis are discussed. In addition to methodological approaches, chapters on the biotechnological production of siderophores and their application in promoting human and plant health are included.

Plants use resources, i.e. carbon, nutrients, water and energy, either for growth or to defend themselves from biotic and abiotic stresses. This volume provides a timely understanding of resource allocation and its regulation in plants, linking the molecular with biochemical and physiological-level processes. Ecological scenarios covered include competitors, pathogens, herbivores, mycorrhizae, soil microorganisms, carbon dioxide/ozone regimes, nitrogen and light availabilities. The validity of the Growth-Differentiation Balance Hypothesis is examined and novel theoretical concepts and approaches to modelling plant resource allocation are discussed. The results presented can be applied in plant breeding and engineering, as well as in resource-efficient stand management in agriculture and forestry.

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For investigators engaged in the study of toxins generally, and host-specific toxins in particular, it is a rare treat to attend a meeting in which toxins involved in plant pathogenesis are emphasized. A gathering of this type provides opportunity to consider the discovery of new toxins, their chemical structures, genes encoding enzymes that control their biosyntheses, their sites of action and physiological effects on plants, and their roles (if any) in pathological processes. Having acknowledged the inspiration fostered by a 'toxin meeting', however, it is important to point out that the program of this symposium was generously sprinkled with 'nontoxin' talks. These contributions generated cross-disciplinary discussion and promoted new ways of thinking about relationships among factors required for plant disease development. The point can be illustrated by considering just one example. We have in the past often regarded diseases mediated by host-specific toxins and diseases involving 'gene-for-gene' relationships as representing two different classes of fungal/plant interaction. This is largely because the key molecular recognition event in so-called 'toxin' diseases leads to compatibility, whereas the corresponding event in 'gene-for-gene' diseases leads to incompatibility. Yet the race specific elicitors produced by the 'gene-for-gene' fungi Cladosporium fulvum (De Wit, Adv. Bot. Res. 21:147- 185, 1995) and Rhynchosporium secalis (Rohe et a1. , EMBO J.

Westcott's Plant Disease is a reference book on diseases which attack plants. Diseases of plants are found on most all plants including trees, shrubs, grasses, forage, fruits, vegetables, garden and greenhouse plants as well as native wild flowers and even weeds. Plant Disease Handbook identifies various types of diseases which are known to invade these plants located throughout North and South America. The recordings include diseases caused by fungi, bacteria, viruses, viroids and nematodes. Causal disease agents are described and illustrated in many cases and diseases and disease control measures are also discussed. A book such as this is never finished since new reports of diseases are continuously reported. This includes new diseases and previously known diseases which occur on both presently recorded plants and on new plants found to be susceptible to diseases. Westcott's Plant Disease provides a reference and guide for identification and control of these plant disease problems.

''Informative, well-constructed, and readable...The contributors are leaders in their fields and what they have to say is worthwhile.'' --- SGM Quarterly, August 1998

Pine forests face a global threat of pine wilt disease, which is being spread by vector beetles carrying pathogenic nematodes from dead trees to healthy ones. Among the host pines there are varying degrees of susceptibility, and nematode strains also contain a variety of virulences, both of which factors help to determine whether infected host trees will die or survive. As well, biotic and abiotic environmental factors influence the fate of infected trees. This book describes the history of the disease, pathogenic nematodes, vector beetles, the etiology and ecology of the disease, microorganisms involved, and control methods that utilize host resistance and biological control agents. Concrete, comprehensive, and the most up-to-date knowledge about this worldwide forest epidemic is presented for readers, enabling them to understand the nature and epidemic threat of pine wilt disease.

Agricultural research is one of the most widespread forms of organized research in the world, in both developed and developing countries. Management of agricultural research involves many decisions that have scientific, social and political consequences. Every country has established agricultural research priorities based on many complex factors that must be considered when decisions are made on the choice of research problems to be investigated. Resources must be divided among projects that often compete for the limited funding available that supports the total research enterprise. This book will provide those individuals who manage research with methods and procedures that can be used in their management organization.