This book is a biography of a scientist who pioneered the development of plant pathology in Australia in the 19th and early 20th century, and was internationally acclaimed. After 20 years as a plant pathologist, he was asked to find the cause and cure of a serious physiological disorder of apples. While the cause eluded him, and everyone else for another 60 years, he again won international gratitude for the improvements he brought to the apple industry. However because he did not find the cause, he was deemed to have failed by his political masters who were malignantly influenced by a jealous rival. The discovery in 2012-2013 of government files covering the period of the bitter pit investigation, from 1911 to 1916; reveal the extent of the unjust criticism of McAlpine while history has vindicated the management recommendations made to reduce bitter-pit losses. The focus on bitter-pit management late in McAlpine's Career also meant that those who value his memory have been less aware of the remarkable achievements of McAlpine in the time before he left Great Britain - the brilliance of his teaching and drawing skills -featured in the early teaching texts for botany and zoology (the latter with his brother) which are now accessible on-line. The objective of this book is to demonstrate that (i) the view that McAlpine had failed in his quest was wrong and seriously unjust (ii) McAlpine achievements extend beyond plant pathology and include significant contributions to the 19th century teaching of botany and zoology, contributions which reinforce the adage - a picture is worth a 1,000 words.

This book is a first attempt to link well-known plant resistance phenomena with emerging concepts in molecular biology. Resistance phenomena such as the local lesion response, induced resistance, "green islands" and resistance in various crop plants are linked with new information on gene-silencing mechanisms, gene silencing suppressors, movement proteins and plasmodesmatal gating, downstream signalling components, and more.

This book presents a collection of papers that give a comprehensive description of the major areas of research on the interactions between plants and their pathogens. State-of-the-art knowledge about research on microbial avirulence genes and plant resistance genes, about pathogenicity factors and signals for establishment of infection, about elicitors and intermediates in the signalling pathway, and about the genes involved in plant responses to pathogens and environmental stress is reviewed. The different topics covered show that modern tools of plant cellular and molecular biology are now available to investigate very significant models of plant-pathogen interactions and to identify the molecular determinants of the two partners that trigger defence responses, the signals involved and the mechanism of their transduction, the spatial and temporal regulation of defence gene expression in relation to the spread or localization of the pathogen. The first trials to exploit these results for enhancing plant resistance and crop field by biotechnology are presented. The book is a main reference source for research scientists and advanced students.

Plant disease epidemiology is a dynamic science that forms an essential part of the study of plant pathology. This book brings together a team of 35 international experts. Each chapter deals with an essential component of the subject and allows the reader to fully understand how each exerts its influence on the progress of pathogen populations in plant populations over a defined time scale. Since the first edition of the text was published in 1998, many new developments have occurred in the subjects covered, particularly molecular diagnostics, modelling, fungicide resistance and information technology. The second edition of the book is a comprehensive text on all aspects of plant disease epidemiology that should serve as an invaluable reference work for those involved in this fascinating science of crop plants.

The fifth and last Volume of this IPMD series reviews, in a multi-disciplinary approach, recent achievements in crop protection and integrated management of arthropod pests. The volume is organized in a first Section covering IPM in citrus productions, a Second one dealing with advacements in the integratioon of management technologies and a last Section covering mites and their biological control agents. As for the previous volumes, we attempted to provide an informative coverage for a broad range of agricultural systems and situations. The chapters are mainly organized and centered on crops, with a particular emphasis on citrus. This is one of the main crops in which biological control and IPM approaches were tested successfully, and the experience gained herein may indeed result helpful for IMP efforts deployed worldwide on other crops and/or cropping systems. Chapters in Section 2 review the integration of insect and disease management options in pecan crops, the application of remote sensing technologies, the status of knowledge about plant defense compounds and their potentials. For IPM of invasive species, an update is provided on the experience gained on the Red Palm Weevil (RPW) in Egypt. Long-term technological solutions for IPM in forests and park areas through aerial treatments with Bt spores concludes this Section. Finally, in Section 3, updated reviews about biological control agents of mites are provided.

Raspberry is a globally-significant soft fruit crop, with increasing interest to consumers due to its versatility and health-related constituents. In this background context, it is therefore timely to consider the present and future status of the raspberry crop, particularly with the advances in the use of molecular tools and plant phenotyping to improve our understanding of improving crop quality and fruit yields. Since the 1980s a wealth of fundamental genomics and metabolomics resources have been developed for soft fruits including linkage maps, physical maps, QTLs and expression tools. However, a number of serious and emerging challenges exist for the raspberry industry, including the plants' ability to resist major pest and disease burdens and the impact of climate change on crop production, specifically water use and water availability for soft fruit crops. This book aims to address some of these challenges by updating the information known about this important crop, its health value, the major pest and diseases which affect raspberry and approaches for their control, and the speed and precision offered by selective breeding programs by the deployment of molecular tools and linkage maps for germplasm assessment. Understanding the genetic control of commercially and nutritionally important traits and the linkage of these characteristics to molecular markers on chromosomes is the future basis of plant breeding. We will also introduce the opportunity to fast track breeding by improving the speed of phenotypic selection by utilizing imaging sensor technologies, thereby reducing the cost of years of field assessment through developing this knowledge into markers linked to key fruit traits. The chapters of this book will span the knowledge gained from the collaborations between growers, plant breeders, plant physiologists, soil scientists, geneticists, agronomists and physicists which is essential to achieve progress in improving productivity and a sustainable industry.

This book presents advanced ecological techniques for crop cultivation and the chapters are arranged into four sections, namely general aspects, weeds, fungi, worms and microbes. Biocontrol is an ecological method of controlling pests such as insects, mites, weeds and plant diseases using other organisms. This practice has been used for centuries. Biocontrol relies on predation, parasitism, herbivory, or other natural mechanisms. Natural enemies of insect pests, also known as biological control agents, include predators, parasitoids, pathogens, and competitors.

This book provides a comprehensive discussion on plant responses in hyperarid regions of Egypt, China, Mexico, and Pakistan. It describes their location, physiographic features, accidental vegetation along two transects, endangered vegetation species, human impact, and variety of plant types (e.g. climbing, succulent, and parasitic). Studies on biotic and abiotic interactions, plant biodiversity, and soil-plant relationships are also covered. Covering a wide range of plant conditions and adaptations, this book analyzes what happens when plants must endure very high temperatures and aridity. Plants have adapted by evolving their physical structure to store and conserve water. Examples are the absence of leaves which reduces transpiration and the growth of extremely long roots, allowing them to acquire moisture at, or near the water table. Plants in hyperarid habitats have also made behavioral adaptations in order to survive by synchronizing with the seasons of greatest moisture and/or coolest temperatures. For example, desert perennials remain dormant during dry periods of the year, then spring to life when water becomes available. The book includes many color illustrations, and has extensive and up-to-date references for further reading.

Mycorrhizal fungi are microbial engines which improve plant vigor and soil quality. They play a crucial role in plant nutrient uptake, water relations, ecosystem establishment, plant diversity, and the productivity of plants. Scientific research involves multidisciplinary approaches to understand the adaptation of mycorrhizae to the rhizosphere, mechanism of root colonization, effect on plant physiology and growth, biofertilization, plant resistance and biocontrol of plant pathogens. This book discusses and goes into detail on a number of topics: the molecular basis of nutrient exchange between arbuscular mycorrhizal (AM) fungi and host plants; the role of AM fungi in disease protection, alleviation of soil stresses and increasing grain production; interactions of AM fungi and beneficial saprophytic mycoflora in terms of plant growth promotion; the role of AM fungi in the restoration of native ecosystems; indirect contributions of AM fungi and soil aggregation to plant growth and mycorrhizosphere effect of multitrophic interaction; the mechanisms by which mycorrhizas change a disturbed ecosystem into productive land; the importance of reinstallation of mycorrhizal systems in the rhizosphere is emphasized and their impact on landscape regeneration, and in bioremediation of contaminated soils; Ectomycorrhizae (ECM) and their importance in forest ecosystems and associations of ECM in tropical rain forests function to maintain tropical monodominance; in vitro mycorrhization of micro-propagated plants, and visualizing and quantifying endorhizal fungi; the use of mycorrhizae, mainly AM and ECM, for sustainable agriculture and forestry.

Insect and disease issues are often specific to the Mediterranean forest systems rather than shared with the temperate forests. In addition to the specific native insects and diseases, the forests are subject to the invasion of exotic species. The forests are also at risk from high degrees of human activity, including changing patterns of forest fires, land management activities, intensive plantation forestry using introduced timber species from other Mediterranean climate zones, and atmospheric deposition. Combined with elements of global climate change that may disproportionately affect Mediterranean climate systems, this creates a number of significant management issues that are unique to the Mediterranean forests. It is our goal that the information contained in this volume will contribute to understanding the unique aspects of Mediterranean forest systems and to protecting these critical resources.

This is the first comprehensive and up-to-date reference on the science, mechanism, methodology, and application of allelopathy. The objective of this practical reference is to report on the latest advances by inviting leading scientists to contribute in specific fields. The volume is organized under three major subsections: History of allelopathy, Allelochemicals, allelopathic mechanisms, and bioassays, and Application of allelopathy in agriculture and forestry.

Megaplasmids are extrachromosomal genetic elements in the size range of 100 kb and larger. They are found in physiologically and phylogenetically diverse groups of bacteria and archaea. By definition, megaplasmids are not essential for the viability of their hosts under all growth conditions, but paradoxically many megaplasmids carry the genetic information for the defining and characteristic traits of the organism in which they reside.

Microbial Megaplasmids reviews our knowledge of the extensively studied representatives, such as the catabolic plasmids of the pseudomonads, the rhizobial Sym plasmids, the Ti plasmids of the genus Agrobacterium and the giant enterobacterial virulence plasmids. It also presents snapshots of more recently discovered megaplasmids. The contribution of megaplasmids to the biology of their hosts is described, highlighting the interactions between megaplasmid and chromosomal genes.

This book will awaken the interest of breeders, phytopathologists, environmentalists, extension services, plant virologists, entomologists and molecular biologists. It deals both with the epidemiological aspects of the disease and with integrated pest management in the field. It discusses the efforts aimed at breeding tomato plants resistant to the virus (using classical breeding, marker-assisted breeding and genetic engineering). It summarizes the techniques used for diagnosis, eradication and certification and emphasizes the problems inherent to the control of the virus insect vector, the use of pesticides and the resistance acquired by the insects, the appearance of new whitefly biotypes with previously unknown characteristics, and the complex relations between virus, vector and plant host.

Bemisia tabaci (Gennedius) has distinguished itself from the more than 1,000 whitefly species in the world by its adaptability, persistence and potential to damage a wide range of agricultural and horticultural crops in all six of the world s inhabited continents. B. tabaci inflicts plant damage through direct feeding, inducement of plant disorders, vectoring of plant viruses and excretion of honeydew. This book collates multiple aspects of the pest ranging from basic to applied science and molecular to landscape levels of investigation. Experts in multiple disciplines provide broad, but detailed summaries and discussion of taxonomy, genetics, anatomy, morphology, physiology, behavior, ecology, symbiotic relationships, virus vector associations and various tactics for integrated management of this pest insect. The book is focused primarily on progress during the last 10-15 years and is directed at workers in the field as well as the informed professional who may not necessarily specialize in whitefly research. The book is unique in providing broad coverage in relatively few chapters by recognized experts that highlight the state-of-the-art in our understanding of this fascinating but troublesome cosmopolitan pest."

The Garden and Greenhouse Flowers manual is a reference manual on diseases which attack garden and greenhouse flowers. The manual identifies various types of diseases which are known to invade these plants located throughout North, Central, and South America.The recordings include diseases caused by fungi, bacteria, viruses, viroids, phytoplasmas, and nematodes. Causal disease agents are described and illustrated in some cases and diseases and disease control measures are also discussed. A manual such as this is never finished since new reports of diseases are continuously reported.

Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.

Through Green Revolution in late 1960s, India achieved self-sufficiency in food production, but still the country has not achieved self-sufficiency in production of horticultural crops. Most of the growth in food production during the green revolution period is attributed to the use of higher levels of fertilizers and pesticides which are continuing to destroy stable traditional ecosystems. The challenge before the crop protection scientist is to increase yields from the existing land without harming the environment and resource base. This can be achieved by adopting eco-friendly Biointensive Integrated Pest Management (BIPM) strategy.

BIPM incorporates ecological and economic factors into agricultural system design and decision making, and addresses public concerns about environmental quality and food safety. The benefits of implementing BIPM can include reduced chemical input costs, reduced on-farm and off-farm environmental impacts, and more effective and sustainable pest management. An ecology-based IPM has the potential of decreasing inputs of fuel, machinery, and synthetic chemicals-all of which are energy intensive and increasingly costly in terms of financial and environmental impact. Such reductions will benefit the grower and society.

The present book deals with the most recent biointensive integrated approaches for pest management utilizing components such as bioagents predators, parasitoids and pathogens (bacteria, fungi, viruses)], botanicals (biofumigation, oil cakes, FYM, compost, crop residues, green manuring and other organic amendments), arbuscular mycorrhizal fungi, physical methods (hot water treatment of planting material, soil solarization), cultural methods (crop rotation, summer ploughing, fallowing, intercropping, pruning, mulching, spacing, planting date, trap cropping, "etc."), biorational chemicals (pheromones) and resistant cultivars. This book can serve as a useful reference to policy makers, research and extension workers, practicing farmers and students. The material can also be used for teaching post-graduate courses."

This book compiles the latest research in the area of Trichoderma Rhizosphere Biology. It covers topics such as microbial interaction, crosstalk between plants and microbes, interactions with abiotic and biotic factors, and advances in biocontrol agents, biofertilizers and biostimulants. The respective chapters describe innovative ways of adapting fungal communities to improve their survival in highly dynamic environments and agroecosystems. In closing, the book discusses the use of Trichoderma as a bio-growth enhancer and biostimulant for organic agriculture.

The need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays have been successfully applied for the detection of viruses in crop and weed host plant species as well as in the vectors. Nucleic acid-based techniques have been demonstrated to be the most reliable and sensitive tests for detection, identification and differentiation of viruses and viroids present in plants and planting materials.. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume.

Jointly published with INRA, Paris.
Bacterial wilt, caused by "Ralstonia solanacearum," is a very destructive plant disease that attacks over 450 different species, including many of the most important economic crop plants. Often endemic, the bacterium transmits through the soil, penetrates the plant root system and eventually causes irreversible wilting and death. This book summarizes the current information on bacterial wilt for both the basic research community and for concerned professionals who are faced with the disease in the field, offering the latest approaches to diagnosis and control of the disease. Emphasis is placed on integrated and biologically sustainable control methods. Also presented is the most recent genetic/biochemical research exploring the interaction between the bacterium and its plant host at the molecular level.

This book provides an overview of the current state of knowledge of the genetics and genomics of the agriculturally important Cucurbitaceae plant family, which includes crops such as watermelon, melon, cucumber, summer and winter squashes, pumpkins, and gourds. Recent years have resulted in tremendous increases in our knowledge of these species due to large scale genomic and transcriptomic studies and production of draft genomes for the four major species, Citrullus lanatus, Cucumis melo, Cucumis sativus, and Cucurbita spp. This text examines genetic resources and structural and functional genomics for each species group and across species groups. In addition, it explores genomic-informed understanding and commonalities in cucurbit biology with respect to vegetative growth, floral development and sex expression, fruit growth and development, and important fruit quality traits.

The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.

This book is based on papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. Since the first symposium (published as Acta Horticulturae 225, 1988), there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centered on attempts to separate, spatially, the problems of vertical disease transmission, via infected explants to progeny microplants, from laboratory contamination. Disease control is best achieved by establishing pathogen-free cultures, while laboratory contamination management is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most important causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality micro-propagules. In this book, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo. The contents are divided into sections. In each section there are papers that update contributions offered at the first symposium; for example, major advances have been made in microbial taxonomy and diagnosticsbased on advances in DNA-based techniques. Consolidation has occurred in therapy and laboratory contamination management. Novel, and arguably speculative, in vitro contamination control based on autotrophic culture is described; and disease control at establishment using bacterial and mycorrhizal inoculants is discussed.

A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.