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Books > Medicine > Clinical & internal medicine > Diseases & disorders > Immunology
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The P2X7 Receptor - Methods and Protocols (Hardcover, 1st ed. 2022)
Loot Price: R6,288
Discovery Miles 62 880
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The P2X7 Receptor - Methods and Protocols (Hardcover, 1st ed. 2022)
Series: Methods in Molecular Biology, 2510
Expected to ship within 12 - 17 working days
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This detailed volume covers diverse aspects of P2X7 receptor
analysis, ranging from its molecular structure to related
pharmacological and immunological tools, via its analysis in
heterologous expression systems as well as assays using primary
cells and whole animal models. After three introductory chapters
that focus on its structure, ligands, and physiological functions,
the book details the generation of antibody and nanobody tools for
P2X7 receptors, provides protocols for the analysis of expressed
P2X7 receptors with a focus on their electrophysiological analysis,
as well as protocols for the investigation of P2X7 down-stream
signaling in immune cells by flow cytometry. Mouse models and
procedures suited to investigate P2X7-mediated effects in other
primary cells and in vivo are also explained. Written for the
highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, The P2X7
Receptor: Methods and Protocols is a valuable reference not only
for the growing community fascinated by this unusual ion channel
but also for a broad readership interested in ion channels or
purinergic receptors.
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