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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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SELDI-TOF Mass Spectrometry - Methods and Protocols (Hardcover, 2012)
Loot Price: R3,067
Discovery Miles 30 670
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SELDI-TOF Mass Spectrometry - Methods and Protocols (Hardcover, 2012)
Series: Methods in Molecular Biology, 818
Expected to ship within 10 - 15 working days
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SELDI is distinct from other TOF-MS technologies in that it couples
features of chromatography and mass spectrometry, facilitating
analyte enrichment and sample cleanup on an array surface. In the
growing field of proteomics, SELDI technology has been widely used
for biomarker discovery and characterization in diverse
applications including diagnostics, drug development, and basic
research. SELDI-based biomarker studies can typically be divided
into four phases: discovery, validation, purification and
identification, and assay development. SELDI-TOF Mass Spectrometry:
Methods and Protocols provides an overview of the current
applications of SELDI-TOF MS (surface enhanced laser
desorption/ionization time-of-flight mass spectrometry), with an
emphasis on study and experimental design, data analysis and
interpretation, and assay development. Written in the highly
successful Methods in Molecular Biology (TM) series format,
chapters contain introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and notes on troubleshooting and
avoiding known pitfalls. Authoritative and accessible, SELDI-TOF
Mass Spectrometry: Methods and Protocols will provide information
on optimizing study design, experimental protocols, and data
analysis and interpretation to yield robust biomarkers and
biomarker assays, using examples from different disease areas.
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