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Spectral Techniques In Proteomics (Paperback)
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Spectral Techniques In Proteomics (Paperback)
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Facilitating the innovation, development, and application of new
spectroscopic methods in proteomics, Spectral Techniques in
Proteomics provides a broad overview of the spectroscopic toolbox
that can be used, either with proteome or sub-proteome mixtures or
with individual/purified proteins studied in parallel. It gives a
modest overview of existing and proven techniques as well as a
detailed examination of less established spectroscopic methods with
studied speculation on future applications. Intended for a broad
audience of protein biochemists and biophysicists, the book adopts
a wider definition of proteomics to include the systems-based study
of proteomes and sub-proteomes involving proteins related through
regulatory cascades, metabolic pathways, post-translational
modifications, or associated biologic effect, as well as the
parallel study of subsets such as proteins with associated protein
folds (structural proteomics) or binding sites (chemical
proteomics). Beginning by defining the scope of the field as is
relevant to spectroscopists, the book then briefly reviews current
commonly used spectroscopic methods. It covers separation
techniques that typically precede ESI studies as well as MALDI
MS/MS based protein identification. SELDI is also presented as a
tool that combines separation techniques with MS analysis on the
same chip. The book presents studies of protein-protein and
protein-ligand interactions using NIR fluorescence, NMR, MS, and
SPR. Recent developments in ICAT labeling strategies are addressed
along with a discussion of metabolomics. A description of advances
in structural proteomics using NMR, x-ray crystallography, and EPR
precedes a final summary of current technology and future prospects
of the science. Analyzing the current state of the science and the
future evolution of the field, Spectral Techniques in Proteomics
applies a systems-based approach to studying the function and
mechanism of proteins.
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