Analytical techniques based on separation processes, such as
chromatography and electrophoresis, are finding a growing range of
applications in chemical, pharmaceutical and clinical laboratories.
The Wiley Separation Science Series provides the analyst in these
laboratories with well focused books covering individual
techniques, so that they can be applied more efficiently and
effectively to contemporary analytical problems. In biotechnology,
biochemistry and molecular biology, the characterization and
analysis of biomolecules such as proteins, oligosaccharides and
nucleic acids are of great importance. High performance liquid
chromatography (HPLC) has become one of the key analytical tools in
these fields, providing rapid purification and quantitative
analysis of biomolecules.
High Performance Liquid Chromatography: Principles and Methods
in Biotechnology covers the most important theoretical and
practical aspects of the application of HPLC to the biosciences,
including sample preparation, column selection, detection of
biomolecules, preparative HPLC, and the use of HPLC for specific
biomolecules. Finally, the technique is compared to alternative
bioseparation methods. The ten chapters are written by researchers
active in the biotechnology field. High Performance Liquid
Chromatography: Principles and Methods in Biotechnology will be of
great practical use to molecular biologists, biochemists and
biotechnologists who use HPLC to purify and quantify biomolecules.
It will also be of interest to those carrying out protein and DNA
characterization and analysis.
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