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Books > Science & Mathematics > Physics > Applied physics & special topics > Biophysics
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Protein Folding and Misfolding - Shining Light by Infrared Spectroscopy (Hardcover, 2012)
Loot Price: R2,672
Discovery Miles 26 720
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Protein Folding and Misfolding - Shining Light by Infrared Spectroscopy (Hardcover, 2012)
Series: Biological and Medical Physics, Biomedical Engineering
Expected to ship within 18 - 22 working days
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Infrared spectroscopy is a new and innovative technology to study
protein folding/misfolding events in the broad arsenal of
techniques conventionally used in this field. The progress in
understanding protein folding and misfolding is primarily due to
the development of biophysical methods which permit to probe
conformational changes with high kinetic and structural resolution.
The most commonly used approaches rely on rapid mixing methods to
initiate the folding event via a sudden change in solvent
conditions. Traditionally, techniques such as fluorescence,
circular dichroism or visible absorption are applied to probe the
process. In contrast to these techniques, infrared spectroscopy
came into play only very recently, and the progress made in this
field up to date which now permits to probe folding events over the
time scale from picoseconds to minutes has not yet been discussed
in a book. The aim of this book is to provide an overview of the
developments as seen by some of the main contributors to the field.
The chapters are not intended to give exhaustive reviews of the
literature but, instead to illustrate examples demonstrating the
sort of information, which infrared techniques can provide and how
this information can be extracted from the experimental data. By
discussing the strengths and limitations of the infrared approaches
for the investigation of folding and misfolding mechanisms this
book helps the reader to evaluate whether a particular system is
appropriate for studies by infrared spectroscopy and which specific
advantages the techniques offer to solve specific problems.
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