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Mass Spectrometry and Genomic Analysis (Hardcover, 2001 ed.)
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Mass Spectrometry and Genomic Analysis (Hardcover, 2001 ed.)
Series: Focus on Structural Biology, 2
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76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline
76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3.
Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass
Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass
Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4.
Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and
Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5.
Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81
5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using
SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86
6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations
in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8.
Future Perspectives 90 9. Acknowledgements 91 10. References 91
CHAPTER 7 WV. Bienvenut, M. Muller, PM. Palagi, E. Gasteiger, M.
Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes,
JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass
Spectrometry: Some Aspects and Recent Developments 1. Introduction
to Proteomics 93 2. Protein Biochemical and Chemical Processing
Followed by Mass Spectrometric Analysis 94 2. 1. 2-DE Gel Protein
Separation 95 Protein Identification Using Peptide Mass
Fingerprinting and Robots 96 2. 2. 2. 2. 1. MALDI-MS Analysis 98 2.
2. 2. MS/MS Analysis 102 Improvement of the Identification by
Chemical Modification of Peptides 106 2. 2. 3."
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