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Books > Science & Mathematics > Physics > Applied physics & special topics > Biophysics
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Fluorescence Microscopy and Fluorescent Probes (Paperback, Softcover reprint of the original 1st ed. 1996)
Loot Price: R3,682
Discovery Miles 36 820
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Fluorescence Microscopy and Fluorescent Probes (Paperback, Softcover reprint of the original 1st ed. 1996)
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Fluorescence microscopy images can be easily integrated into
current video and computer image processing systems. People like
visual observation; they like to watch a television or computer
screen, and fluorescence techniques are thus becoming more and more
popular. Since true in vivo experiments are simple to perform,
samples can be directly seen and there is always the possibility of
manipulating the samples during the experiments; it is an ideal
technique for biology and medicine. Images are obtained by a
classical (now called wide-field) fluorescence microscope, a
confocal scanning microscope, upright or inverted, with
epifluorescence or transmission. Computerized image processing may
improve definition, and remove glare and scattered light signal. It
also makes it possible to compute ratio images (ratio imaging both
in excitation and in emission) or lifetime imaging. Image analysis
programs may supply a great deal of additional data of various
types, starting with calculations of the number of fluorescent
objects, their shapes, brightness, etc. Fluorescence microscopy
data may be complemented by classical measurement in the cuvette yr
by flow cytometry.
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