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Drugs of Abuse - Neurological Reviews and Protocols (Hardcover, 2003 ed.)
Loot Price: R4,439
Discovery Miles 44 390
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Drugs of Abuse - Neurological Reviews and Protocols (Hardcover, 2003 ed.)
Series: Methods in Molecular Medicine, 79
Expected to ship within 10 - 15 working days
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Drugs of Abuse: Neurological Reviews and Protocols is intended to
provide insightful reviews of key current topics and, particularly,
state-- the-art methods for examining drug actions in their various
neuroanato- cal, neurochemical, neurophysiological,
neuropharmacological, and molecular perspectives. The book should
prove particularly useful to n- comers (graduate students and
technicians) in this field, as well as to those established
scientists (neuroscientists, biochemists, and molecular biologists)
intending to pursue new careers or directions in the study of
drugs. The book's protocols cover a wide variety of coherent
methods for gathering inf- mation on quantitative changes in
proteins and mRNAs at both tissue and cel- lar levels. Inducible
gene expression in striatal neurons has been a hot topic over the
last decade. Alterations in gene expression for a wide range of
proteins in the striatum have been investigated in response to drug
administration. Altered expression of given mRNAs and their product
proteins constitutes essential molecular steps in the development
of neuroplasticity related to long-term addictive properties of
drugs of abuse. With the multiple labeling methods that are also
described in the book, gene expression can be detected in a
chemically identified cell phenotype; the expression of multiple
genes of interest can be detected in a single cell simultaneously.
Hundreds or thousands of gene expr- sion products can today be
detected in one experimental setup using the pow- ful systematic
cDNA macroarray or microarray screening technology. Moreover,
protocols useful in analyzing the functional roles of genes and
proteins (e. g.
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