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Plant Cell and Tissue Culture - A Tool in Biotechnology - Basics and Application (Hardcover, 2009 ed.)
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Plant Cell and Tissue Culture - A Tool in Biotechnology - Basics and Application (Hardcover, 2009 ed.)
Series: Principles and Practice
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The adv antages of those systems are counterbalanced by some
important dis- vantages. For one, in heterotrophic and mixotrophic
systems high concentrations of organic ingredients are required in
the nutrient medium (particularly sugar at 2% or more), associated
with a high risk of microbial contamination. How, and to which
extent this can be avoided will be dealt with in Chapter 3. Other
disadvantages are the difficulties and limitations of extrapolating
results based on tissue or cell c- tures, to interpreting phenomena
occurring in an intact plant during its development. It has always
to be kept in mind that tissue cultures are only model systems,
with all positive and negative characteristics inherent of such
experimental setups. To be realistic, a direct duplication of in
situ conditions in tissue culture systems is still not possible
even today in the 21st century, and probably never will be. The
organization of the genetic system and of basic cell structures is,
however, essentially the same, and therefore tissue cultures of
higher plants should be better suited as model s- tems than, e.g.,
cultures of algae, often employed as model systems in physiological
or biochemical investigations. The domain cell and tissue culture
is rather broad, and necessarily unspecif ic. In terms of practical
aspects, basically five areas can be distinguished (see Figs. 1.1,
1.2 ), which here shall be briefly surveyed before being discussed
later at length.
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