This manual reflects practical approaches to handling bacteria in
the labora- tory. It is designed to recall historical methods of
bacterial genetics that have had recent developments and to present
new techniques that allow full genome analysis. It has been written
for microbiologists who need to group their protocols at the state
of the art of a new millennium and also for scientists in other
fields of life sciences who need to use bacteria for their
research. Teachers, graduate students, and postdocs also will
benefit from having these protocols to help them understand modern
bacterial genetics. I learned so much from these contributions from
my colleagues that I have no doubt about the daily usefulness of
this book. April 2002 Michel Blot XII Abbreviations Acyl-HSL N-acyl
homoserine lactone moi multiplicity of infection Amp or Ap
ampicillin N amino C carboxy NMR nuclear magnetic resonance CIO-HSL
N-decanoyl-L-homoserine lactone 3-0H-C14:1-HSL N-(3-hydroxy-7
-cis-tetra- C12-HSL N-dodecanoyl-L-homoserine lac-
decanoyl)homo-serine lactone tone 3-0H-C4-HSL
N-3-hydroxybutanoyl-L- C14-HSL N-tetradecanoyl-L-homoserine
homoserine lactone lactone ONPG o-nitrophenyl ~-D-galactopyranoside
C4-HSL N-butanoyl-L-homoserine lactone ORF open reading frame
C6-HSL N-hexanoyl-L-homoserine lactone OTG
I-S-octyl-~-D-thioglucoside C8-HSL N-octanoyl-L-homoserine lactone
3-oxo-CIO-HSL N-3-oxodecanoyl-L-homo- Cam or Cm chloramphenicol
serine lactone CBD chitin binding domain 3-oxo-C12-HSL
N-3-oxododecanoyl-L- CHEF contour clamped homogenous electric
homoserine lactone field 3-oxo-C14-HSL N-3-oxotetradecanoyl-L- CI
consistency index homoserine lactone CRIM conditional-replication,
integration, 3-oxo-C4-HSL N-3-oxobutanoyl-L-homoser- and modular
ine lactone dCTP deoxycytidine triphosphate 3-oxo-C6-HSL N-3
-oxohexanoyl-L-homoser- deg.
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