Advanced Photon Counting - Applications, Methods, Instrumentation (Hardcover, 2015 ed.)

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a "fingerprint" for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Foerster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

General

Imprint:	Springer International Publishing AG
Country of origin:	Switzerland
Series:	Springer Series on Fluorescence, 15
Release date:	April 2015
First published:	2015
Editors:	Peter Kapusta • Michael Wahl • Rainer Erdmann
Dimensions:	235 x 155 x 25mm (L x W x T)
Format:	Hardcover
Pages:	370
Edition:	2015 ed.
ISBN-13:	978-3-319-15635-4
Categories:	Books > Science & Mathematics > Physics > Optics (light) Books > Professional & Technical > Electronics & communications engineering > Electronics engineering > Applied optics > General Books > Science & Mathematics > Chemistry > Analytical chemistry > Qualitative analytical chemistry > Chemical spectroscopy, spectrochemistry > General
LSN:	3-319-15635-7
Barcode:	9783319156354

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