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Sedimentation Velocity Analytical Ultracentrifugation - Discrete Species and Size-Distributions of Macromolecules and Particles (Hardcover)
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Sedimentation Velocity Analytical Ultracentrifugation - Discrete Species and Size-Distributions of Macromolecules and Particles (Hardcover)
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A follow-up to the experimental and instrumental aspects described
in Basic Principles of Analytical Ultracentrifugation, the volume
Sedimentation Velocity Analytical Ultracentrifugation: Discrete
Species and Size-Distributions of Macromolecules and Particles
describes the theory and practice of data analysis. Mathematical
models for the sedimentation process and the evolution of detected
signals are developed in a comprehensive framework, jointly with
the description of current and historical strategies for how to
extract from noisy experimental data the physical parameters of
interest, such as size, mass, and shape, composition, and
polydispersity of sedimenting particles. The methods are
extensively illustrated, and supported with practical applications,
as well as cross-references where to find the methods in the public
domain software SEDFIT and SEDPHAT. The systems covered are
discrete or polydisperse mixtures of sedimenting molecules or
particles in dilute solution, such as proteins and other
biomolecules and their stable complexes, man-made polymers, and
nanoparticles, observed in different optical systems. A useful
reference for researchers and graduate students of macromolecular
disciplines, these methods form the essential foundation for the
analysis of dynamic interacting systems, which are covered in the
volume Sedimentation Velocity Analytical Ultracentrifugation:
Interacting Systems. Software referenced in the book is available
for download at: https://sedfitsedphat.nibib.nih.gov/default.aspx
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