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Genetic Library Construction and Screening - Advanced Techniques and Applications (Paperback, 2002 ed.)
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Genetic Library Construction and Screening - Advanced Techniques and Applications (Paperback, 2002 ed.)
Series: Springer Lab Manuals
Expected to ship within 10 - 15 working days
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Designed as an introductory text the authors cover all core
strategies in the application of modern recombinant DNA technology.
The first chapters directly address the applications of polymerase
chain reaction to a variety of problems in DNA cloning that are, or
have been, extremely challenging using more traditional approaches
and technologies. These include cDNA cloning and transcript
mapping, mutagenesis as well as the cloning of very long
transcripts and protocols using limiting amounts of total RNA.
Further chapters describe approaches to subtractive cloning
technologies as well as novel specialized expression cloning and
library screening strategies. The handbook contains detailed
step-by-step protocols and extensive hands-on advice.
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