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Books > Science & Mathematics > Biology, life sciences > Molecular biology
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The Nucleic Acid Protocols Handbook (Hardcover, 2000 ed.)
Loot Price: R5,893
Discovery Miles 58 930
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The Nucleic Acid Protocols Handbook (Hardcover, 2000 ed.)
Expected to ship within 10 - 15 working days
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There can be no doubt that some ofthe most spectacular advances
made in science over the past few decades have been in the
isolation, analysis, and manipulation of nucleicacids. Thishas
ledtoamuchgreaterunderstandingofmechanismsandprocesses across many
fields of bioscience, such as biochemistry, microbiology,
physiology, pharmacology, and the medical sciences to name a few.
It has also led to the growth of the biotechnology industry, which
seeks to develop and commercialize many ofthese important processes
and methods. Much ofthis has come about because ofthe devel opment
of numerous molecular biology and genetic manipulation techniques.
The discovery of restriction enzymes and the development of cloning
vectors in the early
1970sopenedthedoortowaysofisolatingandmanipulatingnucleic
acidsthathadnever been thought possible. Gene probe labeling and
hybridization were developed and refined
toprovidepowerfulmethodsofanalysis.
These-togetherwiththedevelopment of DNA sequencing methods, protein
engineering techniques, and PeR-have all continued to contribute
substantially to the understandingofbiological processes at the
molecular level. Theprotocols for these importantmethods are the
focus ofThe Nucleic AcidProtocols Handbook, whose aim is to provide
a comprehensive set oftechniques in onevolume thatwill enable the
isolation, analysis, and manipulationofnucleic acids to be readily
undertaken. The NucleicAcidProtocols Handbook is divided into 10
parts; within each there are approximately 10chapters. The first
fourpartsfollow oneanotherlogically: nucleic acid extraction (Part
I), basic separation and analysisofDNA (II), through probe design
and labeling (III), and RNA analysis techniques (IV). The following
three sections deal with gene libraryconstruction andscreening(V),
DNA sequencing (VI), andthe polymerase chain reaction (VII)."
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