Given the vital importance of immune system research, the
gathering of clear, consistent, and informative protocols involving
the study of dendritic cells is paramount. Bringing the popular
first edition fully up to date, Dendritic Cell Protocols, Second
Edition presents protocols from experts in the field that cover the
basics and more complex forays into the exploration of DC
development and function, both in mice and humans. The first
section of the volume involving humans explores topics such as the
isolation of blood DC subtypes, primary skin Langerhans cells, and
the generation of gene-manipulated human DCs with the inclusion of
more clinically relevant methods as well, while the second section
involving rodent models delves into DC and precursor generation in
vitro, isolation ex vivo, disease models, as well as DC functions
and properties. Written in the highly successful Methods in
Molecular Biologya" series style, chapters include introductions to
their respective subjects, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Dendritic Cell Protocols, Second
Edition aims to become a bench-side handbook for both beginners and
experts in the field of DC research and a long-term reference for
some of the most popular methods put forward by those who lead the
field.
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