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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical)
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Hydrocarbon and Lipid Microbiology Protocols - Ultrastructure and Imaging (Paperback, Softcover reprint of the original 1st ed. 2016)
Loot Price: R3,575
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Hydrocarbon and Lipid Microbiology Protocols - Ultrastructure and Imaging (Paperback, Softcover reprint of the original 1st ed. 2016)
Series: Springer Protocols Handbooks
Expected to ship within 10 - 15 working days
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This Volume presents key microscopy and imaging methods for
revealing the structure and ultrastructure of environmental and
experimental samples, of microbial communities and cultures, and of
individual cells. Method adaptations that specifically address
problems concerning the hydrophobic components of samples are
highlighted and discussed. The methods described range from
electron microscopy and light and fluorescence microscopy, to
confocal laser-scanning microscopy, and include experimental
set-ups for the analysis of interfacial processes like microbial
growth and activities at hydrocarbon:water interfaces, biofilms and
microbe:mineral interfaces. Three forms of fluorescence in situ
hybridization - CARD-FISH, MAR-FISH and Two-pass TSA-FISH - are
described for the ecophysiological analysis of functionally active
microbes in samples. The methods presented will enable readers to
obtain an ultrastructural picture of, and identify the key
functional microbes in, samples under investigation. This in turn
will constitute a key framework for the interpretation of
information from other experimental approaches, such as
physicochemical analyses and genomic investigations. Hydrocarbon
and Lipid Microbiology ProtocolsThere are tens of thousands of
structurally different hydrocarbons, hydrocarbon derivatives and
lipids, and a wide array of these molecules are required for cells
to function. The global hydrocarbon cycle, which is largely driven
by microorganisms, has a major impact on our environment and
climate. Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
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