The dependence of present farming on artificial input of "chemical fertilizers" has caused numerous ecological tribulations associated with global warming and soil contamination. Moreover, there is an essential requirement for realistic agricultural practices on a comprehensive level. Accordingly, biofertilizers including microbes have been recommended as feasible environmentally sound solutions for agricultural practices which not only are natural, and cost-effective but also preserve soil environs and important biota of agricultural land. In addition, it enhances the nutrient quantity of soils organically. Microbial biofertilizers promote plant growth by escalating proficient absorption of nutrients for the plants and by providing an excellent disease-fighting mechanism.Agriculture, the backbone of human sustenance, has been put under tremendous pressure by the ever-increasing human population. Although various modern agro-techniques boosted agricultural production, the excessive use of synthetic fertilizers, pesticides and herbicides have proven extremely detrimental to agriculture as well as to the environment in which it is carried out. Besides this some faulty agricultural practices like monoculture and defective irrigation, further complicate the scenario by eliminating biodiversity, increasing the efflux of nutrients into the water bodies, the formation of algal blooms, eutrophication, damaging the water quality and lowering fish stocks. Biofertilizers are the organic compounds applied to crops for their sustainable growth and the sustainability of the environment as the microbiota associated with biofertilizers interact with the soil, roots and seeds to enhance soil fertility. Application of biofertilizers results in the increased mineral and water uptake, root development, vegetative growth and nitrogen fixation besides liberating growth-promoting substances and minerals that help the maintenance of soil fertility. They further act as antagonists and play a pivotal role in neutralising soil-borne plant pathogens and thus, help in the bio-control of diseases. Application of biofertilizers instead of synthetic fertilizers could be a promising technique to raise agricultural productivity without degrading environmental quality. The present book focuses on the latest research approaches and updates from the microbiota and their applications in the agriculture industry. We believe this book addresses various challenges and shed lights on the possible future of the sustainable agricultural system.

On the occasion of its twenty-fifth anniversary, in 1985, the Netherlands Society for Grassland and Fodder Crops (NVWV) agreed to organize an International Symposium on a topic related to intensive grass and fodder production systems. The theme selected was "Animal manure on grassland and fodder crops: Fertilizer or waste?" This Symposium was organized under the auspices of the European Grassland Federation and held at the International Agricultural Centre in Wageningen from 31 August to 3 September 1987. The problems connected with the disposal of animal waste have received much attention in recent years, especially in regions with intensive animal of animal manure per hectare agricul husbandry. Whereas the production tural land increased strongly, the need for it decreased because of the introduction of cheap inorganic fertilizers which are easier to handle and have a more reliable effect on crop growth. As a consequence, many farmers dispose of animal manure as cheaply as possible, whilst avoiding damage to grassland and crops and paying little attention to effective use of the plants nutrients contained in the manure. Present practices of manure handling and application often lead to environmental problems. The rise in awareness of these problems renewed interest in possibilities to improve the utilization of nutrients from animal manure in crop production. Research on this topic has been stimulated in many countries during the last decade and the aim of this Symposium was to review and assess present-day knowledge."

This book is the self-contained fourth volume of a seven-volume comprehensive series on nitrogen fixation. The outstanding aspect of this book is the integration of basic and applied work on biological nitrogen fixation in the fields of agriculture, forestry, and ecology in general. Nowadays, the concept of sustainability, which originated in agriculture and land use, is reaching many other areas of society and industry. Sustainability has a major part to play in the global challenge of continued development of regions, countries, and continents all around the World and biological nitrogen fixation has a key role in this process. This volume begins with chapters specifically addressing crops of major global importance, such as soybeans, rice, and sugar cane. It continues with a second important focus, agroforestry, and describes the use and promise of both legume trees with their rhizobial symbionts and other nitrogen-fixing trees with their actinorhizal colonization. An over-arching theme of all chapters is the interaction of the plants and trees with microbes and this theme allows other aspects of soil microbiology, such as interactions with arbuscular mycorrhizal fungi and the impact of soil-stress factors on biological nitrogen fixation, to be addressed. Furthermore, a link to basic science occurs through the inclusion of chapters describing the biogeochemically important nitrogen cycle and its key relationships among nitrogen fixation, nitrification, and denitrification. The volume then provides an up-to-date view of the production of microbial inocula, especially those for legume crops. No other available work provides the up-to-date and in-depth coverage of this volume, whichis intended to serve as an indispensable reference work for academic, government, and industrial scientists working in the applied areas of agronomy, plant breeding, plant nutrition, ecology, and forestry as well as those in the basic science areas of plant physiology, soil microbiology, and related environmental disciplines. This volume will be an invaluable tool for students entering this challenging area of research and will provide science administrators with ready access to vital relevant information.

The improvement of crop species has been a basic pursuit since cultivation began thousands of years ago. To feed an ever increasing world population will require a great increase in food production. Wheat, corn, rice, potato and few others are expected to lead as the most important crops in the world. Enormous efforts are made all over the world to document as well as use these resources. Everybody knows that the introgression of genes in wheat provided the foundation for the "Green Revolution". Later also demonstrated the great impact that genetic resources have on production. Several factors are contributing to high plant performance under different environmental conditions, therefore an effective and complementary use of all available technological tools and resources is needed to meet the challenge.

Presenting the state of the art of tissue culture and in vitro propagation of vegetable and tuber crops, medicinal and aromatic plants, fibre and oilseed crops, and grasses, this book complements the previous two volumes on High-Tech and Micropropagation, which concentrated on special techniques (Vol.17) and trees and bushes of commercial value (Vol.18). The specific plants covered here include asparagus, lettuce, horse radish, cucumber, potato, cassava, sweet potato, artichoke, yams, cardamom, fennel, celery, thyme, leek, mentha, turmeric, lavender, agave, yucca, cotton, jute, sunflower, ryegrass, zoysiagrass, and various species of "Aconitum," "Artemisia," "Camelia," "Centaurium," "Digitalis," "Dioscorea," "Glehnia," "Levisticum," "Parthenium," and "Pinella." The book is of use to advanced students, teachers and research workers in the field of pharmacy, horticulture, plant breeding and plant biotechnology in general, and also to individuals interested in industrial micropropagation.

1.1. INTRODUCTION Plastic covering, either framed or floating, is now used worldwide to protect crops from unfavorable growing conditions, such as severe weather and insects and birds. Protected cultivation in the broad sense, including mulching, has been widely spread by the innovation of plastic films. Paper, straw, and glass were the main materials used before the era of plastics. Utilization of plastics in agriculture started in the developed countries and is now spreading to the developing countries. Early utilization of plastic was in cold regions, and plastic was mainly used for protection from the cold. Now plastic is used also for protection from wind, insects and diseases. The use of covering techniques started with a simple system such as mulching, then row covers and small tunnels were developed, and finally plastic houses. Floating mulch was an exception to this sequence: it was introduced rather recently, although it is a simple structure. New development of functional and inexpensive films triggered widespread use of floating mulch. Table 1.1. The use a/plastic mulch in the world (after Jouet, 2001).

Growing concerns about the impacts of climate change and dependence on fossil fuels have intensified interest in bioenergy from sugar cane and other crops, highlighting important links between energy, environment and development goals. Sub-Saharan Africa is characterized by severe poverty; the possibility to exploit a renewable energy resource offers valuable avenues for sustainable development and could support a more dynamic and competitive economy. This book describes how the bioenergy expansion will improve rural livelihoods, reduce costly energy imports, reduce GHG emissions, and offer new development paths.

Drawing on international experience, it is shown that harnessing this potential will require significant increases in investment, technology transfer, and international cooperation. Because of its high efficiency, the authors argue that sugar cane should be viewed as a global resource for sustainable development and should command much greater focus and concerted policy action. Through an analysis of the agronomy, land suitability and industrial processing of sugar cane and its co-products, along with an assessment of the energy, economic and environmental implications, this volume demonstrates that sugar cane offers a competitive and environmentally beneficial resource for Africa's economic development and energy security.

With fourty-four authors representing thirty organisations in sixteen countries, the book offers a truly international and interdisciplinary perspective by combining technical and economic principles with social, political and environmental assessment and policy analysis.

Emphasis in agricultural production has shifted from mere quantity to quality products. Practical experience and scientific investigations have shown that, of the various culture measures, balanced fertilization above all exerts a considerable influence on the quality of agricultural products. Simply adding more of what the crop has already absorbed to capacity is unproductive, expensive, wasteful and damaging to the environment. Therefore, balanced crop nutrition increases crop quality, safeguards natural resources and brings benefit to the farmer. Otherwise rapid population growth and severe urbanization will exhaust our natural resources.

The rapidly growing human population has increased the dependence on fossil fuel based agrochemicals such as fertilizers and pesticides to produce the required agricultural and forestry products. This has exerted a great pressure on the non renewable fossil fuel resources, which cannot last indefinitely. Besides, indiscriminate use ofpesticides for pests (weeds, insects, nematodes, pathogens) control has resulted in serious ecological and environmental problems viz., (A) Increasing incidence of resistance in pest organisms to important pesticides. (B) Shift in pests population, particulary in weeds and insects. In weeds, species that are more closely related to the crops they infest have developed. In insects, scenario is most grim, the predators have been killed and minor insect pests have become major pests and require very heavy doses ofhighly toxic insecticides for their control. (C) Greater environmental pollution and health hazards (a) particularly from contamination of surface and underground drinking water resources and (b) from their inhalation during handling and application. (D) Toxic residues of pesticides pollute the environment and may prove hazardous to even our future generations. (E) Some agricultural commodities may contain minute quantities ofpesticides residues, with long tenn adverse effects on human and livestock health. Therefore, serious ecological questions about the reliance on pesticides for pests control has been raised. The use of fertilizers, besides causing environmental problems has also impoverished the soil health and decreased the beneficial soil fauna. For example, in some major crop rotations viz."

Medicinal Plants, Volume 6 of the Genetic Resources, Chromosome Engineering, and Crop Improvement series summarizes landmark research and describes medicinal plants as nature's pharmacy. Highlights Examines the use of molecular technology for maintaining authenticity and quality of plant-based products Details reports on individual medicinal plants including their history, origin, genetic resources, cytogenetics, and varietal improvement through conventional and modern methods, and their use in pharmaceutical, cosmeceutical, nutrition, and food industries Explains how to protect plants with medicinal properties from deforestation, urbanization, overgrazing, pollution, overharvesting, and biopiracy Brings together information on germplasm resources of medicinal plants, their history, taxonomy and biogeography, ecology and biodiversity, genetics and breeding, exploitation, and utilization in the medicine and food industries Written by leading international experts and an innovative panel of scientists, Medicinal Plants offers the most comprehensive and up-to-date information on medicinal plant genetic resources and their increasing importance in pharmaceutical and cosmeceutical industries, medicine, and nutrition around the world. Includes eight-page color insert more than 25 full color figures.

In recent years there has been an unprecedented expansion of knowledge about anthocyanins pigments. Indeed, the molecular genetic control of anthocyanins biosynthesis is now one of the best understood of all secondary metabolic pathways. There have also been substantial improvements in analytical technology that have led to the discovery of novel anthocyanin compounds. Armed with this knowledge and the tools for genetic engineering, plant breeders are now introducing vibrant new colors into horticultural crops. The food industry has also benefited from the resurgence of interest in anthocyanins. A greater understanding of the chemistry of these pigments has led to improved methods for stabilizing the color of anthocyanins extracts, so that they are more useful as food colorings. Methods for the bulk production of anthocyanins from cell cultures have been optimized for this purpose. Possible benefits to human health from the ingestion of anthocyanin-rich foods have also been a major feature of the recent scientific literature. Anthocyanins are remarkably potent antioxidants, and their ingestion has been postulated to stave off the effects of oxidative stress. These pigments, especially in conjunction with other flavonoids, have been associated with reductions in the incidence and severity of many other non-infectious diseases, including diabetes, cardiovascular disease and certain cancers. An industry is developing around anthocyanins as nutritional supplements. Finally, there has been significant progress in our understanding of the benefits of anthocyanins to plants themselves. Originally considered an extravagance without a purpose, anthocyanins are now implicated in multifarious vital functions. These include the attraction of pollinators and frugivores, aposematic defense from herbivores, and protection from environmental stressors such as strong light, UVB, drought, and free radical attacks. Anthocyanins are evidently highly versatile, and enormously useful to plants. This book covers all aspects of the biosynthesis and function of anthocyanins (and related compounds such as proanthocyanidins) in plants, and their applications in agriculture, food products, and human health. Featured areas include their relevance to: * Plant stress * Flower and fruit color * Human health * Wine quality and health attributes * Food colorants and ingredients * Cell culture production systems * The pastoral sector

This book addresses the impact of important climatic changes on plant pests (including weeds, diseases and insect pests), and their interactions with crop plants. Anthropogenic activities have seriously impacted the global climate. As a result, carbon dioxide (CO2) and temperature levels of the earth are on a continuous rise. The global temperature is expected to increase by a 3 DegreesC or more by the end of this century. The CO2 concentration was below 300 parts per million (ppm) before the start of the industrial era; however, recently it has exceeded 400 ppm. This is highest ever in human history. Other than global warming and elevated CO2 concentrations, anthropogenic activities have also disturbed the global water cycle, ultimately, impacting the quantity and distribution of rainfall. This has resulted in drought conditions in many parts of the world. Global warming, elevated CO2 concentration and drought are considered the most important recent climatic changes that are impacting global ecosystems and human societies. Among other impacts, the effects of climatic changes on pests, pest-crop interactions and pest control are important with relevance to global food security, and hence require immediate attention by plant scientists. This book discusses innovative and the most effective pest control methods under an environment of changing climate and elaborates on the impact of drought on plant pests and their control.

Genome Mapping and Molecular Breeding in Plants presents the current status of the elucidation and improvement of plant genomes of economic interest. The focus is on genetic and physical mapping, positioning, cloning, monitoring of desirable genes by molecular breeding and the most recent advances in genomics. The series comprises seven volumes: Cereals and Millets; Oilseeds; Pulses, Sugar and Tuber Crops; Fruits and Nuts; Vegetables; Technical Crops; and Forest Trees.

Technical Crops includes plants of great agricultural importance. One chapter is devoted to cotton, the most important fiber crop on which significant progress in molecular genetic research has been made. Reviews on oil palm, coffee, tea, cocoa and rubber describe traditional breeding and preliminary molecular results. Chapters on forage crops, ornamentals, and medicinal and aromatic plants each cover a large number of crops and may serve as road maps for further molecular research.

Phosphorus (P) is an essential macronutrient for plant growth. It is as phosphate that plants take up P from the soil solution. Since little phosphate is available to plants in most soils, plants have evolved a range of mechanisms to acquire and use P efficiently - including the development of symbiotic relationships that help them access sources of phosphorus beyond the plant's own range. At the same time, in agricultural systems, applications of inorganic phosphate fertilizers aimed at overcoming phosphate limitation are unsustainable and can cause pollution.

This latest volume in Springer's Plant Ecophysiology series takes an in-depth look at these diverse plant-phosphorus interactions in natural and agricultural environments, presenting a series of critical reviews on the current status of research. In particular, the book presents a wealth of information on the genetic and phenotypic variation in natural plant ecosystems adapted to low P availability, which could be of particular relevance to developing new crop varieties with enhanced abilities to grow under P-limiting conditions.

The book provides a valuable reference material for graduates and research scientists working in the field of plant-phosphorus interactions, as well as for those working in plant breeding and sustainable agricultural development.

Cold stress is one of the prevalent environmental stresses affecting crop productivity, particularly in temperate regions. Numerous plant types of tropical or subtropical origin are injured or killed by non-freezing low temperature, and display a range of symptoms of chilling injury such as chlorosis, necrosis, or growth retardation. In contrast, chilling tolerant species thrive well at such temperatures. To thrive under cold stress conditions, plants have evolved complex mechanisms to identify peripheral signals that allow them to counter varying environmental conditions. These mechanisms include stress perception, signal transduction, transcriptional activation of stress-responsive target genes, and synthesis of stress-related proteins and other molecules, which help plants to strive through adverse environmental conditions. Conventional breeding methods have met with limited success in improving the cold tolerance of important crop plants through inter-specific or inter-generic hybridization. A better understanding of physiological, biochemical and molecular responses and tolerance mechanisms, and discovery of novel stress-responsive pathways and genes may contribute to efficient engineering strategies that enhance cold stress tolerance. It is therefore imperative to accelerate the efforts to unravel the biochemical, physiological and molecular mechanisms underlying cold stress tolerance in plants. Through this new book, we intend to integrate the contributions from plant scientists targeting cold stress tolerance mechanisms using physiological, biochemical, molecular, structural and systems biology approaches. It is hoped that this collection will serve as a reference source for those who are interested in or are actively engaged in cold stress research.

Proceedings of an International Symposium

Plant nutrition in greenhouse cultivation differs in many essential aspects from field crops and justified the development of a special publication on this subject. The high productions realised and the specific produce quality requirements ensure high uptakes of nutrients and a careful tuning of the application. The covering with glass or plastic is responsible for specific climatic conditions, which in modern greenhouse can be fully adjusted to the requirements of the crop by automatic climate control. The natural precipitation is excluded, thus, the water has to be applied in greenhouses by artificial irrigation of water from different origin. On thing and another involves that the growing conditions are more or less completely controlled. This especially holds when the crops are grown in substrates.The high uptake of minerals in greenhouses requires high fertilizer additions. The quantities absorbed by many crops are that high, that it is impossible to supply the required quantities of nutrients as a base dressing at once. Therefore, top dressings are common practice and are carried out together with the supply of the irrigation water. Therefore, fertigation is common practice and in greenhouses already for many years. Specific systems have been developed for the application of the right concentrations to keep the level of nutrients in the root environment on the optimum level for the performance of the crop.Beside the management of the nutrient application, greenhouse growers also need a close control on the salt accumulation. This accumulation is closely connected with the quality of the irrigation water. Moreover, also the addition of the fertilizers plays a role in the salt accumulation in the root environment. Therefore, choice of the fertilizers used is important to prevent accumulations of residual salts possibly supplied with the fertilizers. On the other hand, for a number of crops the level of fertilizer supply is not only focussed on the nutrient requirements, but also utilized to realize a certain salt concentration in the irrigation water. In this way the osmotic potential of the soil solution is affected and this characteristic is an important tool for the grower for the regulation of the growth of the crop and the quality of the produce. When the salinity passes certain threshold values, the growth and production of crops is reduced, but the quality of the harvested produce of some crops is improved. Such regulations are very precisely adjusted to the crops grown and to the growing conditions in the greenhouse. Another line is the development of sustainable production methods. For the main subject discussed in this book, namely plant nutrition, methods for an optimum use of fertilizers with a minimum environmental pollution were developed last decennia. In this field the development of the cultivation in substrates offered excellent possibilities for an optimal use of water and nutrients. With this growing method it has been proved that it is possible to grow greenhouse crops without any discharge of minerals to the environment. The conditions required for such cultivation are thoroughly discussed. Moreover, growing in substrates offers suitable opportunities for optimization of yield and quality, because of the adequate control on the conditions in the root environment, like the supply of water and nutrients. However, this requires a perfect management of water and nutrient supply. Not only for the fact that plants are grown in very small rooting volumes and therefore, mistakes with irrigation and fertilizer supply easily will damage the crop, but also for the fact that the fertilizer supply is complicated. For substrate growing it is not enough that some nutrient elements are controlled, like with soil grown crops, but the full packet of nutrients essential for plant growth will be kept in view. This means that the addition of six macro nutrients and at least six micro nutrients will be regulated, with respect to the right concentration and mutual ratios in the irrigation water. Physical and chemical properties of substrates essentially differ and a right use of these properties is necessary for a right interpretation of the nutrient and salinity status. To this purpose the grower is supplied with detailed recommendations developed by the research stations founded in The Netherlands. The horticultural research stations in The Netherlands developed numerous tools to the growers often in cooperation with the horticultural industries and laboratories. An example of such cooperation is the development of soil and substrate testing methods by the research stations. These methods offered excellent possibilities for a frequent control for the salt and nutrient status in the root environment. Together with these methods schedules for interpretation and recommendation were developed and adjusted for computerized information to the growers.
The book contains information about soil testing methods, in relation to a universal interpretation based on the composition of soil solution. Methods for interpretations of tissue tests are supplied. Crop response on salinity and water supply is discussed in relation to fertilizer application. The management of fertilizer addition in relation to analytical data of soil and substrate samples is presented for a wide range of crops grown in greenhouses. The specific requirements in relation with the climatic conditions and the crop grown are discussed.
The management as described is especially focussed on a sustainable production of vegetable and ornamental crops. The water supply, fertilization and the realisation of the osmotic potential in the root environment is focussed on the production of the high quality products required on the consumer market as appeared last decennia in the Western world. Such a market is characterized by diversity, quality and immediate answers to demands of luxurious productions. Greenhouse production

This book, written by leading grain scientists from Europe and Africa, examines six such grains that have been important food crops in various parts of the world and have the potential for much greater and more widespread use. The chemistry, nutritional value, food processing technologies and potential applications of three true cereals: sorghum, spelt wheat and the major millet species, and three dicotyledonous pseudocereals: grain amaranth, buckwheat and quinoa are discussed. Just three cereal grains account for more than 75% of all grains produced worldwide. This causes high risks for the future of humankind via catastrophic food crop failures and is detrimental to our long-term health. In addition, the intensive cultivation practices needed to produce the required high yields of these cereals is frequently leading to environmental degradation, and they are often inappropriate in the Developing World.

While working in the laboratory of Professor Dr. Jacob Reinert at the Freie Universitat Berlin (1974-1976), I had the opportunity to become deeply involved in studying the intricacies of the fascinating phenomenon of somatic embryogenesis in plant cells and protoplasts. In numerous stimu lating discussions with Professor Reinert on this subject, I was fully convinced that somatic embryogenesis would become one of the most important areas of study, not only regarding basic and fundamental aspects, but also for its application in crop improvement. During the last decade, we have witnessed tremendous interest and achievements in the use of somatic embryos for the production of synthetic seeds, for micro propagation, genetic transformation, cryopreservation, and conservation of germplasm. The en masse production of somatic embryos in the bioreactors has facilitated some of these studies. Somatic embryos have now been induced in more than 300 plant species belonging to a wide range offamilies. It was therefore felt that a compilation ofliterature/state of the art on this subject was necessary. Thus, two volumes on Somatic Embryo genesis and Synthetic Seed have been compiled, which contain 65 chapters contributed by International experts. Somatic Embryogenesis and Synthetic Seed I comprises 31 chapters, arranged in 3 sections: Section I Commitment of the cell to somatic embryogenesis; early events; anatomy; molecular basis; gene expression; role of polyamines; machine vision analysis of somatic embryos. Section II Applications of somatic embryos; technology of synthetic seed; fluid drilling; micropropagation; genetic transfor mation through somatic embryos; cryopreservation.

This book contains papers and posters presented at the 18th Eucarpia Fodder Crops Section Meeting held at Loen, Nordfjord, Norway in August 1993. In most environments some form of marginal conditions or stress prevails. Few crops are being produced under such a wide range of environmental and management stresses as fodder crops. Improved adaptation of fodder crops to marginal conditions is crucial in developing sustainable, low-input agricultural systems. The book is unique in demonstrating the large diversity both in crops and environmental stresses that confront the forage breeders. Both general and specific aspects of adaptation to marginal growing conditions are presented, ranging from problems caused by snow and ice in the Subarctic regions of Europe to the severe drought problems in the Mediterranean regions. For everyone involved in studies of adaptation and breeding of perennial plants for marginal conditions or stress environments.

Incorporating contributions from microbiologists, molecular biologists, plant breeders and soil scientists this volume reports the results and recommendations of an FAO/IAEA meeting of twelve experts on biological nitrogen fixation. This volume will be invaluable to scientists working on nitrogen fixation, soil microbiology, agronomy and crop production as well as farm advisers and extension specialists.
Maximising the Use of Biological Nitrogen Fixation in Agriculture is unique in that it:

-reviews the latest thinking on various aspects of biological nitrogen fixation technology and applications;
-reviews the possibilities in enhancing nitrogen fixation in various cropping systems;
-shows ways how biological nitrogen fixation can be used to enhance crop production;
-considers the applicability of these technologies to small farmers in developing countries.

Genome Mapping and Molecular Breeding in Plants presents the current status of the elucidation and improvement of plant genomes of economic interest. The focus is on genetic and physical mapping, positioning, cloning, monitoring of desirable genes by molecular breeding and the most recent advances in genomics. The series comprises seven volumes: Cereals and Millets; Oilseeds; Pulses, Sugar and Tuber Crops; Fruits and Nuts; Vegetables; Technical Crops; and Forest Trees.

Cereals and Millets form the leading group of field crops, providing staple food for most of the earth s population. This volume, with contributions by 27 eminent scientists, includes chapters on rice, wheat, maize, barley, oats, rye, sorghum, pearl millet, foxtail millet and finger millet. The emphasis is on advanced research on the major crops, including the model plants maize and rice, as well as on future road maps of genomic research for the less-often considered but equally deserving cereals and millets. "

Genome Mapping and Molecular Breeding in Plants presents the current status of the elucidation and improvement of plant genomes of economic interest. The focus is on genetic and physical mapping, positioning, cloning, monitoring of desirable genes by molecular breeding and the most recent advances in genomics. The series comprises seven volumes: Cereals and Millets; Oilseeds; Pulses, Sugar and Tuber Crops; Fruits and Nuts; Vegetables; Technical Crops; and Forest Trees.

Fruits and nuts form the largest group among crop plants. Several constraints such as long life cycle, heterozygosity and large plant size caused comparatively slow research progress in the past. The chapters on 20 fruit and nut crops authored by 56 renowned scientists from 12 countries include for the first time comprehensive reviews on mango, banana, olive, pineapple, pistachio, persimmon and papaya. Other crops covered are apple, grape, cherry, plum, peach, pear, apricot, strawberry, raspberry, blueberry, almond, citrus and avocado.

Fantasies and dreams have their rightful place in science, and sometimes they turn into reality. Regeneration of hybrid plants through protoplast fusion is one such dream come true. In the early 1970s I shared the pioneering excitement in the field of protoplast technology at the Second International Congress of Plant Tissue Culture held in Strasbourg, France. Subsequently, I participated in three international conferences devoted to plant protoplasts, in Salamanca, Spain (1972), Versailles, France (1972), and Nottingham, England (1975). At Versailles Dr. P.S. Carlson presented his work on the successful regeneration of somatic hybrids between Nicotiana glauca and Nicotiana langsdorfii. The enthusi- asm shown by the participants was sufficient indication of the bright future of somatic hybridization. On my return from Versailles, I gathered my thoughts and prepared a concept paper on Potentials of Protoplast Culture Work in Agriculture which was published in Euphytica (Bajaj 1974). The studies on protoplast fusion and somatic hybridization then gained momentum and active work started in many laboratories. Very significant work was done by Melchers et al. (1978) who obtained a somatic hybrid between potato and tomato, calling it "Pomato".

In recent years, significant advancements have been made in the management of nutritional deficiency using genome engineering--enriching the nutritional properties of agricultural and horticultural crop plants such as wheat, rice, potatoes, grapes, and bananas. To meet the demands of the rapidly growing world population, researchers are developing a range of new genome engineering tools and strategies, from increasing the nutraceuticals in cereals and fruits, to decreasing the anti-nutrients in crop plants to improve the bioavailability of minerals and vitamins. Genome Engineering for Crop Improvement provides an up-to-date view of the use of genome editing for crop bio-fortification, improved bioavailability of minerals and nutrients, and enhanced hypo-allergenicity and hypo-immunogenicity. This volume examines a diversity of important topics including mineral and nutrient localization, metabolic engineering of carotenoids and flavonoids, genome engineering of zero calorie potatoes and allergen-free grains, engineering for stress resistance in crop plants, and more. Helping readers deepen their knowledge of the application of genome engineering in crop improvement, this book: Presents genetic engineering methods for developing edible oil crops, mineral translocation in grains, increased flavonoids in tomatoes, and cereals with enriched iron bioavailability Describes current genome engineering methods and the distribution of nutritional and mineral composition in important crop plants Offers perspectives on emerging technologies and the future of genome engineering in agriculture Genome Engineering for Crop Improvement is an essential resource for academics, scientists, researchers, agriculturalists, and students of plant molecular biology, system biology, plant biotechnology, and functional genomics.