This book contains information on plant-based nutrients and phytochemicals in vegetable crops that are commonly consumed. The information generated by researchers using modern methods of biochemical analysis and results, mostly validated using cell line cultures or animal models, and to a limited extent on human volunteers have been presented. The vegetable crops have been grouped based on the family they belong to, because most often members of the same family have similar biomolecules as active ingredients and have similar effects on health. The book will help students/researchers/scientists and common man alike to look at the vegetables as protective foods, not just because it is said so, but with a scientific explanation. Note: T&F does not sell or distribute the hardback in India, Pakistan, Nepal, Bhutan, Bangladesh and Sri Lanka. This title is co-published with NIPA.

The Handbook of Research on Food Processing and Preservation Technologies covers a vast abundance of information on various design, development, and applications of novel and innovative strategies for food processing and preservation. The roles and applications of minimal processing techniques (such as ozone treatment, vacuum drying, osmotic dehydration, dense phase carbon dioxide treatment, pulsed electric field, and high-pressure assisted freezing) are discussed, along with a wide range of applications. The handbook also explores some exciting computer-aided techniques emerging in the food processing sector, such as robotics, radio frequency identification (RFID), three-dimensional food printing, artificial intelligence, etc. Some emphasis has also been given on nondestructive quality evaluation techniques (such as image processing, terahertz spectroscopy imaging technique, near infrared, Fourier transform infrared spectroscopy technique, etc.) for food quality and safety evaluation. The significant roles of food properties in the design of specific foods and edible films have been elucidated as well. Volume 5: Emerging Techniques for Food Processing, Quality, and Safety Assurance discusses various emerging techniques for food preservation, formulation, and nondestructive quality evaluation techniques. Each chapter covers major aspects pertaining to principles, design, and applications of various food processing methods, such as low temperature-based-ultrasonic drying of foods, hypobaric processing of foods, viability of high-pressure technology, application of pulsed electric fields in food preservation, green nanotechnology for food processing and preservation, advanced methods of encapsulation, basics and methods of food authentication, imaging techniques for quality inspection of spices and nuts, FTIR coupled with chemometrics for food quality and safety, and the use of robotic engineering for quality and safety. Other volumes in the 5-volume set include: Volume 1: Nonthermal and Innovative Food Processing Methods Volume 2: Nonthermal Food Preservation and Novel Processing Strategies Volume 3: Computer-Aided Food Processing and Quality Evaluation Techniques Volume 4: Design and Development of Specific Foods, Packaging Systems, and Food Safety Together with the other volumes in the set, the Handbook of Research on Food Processing and Preservation Technologies will be a valuable resource for researchers, scientists, students, growers, traders, processors, industries, and others.

The Handbook of Research on Food Processing and Preservation Technologies is a valuable 5-volume collection that illustrates various design, development, and applications of novel and innovative strategies for food processing and preservation. The roles and applications of minimal processing techniques (such as ozone treatment, vacuum drying, osmotic dehydration, dense phase carbon dioxide treatment, pulsed electric field, and high-pressure assisted freezing) are discussed, along with a wide range of applications. The handbook also explores some exciting computer-aided techniques emerging in the food processing sector, such as robotics, radio frequency identification (RFID), three-dimensional food printing, artificial intelligence, etc. Some emphasis has also been given on nondestructive quality evaluation techniques (such as image processing, terahertz spectroscopy imaging technique, near infrared, Fourier transform infrared spectroscopy technique, etc.) for food quality and safety evaluation. The significant roles of food properties in the design of specific foods and edible films have been elucidated as well. Volume 4: Design and Development of Specific Foods, Packaging Systems, and Food Safety presents new research on health food formulation, advanced packaging systems, and toxicological studies for food safety. This volume covers in detail the design of functional foods for beneficial gut microflora, design of specific foods for gut microbiota, composite probiotic dairy products: concepts and design with a focus on millets, encapsulation technology for development of specific foods, prospects of edible and alternative food packaging technologies, recent advancements in edible and biodegradable materials for food packaging, potential of ozonation in surface modification of food packaging polymers, characterization applications and safety aspects of nanomaterials used in food and dairy industry, toxic effects of tinplate corrosion, and mitigation measures in canned foods. Other volumes in the set include: Volume 1: Nonthermal and Innovative Food Processing Methods Volume 2: Nonthermal Food Preservation and Novel Processing Strategies Volume 3: Computer-Aided Food Processing and Quality Evaluation Techniques Volume 5: Emerging Techniques for Food Processing, Quality, and Safety Assurance The book helps to provide an understanding of different food formulations and development of edible packaging techniques with emphasis on the assessment of food product safety and quality. The book also provides information on various methods of formulation for development of new and safe products. Together with the other volumes in the set, Handbook of Research on Food Processing and Preservation Technologies will be a valuable resource for researchers, scientists, students, growers, traders, processors, industries, and others.

The content of this book provides information on advanced knowledge in the sphere of importance and scope of horticulture in India, horticulture based integrated farming systems, integration of livestock in horticulture based farming systems, emerging issues, natural resource management, disease and pest management, organic farming and certification, post-harvest measures and value addition in arid fruits and vegetables, marketing aspects, status and export promotion measures and procedures. Note: T&F does not sell or distribute the hardback in India, Pakistan, Nepal, Bhutan, Bangladesh and Sri Lanka. This title is co-published with NIPA.

Analytical Techniques in Meat Science is a comprehensive compilation of all the relevant methodologies for the quality analysis of meat. The content of the book is designed to cater to requirement of meat producers, regulatory agencies, researchers, students, teachers, laboratory staff etc. It covers techniques for physico-chemical analysis, species identification and microbiological examination of meat. Also, it contains the latest biotechnological and proteomic techniques for meat quality evaluation. To help the reader understand better figures, tables, line diagrams, etc are used frequently whenever needed. Some important pictures are given in plates for lucid and clear understanding of the concept. Note: T&F does not sell or distribute the hardback in India, Pakistan, Nepal, Bhutan, Bangladesh and Sri Lanka.

Considering the ever-increasing global population and finite arable land, technology and sustainable agricultural practices are required to improve crop yield. This book examines the interaction between plants and microbes and considers the use of advanced techniques such as genetic engineering, revolutionary gene editing technologies, and their applications to understand how plants and microbes help or harm each other at the molecular level. Understanding plant-microbe interactions and related gene editing technologies will provide new possibilities for sustainable agriculture. The book will be extremely useful for researchers working in the fields of plant science, molecular plant biology, plant-microbe interactions, plant engineering technology, agricultural microbiology, and related fields. It will be useful for upper-level students and instructors specifically in the field of biotechnology, microbiology, biochemistry, and agricultural science. Features: Examines the most advanced approaches for genetic engineering of agriculture (CRISPR, TALAN, ZFN, etc.). Discusses the microbiological control of various plant diseases. Explores future perspectives for research in microbiological plant science. Plant-Microbial Interactions and Smart Agricultural Biotechnology will serve as a useful source of cutting-edge information for researchers and innovative professionals, as well as upper-level undergraduate and graduate students taking related agriculture and environmental science courses.

Chitin is one of the most important biopolymers, synthesized by an enormous number of living organisms and is a promising bioactive polymer for food packaging applications due to its functional properties. This book focuses on composition, properties, characterization, and theoretical approach of chitin and chitosan bio-composites. It describes the most recent studies concerning chitin and chitosan-based films and gives an overview about future trends regarding the industrial applications of chitin and chitosan for food packaging purposes. This book is especially useful for researchers in the fields of bionanocomposites, especially those with an interest in packaging applications.

Recent regulations on heavy metal testing have required the pharmaceutical industry to monitor a suite of elemental impurities in pharmaceutical raw materials, drug products and dietary supplements. These new directives s are described in the new United States Pharmacopeia (USP) Chapters <232>, <233>, and <2232>, together with Q3D, Step 4 guidelines for elemental impurities, drafted by the ICH (International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use), a consortium of global pharmaceutical associations, including the European Pharmacopeia (Ph.Eur.), the Japanese Pharmacopeia (JP) and the USP. This book provides a complete guide to the analytical methodology, instrumental techniques and sample preparation procedures used for measuring elemental impurities in pharmaceutical and nutraceutical materials. It offers readers the tools to better understand plasma spectrochemistry to optimize detection capability for the full suite of elemental PDE (Permitted Daily Exposure) levels in the various drug delivery categories. Other relevant information covered in the book includes: The complete guide to measuring elemental impurities in pharmaceutical and nutraceutical materials. Covers heavy metals testing in the pharmaceutical industry from an historical perspective. Gives an overview of current USP Chapters <232> <233> and <2232> and ICH Q3D Step 4 Guidelines. Explains the purpose of validation protocols used in Chapter <233>, including how J-values are calculated Describes fundamental principles and practical capabilities of ICP-MS and ICP-OES. Offers guidelines about the optimum strategy for risk assessment Provides tips on how best to prepare and present your data for regulatory inspection. An indispensable resource, the fundamental principles and practical benefits of ICP-OES and ICP-MS are covered in a reader-friendly format that a novice, who is carrying out elemental impurities testing in the pharmaceutical and nutraceutical communities, will find easy to understand.

This book provides a critical overview of analytical methods used for the determination of pesticide residues and other contaminants in food and environmental samples by modern instrumental analysis. It contains up-to-date material including recent trends in sample preparation, general methods used for pesticide analysis and quality assurance aspects, and chromatographic and immunoassay methods. The rest of the book describes particular analytical methods used for the determination of pesticides in food and soil, water and air. In addition, the levels of these chemicals found in food, their regulatory aspects and the monitoring of pesticides in the environment are described.

This new volume presents original research and scientific advances in the field of the food bioprocessing, bioproducts, valorization of agricultural and food wastes, microbiology, and biotechnology. It explores the most important advances in the valorization of agri-food residues for the production of bioproducts and in the development of several bioprocessing strategies. The authors place a special emphasis on the challenges that the industry faces in the era of sustainable development and aim to facilitate the reduction of food loss and waste. This book demonstrates the potential and actual development and advances in the design and development of strategies and tools for the bioprocessing of agri-food residues for the production of bioproducts. Bioprocessing of Agri-Food Residues for Production of Bioproducts covers aspects related to biotransformation of agri-food residues such as mango seed, citrus waste, pomegranate husks, nut shells, melon peels, leaves and grains, cheese whey, among others.

Covers the fundamental instrumentation and techniques Discusses HRMS-based phytochemical research details Focuses strictly on the phytochemical considerations

1.Covers important fields of algae right from biomass production to genetic engineering aspects of algae. 2.Useful for people doing research Algal biotechnology, Aquaculture, Marine micro and Macrobiology, Microbial biotechnology, Bioprocess technology 3. Focuses on therapeutic and nutritional areas of algae

Global food losses are a result of a lack of necessary infrastructure, improper food safety handling procedures, and insufficient training for the personnel working in the cold chain. The development of a resource-efficient and energy-smart food supply chain requires a well-integrated evaluation and development of the cold chain. Cold Chain Management for the Fresh Produce Industry in the Developing World provides a comprehensive review of the benefits of an unbroken cold chain in developing countries and focuses on the critical role of extension education in the implementation of cold chain management. The unbroken cold chain is essential for all stakeholders in the fresh produce industry to maintain the quality and safety of food products during handling, transporting, and storing in their journey from producer to consumer. Appropriate cold chain management is crucial not only to reduce the postharvest losses and wastages, but also to increase farmers' income, generate employment opportunities, and improve the livelihood of stakeholders along the supply chain. Key Features: Includes case studies for promoting the expansion of existing technologies for cold chain development in Asian, Africa and the Caribbean nations. Assesses cold chain management as crucial to the growth of global trade in perishable products with contributions from international organizations, researchers and commercial experts. Articulates resilient, sustainable and creative concepts to develop cold chains to enhance food distribution. This book comprises of chapters contributed by the experts and practitioners of cold chain development in developing countries. The authors in the book provide the scenario of cold chain management in the world and discuss the importance of the cold chain as well as the different options and innovations of cooling systems. Chapters also include case studies, success stories, capacity building activities, and other opportunities in cold chain development.

Due to its high sensitivity and selectivity, liquid chromatography-mass spectrometry (LC-MS) is a powerful technique. It is used for various applications, often involving the detection and identification of chemicals in a complex mixture. Ultra Performance Liquid Chromatography Mass Spectrometry: Evaluation and Applications in Food Analysis presents a unique collection of up-to-date UPLC-MS/MS methods for the separation and quantitative determination of components, contaminants, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. The book begins with an overview of the history, principles, and advancement of chromatography. It discusses the use of UHPLC techniques in food metablomics, approaches for analysis of foodborne carcinogens, and details of UPLC-MS techniques used for the separation and determination of capsaicinoids. Chapters describe the analysis of contaminants in food, including pesticides, aflatoxin, perfluorochemicals, and acrylamide, as well as potentially carcinogenic heterocyclic amines in cooked foods. The book covers food analysis for beneficial compounds, such as the determination of folate, vitamin content analysis, applications for avocado metabolite studies, virgin olive oil component analysis, lactose determination in milk, and analysis of minor components of cocoa and phenolic compounds in fruits and vegetables. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.

Animal products are good source of disposable income for many small farmers in developing countries. In fact, livestock are often the most important cash crop in many small holder mixed farming systems. Livestock ownership currently supports and sustains the livelihoods of rural poor, who depend partially or fully on livestock for their income and/or subsistence. Human population growth, increasing urbanization and rising incomes are predicted to double the demand for, and production of, livestock products in the developing countries over the next twenty years. The future holds great opportunities for animal production in developing countries. Animal Sourced Foods for Developing Economies addresses five major issues: 1) Food safety and nutritional status in developing world; 2) the contribution of animal origin foods in human health; 3) Production processes of animal foods along with their preservation strategies; 4) functional outcomes of animal derived foods; and finally, 5) strategies, issues and polices to promote animal origin food consumption. Animal sourced food contain high biological value protein and important micronutrients required for optimal body functioning but are regarded as sources of fat that contribute to the intake of total and saturated fatty acids in diet. The quality of protein source has a direct influence on protein digestibility, as a greater proportion of higher quality proteins is absorbed and becomes available for bodily functions. Animal foods has high quantity and quality of protein that includes a full complement of the essential amino acids in the right proportion. Land availability limits the expansion of livestock numbers in extensive production systems in most regions, and the bulk of the increase in livestock production will come from increased productivity through intensification and a wider adoption of existing and new production and marketing technologies. The significant changes in the global consumption and demand for animal source foods, along with increasing pressures on resources, are having some important implications for the principal production systems. In this book, contributors critically analyze and describe different aspects of animal's origin foods. Each chapter is dedicated to a specific type of food from animal source, its nutritional significance, preservation techniques, processed products, safety and quality aspects on conceptual framework. Special attention is given to explain current food safety scenario in developing countries and contribution of animal derived food in their dietary intake. Existing challenges regarding production, processing and promotion of animal's origin foods are also addressed with possible solutions and strengthening approaches.

Provides up to date fundamental information about probiotics Reveals the recent applications of probiotics in food and feed industries Discusses in depth the role of microbiota in human and animal wellness Comprises chapters by international experts in the field of probiotics

Aging can be perceived differently during different times in one's life. Aging as a process not only influences medical and economic dimensions at an individual level but also at societal and national levels. Aging is a natural process; however, its standard definition in a healthcare context is yet unclear. To delay the aging process and to maintain quality of life until the end of life are two goals of prime importance. Various healthcare approaches are being developed and experimented on to best manage aging as if it is a disease. Nutraceuticals are value-added dietary supplement products and have an immense potential in altering key structures and functions of aging. Nutraceuticals can be a keystone in altering sub-normal performing physiological and metabolic systems due to aging. Nutraceuticals for Aging and Anti-Aging: Basic Understanding and Clinical Evidence addresses aging and anti-aging nutraceuticals based on 10 major challenges, such as cognitive health, malnutrition, substance abuse, bladder control, and oral health, among others. It examines how these challenges can be complemented with nutraceuticals and connects the applications with the traditional wisdom of the aging process. Key Features Examines the aging process, then recommends nutraceuticals for aging and anti-aging processes Describes the aging process from the western perspective, and Ayurvedic medicine (Indian traditional system) and traditional Chinese medicine perspectives Provides, whenever possible, the clinical evidence of the applications of nutraceuticals for aging and anti-aging This book is a valuable resource for physicians, clinical experts, pharmaceutical companies and their experts, nutrition specialists, entrepreneurs, chemists, pharmacists, food chemists-technologists, as well as researchers and post-graduate students involved in these specialties. Also available in the Nutraceuticals: Basic Research/Clinical Applications Series: Bioactive Peptides: Production, Bioavailability, Health Potential, and Regulatory Issues, edited by John O. Onuh, M. Selvamuthukumaran, and Yashwant V. Pathak (ISBN: 978-0-3675-1177-7) Nutraceuticals for Prenatal, Maternal and Offspring's Nutritional Health, edited by Priyanka Bhatt, Maryam Sadat Miraghajani, Sarvadaman Pathak, and Yashwant V. Pathak (ISBN 978-1-1383-4582-9) Advances in Nutraceutical Applications in Cancer: Recent Research Trends and Clinical Applications, edited by Sheeba Varghese Gupta, and Yashwant V. Pathak (ISBN 978-1-1385-9391-6)

This volume brings together information on the available and newly emerging technologies related to using plant compounds that have a beneficial role in food production. It is divided into sections focusing on phytochemistry of cereals and legumes, phytochemistry of medicinal plants, and technological advances in phytochemical study. Topics include the role of anti-nutritional substances of legumes in human health and on the elimination of such through technological processing sorghum phytochemicals and their processing and use in the development of food products production of nutraceuticals and functional foods of pharmaceutical importance T. cordifolia in the development of its therapeutic use in the food, health, and pharmacology industries polyphenolic compounds of plants, including their biosynthesis process, their classification, function, and role as bioactive compounds

Comprehensively presents and discusses the wealth of information about pork products Includes specific details about the processing, quality of final products, and innovative aspects Presents innovative health-oriented approaches to elaborated traditional and commercial pork products Discusses healthier pork meat products that adhere to consumer trends and government recommendations

The dairy industry usually adopts conventional methods of processing various milk-based food products, which can destroy nutrients and minimize organoleptic qualities. An alternative approach for this is the non-conventional method of non-thermal processing techniques. Not only does this enhance the nutritional profile of the various processed products, but increases the consumer acceptability. There are some emerging non-thermal processing techniques such as pulsed light, cold plasma, high pressure processing, ultrasonic, UV pasteurization, or ozone treatments, which can be successfully employed in dairy processing industries to enhance product acceptability, safety, and quality aspects. Non-Thermal Processing Technologies for the Dairy Industry describes several emerging non-thermal processing techniques that can be specially employed for the dairy processing industry. The book narrates the benefits of using pulsed light, cold plasma, high pressure and ultrasonic during processing of various dairy products. Key Features: Addresses techniques used for extraction of functional food components from various dairy products by using super critical CO2 extraction technology. Explains application of ozone and cold plasma technology for treating dairy processing waste waters with efficient recycling aspects. Discusses the importance of using biopreservatives in shelf life extension of several dairy food products. Portrays scope and significant importance of adopting UV pasteurization in processing market milk along with safety and environmental impacts over processing This book solves the issue of waste generation in dairy industries and further advises recovery of such waste for efficient recycling process. In addition to being useful for dairy technologists, it is a great source for academic scholars and students looking to gain knowledge and excel in the non-thermal procesing area.

Imparts the techniques of commercial cultivation of mushrooms. Offers methods of macrofungal bioconversion of agrowastes. Provides a broad outline on macrofungal biofuel production. Furnishes outline on industrial potentials of white rot fungi by fermentation. Discusses bioactive metabolites and their application in remediating human lifestyle diseases. Converses nutritional benefits of macrofungi like vitamins, proteins, amino acids, minerals and polysaccharides. Communicates bioactive metabolites like perfumes, cosmeceuticals and pigments for food and textile industries. Denotes insight on industrial applications of wild mushrooms, mycelium-based products and wild mushrooms from forest to market. Proposes the prospects of melanin from higher fungi and their ex situ conservation, structural and functional attributes of melanin from termitophilic Agaricales.

Carnosine and anserine are two common histidine-derived dipeptides, also known as imidazole dipeptides, found in muscle and brain tissues of animals. Their dietary and nutritional significance lies in their antioxidant properties and a number of investigators are looking at other health benefits of the compounds. This volume surveys the current state of play in these and other areas of interest, including chemistry and biochemistry, immunology and cellular homeostasis. Chapters look in depth at some of the functions and effects in the body particularly with regard to exercise and fatigue, wound healing, cancer treatment and age-related diseases. Providing an up to date, interdisciplinary approach, this book is of great interest to researchers and professionals in chemistry, food science, nutrition, biochemistry, health sciences and sports sciences.

Cereals, pulses, roots, and tubers are major food sources worldwide and make a substantial contribution to the intake of carbohydrates, protein, and fiber, as well as vitamin E and B. The Handbook of Cereals, Pulses, Roots, and Tubers: Functionality, Health Benefits, and Applications provides information about commercial cereals, pulses, and their nutritional profile, as well as health benefits and their food and non-food applications. Split into four sections, this handbook covers all the recent research about the related crops and outlines matters needing further research in the field of agriculture sciences. Both qualitative and quantitative analysis of nutrients and bio-actives, and their beneficial effects on human health, are highlighted in this book. The conclusions drawn and future perspectives proposed in each chapter will also help researchers to take more focused approaches. FEATURES Covers the full spectrum of cereals, pulses, roots, and tubers grain production, processing, and their use for foods, feeds, fuels, and industrial materials, and other uses Contains the latest information from grain science professionals and food technologists alike Provides comprehensive knowledge on the nutritional and non-nutritional aspects of cereals, pulses, and tubers Discusses the latest development in modification of native starch Provides information in enhancing shelf life and its utilization in phytochemical rich product development The result of various well-versed researchers across the globe sharing their knowledge and experience, this handbook will be a valuable resource for students, researchers, and industrial practioners who wish to enhance their knowledge and insights on cereals, pulses, roots, and tubers.

Unit Operations in Winery, Brewery, and Distillery Design focuses on process design for wineries, breweries, and distilleries; and fills the need for a title that focuses on the challenges inherent to specifying and building alcoholic beverage production facilities. This text walks through the process flow of grapes to wine, grain to beer, and wine and beer to distilled spirits, with an emphasis on the underlying engineering principles, the equipment involved in these processes, and the selection and design of said equipment. Outlines the process flow of alcoholic beverage production Reviews process engineering fundamentals (mass & energy balances, fluid flow, materials receiving & preparation, heat exchange, fermentation, downstream processing, distillation, ageing, packaging, utilities, control systems, and plant layout) and their application to beverage plants Describes the idea of sanitary design and its application to plant operation and design Covers critical equipment parameters for purchasing, operating, and maintaining systems Shows how winery/brewery/distillery can influence product "style" and how "style" can dictate design Features examples of calculations derived from wineries designed by the authors, end of chapter problems, and integrative in-text problems that describe real-world issues and extend understanding Written for both engineers in the alcohol industry and non-engineers looking to understand facility design, this textbook is aimed at students, winemakers, brewers, distillers, and process engineers.

Following its predecessor, the second edition of Amino Acids: Biochemistry and Nutrition presents exhaustive coverage of amino acids in the nutrition, metabolism and health of humans and other animals. Substantially revised, expanded and updated to reflect scientific advances, this book introduces the basic principles of amino acid biochemistry and nutrition, while highlighting the current knowledge of the field and its future possibilities. The book begins with the basic chemical concepts of amnio acids, peptides and proteins, and their digestion and absorption. Subsequent chapters cover cell-, tissue-, and species-specific synthesis and catabolism of amino acids and related bioactive metabolites, and the use of isotopes to study amino acids metabolism in cells and the body. The book details protein turnover, physiological functions of amino acids, as well as both the regulation and inborn errors of amino acid metabolism. The book concludes with a presentation on human and animal dietary requirements of amino acids and evaluates dietary protein quality. Features: Encompasses a comprehensive coverage of basic to applied concepts in amino acid metabolism in humans and other animals. Highlights important roles of dietary amino acids and protein intake in growth, physical performance and health, including sarcopenia mitigation and immunity. Discusses concerns over the excess intakes of amino acids or protein in the development of diseases, including cardiovascular disorders, diabetes and cancers, as well as bone integrity Each chapter contains select references to provide comprehensive reviews and original experimental data on the topics discussed. Each chapter is backed by original experimental data on various topics discussed and contains select references to aid the reader further in research. Written by Distinguished Professor of Animal Nutrition, Guoyao Wu, Ph.D., this book is an authoritative reference for students and researchers in both biomedicine and agriculture.