Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.

Carefully delineating each step in the formation of the lung, Lung Growth and Development examines prenatal and postnatal lung development...the regulation of surfactant protein gene expression and models for the analysis of epithelial gene transcription and function...cellular differentiation and the role of mesenchymal cells...airway gland growth and differentiation...growth regulation in the tracheobronchial epithelium and mucociliary differentiation...embryonic precursors of the pulmonary nervous system and the development of lung innervation...Clara cells, airway smooth muscle development, cell interactions in vessel formation, and the surfactant system...respiratory distress syndrome, bronchopulmonary dysplasia, and compensatory lung growth...and more. Written by over 30 international experts, Lung Growth and Development is a practical guide for pulmonologists and pulmonary disease specialists, physiologists, molecular and cellular biologists, pathologists, neonatalogists and pediatricians, anatomists, pathologists, and graduate-level and medical school students in these disciplines.

This book focuses on cell culture-produced viral vaccines to meet the needs of the rapidly expanding research and development in academia and industry in the field. This book introduces the basic principles of vaccination and the manufacturing of viral vaccines. Bioprocessing of Viral Vaccines, will provide an overview of the advanced strategies needed to respond to the challenges of new and established viral infection diseases. The first few chapters cover the basics of virology and immunology as essential concepts to understand the function and design of viral vaccines. The core of the content is dedicated to process development, including upstream processing and cell culture of viral vaccines, downstream processing, and extensive analytical technologies specific to viral vaccines. Advanced process analytical technologies (PAT) and Quality by Design (QbD) concepts are also introduced in the context of vaccine manufacturing. The case studies included cover inactivated, attenuated vaccines exemplified by influenza vaccines, sub-unit vaccines exemplified by Virus Like Particles (VLPs: HPV vaccines) and sub-unit vaccines (Flublock), vectored vaccines: adenoviruses and Vesicular stomatitis Virus (VSV) vectored vaccines, genomic vaccines (DNA and mRNA) vaccines as developed for COVID-19 response in particular and a review of COVID-19 vaccines approved or in advanced clinical trials. This book is aimed at graduate engineers and professionals in the fields of vaccinology, bioprocessing, and biomanufacturing of viral vaccines.

This clear, concise Complete Revision & Practice book from CGP is a perfect way to prepare for the Edexcel A A-Level Biology exams - it covers every topic from both years of the course. It's fully up-to-date for the new exam specifications for 2015 and beyond, with straightforward explanations, helpful examples and full-colour diagrams throughout. Practice questions and exam-style questions (with answers) are included for every topic, and the book is rounded off with a section of in-depth advice on Practical Skills. And finally, a free Online Edition of the whole book is included - just use the code printed inside the book to access it on your PC, Mac or tablet!

Real-world evidence (RWE) has been at the forefront of pharmaceutical innovations. It plays an important role in transforming drug development from a process aimed at meeting regulatory expectations to an operating model that leverages data from disparate sources to aid business, regulatory, and healthcare decision making. Despite its many benefits, there is no single book systematically covering the latest development in the field. Written specifically for pharmaceutical practitioners, Real-World Evidence in Drug Development and Evaluation, presents a wide range of RWE applications throughout the lifecycle of drug product development. With contributions from experienced researchers in the pharmaceutical industry, the book discusses at length RWE opportunities, challenges, and solutions. Features Provides the first book and a single source of information on RWE in drug development Covers a broad array of topics on outcomes- and value-based RWE assessments Demonstrates proper Bayesian application and causal inference for real-world data (RWD) Presents real-world use cases to illustrate the use of advanced analytics and statistical methods to generate insights Offers a balanced discussion of practical RWE issues at hand and technical solutions suitable for practitioners with limited data science expertise

Cyber Security Threats and Challenges Facing Human Life provides a comprehensive view of the issues, threats, and challenges that are faced in the cyber security domain. This book offers detailed analysis of effective countermeasures and mitigations. The financial sector, healthcare, digital manufacturing, and social media are some of the important areas in which cyber-attacks are frequent and cause great harm. Hence, special emphasis is given to the study and analysis of cyber security challenges and countermeasures in those four important areas. KEY FEATURES * Discusses the prominence of cyber security in human life * Discusses the significance of cyber security in the post-COVID-19 world * Emphasizes the issues, challenges, and applications of cyber security mitigation methods in business and different sectors * Provides comphrension of the impact of cyber security threats and challenges in digital manufacturing and the internet of things environment * Offers understanding of the impact of big data breaches and future trends in data security This book is primarily aimed at undergraduate students, graduate students, researchers, academicians, and professionals who are interested in exploring their research and knowledge in cyber security domain.

This book brings together scientists from all over the world who have defined and developed the field of Coordination Dynamics. Grounded in the concepts of self-organization and the tools of nonlinear dynamics, appropriately extended to handle informational aspects of living things, Coordination Dynamics aims to understand the coordinated functioning of a variety of different systems at multiple levels of description. The book addresses the themes of Coordination Dynamics and Dynamic Patterns in the context of the following topics: Coordination of Brain and Behavior, Perception-Action Coupling, Control, Posture, Learning, Intention, Attention, and Cognition.

Only a fifth of adults in the United States do enough physical activity to meet the guidelines set by Centers for Disease Control. The health benefits of regular physical activity are beyond dispute, yet less than 40% of physicians routinely counsel their patients on the importance of physical activity. Increasing Physical Activity: A Practical Guide equips healthcare practitioners to include physical activity counseling in the daily practice of medicine. Written by lifestyle medicine pioneer and cardiologist, Dr James Rippe, this book proves inactivity is a stronger risk factor than other lifestyle factors for cardiovascular disease, diabetes, and many other diseases. It provides evidence-based information on the role of physical activity in preventing and treating chronic conditions and includes practical strategies for healthcare practitioners to prescribe this powerful method to enhance both short and long-term health and quality of life. Features: Specific chapters explain the role of physical activity in reduction of risk of heart disease, diabetes, cancer, osteoarthritis, dementia and many other chronic conditions. Chapters begin with bulleted, key points and conclude with a list of clinical applications. Strategies are provided to encourage previously sedentary individuals to adopt regular physical activity. Physical activity is placed in the context of other lifestyle medicine concepts including maintenance of a healthy body weight, following sound nutritional practices, stress reduction and other practices which impact on health and quality of life. Written for healthcare practitioners at all levels, this is a user-friendly, evidence-based manual for healthcare practitioners looking to incorporate more physical activity counseling into either general medicine or subspecialty practices.

Women are contributing to disciplines once the sole domain of men. Field biology has been no different. The history of women field biologists, embedded in a history largely made and recorded by men, has never been written. Compilations of biographies have been assembled, but the narrative-their story-has never been told. In part, this is because many expressed their passion for nature as writers, artists, collectors, and educators during eras when women were excluded from the male-centric world of natural history and science. The history of women field biologists is intertwined with men's changing views of female intellect and with increasing educational opportunities available to women. Given the preponderance of today's professional female ecologists, animal behaviorists, systematists, conservation biologists, wildlife biologists, restoration ecologists, and natural historians, it is time to tell this story-the challenges and hardships they faced and still face, and the prominent role they have played and increasingly play in understanding our natural world. For a broader perspective, we profile selected European women field biologists, but our primary focus is the journey of women field biologists in North America. Each woman highlighted here followed a unique path. For some, personal wealth facilitated their work; some worked alongside their husbands. Many served as invisible assistants to men, receiving little or no recognition. Others were mavericks who carried out pioneering studies and whose published works are still read and valued today. All served as inspiration and proved to the women who would follow that women are as capable as men at studying nature in nature. Their legacy lives on today. The 75 female field biologists interviewed for this book are further testament that women have the intellect, stamina, and passion for fieldwork.

This pioneering handbook serves as an essential tool for any biobanking entity to create, implement and continuously improve their Quality Management System (QMS). Written in a concise and highly readable manner all biobanking related QMS aspects, ranging from legal aspects to safety matters, are addressed according to the best knowledge in compliance with the dedicated Biobanks ISO standards. Following a practical approach by making use of FAQ and common practice sections this book guides the readers through this complex field in an easy-to understand way.The guidelines are convergent not only with ISO 20387:2018 Biotechnology - Biobanking - General requirements for biobanking but also with ISO 9001:2015, ISO 19011:2018, ISO 27000:2014, and ISO 27002:2013. Furthermore, they are compatible, among others with the recommendations of the Organization for Economic Cooperation and Development (OECD), IARC, and ISBER Best Practices. Aimed at both biobank employees and other stakeholders (e.g. public bodies, political bodies, hospitals, pharmaceutical industry, funders) at any level of experience the book serves as valuable source for self-education and teaching. The manual complies to the principles of responsibility, openness, and transparency and can be used by any biobanking unit regardless of the biological material the biobank operates with and independent of their associated biobank network. On behalf of a group of specialists and experts in the area of biobanking, regarding Quality Management Systems (QMS), Ethical, Legal and Societal Issues (ELSI) and IT solutions, the authors present with this book a significant achievement based on activities within the project, European Research Infrastructure BBMRI-ERIC "Quality Standards for Polish Biobanks" Handbook (QSPB).

Beginning his work on the monograph to be published in English, this author tried to present more or less general notions of the possibilities of mathematics in the new and rapidly developing science of infectious immunology, describing the processes of an organism's defence against antigen invasions. The results presented in this monograph are based on the construc tion and application of closed models of immune response to infections which makes it possible to approach problems of optimizing the treat ment of chronic and hypertoxic forms of diseases. The author, being a mathematician, had creative long-Iasting con tacts with immunologists, geneticist, biologists, and clinicians. As far back as 1976 it resulted in the organization of a special seminar in the Computing Center of Siberian Branch of the USSR Academy of Sci ences on mathematical models in immunology. The seminar attracted the attention of a wide circle of leading specialists in various fields of science. All these made it possible to approach, from a more or less united stand point, the construction of models of immune response, the mathematical description of the models, and interpretation of results."

Biology is in the midst of a era yielding many significant discoveries and promising many more. Unique to this era is the exponential growth in the size of information-packed databases. Inspired by a pressing need to analyze that data, Introduction to Computational Biology explores a new area of expertise that emerged from this fertile field- the combination of biological and information sciences.

This introduction describes the mathematical structure of biological data, especially from sequences and chromosomes. After a brief survey of molecular biology, it studies restriction maps of DNA, rough landmark maps of the underlying sequences, and clones and clone maps. It examines problems associated with reading DNA sequences and comparing sequences to finding common patterns. The author then considers that statistics of pattern counts in sequences, RNA secondary structure, and the inference of evolutionary history of related sequences.

Introduction to Computational Biology exposes the reader to the fascinating structure of biological data and explains how to treat related combinatorial and statistical problems. Written to describe mathematical formulation and development, this book helps set the stage for even more, truly interdisciplinary work in biology.

*Provides an overview of statistical and analytic methodologies in real-world evidence to generate insights on healthcare, with a special focus on the pharmaceutical industry *Examines timely topics of high relevance to industry such as bioethical considerations, regulatory standards and compliance requirements *Highlights emerging and current trends, and provides guidelines for best practices *Illustrates methods through examples and use-case studies to demonstrate impact *Provides guidance on software choices and digital applications for successful analytics.

The Routledge Handbook of Feminist Philosophy of Science is a comprehensive resource for feminist thinking about and in the sciences. Its 33 chapters were written exclusively for this Handbook by a group of leading international philosophers as well as scholars in gender studies, women's studies, psychology, economics, and political science. The chapters of the Handbook are organized into four main parts: I. Hidden Figures and Historical Critique II. Theoretical Frameworks III. Key Concepts and Issues IV. Feminist Philosophy of Science in Practice. The chapters in this extensive, fourth part examine the relevance of feminist philosophical thought for a range of scientific and professional disciplines, including biology and biomedical sciences; psychology, cognitive science, and neuroscience; the social sciences; physics; and public policy. The Handbook gives a snapshot of the current state of feminist philosophy of science, allowing students and other newcomers to get up to speed quickly in the subfield and providing a handy reference for many different kinds of researchers.

-- Finishing and publishing a PhD is daunting as, for most students, it will be their first experience working within the academic system. This guide offers a helping hand during and when making decisions about how to move on with their career, specifically in the biological sciences. -- Examples are tailored to biological science, offering a unique reference for PhD students in these disciplines. -- The author has authored more than 200 peer reviewed scientific papers and book chapters, and five books. He has been the Editor-in-Chief of an ISI journal for 9 years, and has graduated more than 20 postgraduate students. His blog on writing and publishing in biological sciences is read by thousands globally. -- Most of the 25,000 universities in the world have postgraduates in biological sciences, and emerging economies, such as India and China, will have special interest in this book as their academic systems still fall outside of the academic mainstream. -- The book has many short, easy to read, chapters which are interconnected to provide a comprehensive treatment of each subject, and it explore the 'hot' topics in academic publishing, from Open Access to new blockchain models, as well as academic bullying.

Elastic and inelastic scattering in transmission electron microscopy (TEM) are important research subjects. For a long time, I have wished to systematically summarize various dynamic theories associated with quantitative electron micros copy and their applications in simulations of electron diffraction patterns and images. This wish now becomes reality. The aim of this book is to explore the physics in electron diffraction and imaging and related applications for materials characterizations. Particular emphasis is placed on diffraction and imaging of inelastically scattered electrons, which, I believe, have not been discussed exten sively in existing books. This book assumes that readers have some preknowledge of electron microscopy, electron diffraction, and quantum mechanics. I anticipate that this book will be a guide to approaching phenomena observed in electron microscopy from the prospects of diffraction physics. The SI units are employed throughout the book except for angstrom (A), which is used occasionally for convenience. To reduce the number of symbols used, the Fourier transform of a real-space function P'(r), for example, is denoted by the same symbol P'(u) in reciprocal space except that r is replaced by u. Upper and lower limits of an integral in the book are (-co, co) unless otherwise specified. The (-co, co) integral limits are usually omitted in a mathematical expression for simplification. I very much appreciate opportunity of working with Drs. J. M. Cowley and J. C. H. Spence (Arizona State University), J."

Offering a rich diversity of models, Bayesian phylogenetics allows evolutionary biologists, systematists, ecologists, and epidemiologists to obtain answers to very detailed phylogenetic questions. Suitable for graduate-level researchers in statistics and biology, Bayesian Phylogenetics: Methods, Algorithms, and Applications presents a snapshot of current trends in Bayesian phylogenetic research. Encouraging interdisciplinary research, this book introduces state-of-the-art phylogenetics to the Bayesian statistical community and, likewise, presents state-of-the-art Bayesian statistics to the phylogenetics community. The book emphasizes model selection, reflecting recent interest in accurately estimating marginal likelihoods. It also discusses new approaches to improve mixing in Bayesian phylogenetic analyses in which the tree topology varies. In addition, the book covers divergence time estimation, biologically realistic models, and the burgeoning interface between phylogenetics and population genetics.

This book is a cohesive guide to the available methods that can be used in population viability analysis. It is therefore extremely valuable to both the practitioner of conservation biology and the theoretical population biologist.