The Color Atlas of "Xenopus laevis" Histology provides the first central source on the microscopic anatomy of cells, tissues, and major organs of the adult South African clawed frog, Xenopus laevis. For many years, X. laevis has been a highly popular experimental animal model in many areas of research. The recent development of transgenic Xenopus technology offers the promise that this animal model will be utilized more than ever before. The purpose of this book is to provide the active researcher with a central source of high quality light microscopic color images of the tissues of X. laevis, to aid in the identification of the cells and tissues of interest.

Major features of this atlas include:

*277 large, colorful, high resolution light microscopic images of cells, structures, tissues and organs;
*Clear, detailed labeling of important structures in all images;
*Concise, easy-to-read descriptions of histological organization and functions;
*Colorful three-dimensional illustrations that simplify complex structure;
*Detailed presentation of cranial structures and organization;
*Easy-to-use cross-referenced index;
*Bundled CD/ROM containing all images for easy downloading.

There is general agreement that increased environmental pollution poses a potential health hazard to humans and that effective control of such genetic injury requires monitoring the exposed individuals for genetic damage and identifying chemicals that may cause mutation or cancer. Tests available for identifying mutagens or carcinogens range from relatively simple, rapid assays in prokaryotes and test systems utilizing mammalian cells in tissue culture to highly elaborate tests in intact animals. No single test can provide data for an unequivocal assessment of the mutagenicity of a given chemical and the risk it might pose to human health. A tier approach, therefore, was suggested for mutagenicity testing in which the suspected agents would be initially evaluated with simple, inexpensive tests that would give qualitative results. Chemicals found to be positive in the first-tier testing would then be evaluated with more complex tests, including those based on mammalian cells in culture. Testing in the final tier requires whole-animal studies, and is expensive and time-consum ing, and even the results from these studies need to be extrapolated for human risk assessment. The mutation systems based on whole animals require scoring large num bers of animals, and therefore are not practical for the routine testing of muta gens. As an alternative to monitoring the pedigree, cells from exposed individ uals may be considered for screening for point mutations through the use of an appropriate marker protein."

Toph's in Chemical Mutagenesis is a new series dedicated to studies in the areas of environmental chemical mutagenesis and genetic toxicology. In this series we will explore some of many topics that are emerging in these rapidly developing fields. The purpose of the present volume is to attempt to organize and compare the genotoxic properties of the N-nitroso compounds. This is a particularly interesting class of compounds because of the problems encountered with the Salmonella assay of Ames in generating both false positive and false negative results. The battery approach using a number of assay systems seems more appropriate to evaluate chemicals in this class. Topics to be discussed in other volumes in this series include single-cell mutation monitoring systems, the detection of genetic damage in mammalian germ cells, the mutagenicity of pesticides, problems in monitoring human populations in genetic toxicology, and a glossary of terms in genetic toxicology. All of these books are in various stages of development and should appear within the next few years. Frederick J. de Serres Series Editor vii Preface During the past ten years there has been an explosive development in the number of short-term tests to predict the biological risks, especially risks of cancer, in exposure to xenobiotic chemicals. The number of published articles in this area has reached many thousands a year and there are several new journals devoted almost entirely to the presentation of the results obtained in these tests.

Poisons are topics of multidisciplinary concern. The clinician and the pathologist are sensitive to instances of human poisoning. The laboratory researcher, whether pharmacologist, physiologist, or biochemist, is oriented toward molecular modes of poison action. Both clinician and researcher are eager to learn of poisons that can be used as therapeutic agents or methodological tools. This volume is an attempt to underscore the multidisciplinary charac ter of neuropoisons. Six poisons of animal origin which are receiving considerable clinical and research attention are discussed. Each poison is presented first as a clinical entity, then as a topic of investigative research, and finally as an agent useful to the study of nerve function. Because no single volume on neuropoisons can be exhaustive, an attempt at balance is offered as compensation. Two snake venoms, two marine poisons, and two bacterial toxins are presented in detail. In the sequel to this volume, attention will be focused on representative neuro poisons of plant origin."

This publication contains the proceedings of a seminar held in Brussels on November 8-9, 1988. The title of the seminar was "Reducing the costs of disease by improving resistance through genetics." The seminar was held as an activity of the Community Programme for the Coordination of Agricultural Research, 1984-1988. Costs of disease depend on losses caused by morbidity, mortality and production decreases and on the costs of preventive measures including vaccination and medication. Production losses often contribute a major portion to the total costs. To reduce costs of disease preventive measures like vaccination, preventive medication and hygienic procedures are applied. Genetic resistance is an attractive preventive measure because of its consistent nature in the next generations, because it precludes veterinary services and because there are no side-effects. Constraints are the long term investment, relatively slow progress per generation (in combination with production traits) and the considerable lack of knowledge about inheritance of resistance mechanisms in farm animals.

This book provides an extensive review of research into Campylobacter, Helicobacter and Arcobacter species found in poultry. It includes the epidemiology, diagnosis, immune response and disease control of these organisms in commercial poultry production. Antimicrobial resistance, and the incidence and human disease potential of these bacteria is also discussed. A global perspective is presented by experts from four continents - South America, North America, Europe and Africa. This reference work will be of value to the poultry industry, research laboratories, public health workers and students. An extensive overview of the relevant literature is provided by the reference lists at the end of each chapter.

This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 2: Vaccines for Veterinary Diseases includes vaccines for farm animals and fishes, vaccine vectors and production, vaccine delivery systems, vaccine bioinformatics, vaccine regulation and intellectual property. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 2: Vaccines for Veterinary Diseases aims to ensure successful results in the further study of this vital field.

This volume reviews the current understanding of the taxonomy, disease syndromes, genetics, biology, and pathogenic factors of Histophilus somni, as well as the host immune response to this pathogen. H. somni is one of the most important bacterial pathogens in cattle and other ruminants, and its virulence factors are highly conserved with Haemophilus influenzae and other members of the Pasteurellaceae. H. somni has been recognized as a major cause of thrombotic meningoencephalitis, respiratory disease syndromes, myocarditis, reproductive disease syndromes, polyarthritis, mastitis, ocular disease, and septicemia. The only known habitats of H. somni are the mucosal surfaces of ruminants, making this bacterium an opportunistic pathogen. Although it is capable of causing inflammation at systemic sites and is toxic to epithelial and phagocytic cells, the bacterium's wide array of virulence factors act primarily as a defense against, or to escape recognition from, host innate and adaptive immunity.

This book is based on the proceedings of a seminar on acute viral in fections of poultry, which was held in Brussels on 13-14 June 1985. The aim of the seminar, which was sponsored by the CEC, was to gather infor mation on those infections of immediate or increasing importance. It is hoped that dissemination of this information will make it easier to har monize diagnostic and control measures throughout the member states of the EEC. Several points emerge from recent outbreaks of highly pathogenic avian influenza. In view of the apparent change in pathogenicity of the virus involved in the outbreak in the USA, infections of domestic poultry, particularly with H5 and H7 sub-types, should be regarded as a potential threat, even if the viruses involved are of low pathogenicity. The reasons for the amount of lateral spread which occurred in the USA outbreak are still not clear, and it can not be assumed that future outbreaks of highly pathogenic avian influenza will tend to be self-limiting, as in the past. The importance of denying access of wild birds, particularly water fowl, to domestic poultry needs to be re-emphasised. Lastly, there appears to be increasing support for a combination of slaughter and ring vaccination to control future outbreaks of spreading avian influenza."

The transport of farm livestock was the subject of the seminar held from 7 - 8 July 1981 at the Commission of the European Communities (CECl, Directorate General for Agriculture, Brussels as part of the work of the Division Coordinating Agricultural Research. The aims of the seminar were to examine the knowledge available on how the physiology and behaviour of animals may change during transport; to consider the significance of these changes in relation to welfare and economics and to assess those actions which as experimental projects or observational studies might be proposed to fill the most important gaps in our knowledge of the welfare of farm animals during transport. A number of conclusions can be drawn from the proceedings: 1. Much knowledge is available from both scientific observations and practical experience which could be used to improve the transport of livestock. Methods of loading, the construction of vehicles, ships, crates and aircraft could benefit from the application of existing knowledge. It is less clear whether it is best to concentrate on disseminating existing knowledge by education and advice or to contemplate more regulations. 2. Losses by down-grading at slaughter can largely be attributed to the ways in which animals are transported and handled.

This publication is the Proceedings of a workshop held at the Royal Veterinary and Agricultural University in Copenhagen, Denmark on 4th - 6th February, 1980, sponsored by the Commission of the EUropean Communities (CEO) as a part of the programme of coordination of agricultural research in the field of animal pathology. The CEO wishes to thank those who took responsibility for the organisation of the workshop, those who presented the papers, and all participants. VII CONTENTS SESSION I METHODOLOGY I MONITORING PASTURE INFECTIVITY AND PASTURE CONTAMINATION WITH INFECTIVE STAGES OF Dictyocaulus viviparus 3 R. J. Jcentsrgensen THE CORRECT HANDLING OF FAECAL SAMPLES USED FOR EXAMINATION OF Dictyocaulus viviparus LARVAE 11 H. J. W. M. Cremers SOME EFFECTS OF STORAGE ON THE RECOVERY OF Dictyocaulus viviparus LARVAE FROM FAECES 17 M. T. Fox EXPERIENCES WITH OUR TECHNIQUES FOR THE RECOVERY OF NEMATODE LARVAE FROM HERBAGE H. -J. Burger 25 A TECHNIQUE FOR THE RECOVERY OF INFECTIVE TRICHOSTRONGYLE LARVAE FROM SOIL 31 K. Bairden, J. L. Duncan and J. Armour SESSION I METHODOLOGY II A MODIFIED AND SIMPLE MCMASTER TECHNIQUE 45 Sv. Aa. Henriksen COMPARISON OF TECHNIQUES FOR ASSESSMENT OF THE CONTAMINATION OF PASTURE HERBAGE WITH INFECTIVE NEMATODE LARVAE 51 J. -P. Raynaud and L. Gruner RECOVERY OF Ostertagia FROM THE BOVINE ABOMASAL MUCOSA BY IMMERSION IN WARM NORMAL SALINE 69 N. E. Downey CONTROLLED/CRITICAL TESTS IN THE EVALUATION OF ANTHELMINTIC ACTIVITY 75 D. D{

The welfare of domestic poultry. particularly those kept under intensive housing conditions is a subject ~n which many. often divergent and conflicting views. are held. This divergence, may be the result either of insufficient knowledge of the facts of particular poultry husbandry systems or a differing interpretation of those facts. With regard to poultry and the laying hen in particular. there is a need to gather together a basic knowledge of avian behaviour in all the circumstances and systems of husbandry under which birds are presently being kept. That knowledge should lead to the development of interpretative and hopefully predictive theories which in turn will allow us to meet the recommendations of Article 3 of the Council of Europe Convention on the Protection of Animals Kept for Farming Purposes: 'that animals shall be housed, provided with food. water and care in a manner which - having regard to their species and to their degree of development. adaptation and domestication - is appropriate to their physiological and ethological needs in accordance with established experience and scientific knowledge'. In furtherance of those objectives a small group of specialists in poultry physiology and ethology. both from within and outside the European Community met by invitation of the Commission in Luxembourg between 11 and 13 March 1980. Their objective was to discuss what is already known. and can be agreed on. of the normal physiology and ethological range of the laying hen.

The success of a scientific workshop depends on a delicate blend of many types of ingredients. Most important is to select a provocative topic which is at the forefront of a current investigative study. Coupled together with a relatively small but distinguished group of active research scientists known for their continued record of contributing significant findings, one has the firm foundation for an exciting and rewarding investment of time and effort. This was the setting for the first workshop organized by the European Association for Veterinary Pharmacology and Toxicology. Ruminants have been domesticated for many centuries and have served mankind as a source of dairy products, meat, wool and power. The ruminant stomach has long been - and still is - a major concern for physiologists, pathologists, clinicians and pharmacologists. This workshop was organized and convened in an attempt to strengthen the basic science of the ruminant stomach, as it applies to an economically important group of mammals. To achieve this, various topics were covered by specialists which ensured presentation of new data, followed by discussions. In this book, reviews are presented on the different topics: motility (control and regulation, neurotransmitters and endogenous substances involved); flow of digesta (comparative aspects, role of content and metabolites); food intake; rumen metabolism (chemical manipulation, metabolism of xenobiotics and drugs); pharmacology of forestomach motility and, the reticulo-rumen as a pharmacokinetic compartment.

Animal production systems have changed dramatically over the last two decades. Knowledge of energy metabolism and environmental physio logy has increased as appears from many textbooks on these disciplines. The contents of the symposia on energy metabolism of farm animals show this and they have initially focussed on feed evaluation and later on com parative aspects of energy metabolism. They show part of the progress being made. Application of knowledge of energy metabolism for animals has a long history since Lavoisier. In addition to this, studies about the environ mental requirements of animals have shown that we are still far from ac curate assessment of these requirements in terms of nutrients and ener gy. I n model studies on energy metabolism researchers have recognized the interaction between the environment and the energy requirements of animals. Estimation of energy requirements has been done in physiolo gical, physical and behavioural studies. The impact of conditions as en countered by animals in various production systems has been approached from different viewpoints related to these different disciplines. In addi tion, various kinds of infections (bacterial, parasitic: subclinical, clini cal) have been evaluated only recently with regard to their effect on pro tein and/or energy metabolism and thus on production. People working in the field of feed evaluation have defined how che mical and physical properties of nutrition infiuence energy to be derived for maintenance and production.

This second volume of papers dealing with scientific and ethical aspects of animal welfare covers a variety of topics and areas of inves tigation. It will be of particular interest to those readers seeking more insight into such subjects as farm animal welfare and humane husbandry systems; animal experimentation, especially in the field of psychology; and pain in animals, notably its recognition and alleviation. Several of our selections deal with very specific subjects that are germane to animal welfare: the use of T-61 for euthanizing cats and dogs, a new humane method of stunning for livestock and poultry, an innovative alternative to killing animals for rabies diagnosis, alterna tives to aversive procedures in teaching experimental psychology, and the need for improved theoretical modeling in animal experimentation and research design. Following the precedent set in the first volume of Advances in Animal Welfare Science, we have included several papers dealing with people's attitudes toward animals. These papers range from a consider ation of cultural influences and veterinary ethics to an examination of anthropomorphism, to a discussion of the linkage between the environ mental politics and perceptions of the Green Movement and animal welfare and rights. We wish to express our gratitude to the Manuscript Review Commit tee for the excellent work they have done and to the twenty contributors to this volume which we believe will do much to advance the science of animal welfare, and the well-being of animals under man's dominion."

This publication contains the proceedings of a seminar held in Germany (Fed. Rep.) on May 4 -6, 1977, under the auspices of the Commission of the European Communities, as part of the EEC programme of co ordination of research on beef production. The programme was drawn up by a combined scientific working group on Genetics and Selection and on Nutrition and Management on behalf of the Beef Production Committee. The working group consisted of Professor Dr. Neimann S rensen (Denmark, Chairman), Dr. Brolund Larsen (Denmark), Mr. Boccard (France), Dr. H. de Boer (Netherlands), Priv.-Doz. Dr. B. Hoffmann (Germany, Fed. Rep.), Professor Dr. H.J. Langholz (Germany, Fed. Rep.), Dr. J.W.B. King (UK), Mr. R. Jarrige (France), Mr. B. Vissac (France), Professor Dr. A. Romita (Italy), Professor Dr. E.P. Cunningham (Ireland), Mr. P. L'Hermite (CEC) and Dr. J.C. Tayler (Scientific Adviser to CEC). The subject chosen for this seminar was drawn from the list of priorities in research objectives drawn up in 1973 by members of a committee (now the Standing Committee on Agricultural Research, ( PRA). One of the functions of this series of seminars was to summarise and update the information available on the selected subjects and to discuss future needs for research, so as to assist the Commission in evaluating the probable impact of research on agri cultural production within the Community."

This book, the first in an annual series, written by academicians scientists, philosophers and others-is not intended exclusively for an imal welfarists and conservationists. Since it is written* by scholars, it will appeal to a wide range of academic and professional readers who are involved with animals for scientific, economic, altruistic, and other reasons. While this first volume cannot cover the entire spectrum of animal welfare science-related topics, it does, in its diversity of con tributions, demonstrate the multi-faceted and interdisciplinary nature of the subject of this new series. Indeed, animals are as much an integral part of society as we are dependent upon them. The many interfaces between us and the billions of animals under our dominion (as well as the environment upon which the welfare of human and non-human animals alike is ultimately de pendent) have their separate features: trapping and wildlife manage ment; laboratory animal research; whaling and fishing; veterinary practice; agriculture and farm animal husbandry; horse racing and the ownership of animal companions; the propagation of captive wildlife and their preservation in the wild; the use of animals as companions and for the purposes of vicarious entertainment."

In this cutting-edge text, Thomas Catanzaro brings his considerable experience and training to bear on management in the veterinary profession. He discusses changes emerging in the twenty-first century veterinary medical practice and the alterations they demand in management style and practice organization. Besides the techniques and concepts for developing new programs and procedures, Dr. Catanzaro provides real-life applications for immediate use by veterinary practitioners around the world. "Veterinary Management in Transition "specifically addresses the following areas:

* Are you and your practice ready for change?;

* What is governance?;

* Why is it needed?;

* How does it work?;

* In group practices, how can the leadership role be shared?;

* What issues do you need to consider when hiring a group administrator, and how do you train him or her?;

* How can the transition from traditional, single-doctor forms of management to new, more effective forms be made?

A self-assessment at the end of each chapter helps readers understand their own strengths and weaknesses. Generous appendixes offer stand-alone reviews of the concepts presented in the text as well as model administrative action plans for staff development and change management.

The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized - and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.

The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized - and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.

The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized - and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.

The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized - and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.

The Springer Handbook of Enzymes provides concise data on 4,457 enzymes - and here is the second, updated edition. The enzymes are sufficiently well characterized for application in analytical, synthetic and biotechnology processes as well as in food industry. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus synonym index.