Toph's in Chemical Mutagenesis is a new series dedicated to studies in the areas of environmental chemical mutagenesis and genetic toxicology. In this series we will explore some of many topics that are emerging in these rapidly developing fields. The purpose of the present volume is to attempt to organize and compare the genotoxic properties of the N-nitroso compounds. This is a particularly interesting class of compounds because of the problems encountered with the Salmonella assay of Ames in generating both false positive and false negative results. The battery approach using a number of assay systems seems more appropriate to evaluate chemicals in this class. Topics to be discussed in other volumes in this series include single-cell mutation monitoring systems, the detection of genetic damage in mammalian germ cells, the mutagenicity of pesticides, problems in monitoring human populations in genetic toxicology, and a glossary of terms in genetic toxicology. All of these books are in various stages of development and should appear within the next few years. Frederick J. de Serres Series Editor vii Preface During the past ten years there has been an explosive development in the number of short-term tests to predict the biological risks, especially risks of cancer, in exposure to xenobiotic chemicals. The number of published articles in this area has reached many thousands a year and there are several new journals devoted almost entirely to the presentation of the results obtained in these tests.

Approximately five years have elapsed since the Conference on "Tick-borne Diseases and their Vectors" (Wilde, 1978, University of Edinburgh) was held at the Centre for Tropical Veterinary Medicine in Edinburgh. Theileriosis was one of the main topics at that Conference and some 20 scientific presentations were given. Also in the same year a Workshop on "Theileriosis" was held at the Kenyatta Conference Centre in Nairobi (Henson & Campbell, 1977, IDRC, Ottawa). Both of these meetings provided a valuable up dating of theilerial diseases, and the Proceedings have been a constant source of reference for scientists in the ensuing years. The meetings played a significant role in setting the scene for a number of important advances which have been made since then. In February of this year, attention was focused on these advances when nearly 200 scientists from over 30 countries were assembled at the International Laboratory for Research on Animal Diseases in Nairobi for the international conference on "Advances in the Control of Theileriosis." The interest and concern shown in this subject has now grown to the extent that more than 70 scientific presentations were given over the course of a very busy week. An important facet of the Conference was the attention given to the control of Theileriosis, since this must be the ultimate aim of all those involved with the disease. Control will be difficult."

This second volume of papers dealing with scientific and ethical aspects of animal welfare covers a variety of topics and areas of inves tigation. It will be of particular interest to those readers seeking more insight into such subjects as farm animal welfare and humane husbandry systems; animal experimentation, especially in the field of psychology; and pain in animals, notably its recognition and alleviation. Several of our selections deal with very specific subjects that are germane to animal welfare: the use of T-61 for euthanizing cats and dogs, a new humane method of stunning for livestock and poultry, an innovative alternative to killing animals for rabies diagnosis, alterna tives to aversive procedures in teaching experimental psychology, and the need for improved theoretical modeling in animal experimentation and research design. Following the precedent set in the first volume of Advances in Animal Welfare Science, we have included several papers dealing with people's attitudes toward animals. These papers range from a consider ation of cultural influences and veterinary ethics to an examination of anthropomorphism, to a discussion of the linkage between the environ mental politics and perceptions of the Green Movement and animal welfare and rights. We wish to express our gratitude to the Manuscript Review Commit tee for the excellent work they have done and to the twenty contributors to this volume which we believe will do much to advance the science of animal welfare, and the well-being of animals under man's dominion."

This book is based on the proceedings of a seminar on acute viral in fections of poultry, which was held in Brussels on 13-14 June 1985. The aim of the seminar, which was sponsored by the CEC, was to gather infor mation on those infections of immediate or increasing importance. It is hoped that dissemination of this information will make it easier to har monize diagnostic and control measures throughout the member states of the EEC. Several points emerge from recent outbreaks of highly pathogenic avian influenza. In view of the apparent change in pathogenicity of the virus involved in the outbreak in the USA, infections of domestic poultry, particularly with H5 and H7 sub-types, should be regarded as a potential threat, even if the viruses involved are of low pathogenicity. The reasons for the amount of lateral spread which occurred in the USA outbreak are still not clear, and it can not be assumed that future outbreaks of highly pathogenic avian influenza will tend to be self-limiting, as in the past. The importance of denying access of wild birds, particularly water fowl, to domestic poultry needs to be re-emphasised. Lastly, there appears to be increasing support for a combination of slaughter and ring vaccination to control future outbreaks of spreading avian influenza."

This seminar referred to latent herpes virus infections in veterinary medicine, with emphasis on the domestic animals. The phenomenon of latency is of particular importance in veterinary medicine because it can jeopardize the successful control and eradication of diseases such as Aujeszky's disease and infectious bovine rhinotracheitis, diseases which are often the cause of great economic losses. For this reason, the Commission of the European Communities (CEC) realized the importance of summarizing the present state of knowledge on latent herpes virus infections in veterinary medicine. A seminar was therefore organized by the Federal Research Institute for Animal Virus Diseases in Ttibingen, Federal Republic of Germany, from September 21 to 23, 1982, as a part of the 'Animal Pathology Programme' of the CEC. The seminar was attended by 50 participants not only from the countries of the CEC, and 38 papers were selected for presentation. In veterinary medicine, more intensive investigations on the latency of animal herpes viruses have only relatively recently been initiated. In contrast, great efforts have been made for many years to elucidate latency in human and primate herpes viruses, and consequently the most considerable advances have been made in this field. Some of the most experienced scientists working on both systems were therefore invited. As a result, this seminar was the first occasion on which scientists from different countries and faculties investigating herpes virus latency had the opportunity to present and exchange their latest results. The.

Up to about twenty-five years ago, virtually the entire field of microscopy could be overseen and even practized by any active research worker. The rapid evolution which microscopy in its broadest sense has since undergone and which has contributed greatly to our insight in many fields of biological science and medicine has, however, lead to a progressive specialisation. Both experienced investigators in clinical and biological laboratories and post graduate students, confronted with a limited number of microscopic tech niques in their daily research work, have increasing difficulty in keeping (or obtaining) a general idea of the many time-honoured and new possibilities which microscopy has to offer. This book has been written with the aim of presenting general informa tion on light microscopic techniques, at a level somewhere in between booklets like those provided by microscope manufacturers (which are often too much focussed on the production program of a particular make) and very advanced treatises with a thorough mathematical treatment of all phenomena concerned. The physically oriented texts moreover often do not sufficiently take into account the practical situation in a medical or biolog ical laboratory; on the other hand, the value of really understanding what one is doing in using a microscopic technique is often underestimated. At tempt has been made, therefore, to present sufficient background informa tion necessary for a rational application of the different microscopical tech niques in their mutual relationship."

This publication contains the proceedings of a seminar held in Germany (Fed. Rep.) on May 4 -6, 1977, under the auspices of the Commission of the European Communities, as part of the EEC programme of co ordination of research on beef production. The programme was drawn up by a combined scientific working group on Genetics and Selection and on Nutrition and Management on behalf of the Beef Production Committee. The working group consisted of Professor Dr. Neimann S rensen (Denmark, Chairman), Dr. Brolund Larsen (Denmark), Mr. Boccard (France), Dr. H. de Boer (Netherlands), Priv.-Doz. Dr. B. Hoffmann (Germany, Fed. Rep.), Professor Dr. H.J. Langholz (Germany, Fed. Rep.), Dr. J.W.B. King (UK), Mr. R. Jarrige (France), Mr. B. Vissac (France), Professor Dr. A. Romita (Italy), Professor Dr. E.P. Cunningham (Ireland), Mr. P. L'Hermite (CEC) and Dr. J.C. Tayler (Scientific Adviser to CEC). The subject chosen for this seminar was drawn from the list of priorities in research objectives drawn up in 1973 by members of a committee (now the Standing Committee on Agricultural Research, ( PRA). One of the functions of this series of seminars was to summarise and update the information available on the selected subjects and to discuss future needs for research, so as to assist the Commission in evaluating the probable impact of research on agri cultural production within the Community."

J.F. Crul The topic Legal Aspects of Anaesthesia is still rarely treated in book publications, but deserves increasing attention as more cases of litigation occur each year and anaesthetists also become more aware of the legal structure within which they practice their profession. I am happy to have been able to obtain the cooperation of experts in this field from various European countries. The contributing authors in this book come from both the anaesthesia and surgery side as well as from the jurisprudential background. As these two fields have their own professional jargon we have been very careful in using definitions, avoiding that a specific term might lead to misunderstanding and confusion. The international authorship did not facilitate this task. The subject of this book was also the topic of a meeting of the European Academy of Anaesthesiology held at the French Study Center, La Suquette, Saint Vincent Ie Palue~ held three years before publication of this book. The organizers G. Barrier, J.F. Crul, and J. Lassner felt the need for a book publication presenting the state of the art of anaesthesia and the law in European countries. With the present book this plan has been realized. During the meeting many subjects were thoroughly discussed and the editor considered a number of them as very informative and therefore these were included in this book.

This book, the first in an annual series, written by academicians scientists, philosophers and others-is not intended exclusively for an imal welfarists and conservationists. Since it is written* by scholars, it will appeal to a wide range of academic and professional readers who are involved with animals for scientific, economic, altruistic, and other reasons. While this first volume cannot cover the entire spectrum of animal welfare science-related topics, it does, in its diversity of con tributions, demonstrate the multi-faceted and interdisciplinary nature of the subject of this new series. Indeed, animals are as much an integral part of society as we are dependent upon them. The many interfaces between us and the billions of animals under our dominion (as well as the environment upon which the welfare of human and non-human animals alike is ultimately de pendent) have their separate features: trapping and wildlife manage ment; laboratory animal research; whaling and fishing; veterinary practice; agriculture and farm animal husbandry; horse racing and the ownership of animal companions; the propagation of captive wildlife and their preservation in the wild; the use of animals as companions and for the purposes of vicarious entertainment."

This book is a direct result of a symposium held in December 1988, in Washington, DC, honoring Professor Emeritus Miodrag Ristic for his contributions to rickettsial disease research, in general, and, to ehrlichiosis, in particular. He and his colleagues in the United States Army Medical Research Unit brought to the world's attention an epidemic of ehrlichiosis, that occurred in German shepherd dogs during the Vietnam War. The group was able to culture the microorganism Ehrlichia canis and to fulfill Koch's postulates. They eventually developed an indirect immunofluorescent antibody (IFA) test which has been adopted internationally. The same group joined a national effort to decipher another mysterious disease known as Potomac horse fever (PHF). They used the same technology developed by Nyindo in Dr. Ristic's laboratory to isolate, characterize, and again develop a similar IFA test for PHF. Today PHF has been diagnosed, at least serologically, practically across the entire United States, in some provinces of Canada, and reports are beginning to trickle in of its occurrence in European countries. Thus, the etiologic agent of PHF, now named after Professor Ristic, Ehrlichia risticii, historically places this scientist side by side with the other 2 "R's", i. e. , Ricketts and da Rochalima. Ehrlichiosis is not limited to domestic animals. Sennetsu rickettsiosis, long known by Japanese scientists as an imitator of "infectious mononucleosis," was subsequently shown to be caused by an ehrlichial agent, through collaborative efforts among the United States Army, the University of Illinois, and Dr.

Recent advances in electron microscopy have opened up new dimensions and perspectives in the field of morphology, and these are presently being integrated with biochemical and physiopathological phenomena occurring in cells, tissues, and organs. Methods such as freeze-fracture, freeze-etching, scanning, and high-voltage electron microscopy have contributed immensely to this progress, as well as to the study of smooth muscle tissue and contractile cells in general. The articles composing this book have been selected and edited with the purpose of updating and reviewing the most important aspects of smooth muscle cells as revealed by the integration of these submicroscopic techniques. The chapters of this volume have been prepared by some of the most authoritative experts in the discipline. Therefore each article not only offers the reader a concise review of the specific topic, but also seeks to highlight areas that require further investigation. Much of the volume is presented in an illustrative format so as to emphasize the remarkable results obtainable by the combination of the aforementioned methods, which allow a better appreciation of smooth muscle structure and ultrastructure. This volume, like others in the series, is intended not only for researchers in the field, but also for graduate students of histology, embryology, anatomy, physiology, and pathology in both medical and veterinary colleges. My hope is that this book will prove to be a valuable academic resource to the audience of the world in this fascinating and expanding field.

The 5th International Symposium on Microbial Growth on C Compounds was held at the Biological 1 Center of the University of Groningen, Haren, The Netherlands, 11-16 August 1986. The meeting attracted well over 200 participants from 15 countries. This volume contains the formal presentations made at that time, which, because of the breadth of topics covered, were divided into seven sections of related papers. This meeting, under the chairmanship of Wim Harder, was both scientifically and socially very successful. This success cannot only be credited to the main presentations, but also to the well cared for 121 poster presentations, whereof the abstracts have been published separately. The series of Symposia will be continued in 1989, in the Federal Republic of Germany. We wish to acknowledge the invaluable help of Joke Daniels, Roberta Stroer-Schneider, Karin Uyldert, Hansje Bartelson and Josine van Verseveld-Stroer, who retyped the manuscripts resulting in a uniform presentation of these proceedings.

The success of a scientific workshop depends on a delicate blend of many types of ingredients. Most important is to select a provocative topic which is at the forefront of a current investigative study. Coupled together with a relatively small but distinguished group of active research scientists known for their continued record of contributing significant findings, one has the firm foundation for an exciting and rewarding investment of time and effort. This was the setting for the first workshop organized by the European Association for Veterinary Pharmacology and Toxicology. Ruminants have been domesticated for many centuries and have served mankind as a source of dairy products, meat, wool and power. The ruminant stomach has long been - and still is - a major concern for physiologists, pathologists, clinicians and pharmacologists. This workshop was organized and convened in an attempt to strengthen the basic science of the ruminant stomach, as it applies to an economically important group of mammals. To achieve this, various topics were covered by specialists which ensured presentation of new data, followed by discussions. In this book, reviews are presented on the different topics: motility (control and regulation, neurotransmitters and endogenous substances involved); flow of digesta (comparative aspects, role of content and metabolites); food intake; rumen metabolism (chemical manipulation, metabolism of xenobiotics and drugs); pharmacology of forestomach motility and, the reticulo-rumen as a pharmacokinetic compartment.

This publication is the Proceedings of a workshop held at the Royal Veterinary and Agricultural University in Copenhagen, Denmark on 4th - 6th February, 1980, sponsored by the Commission of the EUropean Communities (CEO) as a part of the programme of coordination of agricultural research in the field of animal pathology. The CEO wishes to thank those who took responsibility for the organisation of the workshop, those who presented the papers, and all participants. VII CONTENTS SESSION I METHODOLOGY I MONITORING PASTURE INFECTIVITY AND PASTURE CONTAMINATION WITH INFECTIVE STAGES OF Dictyocaulus viviparus 3 R. J. Jcentsrgensen THE CORRECT HANDLING OF FAECAL SAMPLES USED FOR EXAMINATION OF Dictyocaulus viviparus LARVAE 11 H. J. W. M. Cremers SOME EFFECTS OF STORAGE ON THE RECOVERY OF Dictyocaulus viviparus LARVAE FROM FAECES 17 M. T. Fox EXPERIENCES WITH OUR TECHNIQUES FOR THE RECOVERY OF NEMATODE LARVAE FROM HERBAGE H. -J. Burger 25 A TECHNIQUE FOR THE RECOVERY OF INFECTIVE TRICHOSTRONGYLE LARVAE FROM SOIL 31 K. Bairden, J. L. Duncan and J. Armour SESSION I METHODOLOGY II A MODIFIED AND SIMPLE MCMASTER TECHNIQUE 45 Sv. Aa. Henriksen COMPARISON OF TECHNIQUES FOR ASSESSMENT OF THE CONTAMINATION OF PASTURE HERBAGE WITH INFECTIVE NEMATODE LARVAE 51 J. -P. Raynaud and L. Gruner RECOVERY OF Ostertagia FROM THE BOVINE ABOMASAL MUCOSA BY IMMERSION IN WARM NORMAL SALINE 69 N. E. Downey CONTROLLED/CRITICAL TESTS IN THE EVALUATION OF ANTHELMINTIC ACTIVITY 75 D. D{

The welfare of domestic poultry. particularly those kept under intensive housing conditions is a subject ~n which many. often divergent and conflicting views. are held. This divergence, may be the result either of insufficient knowledge of the facts of particular poultry husbandry systems or a differing interpretation of those facts. With regard to poultry and the laying hen in particular. there is a need to gather together a basic knowledge of avian behaviour in all the circumstances and systems of husbandry under which birds are presently being kept. That knowledge should lead to the development of interpretative and hopefully predictive theories which in turn will allow us to meet the recommendations of Article 3 of the Council of Europe Convention on the Protection of Animals Kept for Farming Purposes: 'that animals shall be housed, provided with food. water and care in a manner which - having regard to their species and to their degree of development. adaptation and domestication - is appropriate to their physiological and ethological needs in accordance with established experience and scientific knowledge'. In furtherance of those objectives a small group of specialists in poultry physiology and ethology. both from within and outside the European Community met by invitation of the Commission in Luxembourg between 11 and 13 March 1980. Their objective was to discuss what is already known. and can be agreed on. of the normal physiology and ethological range of the laying hen.

Animal production systems have changed dramatically over the last two decades. Knowledge of energy metabolism and environmental physio logy has increased as appears from many textbooks on these disciplines. The contents of the symposia on energy metabolism of farm animals show this and they have initially focussed on feed evaluation and later on com parative aspects of energy metabolism. They show part of the progress being made. Application of knowledge of energy metabolism for animals has a long history since Lavoisier. In addition to this, studies about the environ mental requirements of animals have shown that we are still far from ac curate assessment of these requirements in terms of nutrients and ener gy. I n model studies on energy metabolism researchers have recognized the interaction between the environment and the energy requirements of animals. Estimation of energy requirements has been done in physiolo gical, physical and behavioural studies. The impact of conditions as en countered by animals in various production systems has been approached from different viewpoints related to these different disciplines. In addi tion, various kinds of infections (bacterial, parasitic: subclinical, clini cal) have been evaluated only recently with regard to their effect on pro tein and/or energy metabolism and thus on production. People working in the field of feed evaluation have defined how che mical and physical properties of nutrition infiuence energy to be derived for maintenance and production.

The transport of farm livestock was the subject of the seminar held from 7 - 8 July 1981 at the Commission of the European Communities (CECl, Directorate General for Agriculture, Brussels as part of the work of the Division Coordinating Agricultural Research. The aims of the seminar were to examine the knowledge available on how the physiology and behaviour of animals may change during transport; to consider the significance of these changes in relation to welfare and economics and to assess those actions which as experimental projects or observational studies might be proposed to fill the most important gaps in our knowledge of the welfare of farm animals during transport. A number of conclusions can be drawn from the proceedings: 1. Much knowledge is available from both scientific observations and practical experience which could be used to improve the transport of livestock. Methods of loading, the construction of vehicles, ships, crates and aircraft could benefit from the application of existing knowledge. It is less clear whether it is best to concentrate on disseminating existing knowledge by education and advice or to contemplate more regulations. 2. Losses by down-grading at slaughter can largely be attributed to the ways in which animals are transported and handled.

Infertility is a problem for the couple and evaluation of the couple is preferable in a clinic where both males and females may be seen together. The contribution to care by the urologist and the gynaecologist each with an interest in infertility is different but complementary. My appreciation of this was sharpened between 1976 and 1985 during my association with the World Health Organization's Spe cial Programme of Research Development and Research Training in Human Reproduction at the meetings of the Steering Committee of its Task Force on Infertility. The deliberations of this group were aimed at developing a protocol for investigation of the infertile couple and it became apparent to me as a gynaecologist how important is the precise characterization of the male partner's role to the formulation of the management plan for the infertile couple. To improve the quality of our evaluation of the male partner our techni cal staff were trained in the semen methodology of the laboratory of Dr F Com haire of Ghent and we were joined by Dr CLR Barratt, male reproductive biologist. It seemed appropriate to share our enthusiasm for clinical andrology, so after discussion with Dr Comhaire and Professor T Glover, an eminent male reproductive biologist, then of Brisbane, who planned a sabbatical to his native was arranged for 1 and 2 May 1986. Professor Glover en Yorkshire, a meeting of the meeting."

Acid-Base and Electrolyte Handbook for Veterinary Technicians provides an easy to understand yet comprehensive approach to acid-base and electrolyte balance. * Covers the physiology of fluids and their effect on acid-base and electrolyte balance * Offers detailed information on managing acid-base and electrolyte derangements in disease * Includes access to a companion website with case studies and multiple choice questions

It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics.

Molecular farming has been hailed as the "third wave" of genetically-modified organisms produced through biotechnology for the bio-based economy of the future. Unlike products of the first wave, such as herbicide resistant crop plants, which were perceived to benefit only the farmers who used them and the agrochemical companies who developed them, products of molecular farming are designed specifically for the benefit of the consumer. Such products could be purified from food or non-food organisms for a range of applications in industry, as well as animal and human health. Alternatively, the products of this technology could be consumed more directly in some edible format, such as milk, eggs, fruits or vegetables. There is a rapidly-growing interest Qn the part of the public as well as in the medical community in the role food plays in health, especially in the immunophysiological impact of food over and above the role of basic nutrition.

A heartwarming love letter to the places, people and creatures of Yorkshire by the county's beloved vet, Channel 5's Peter Wright. The Yorkshire Vet takes us on an enchanting journey through the hidden gems of the most beautiful county in the world, sharing charming tales of his life in Thirsk as well as fascinating nuggets of local history. Packed with engaging tales of the animals, people and places around him, Peter Wright looks back on the bygone ways of his childhood, while also exploring the fusion of tradition and modernity that characterises the Yorkshire countryside today. Peter's passion for nature shines through on every page, as he explains why our environment is so important - and what we can do to protect it for future generations.