The Zebrafish: Disease Models and Chemical Screens, Fourth Edition, the latest volume in the Methods in Cell Biology series, looks at methods for analyzing genetics, genomics, and transcriptomics of zebrafish. Chapters cover such topics as gene-trap mutagenesis, genetic screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, developmental epigenetics, and the zebrafish interactome. * Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies* Includes chapters written by experts in the field* Contains cutting-edge material on the topics discussed

Molecular Biology of Placental Development and Disease, the latest volume in the Progress in Molecular Biology and Translational Science series, focuses on placental development and disease.

Anaerobiosis and Stemness: An evolutionary paradigm provides a context for understanding the many complexities and evolutionary features of stem cells and the clinical implications of anaerobiosis stem cells. Combining theoretical and experimental knowledge, the authors provide a broad understanding of how the absence or low concentration of oxygen can play an influential role in the maintenance and self-renewal of stem cells and stem cell differentiation. This understanding has clinical implications for the fields of regenerative medicine, cancer biology and transplantation, as well as cell engineering and cell therapy. Anaerobiosis and Stemness is an important resource for stem cell and developmental biologists alike, as well as oncologists, cancer biologists, and researchers using stem cells for regeneration.

An expanded, up-to-date resource for students and researchers, "The Dictionary of Cell & Molecular Biology, Fifth Edition," provides clear and concise definitions needed for laboratory study. With over 12,000 entries and a breadth of terms across bioscience, including plant molecular biology, microbiology and biotechnology, this revision reflects the changes in the research over the past few years. The latest edition of "The Dictionary of Cell and Molecular Biology" maintains its outstanding reputation as an authoritative, clear, and accessible reference with short, cross-referenced definitions of important technical terminology.
Thoroughly revised and expanded by over 20% with over 12,000 entries in cellular and molecular biologyIncludes expanded coverage of terms, including plant molecular biology, microbiology and biotechnology areasConsistently provides the most complete short definitions of technical terminology for anyone working in life sciences todayFeatures extensive cross-references Provides multiple definitions, notes on word origins, and other useful features

This volume of "Methods in Cell Biology" is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish.
* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* This volume concentrates on Disease Models and Chemical Screens

"International Review of Cell & Molecular Biology" presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth.

* Authored by some of the foremost scientists in the field * Provides up-to-date information and directions for future research * Valuable reference material for advanced undergraduates, graduate students and professional scientists

Living cells are constantly sensing environmental changes, and their abilities to sense these changes and adapt to them are essential for their survival. In bacteria, histidine kinases are the major sensors for these environmental stresses, enabling cells to adapt to new growth conditions.
Written by leading experts in the field, this book provides an up-to-date and comprehensive review on the structure and function of histidine kinases. It also provides extensive information on the physiological roles of histidine kinases in bacteria and eukaryotes.
An an essential reference for cell biologists, microbiologists, molecular biologists, and biochemists interested in signal transduction. Experimental biologists and pharmacologists studying signal transduction systems in living organisms will also find it a valuable research tool.
Key Features
* The first comprehensive book on the roles of histidine kinases in cells
* 23 in-depth chapters written by leading experts in the field
* Describes the most recent advances in the field of signal transduction

This volume and its companion Volume 351 will supplement Volume 194 of MIE. The guides are specifically designed to meet the needs of graduate students and postdocs as well as researchers. Whether an established researcher or newcomer to the field, these volumes will contain all the up-to-date methods needed to study "Genes in Yeast." Procedures are included to enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information will be given for proven procedures that can be used as a guide for developing protocols in a number of disciplines.

Containing a comprehensive collection of convenient and quantitative methods for studying centrosomes, spindle pole bodies and related organelles, this text is a valuable resource for researchers and others interested in studying the role of these organelles in cell replication. Chapters outlining the role of these organelles in other cell functions are also included, and a wide variety of experimental systems for analyzing these organelles are presented. Detailed protocols for experiments are contained in each chapter for researchers to perform in their own labs. This volume outlines key methodologies used to analyze centrosomes and spindle pole bodies, their replication, and reproduction in the clear, well-illustrated style of the METHODS IN CELL BIOLOGY series.

The data reference books for practicing scientists present key information for a major subject in one place. This edition has been divided into two parts, recombinant DNA and gene analysis, comprising a compendium of up-to-date methods available in molecular biology. Covering enzymes used for restriction, methylation and modification of nucleic acids, cloning vectors, hosts, genomes and genes as well as techniques such as blotting and hybridization, DNA sequencing, PCR methodology, labelling and separation of nucleic acids.;The edition also provides information on general chemicals and reagents and safety considerations.

In the eight years since the original publication of Molecular Biology Labfax, there has been a vast proliferation of molecular biology techniques. The Second Edition has been divided into two parts: Recombinant DNA, and Gene Analysis. Together they comprise a comprehensive collection of the most up-to-date methods available in molecular biology. This second volume of the two-part Second Edition provides key information on nucleic acid blotting and hybridization, DNA sequencing, PCR, labelingnucleic acids, electrophoresis, centrifugation, chemicals and reagents, and safety. Molecular Biology Labfax will be essential for scientists of all disciplines within the life sciences who use molecular biological techniques.
Key Features
* Provides most up-to-date information on techniques, sources, and equipment for anyone using molecular biological techniques
* Clearly presented information
* Divided into two parts which together comprise a complete collection of molecular biological methods

How do you keep track of basic information on the proteins you work with? Where do you find details of their physicochemical properties, amino acid sequences, gene organization? Are you tired of scanning review articles, primary papers and databases to locate that elusive fact?
The Academic Press FactsBook series will satisfy scientists and clinical researchers suffering from information overload. Each volume provides a catalog of the essential properties of families of molecules. Gene organization, amino acid sequences, physicochemical properties, and biological activity are presented using a common, easy-to-follow format. Taken together they compile everything you want to know about proteins but are too busy to look for. The Chemokine FactsBook contains more than 40 entries on chemokines, and chemokine receptors from human or other origin, including IL-8, MCP-1, C5-a, RANTES, Lymphotactin, and CC CKR-1.
The text provides information on tissue sources, target cells, physicochemical properties, transcription factors, regulation of expression in disease, receptor-binding characteristics, gene structure and location, amino acid sequences, and accession numbers and references.
Key Features
* Contains over 40 entries on chemokines and chemokine receptors from human or other origin, including:
* IL-8
* MCP-1
* C5-a
* RANTES
* Lymphotactin
* CC CKR-1
Entries provide information on:
* Tissue sources
* Target cells
* Physicochemical properties
* Transcription factors
* Regulation of expression
* Expression in disease
* Receptor-binding characteristics
* Gene structure and location
* Amino acid sequences
* Database accession numbers
* References

DNA sequence specificity is a sub-specialty in the general area of molecular recognition. This area includes macromolecular-molecular interactions (e.g., protein-DNA), oligomer-DNA interacitons (e.g., triple strands), and ligand-DNA interactions (e.g., drug-DNA). It is this latter group of DNA sequence specificity interactions that is the subject of Volumes 1 and 2 of "Advances in DNA Sequence Specific Agents." As was the case for Volume 1, Part A also covers methodology, but in Volume 2 we include calorimetric titrations, molecular modeling, X-ray crystallographic and NMR structural studies, and transcriptional assays. Part B also follows the same format as Volume 1 and describes the sequence specificities and covalent and noncovalent interactions of small ligands with DNA.
This volume is aimed in general at scientists who have an interest in deciphering the molecular mechanisms for sequence recognition of DNA. The methods have general applicability to small molecules as well as oligomers and proteins, while the examples provide general principles involved in sequence recognition.

Research on the cytochrome P450 family of genes has traditionally been dominated by forms participating in drug metabolism. This has occurred in spite of early discovery of steroid hydroxy lase P450 cytochromes in the adrenal gland. More recently, contributions on the characterization and regulation of P450 cytochromes involved in biosynthetic reactions have been found at the international meetings on cytochrome P450 and in the several books on the field. Key recognition that P450 cytochromes should be recognized in a physiological context was provided by an international meeting in Jerusalem in 1991 and the subsequent publication of the proceedings in the "Journal of Steroid Biochemistry and Molecular Biology" (43, number 8, 92). Like this meeting, this book seeks to place equal weight on the physiological processes that are controlled by the products of reactions at usually very selective cytochrome P450 forms. Each of the authors was asked to discuss the molecular regulation of these P450 forms. Each of the authors was asked to discuss the molecular regulation of these P450 forms in the light of these physiological processes. In some cases the physiological role of the cytochrome P450 and even the natural substrate are unresolved, but a pattern of strong endocrine regulation is indicative of a hidden function. As more and more low abundance P450 genes are uncovered, the need to address potential physiological activities becomes more pressing. It is almost infinitely more difficult to identify a physiological substrate than to clone a new form.

This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.
Key Features
* A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies
* Includes overviews of
* Optical probes and reagents
* Fabrication and use of ion-selective microelectrodes
* Use of NMR spectroscopy
* Ionophores

A timely book for DNA researchers, Automated DNA Sequencing and Analysis reviews and assesses the state of the art of automated DNA sequence analysis-from the construction of clone libraries to the developmentof laboratory and community databases. It presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted. By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied with instrument and reaction kits and the finalized data presented in the research literature. It will be an invaluable aid to both small laboratories that are interested in taking maximum advantageof automated sequence resources and to groups pursuing large-scale cDNA and genomic sequencing projects.
* The field of automation in DAN sequencing and analysis is rapidly moving. Hovever, as the technology becomes commonplace, those applying the techniques involved to their research fields need a text which both expands on the protocols supplied by manufacturers with their instruments and explains how to utilise the data produced. This book fulfils those needs, reviews the history of the art and provides pointers to future development.

The technique of in situ hybridization is now vital to molecular biologists and their understanding of the pathophysiology of cellular functions. This practical guide covers all aspects of in situ hybridization, describing: practical procedures and protocols; the scientific background; areas of application; and the limitations of the technology. This edition has been completely rewritten to take into acocunt the many advances in the seven years since its initial publication.

Measuring metabolic rates is central to important questions in many areas of scientific research. Unfortunately these measurements are anything but straightforward, and numerous pitfalls await the novice and even the experienced investigator.
Measuring Metabolic Rates de-mystifies the field, explaining every common variation of metabolic rate measurement, from century-old manometric methods through ingenious syringe-based techniques, direct calorimetry, aquatic respirometry, stable-isotope metabolic measurement and every type of flow-through respirometry. Each variation is described in enough detail to allow it to be applied in practice. Background information on different analyzer and equipment types allows users to choose the best instruments for their application. Respirometry equations - normally a topic of terror and confusion to researchers - are derived and described in enough detail to make their selection and use effortless.
Vital topics such as manual and automated baselining, implementing multi-animal systems, and the correct analysis and presentation of metabolic data are covered in enough detail to turn a respirometry neophyte into a hardened metabolic warrior, ready to take on the task of publication in peer-reviewed journals.

This new volume, number 123, of "Methods in Cell Biology" looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications.

The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.
Covers sections on model systems and functional studies, imaging-based approaches and emerging studiesChapters are written by experts in the fieldCutting-edge material"

Resident Stem Cells and Regenerative Therapy: Sources and Clinical Applications, Second Edition presents the main findings to date and the important factors to be considered when contemplating resident stem cells in regenerative therapies. Chapters on cardiac, brain, neural, liver, kidney, skeletal muscle, bone, pancreatic, skin and lung resident stem cells will assist in defining the level of success that has been achieved and the direction for the road ahead. With contributions from leading laboratories, open questions related to resident stem cells and regenerative therapies will also be presented for debate. In the last several decades, stem cells have greatly impacted the scientific and lay communities, providing huge advances in the treatment of devastating human diseases, including myocardial infarction, diabetes, muscular dystrophy, cystic fibrosis, cirrhosis, and osteoporosis. Alongside debates of induced pluripotent stem cells and embryonic stem cells has been the discovery of adult stem cells in many different tissues. While these organ resident or progenitor stem cells offer prospects to contribute to tissue regeneration, they also present challenges because of the complexity of organ structures.

Viral Vectors in Cancer Immunotherapy, Volume 379 in the International Review of Cell and Molecular Biology presents the latest on cancer immunotherapy and how it has transformed cancer treatment through advances in immune checkpoint inhibitors and adoptive cell therapy. Chapters in this new release include Past, present and future of viral vectors in cancer immunotherapy, Alphaviruses in cancer immunotherapy, Adenoviral-based cancer gene therapy, Armored modified vaccinia Ankara in cancer immunotherapy, Strategies of Semliki Forest virus in immuno-oncology, Maraba virus in cancer immunotherapy, Oncolytic viruses in hematological malignancies, Oncolytic virus for cancer therapies: Overview and future directions, and more. The use of genetically modified viruses allows the expression of pro-inflammatory molecules, while the immune system receives danger signals from the viruses themselves. In some cases, the virus can also induce tumor cell death. This book will review advances in virus-based cancer immunotherapy in both solid tumors and hematologic malignancies.

This special volume titled The Cardiovascular Glycocalyx in Health and Disease has the goal of giving an updated perspective of the glycocalyx function through the cardiovascular system and its integration in whole system physiology. This volume will address roles of the glycocalyx at the membrane and cellular level and its implications to overall cardiovascular function and disease progression. Further, pharmacological targeting of the glycocalyx will be addressed as an important mechanism for diagnosis and therapeutic interventions.

Ionizing Radiation and the Immune Response, Part B, Volume 378 reviews the latest knowledge on the immune response induced by ionizing radiations. Specific chapters in this new release include NK functions in radio-induced immune response, TRT and immune response, Radio-induced immune response and lipid metabolism, Effect of protons and heavy ions on immune response, Effect of flash therapy and mini beam on immune response, Radio-induced lymphopenia, CT to potentiate radio-induced immune response, Impact of RT on healthy tissues (inflammation), Radio-induced macrophagic response, To use nanoparticles and ionizing radiations to modulate immune response: opinion of the chemist, biologist and clinician, and much more. Other sections cover the Role of Dendritic cells in radiation-induced immune response, the Relationship between the tumor microenvironment and the efficacy of the radiotherapy/immunotherapy combination, and Biomarkers of radiation induced response to optimize radio-immunotherapy combination.

Advances in Biomembranes and Lipid Self-Assembly, Volume 37 provides a comprehensive compilation of recent developments in a field that is in a state of rapid growth as new experimental and theoretical techniques are used on many problems, both old and new. Topics covered include related applied areas, such as atmospheric science, astrophysics, surface physics, and laser physics, with timely articles written by experts. New chapters in this updated release include Engulfment of particles by vesicles containing curved membrane proteins coupled with active cytoskeletal forces, Interaction of cells with different types of nanostructured surfaces, Solid-supported lipid bilayer formation by solvent exchange, and more.