Plants have a very specific and efficient mechanism to obtain, translocate and store nutrients from the surrounding environment. The precise mechanism that helps a plant in nutrient translocation from root to shoot also, in the same way, transfers and stores toxic metals within their structure. Metal toxicity generally causes multiple direct or indirect effects on plants, affecting nearly all of their physiological functions. Plant tolerance to heavy metals depends largely on plant efficiency in uptake, translocation and sequestration of heavy metals in specific cell organelles or specialized tissues. The main purpose of this book is to present a holistic view of the recent advancement in the field of accumulation and remediation using plants, the green solar powered alternative to ameliorate heavy metal from the polluted environment. The key features of the book are related to metal transporters and metal accumulation mechanisms under heavy metal stress in plants, plant transcriptional regulation and responses under metal contamination, multiple toxic metal contaminations and its phytoremediation approaches etc. Based on the advancement of research in recent years, the information compiled in this book will bring an in-depth knowledge on the bioaccumulation of metals, their transportation in natural conditions or genetically modified plants and their strategy to cope with the toxicity to survive in the hostile environment.

This book describes the unique characean experimental system, which provides a simplified model for many aspects of the physiology, transport and electrophysiology of higher plants. The first chapter offers a thorough grounding in the morphology, taxonomy and ecology of Characeae plants. Research on characean detached cells in steady state is summarised in Chapter 2, and Chapter 3 covers characean detached cells subjected to calibrated and mostly abiotic types of stress: touch, wounding, voltage clamp to depolarised and hyperpolarised potential difference levels, osmotic and saline stress. Chapter 4 highlights cytoplasmic streaming, cell-to-cell transport, gravitropism, cell walls and the role of Characeae in phytoremediation. The book is intended for researchers and students using the characean system and will also serve as an invaluable reference resource for electrophysiologists working on higher plants.

Plants cannot move away from their environments. As a result, all plants that have survived to date have evolved sophisticated signaling mechanisms that allow them to perceive, respond, and adapt to constantly changing environmental conditions. Among the many cellular processes that respond to environmental changes, elevation of calcium levels is by far the most universal messenger that matches primary signals to cellular responses. Yet it remains unclear how calcium, a simple cation, translates so many different signals into distinct responses - how is the "specificity" of signal-response coupling encoded within the calcium changes? This book will attempt to answer this question by describing the cellular and molecular mechanisms underlying the coding and decoding of calcium signals in plant cells.

These proceedings bring together diverse disciplines that study nitrogen fixation and describe the most recent advances made in various fields: chemists are now studying FeMoco, the active site of nitrogenase in non-protein surroundings, and have refined the crystal structure of the enzyme to 1.6 angstroms.

The tomato is commercially important throughout the world both for the fresh fruit market and the processed food industries. It is grown in a wide range of climates in the field, under protection in plastic greenhouses and in heated glasshouses. Genetic, physiological and pathological investigations frequently adopt the tomato plant as a convenient subject. Hitherto, much of the information on tomatoes has been fragmented: tomatoes grown in the field and under protection have been considered separately and the more fundamental findings from research have often failed to reach those involved directly or indirectly in commercial crop production. Similarly, the research scientist is often unaware of the problems of commercial crop production and the possible relevance of his work to the crop. This book is an attempt to rectify that situation. By giving a thorough scientific review of all factors influencing tomato production systems, it is hoped that this book will prove useful to students, researchers and commercial producers alike. It gives the basis for the develop ment of improved cultivars, the formulation of strategies for managing pest, disease and disorder problems and the production of high yields of good quality fruit as well as suggesting important areas for scientific initiatives. The extensive bibliographies provide a comprehensive database for tomato researchers. Such a vast subject could not be covered with authority by anyone author."

The formation of roots is in some respects one of the least fundamentally understood of all plant functions. Propagation by cuttings is the aspect that will occur first to most gardeners and horticulturists, and it is certainly the most useful application. But any observant traveller in the tropics can notice that some trees have the habit of forming roots in the air. Climbers like Cissus bear long fine strings of roots hanging down. Pandanus trees tend to have stout aerial roots issuing from the bases of the long branches, while the tangle of roots around the trunk of many of the Ficus species is characteristic. In Ficus bengalensis, in particular, stout cylindrical roots firmly embedded in the ground from a height of 3 to 5 meters give support to the long horizontal branches, enabling them to spread still further. In the big old specimen at Adyar near Madras, the spread of these branches all around the tree, each with a strong root growing out every few meters, makes a shaded area under which meetings of almost 5000 people are sometimes held. The history of how the formation of roots on stem cuttings was found to be under hormonal control is worth repeating here.

Heme and chlorophyll (Chl) are porphyrins. Porphyrins (also referred to as tetrapyrroles) are essential for life in the biosphere. Chlorophyll catalyzes the conversion of solar energy to chemical energy via the process of photosynthesis. Organic life in the biosphere is made possible by consumption of the chemical energy generated by photosynthesis. Hemes are the prosthetic groups of cytochromes which are involved in electron transport during oxidative phosphorylation and photosynthetic phosphorylation which generate ATP and NADPH. The latter are essential for many cellular functions. Chlorophyll on the other hands catalyzes the process of photosynthesis. Indeed, life in the biosphere depends on the process of photosynthesis which converts light energy, carbon dioxide and water into the chemical energy, required for the formation of food and fiber. Photosynthetic efficiency is controlled by extrinsic factors such as the availability of water, CO2, inorganic nutrients, ambient temperature and the metabolic and developmental state of the plant, as well as by intrinsic factors (Lien and San Pietro, 1975). The most important intrinsic factor is the efficiency of the photosynthetic electron transport system (PETS). Conventional agriculture is one of the few human activities that have not undergone a revolution to join other activities such as overcoming gravity by flying, and landing on the moon, crossing underwater the polar cap, and communicating wirelessly over long distances via electromagnetic waves. We now feel that enough biochemical and molecular biological knowledge has accumulated to render this dream amenable to experimentation. We believe that the time has come to bioengineer chloroplasts capable of synthesizing a short chain carbohydrate such as glycerol at rates that approach the upper theoretical limits of photosynthesis [Rebeiz, C. A. (2010) Investigations of possible relationships between the chlorophyll biosynthetic pathway and the assembly of chlorophyll-protein complexes and photosynthetic efficiency. In: Rebeiz, C. A. Benning, C., Bohnert, H.J., Daniell, H., Hoober J. K., Lichtenthaler, H. K., Portis , A. R., and Tripathy, B. C. eds. The chloroplast: Basics and Applications. Springer. The Netherlands, p 1-24]. In order to achieve this goal a thorough knowledge of the Chl biosynthetic pathway is needed along with knowledge in other domains (Rebeiz 2010). In this context, this monograph is devoted to an in depth discussion of our present knowledge of the Chl biosynthetic pathway. The complexity and biochemical heterogeneity of the Chl biosynthetic pathway and the relationship of this complexity to the structural and biosynthetic complexity of photosynthetic membranes will be emphasized. We will also emphasize in historical perspective, key stages in our understanding of the Chl biosynthetic heterogeneity. The reader should keep in mind that a complex biosynthetic process is only fully understood when it becomes possible to reconstitute in vitro every step of the process. We are not yet at this stage of understanding of thylakoid membrane biogenesis. Considerable progress has been achieved however, in the understanding of numerous facets of the Chl biosynthetic pathway, namely (a) detection and identification of various major and minor metabolic intermediates (b) precursor-product relationships between various intermediates, (c) structure and regulation of many enzymes of the pathway, and (d) the relationship of the Chl biosynthetic heterogeneity to the structural and functional heterogeneity of thylakoid membranes. In addition topics related to the development of Analytical techniques, Cell-free systems, Herbicides, Insecticides, and Cancericides are also discussed.

In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.

Plant innate immunity is a potential surveillance system of plants and is the first line of defense against invading pathogens. The immune system is a sleeping system in unstressed healthy plants and is activated on perception of the pathogen-associated molecular patterns (PAMP; the pathogen s signature) of invading pathogens. The PAMP alarm/danger signals are perceived by plant pattern-recognition receptors (PRRs). The plant immune system uses several second messengers to encode information generated by the PAMPs and deliver the information downstream of PRRs to proteins which decode/interpret signals and initiate defense gene expression. This book describes the most fascinating PAMP-PRR signaling complex and signal transduction systems. It also discusses the highly complex networks of signaling pathways involved in transmission of the signals to induce distinctly different defense-related genes to mount offence against pathogens."

Desiccation tolerance was essential when plants first began to conquer land, roughly 400 million years ago. While most desiccation-tolerant plants belong to basal phylogenetic taxa, this capacity has also evolved among some vascular plant species.

In this volume renowned experts treat plant desiccation tolerance at the organismic as well as at the cellular level. The diversity of ecophysiological adaptations and acclimations of cyanobacteria, eukaryotic algae, mosses, and lichens is addressed in several chapters. The particular problems of vascular plants during dehydration/rehydration cycles resulting not only from their hydraulic architectures, but also from severe secondary stresses associated with the desiccated state are discussed. Based on the treatment of desiccation tolerance at the organismic level, a second section of the book is devoted to the cell biological level. It delineates the general concepts of functional genomics, epigenetics, genetics, molecular biology and the sensing and signalling networks of systems biology involved in dehydration/rehydration cycles.

This book provides an invaluable compilation of current knowledge, which is a prerequisite for a better understanding of plant desiccation tolerance in natural as well as agro- and forest ecosystems where water is one of the most essential resources.

Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on classical light microscopy and histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized electron microscopy techniques, and methods used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by expert researchers in the field and include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

The Molecular Biology ofChloroplasts and a genetic dissection ofphotosynthesis was first Mitochondria in Chlamydomonas is the seventh recognized by Paul Levine. Together with his volume to be published in the series Advances in coworkers, he initiated along-rangegenetic approach Photosynthesis of Kluwer Academic Publishers which proved to be highly successful. It provided (Series Editor: Govindjee). Volume 1 dealtwith The genetic support for the linear Z scheme of Molecular Biology of Cyanobacteria; Volume 2 with photosynthesis and led to the identification ofnew Anoxygenic PhotosyntheticBacteria; Volume 3 with components ofthe photosynthetic electron transfer BiophysicalTechniques in Photosynthesis; Volume 4 chain such astheRieskeproteinofthe cytochrome with Photosynthesis and the Environment; and complex. Volume 6 with Lipids in Photosynthesis: Structure, During the past 20 years, the powerful techniques of molecular biology and genetics, and the Function and Genetics. The main goal ofthis book is to provide a development ofmethods for efficient nuclear and comprehensive overview ofcurrent research with chloroplast transformation of C. reinhardtii have the green alga Chlamydomonas on chloroplast and greatly enhanced the potential ofthis organism as an mitochondrial biogenesis and function, with special experimental system for studying chloroplast emphasis on the assembly and structure-function biogenesis. This has led to impressive advances in relationships ofthe constituents ofthe photosynthetic our understanding of the regulation of chloroplast apparatus.

The progress in photosynthesis research has been transduction and expression of photosynthetic genes quite dramatic during the last two decades. The which occur both in the nuclear/cytosol compartment Nobel prizes awarded to Peter Mitchel (1978), to and in the chloroplast. Several chapters are devoted Johannes Deisenhofer, Hartmut Michel and Robert to the transcription machinery and the two plastid Huber (1988), to Rudolf Marcus (1992) and to Paul RNA-polymerase complexes, to the regulation of Boyer and John Walker (1997) have recognized photosynthesis genes by redox signaling both in directly or indirectly the structural or mechanistic chloroplasts and in the prokaryotic systems, as well discoveries related to the photosynthetic energy as to the sugar sensing mechanisms. Chapters also conversion. Actually, photosynthesis may be the first cover important regulatory aspects imposed by po- biological process described, not only in molecular transcriptional modifications and degradation of terms, but even in atomic terms. mRNA molecules, and the translational regulation Much of the excitement around photosynthesis is mechanisms operating in chloroplasts. based upon the connection between light and life. Part III Biogenesis, turnover and senescence is closely connected to the question of regulation. Light is an elusive substrate that cannot be handled The chapters included emphasize how the c- in the same way as conventional chemical substrates plicated membrane structures, composed of both in biological metabolic reactions."

A shortage of water exists, not only in the arid regions of the world, but even in some moderately humid climates. This situation is a consequence of water require ments for agriculture and industry in amounts greater than the natural surplus. Even in Europe there is increased anxiety over the state of water reserves, and shortages are forecast for the near future if industry continues to expand. During the past 50 years in the United States, water use has increased about twice as fast as the rate of population growth, and shortages have already appeared in some places. The need to conserve declining water resources which has become apparent over the last few decades has led several investigators to conclude that plants with a high rate of transpiration endanger water resources, and the growth of such plants must not be encouraged. Some think that trees withdraw more water from the soil than other plant species and evaporate it excessively through the stomata of leaves. THORNTHWAITE and HARE (1955) explained transpiration on the same thermo dynamic basis as evaporation, and calculated its rate, using DALTON'S law or modifications thereof. In spite ofthe many past and present investigations into the problems of transpiration, the biological aspects of this essential process is still poorly understood."

The refinement of molecular techniques and the development of new probes of the phylogeny of diazotrophs has revealed an extreme biodiversity among the nitrogen fixers, which helps explain the role that nitrogen fixation plays in maintaining life on Earth. The most efficient ecosystems are those where the bacteria are associated with a plant in differentiated organs to benefit crop productivity. Most short-term benefit from fundamental research on nitrogen fixation is likely to result in the improvement of existing nitrogen-fixing symbiotic or associative systems. Longer-term efforts are aimed at extending the nitrogen-fixing capacity to other organisms, including transfer of the genetic information for efficient nitrogen fixation into the plant genome and using current knowledge of microbe-plant interactions to extend symbiosis to cereals and, in particular, to rice. Related challenges in sustainable agriculture and forestry include the creation of new nitrogen-fixing associations. All of these approaches were discussed at the 11th International Congress on Nitrogen Fixation, Paris, France, July 20-25, 1997 and covered in the present proceedings volume.

Abiotic stress has a detrimental impact on the living organisms in a specific environment and constitutes a major constraint to global agricultural production. The adverse environmental conditions that plants encounter during their life cycle not only disturb their metabolic reactions, but also hamper their growth and development on cellular and whole plant levels. These conditions are of great concern, particularly for those countries whose economies primarily rely on agriculture. Under abiotic stresses, plants amalgamate multiple external stress cues to bring about a coordinated response and establish mechanisms to mitigate such stresses by triggering a cascade of events leading to enhanced tolerance.

"Physiological Mechanisms and Adaptation Strategies in Plants under Changing Environment," "Volume 2" displays the ways by which plants utilize and integrate many common signals and subsequent pathways to cope with less favourable environmental conditions." "The book also describes the use of contemporary tools for the improvement of plants under such stressed environments. Concise yet comprehensive, "Physiological Mechanisms and Adaptation Strategies in Plants under Changing Environment," "Volume 2" is an indispensable resource for researchers, students, environmentalists and many others in this burgeoning area of research."

Environmental conditions and changes, irrespective of source, cause a variety of stresses, one of the most prevalent of which is salt stress. Excess amount of salt in the soil adversely affects plant growth and development, and impairs production. Nearly 20% of the world's cultivated area and nearly half of the world's irrigated lands are affected by salinity. Processes such as seed germination, seedling growth and vigour, vegetative growth, flowering and fruit set are adversely affected by high salt concentration, ultimately causing diminished economic yield and also quality of produce. Most plants cannot tolerate salt-stress. High salt concentrations decrease the osmotic potential of soil solution, creating a water stress in plants and severe ion toxicity. The interactions of salts with mineral nutrition may result in nutrient imbalances and deficiencies. The consequence of all these can ultimately lead to plant death as a result of growth arrest and molecular damage. To achieve salt-tolerance, the foremost task is either to prevent or alleviate the damage, or to re-establish homeostatic conditions in the new stressful environment. Barring a few exceptions, the conventional breeding techniques have been unsuccessful in transferring the salt-tolerance trait to the target species. A host of genes encoding different structural and regulatory proteins have been used over the past 5-6 years for the development of a range of abiotic stress-tolerant plants. It has been shown that using regulatory genes is a more effective approach for developing stress-tolerant plants. Thus, understanding the molecular basis will be helpful in developing selection strategies for improving salinity tolerance. This book will shed light on the effect of salt stress on plants development, proteomics, genomics, genetic engineering, and plant adaptations, among other topics. The book will cover around 25 chapters with contributors from all over the world.

What are plant growth regulators? In the title, and throughout the text, we have adopted this expression to describe a population of endogenous molecules and synthetic compounds of similar structure that are be lieved to play important roles in the regulation of plant differentiation and development. For many years, plant scientists have endeavoured to understand the nature and action of plant growth regulators and, as a result, an awesome quantity of written material now exists describing these chemicals and their effects. In this book we have aimed to distil this wealth of information into a more digestible form, and in particular we have focused our attention on a critical appraisal of the literature. The past few years have witnessed a change of emphasis in plant growth regulator research, which has been fuelled by powerful new techniques in molecular and cell biology. Today we can do more than just apply a plant growth regulator and quantify its effects; we have reached an exciting crossroads where plant scientists, molecular bio logists and chemists can pool their expertise and apply it to the out standing problems in this area. The combination of these three disciplines within the book is clear evidence of this. In keeping with a volume of this size, we have assumed that the reader has a sound knowledge of plant physiology and biochemistry. However, wherever possible, we have highlighted useful reviews which provide background information, along with recent publications that have contributed significantly to the literature."

Seaweeds, also known as macroalgae, are among the most important primary producers and act as ecological engineers on rocky coasts of the world s oceans. In addition to their extreme ecological importance they are also of high economic relevance. Complementing available textbooks with its more research-oriented approach, this volume contains 22 chapters by renowned experts, grouped in five parts. In Part I fundamental processes and acclimation strategies of seaweeds towards the abiotic environment are covered. Part II focuses on the multitude of biotic interactions in seaweed communities, and in Part III the reader is introduced to the structure and function of the main seaweed systems of the world. The chapters of Part IV highlight and discuss the effects of global and local environmental changes on seaweeds and their communities. In the final Part V a comprehensive overview of developments in seaweed aquaculture, industrial applications and the overall economic importance of seaweeds is provided. Summarizing the advances in seaweed biology achieved within the last few decades, this book also identifies gaps in the present knowledge and needs for future research."

With contributions that review research on this topic throughout the world, "Oxidative Damage to Plants" covers key areas of discovery, from the generation of reactive oxygen species (ROSs), their mechanisms, quenching of these ROSs through enzymatic and non-enzymatic antioxidants, and detailed aspects of such antioxidants as SOD and CAT.

Environmental stress is responsible for the generation of oxidative stress, which causes oxidative damage to biomolecules and hence reduces crop yield. To cope up with these problems, scientists have to fully understand the generation of reactive oxygen species, its impact on plants and how plants will be able to withstand these stresses.
Provides invaluable information about the role of antioxidants in alleviating oxidative stressExamines both the negative effects (senescence, impaired photosynthesis and necrosis) and positive effects (crucial role that superoxide plays against invading microbes) of ROS on plantsFeatures contributors from a variety of regions globally

This book reviews various aspects of papaya genomics, including existing genetic and genomic resources, recent progress on structural and functional genomics, and their applications in papaya improvement. Organized into four sections, the volume explores the origin and domestication of papaya, classic genetics and breeding, recent progress on molecular genetics, and current and future applications of genomic resources for papaya improvement. Bolstered by contributions from authorities in the field, Genetics and Genomics of Papaya is a valuable resource that provides the most up to date information for papaya researchers and plant biologists.

Our view of plants is changing dramatically. Rather than being only slowly responding organisms, their signaling is often very fast and signals, both of endogenous and exogenous origin, spread throughout plant bodies rapidly. Higher plants coordinate and integrate their tissues and organs via sophisticated sensory systems, which sensitively screen both internal and external factors, feeding them information through both chemical and electrical systemic long-distance communication channels. This revolution in our understanding of higher plants started some twenty years ago with the discovery of systemin and rapid advances continue to be made. This volume captures the current 'state of the art' of this exciting topic in plant sciences.

Chemical reactions and interactions between molecules are commonly considered the basis of life, and thus the biochemical nature of cells and organisms is relatively well recognized. Research conducted in recent years, however, increasingly indicates that physical forces profoundly affect the functioning of life at all levels of its organization. To detect and to respond to such forces, plant cells and plants need to be structured mechanically. This volume focuses on mechanical aspects of plant life. It starts with a consideration of the mechanical integration of supracellular structures and mechanical properties of cellular building blocks to show how the structural integrity of plant cells is achieved and maintained during growth and development. The following chapters reveal how the functioning of integrated plant cells contributes to the mechanical integration of plants, and how the latter are able to detect physical stimuli and to reorganize their own cells in response to them. The mechanical aspects of plant responses to stresses are also presented. Finally, all these aspects are placed in an evolutionary context.

Chloroplast development is a key feature of leaf developmental program. Recent advances in plant biology reveal that chloroplasts also determine the development, the structure and the physiology of the entire plant. The books, published thus far, have emphasized the biogenesis of the organelle, but not the events associated with the transformation of the mature chloroplast to the gerontoplast during senescence. This book, with 28 chapters, is unique because it describes how the chloroplast matures and how it is subsequently transformed to become the gerontoplast during senescence, a process required for nutrient recycling in plants. This book includes a state-of-the-art survey of the current knowledge on the regulation and the mechanisms of chloroplast development.Some of the chapters critically discuss the signaling process, the expression potential of plastid DNA, the interaction of cellular organelles, and the molecular mechanisms associated with the assembly and the disassembly of organellar complexes and finally the modulation of chloroplast development by environmental signals."

Scientists are continually making exciting discoveries concerning the interactions between microbes and plants, interactions which may be damaging, in the case of plant pathogens, or beneficial, as in the case of nitrogen fixation. This new volume in the successful and well received Chapman & Hall Plant-Microbe Interaction series is an exciting and broad-ranging view of the outstanding work being done in this area.