Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.

Plant breeders continue to make significant advances in developing high yield ing, adaptable, disease-free crops. These advances, however, are not realized until an efficient seed production system is in place that rapidly increases geneti cally superior crops and makes them available to the consumer in large quantities at a reasonable cost. Successful seed production requires seed to be genetically pure, free of admixtures, and able to establish rapidly a uniform stand. Seed production is a complex process. Rigorous production criteria are followed by both seed producer and seed companies to ensure that high-quality seed is produced and marketed. These criteria become even more stringent in hybrid seed production. This volume identifies the factors most critical in a successful seed production operation. The fundamental considerations common to all seed crops are established in Part I, Principles of Seed Production. From this founda tion, the practices of seed production are provided in detail in Part II, Seed Production of Specific Crops."

Plant-Microbe Interactions, Volume 2 Volume 1 of this series has made its appearance and dealt forcefully with impor tant current topics in the field of plant-microbe interactions. We believe that the quality of those chapters was high and should serve as a focal point for the state of the art as well as an enduring reference. Volume 2 builds upon these accom plishments. Chapter 1 discusses the fascinating lipo-chitin signal molecules from Rhizo bium, aspects regarding their biosynthesis, and the basis for host specificity. These molecules are a cardinal example of how microorganisms influence plant development and stimulate speculation that they have identified a previously un known aspect of plant hormone activity. Chapter 2 continues the discussion of Rhizobium by considering the trafficking of carbon and nitrogen in nodules. Al though the ostensible advantage of nodules to plants is the fixation of atmos pheric nitrogen, the actual process involved in supplying reduced nitrogen to the plant host is complex."

This timely reference provides an in-depth and up-to-date study of the most important groups of plant secondary compounds. No other work features such detailed diagrams of the biosynthetic pathways leading to the most important groups of secondary metabolites, as well as the structures of major types of compounds, their distribution in various taxonomic groupings of plants, and the evolutionary and ecological roles of these compounds. Plant Secondary Metabolism presents a basic understanding of the origin of the compounds, the nature of the precursors involved, and the basic reactions, mechanisms, and stereochemistry. The origin of groups of secondary metabolites is linked to evolutionary principles, and their biological activity is viewed in a context of chemical ecology. Topics are treated comprehensively, enabling the reader to understand not only a particular group of compounds, but also how each group fits into the whole. In addition, the text allows readers to systematically survey various secondary metabolites and gain a quick working knowledge which can be applied to problems in a particular field.Those researchers and students who will be most intrigued by this publication's broad overview on plant secondary metabolites come from a diverse range of disciplines, including agronomy, anthropology, biochemistry, biology, botany, chemistry, ecology, entomology, food science, forestry, geology, horticulture, pharmacognosy, plant biology, plant sciences, toxicology, and zoology.

In the field of plant analysis there is a confusing variety of methods and procedures, both for digestions and determinations. In many cases the digestion and the subsequent determination are interrelated. For example, a separate digestion is needed for trace elements in order to obtain determinable concentrations. The authors have chosen a design in which the digestion/extraction procedure is described in one chapter together with all determination procedures that may be carried out on that particular digest/extract. All the necessary information (such as standardizations) appears in appendices. As a consequence, several determination procedures are described two or three times, however, each based on a particular digestion or extraction method. Two types of determination procedure are described: manual and automated. Manual procedures are mainly used in research laboratories, whereas automated procedures are more frequently applied in routine laboratories. Both types of determinations can be used freely, provided that appropriate equipment is available. The determination procedures are only for inorganic components, usually elements. Besides, most procedures are designed to give a total content value of the element under consideration, regardless of the chemical structure in which it occurs in the plant. The Plant Analysis Manual is intended for the practicing (agricultural) chemist.

The Monograph deals with the conception, planning, implementation, results and conclusions of the International Witches' Broom Project (IWBP), which was set up in 1985 with the aim of producing an economic management system for witches' broom disease of cocoa. The contributions of the various sponsors, and the roles played by the participating organizations and scientists are described in the introductory chapter. Chapter 2 provides a review of what was, and what was not known from published literature about the cocoa witches' broom pathosystem in 1989. The scope of the project and the approaches used are covered in Chapter 3, while Chapters 4 to 13 report on the field studies themselves in detail. The recent appearance of witches' broom in the important cocoa area of Bahia in Brazil is described in Chapter 14, before disease management recommendations are summarised and future prospects considered in the closing chapters. The many man-years of field research in the IWBP in a total of six countries generated much useful information which was analyzed both in the individual countries and collectively. Even with a document of this size, certain information and analyses with less direct relevance to disease management had to be omitted. It is expected that more detailed treatments of certain aspects will emerge in scientific papers, and further analyses will be undertaken."

The book will be a broad and comprehensive look on Jatropha until the details since the book is being contributed by international experts worldwide that have already published works in the international press of Science. Illustrations, tables geographic maps, GPS location, etc are added by each contributors according to the feeling they have concerning what they think their contribution should be. This book will benefit the scientific community immensely. Being aware of any challenges related to Jatropha, i.e. (i) its economy in Asia (India, China) and South America (Brazil), (ii) basics of biofuel technology, (iii) physiology, (iv) farming, (v) byproducts, (vi) biotechnology, (vii) genetic resource (germplasm) and their benefit for the crop by genetic transfer, (viii) genetic map, (ix) comparative genetics, (x) genomics. Breeders and technologist will have access to a complete digested view on Jatropha to decide where and how they should move on with their investigations.

Rapid advances have taken place in various aspects of reproductive biology during the last decade. These advances have centered around several organ systems that comprise the reproductive system and encompass molecular events and structure-function relationships. It becomes important to review these advances in knowledge, at periodic intervals, with respect to feedback systems and regulatory loops that control reproductive processes in vivo. Towards this end, a workshop entitled "Functional Correlates of Hormone Receptors in Reproduction" sponsored by the National Institute of Child Health and Human Development and the Reproductive Biology Study Section of the Division of Research Grants, National Institutes of Health was held in October 1980. The proceedings of the workshop were published by Elsevier Biomedical/New York. This workshop was followed by two workshops sponsored by the Reproductive Biology Study Section of the Division of Research Grants, National Institutes of Health entitled "Role of Peptides and Proteins in Control of Reproduction" in February 1982 and published by Elsevier Biomedical and "Molecular and Cellular Aspects of Reproduction" in October 1985 and published by Plenum Press. It was, therefore, timely to review the current state of knowledge regarding the regulation of ovarian and testicular function by bringing together scientists working in separate and discrete aspects of reproduction to review the functional implications of their research on the regulation of function within the same tissue and also in relationship to feedback systems and regulatory loops with other tissues.

In a field of mature bananas, plants can be seen at all stages of vegetative growth and fruit maturity, providing a fascination for anyone who has an interest in growing crops. Banana farmers in the tropics can harvest fruit every day of the year. The absence of seasonality in production is an advantage, in that it provides a continuity of carbohydrate to meet dietary needs as well as a regular source of income, a feature that perhaps has been under-estimated by rural planners and agricultural strategists. The burgeoning interest in bananas in the last 20 years results from the belated realization that Musa is an under-exploited genus, notwithstanding the fact that one genetically narrow group, the Cavendish cultivars, supply a major export commodity second only to citrus in terms of the world fruit trade. International research interest in the diversity of fruit types has been slow to develop, presumably because bananas and plantains have hitherto been regarded as a reliable backyard source of dessert fruit or starch supplying the needs of the household, and in this situation relatively untroubled by pests, diseases or agronomic problems.

Nematode interactions are important biological phenomena and of great significance in agriculture. It is a fascinating subject which is multidisciplinary by nature, and concerns any scientist involved with plant health. There have been marked advances in our knowledge of various aspects of the subject in the last two decades. This study area has been the subject of several reviews, but there was no exclusive text on the subject. This has stressed the need to document the information, developing a unifying theme which treated nematode interactions in a holistic manner. This book is about the inter action of plant-parasitic nematodes with other plant pathogens or root symbionts, the nature of their associations, their impact on the host and con sequential interactive effects on the involved organisms. Since nematodes are at the centre of the theme, the responsibility of understanding of other plant pathogens dealt with in this book is largely delegated to the reader. I have limited the book content to interactions with biotic pathogens and root symbionts only, for various reasons. The book embodies 16 chapters, and attempts to present balanced infor mation on various aspects of nematode interactions with other plant pathogens and root symbionts. Some chapters describe general aspects of the subject. Interactions of nematodes with specific groups of organisms are addressed in the remaining chapters."

Stalking the Wild Sweetgrass: Domestication and Horticulture of the Grass Used in African-American Coiled Basketry is concerned with the historical domestication of sweetgrass, the main construction/structural grass used in the three century old African-American tradition of coiled basketry in South Carolina. During the plantation era in southern agriculture, sweetgrass baskets were made for post-harvest processing and storage of rice by enslaved Africans from Lower Cape Fear, North Carolina to northern Florida. Enslaved Africans from the Rice Kingdom in Africa were prized for the basketry and rice agronomic skills and were specially sought by slavery traders. Today, this ancient craft still thrives in the community of Mt. Pleasant, South Carolina. Authored by one of the most renowned experts in the field and filled with illuminating color photographs, this volume provides knowledge of the horticulture of an extremely important wild plant and an example of the perils of plant- and people-based research and experimentation. As one of the few authoritative texts on the subject, Stalking the Wild Sweetgrass: Domestication and Horticulture of the Grass Used in African-American Coiled Basketry is a resourceful volume on wild sweetgrass, suitable for researchers and students alike.

The mechanisms underlying endurance and adaptation to environmental stress factors in plants have long been the focus of intense research. Plants overcome environmental stresses by development of tolerance, resistance or avoidance mechanisms, adjusting to a gradual change in its environment which allows them to maintain performance across a range of adverse environmental conditions. Plant Acclimation to Environmental Stress presents the latest ideas and trends on induced acclimation of plants to environmental stresses under changing environment. Written by experts around the globe, this volume adds new dimensions in the field of plant acclimation to abiotic stress factors. Comprehensive and lavishly illustrated, Plant Acclimation to Environmental Stress is a state-of-the-art guide suited for scholars and researchers working in the field of crop improvement, genetic engineering and abiotic stress tolerance.

Due to their sessile lifestyle, plants need to efficiently adapt to changing environmental conditions during their life cycle. Nutrient acquisition from the soil has to be able to adapt to considerable fluctuations in concentrations to ensure adequate distribution between tissues, cells and organelles. The storage and retrieval of nutrients, metabolites or toxic substances in vacuoles plays an important part in cellular homeostasis in plants. The long-range transport and maintenance of turgor is critically dependent on the availability of water and rate of evaporation, while at the same time photosynthetic products have to be transported to all plant parts. As a result plants contain a large number of ATP-dependent pumps and secondary transporters that, in order to adapt to the changing environment, need to be regulated by a complex network of sensing and signaling mechanisms. Plants share many basic elements of signal transduction with animals, but also contain plant-specific signaling molecules and mechanisms. In this volume, the role of transporters and pumps in the regulation of movement, long-range transport and compartmentalization of water, solutes, nutrients and classical signaling molecules is highlighted, and the function, regulation and membrane-transporter interaction and their roles in plant signaling controlling plant physiology and development are discussed.

In plant cells, the plasma membrane is a highly elaborated structure that functions as the point of exchange with adjoining cells, cell walls and the external environment. Transactions at the plasma membrane include uptake of water and essential mineral nutrients, gas exchange, movement of metabolites, transport and perception of signaling molecules, and initial responses to external biota. Selective transporters control the rates and direction of small molecule movement across the membrane barrier and manipulate the turgor that maintains plant form and drives plant cell expansion. The plasma membrane provides an environment in which molecular and macromolecular interactions are enhanced by the clustering of proteins in oligimeric complexes for more efficient retention of biosynthetic intermediates, and by the anchoring of protein complexes to promote regulatory interactions. The coupling of signal perception at the membrane surface with intracellular second messengers also involves transduction across the plasma membrane. Finally, the generation and ordering of the external cell walls involves processes mediated at the plant cell surface by the plasma membrane. This volume is divided into three sections. The first section describes the basic mechanisms that regulate all plasma membrane functions. The second describes plasma membrane transport activity. The final section of the book describes signaling interactions at the plasma membrane. These topics are given a unique treatment in this volume, as the discussions are restricted to the plasma membrane itself as much as possible. A more complete knowledge of the plasma membrane's structure and function is essential to current efforts to increase the sustainability of agricultural production of food, fiber, and fuel crops.

Over recent years, progress in micropropagation has not been as rapid as many expected and, even now, relatively few crops are produced commercially. One reason for this is that the biology of material growing in vitro has been insufficiently understood for modifications to standard methods to be made based on sound physiological principles. However, during the past decade, tissue culture companies and others have invested considerable effort to reduce the empirical nature of the production process. The idea of the conference `Physiology, Growth and Development of Plants and Cells in Culture' (Lancaster, 1992) was to introduce specialists in different areas of plant physiology to micropropagators, with the express aims of disseminating as wide a range of information to as large a number of participants as possible, and beginning new discussions on the constraints and potentials affecting the development of in vitro plant production methods. This book is based on presentations from the conference and has been divided into two main sections, dealing with either aspects of the in vitro environment -- light, nutrients, water, gas -- or with applied aspects of the culture process -- morphogenesis, acclimation, rejuvenation, contamination.

The double helix architecture of DNA was elucidated in 1953. Twenty years later, in 1973, the discovery of restriction enzymes helped to create recombi nant DNA molecules in vitro. The implications of these powerful and novel methods of molecular biology, and their potential in the genetic manipulation and improvement of microbes, plants and animals, became increasingly evi dent, and led to the birth of modern biotechnology. The first transgenic plants in which a bacterial gene had been stably integrated were produced in 1983, and by 1993 transgenic plants had been produced in all major crop species, including the cereals and the legumes. These remarkable achieve ments have resulted in the production of crops that are resistant to potent but environmentally safe herbicides, or to viral pathogens and insect pests. In other instances genes have been introduced that delay fruit ripening, or increase starch content, or cause male sterility. Most of these manipulations are based on the introduction of a single gene - generally of bacterial origi- that regulates an important monogenic trait, into the crop of choice. Many of the engineered crops are now under field trials and are expected to be commercially produced within the next few years. The early successes in plant biotechnology led to the realization that further molecular improvement of plants will require a thorough understanding of the molecular basis of plant development, and the identification and charac terization of genes that regulate agronomically important multi genic traits.

It is very clear nowadays that plants offer several opportunities for basic studies, e.g. on development and embryogenesis, and that the fundamental principles laid open contribute to the development of new tools for plant breeding. Within the scope of the present publication, the editors have had to make a difficult choice from the many important subjects that have contributed to the remarkable progress of our molecular biological understanding of complex biological problems. This has resulted in review papers showing the present state of the art in genetic engineering, gene expression and its manipulation, microbe and insect interactions with plants, transposable elements and gene tagging, plant and organ development, the function and structure of the genome chloroplasts, and lipid biosynthesis. All papers have been written in such a way that they are also useful for non-experts interested in a particular field, as well as for students following courses in plant molecular biology. Besides presenting the state of the art, each paper gives some historical background to the developments in the field as well as perspectives for further basic research and applications. Because of the latter, scientists and students engaged in plant breeding will also profit from this publication.

Optical screening of excessive and potentially harmful solar radiation is an important photoprotective mechanism, though it has received much less attention in comparison with other systems preventing photooxidative damage to photoautotrophic organisms. This photoprotection in the form of screening appears to be especially important for juvenile and senescing plants as well as under environmental stresses-i.e. in situations where the efficiency of enzymatic ROS elimination, DNA repair and other 'classical' photoprotective systems could be impaired. This book represents an attempt to develop an integral view of optical screening-based photoprotection in microalgae and higher plants. Towards this end, the key groups of pigments involved in the screening of ultraviolet and visible components of solar radiation in microalgae and higher plants, and the patterns of their accumulation and distribution within plant cells and tissues, are described. Special attention is paid to the manifestations of screening pigment accumulation in the optical spectra of plants. It is also demonstrated that understanding these effects and their relationships to screening pigments' makeup and spectroscopy in plants provides valuable insights into the state of plants' long-term photoacclimation, as well as ample opportunities for the non-destructive quantification of screening pigments and the assessment of the efficiency of photoprotection providing by these pigments in situ.

This book will shed light on the effect of salt stress on plants development, proteomics, genomics, genetic engineering, and plant adaptations, among other topics. Understanding the molecular basis will be helpful in developing selection strategies for improving salinity tolerance. The book will cover around 25 chapters with contributors from all over the world.

The molecular mechanisms which determine whether the cells of a multicellular organism will live or commit suicide have become a popular field of research in biology during the last decade. Cell death research in the plant field has also been expanding rapidly in the past 5 years. This special volume of Plant Molecular Biology seeks to bring together examples of a diverse array of experimental approaches in a single volume. From the differentiation of tracheary elements in vascular plants to the more specialized cell death model of the aleurone in cereals, this volume will bring the reader up-to-date with the characterization of different plant model systems that are currently being studied. This endeavor should complement general overviews of plant cell death mechanisms that have been published elsewhere by providing more detailed information on various aspects of this field to interested graduate students and more senior biologists alike.

which individuals are heterozygous (H). A review by Selander (1976] comparing these param eters in various populations has been followed by many other studies. In the present volume, J. B. Mitton has used H to evaluate the importance of heterozygosity in natural populations. The degree of polymorphism expressed by P, has been used in several contributions to approach various problems of population genetics. particularly breeding structure and mating systems by Hamrick, Barrett and Shore, Brown, Burdon and Jarosz. as well as Soltis and Soltis, and Wyatt. Stoneburner. and Odrzykoski. New knowledge derived from these investigations has strengthened a point of view already stressed by Darwin: evolution takes place in a complex environment, that can be constantly changing over long periods of time. or can alternate between long periods of relative stability and cycles of rapid change. The most successful plant species become adjusted to these vagaries in several ways, including shifts in heterozygosity. polymorphism and mating systems. The strength of isozyme ana ysis for testing hypotheses is well illustrated by the contribution of the Soltises, who have shown clearly that a previously held hypothesis, predicting self fertilization fortified by polyploid genetic segregations in ferns, must be rejected."

This volume contains selected papers presented at the First Balkan Botanical Congress. The articles refer to all groups of plants and to all scientific disciplines in plant sciences and cover several major themes of current interest to botanists: taxonomy, geobotany and evolution: flora, vegetation, geographical distribution, pollen morphology and deposition, biodiversity, conservation, phytosociology biochemistry, metabolism and bioenergetics: secondary metabolites, enzymes, membrane transport, virus infection ecology and ecophysiology: metal accumulation and tolerance, toxicity and pollution, bio-monitoring systems, dynamics of vegetation communities, leaf structure and ecological types, UV-B and ozone radiation, pesticides and herbicides, ecological evaluation, management and protection of ecosystems, adaptation, photosynthesis structure and its dynamics: organization and molecular characterization of biomembranes and different cell structures and organelles, ultrastructure, anatomy, biosynthesis and localization of different cell compounds genetics, plant breeding and biotechnology: gene transfer, genetic engineering, genetic sterility and diversity, biodiversity and conservation, in vitro regeneration, micropropagation, genotype-environment interaction growth, development and differentiation: differentiation of cell structures, reproductive biology, photoreceptors, crop simulation model, regulators and plant morphogenesis, plant growth patterns, somatic-embryogenesis and organogenesis.

The International Society of Root Research sponsored the Symposium "Root Demographics and Their Efficiencies in Sustainable Agriculture, GrassLands and Forest Ecosystems," July 14-18, 1996, at the Madren Conference Center, Clemson University, Clemson, South Carolina, USA. The conference was a continuation of a series of international symposiums on root research held every three to four years. Symposiums have also been held twice in Vienna, Austria, and once in Uppsala, Sweden, and Almaty, Kazahkstan prior to the meeting at Clemson University. The sponsoring society has made a particular effort in these symposia to include root scientists from the former Soviet Union because of the importance of exchanging information on a worldwide basis. This symposium continued and promoted that effort by providing travel grants to several scientists from that region; however, funds for that purpose were limited. Therefore, in compiling these proceedings, a number of papers from scientists from the former Soviet Union and former Warsaw Pack countries have been included even though the scientists were not actually present for the SymPOSIum.

Achievements today in plant biotechnology have already surpassed all previous expectations. Plant biotechnology, integrated with classical breeding, is now on the verge of creating the evergreen revolution' to solve the world's envisaged tripled demand for food, agricultural commodities and natural products. New biotechnologies are being continuously adapted to agricultural practices, opening new vistas for plant utilization. Plant biotechnology is changing the plant scene in three major areas: (1) growth and development control (vegetative, generative and propagation), (2) protecting plants against the ever-increasing threats of abiotic and biotic stress, (3) expanding the horizons by producing specialty foods, biochemicals and pharmaceuticals. The potential for improving plant and animal productivity and their proper use in agriculture relies largely on newly-developed DNA biotechnology and molecular markers. These techniques enable the selection of successful genotypes, better isolation and cloning of favorable traits, and the creating of transgenic organisms of importance to agriculture. These areas were extensively discussed at the 9th international congress of the International Association of Plant Tissue Culture and Biotechnology, Plant Biotechnology and In Vitro Biology in the 21st Century', which was held in Jerusalem in June 1998. The present book of proceedings contains the variety of scientific achievements and techniques that were presented: Basic and Applied Aspects of Growth, Development and Differentiation; Genetic Manipulations: Transformation and Gene Expression, Hybridization, Haploidization and Mutagenesis; Genetic Stability and Instability, Selection and Variability; Regulation of Primary and Secondary Metabolism; Model Systems: Cell Cycle, Transport and Signal Transduction; Biotechnology for Plant Protection: Abiotic and Biotic Stress; Biotechnology for Crop Improvement: Yield, Quality and Production of Valuable Substances; Novel Micropropagation Methods; New Markets and Commercial Applications; Intellectual Property Rights.

The Fifth International Symposium on Nitrogen Fixation with Non-legumes was held in Florence (Italy) on 10-14 September, 1990. Earlier Symposia of this series were held in Piracicaba (Brazil), Banf Alberta (Canada), Helsinki (Finland) and Rio De Janeiro (Brazil). The Symposium's main objectives were to bring together scientists working in many different fields of nitrogen fixation, to stimulate discussion on this important process and to have an appraisal of the most recent studies concerning nitrogen fixation with non-legumes. The Symposium was attended by 230 scientists from 32 different countries. This volume collects the contributions of 65 lectures and 87 posters, which are an up-to-date account of the state of knowledge on biological nitrogen fixation with non-legumes. The book provides a valuable reference source not only for specialists in nitrogen fixation, but also for researchers working on related aspects of agronomy, biochemistry, genetics, microbiology, molecular biology and plant physiology. It is with great pleasure that we aknowledge the contributions of the authors in assuring the prompt pubblication of this volume. We would also like to express our thanks to Kluwer Academic Publishers B.V. for the publication of these Proceedings. M. Polsinelli R. Materassi M. Vincenzini ORGANIZING COMMITTEE President M. Polsinelli M. Vincenzini Secretary F. Favilli Treasurer E. Galli E. Gallori L. Giovannetti R. Materassi M.P. Nuti M.R. Tredici SCIENTIFIC COMMITTEE M. Bazzicalupo Florence, Italy H. Bothe Cologne, West Germany R.H. Burris Madison, U.S.A.