The first edition of this book was the first to provide an integrated description of sap ascension from an anatomical and functional point of view. The second edition opens with the three-dimensional aspects of wood anatomy. The cohesion-tension theory and new evidence are introduced in response to recent controversies over the mechanism of sap ascent in plants. The physiology, anatomy and biophysics of xylem dysfunction are discussed and new insights into hydraulic architecture are reviewed with special emphasis on physiological limits on maximum transpiration and how hydraulic architecture limits gas exchange, carbon gain and growth of plants. The text concludes with a description of xylem failure and pathology. The book highlights fascinating areas of current research with the aim to stimulate more work in the future.

This volume presents the proceedings of the Second International Sym posium on Genetic Aspects ofPlant Minerai Nutrition, held in Madison, Wisconsin in 1985. The mechanisms by which plants acquire, transport and utilize essential minerai nutrients are highly complex. The means by which plants either exclude or tolerate ions of metals toxic to plants are equally complex. The first symposium attempted to convene research scientists con cerned with minerai nutrition for the purpose of exploring the kinds of minerai nutrition phenomena identified as being under genetic contro!. The first symposium also placed much emphasis on research to which genetic intervention might be applied. At the second symposium more papers were presented on genetic and breeding research, a long-term objective of the first symposium. The second symposium also included biotic interactions under genetic con trol that either enhanced or impeded ion uptake, e.g. mycorrhizae and nitrogen fixing bacteria. This continuing dialogue is essential for a research area the complexity of which is due to its interdisciplinary nature.

The West African Sahel is the transition zone between the Saharadesert in the north of Africa and the more humid Sudanian zones in the south. Although diverse in many ways, the Sahelian countries have the common problem of a fragile agricultural sector. This predicament is mainly caused by low inherent soil fertility, limited and unpredictable rainfall, frequent droughts, and wind erosion that accelerates soil degradation and desertification, compounded by To assure food production in the future, means rapidly growing populations. of declining soil fertility and increasing must be found to offset the trends soil degradation through wind erosion. This is a challenge for agricultural research. Since 1985, the Special Research Program 308 'Adapted Farming in West Africa' at the UniversityofHohenheimin collaboration with the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) in Niger, has pursued the developmentof agricultural innovations for smallholder farmers in one of the most ecologically fragile regions of the world. The prevention of soil degradation, the restoration and maintenance of soil fertility, and the increase of land and labor productivity are key objectives of this multidisci plinary research program. From the beginning, a major focus of research has been wind erosion.

Since the first volume on Biophysical Techniques in Photosynthesis Research, published in 1996, new experimental techniques and methods have been devised at a rapid pace. The present book is a sequel which complements the first volume by providing a comprehensive overview of the most important new techniques developed over the past ten years, especially those that are relevant for research on the mechanism and fundamental aspects of photosynthesis.

The contributions are written by leading scientists in their field. The book is divided into 5 sections on Imaging, Structure, Optical and laser spectroscopy, Magnetic resonance and on Theory, respectively. Each chapter describes the basic concepts of the technique, practical applications and some of the scientific results. Possibilities and limitations from a technical as well as a scientific point of view are addressed, allowing the reader not only to recognize the potential of a particular method for his/her own quest, but to assess the resources that are required for implementation.

The application of imaging techniques in plant and agricultural sciences had previously been confined to images obtained through remote sensing techniques. Technological advancements now allow image analysis for the nondestructive and objective evaluation of biological objects. This has opened a new window in the field of plant science. Plant Image Analysis: Fundamentals and Applications introduces the basic concepts of image analysis and discusses various techniques in plant imaging, their applications, and future potential. Several types of imaging techniques are discussed including RGB, hyperspectral, thermal, PRI, chlorophyll fluorescence, ROS, and chromosome imaging. The book also covers the use of these techniques in assessing plant growth, early detection of disease and stress, fruit crop yield, plant chromosome analysis, plant phenotyping, and nutrient status both in vivo and in vitro. The book is an authoritative guide for researchers and those teaching in the fields of stress physiology, precision agriculture, agricultural biotechnology, and cell and developmental biology. Graduate students and professionals using machine vision in plant science will also benefit from this comprehensive resource.

Sixty years ago at the Waite Agricultural Research Institute, G. Samuel, a plant pathologist, and C. S. Piper, a chemist, published their conclusion that the cause of roadside take-all, a disease of oats, was manganese deficiency. This report, together with the concurrent and independent studies of W. M. Carne in Western Australia were the first records of manganese deficiency in Australia and came only six years after McHargue's paper which is generally accepted as the final proof of the essentiality of this element. There must have been a few doubts for some people at the time, however, as the CAB publication, 'The Minor Elements of the Soil' (1940) expressed the view that further evidence to this effect was provided by Samuel and Piper. Their historic contributions are recognised by the International Symposium on Manganese in Soils and Plants as it meets on the site of their early labours to celebrate the 60th anniversary. This year Australians also acknowledge 200 years of European settlement in this country and so the Symposium is both a Bicentennial and a diamond jubilee event which recognises the impact of trace elements on agricultural development in Australia. In a broader sense, a symposium such as this celebrates, as it reviews, the efforts of all who over the ages have contributed to our knowledge of manganese in soils and plants.

With one volume published each year, this series keeps scientists and students current with the latest developments and results in all areas of the plant sciences. This present volume includes insightful reviews covering genetics, cell biology, physiology, comparative morphology, systematics, ecology, and vegetation science.

Lipids in Photosynthesis provides readers with a comprehensive view of the structure, function and genetics of lipids in plants, algae and bacteria, with special emphasis on the photosynthetic apparatus in thylakoid membranes. This volume includes the historical background of the field, as well as a full review of our current understanding of the structure and molecular organization of lipids and their role in the functions of photosynthetic membranes. The physical properties of membrane lipids in thylakoid membranes and their relationship to photosynthesis are also discussed. Other topics include the biosynthesis of glycerolipids and triglycerides; reconstitution of photosynthetic structures and activities with lipids; lipid-protein interactions in the import of proteins into chloroplasts; the development of thylakoid membranes as it relates to lipids; genetic engineering of the unsaturation of membrane glycerolipids, with a focus on the ability of the photosynthetic machinery to tolerate temperature stress; and the involvement of chloroplast lipids in the reactions of plants upon exposure to stress. This book is intended for a wide audience and should be of interest to advanced undergraduate and graduate students and to researchers active in the field, as well as to those scientists whose fields of specialization include the biochemistry, physiology, molecular biology, biophysics and biotechnology of membranes.

The primary aim of Wood Structure and Environment is to reveal the hidden ecological richness in stems and roots from trees, shrubs and herbs. The detailed, lucid text will inspire researchers to consider the anatomic microcosm of wood plants and use it as a retrospective source of information, solving problems related to ecophysiology, competition, site conditions, population biology, earth science, wood quality and even human history.

Plant neurobiology is a newly emerging field of plant sciences. It covers signalling and communication at all levels of biological organization - from molecules up to ecological communities. In this book, plants are presented as intelligent and social organisms with complex forms of communication and information processing.

Authors from diverse backgrounds such as molecular and cellular biology, electrophysiology, as well as ecology treat the most important aspects of plant communication, including the plant immune system, abilities of plants to recognize self, signal transduction, receptors, plant neurotransmitters and plant neurophysiology. Further, plants are able to recognize the identity of herbivores and organize the defence responses accordingly. The similarities in animal and plant neuronal/immune systems are discussed too. All these hidden aspects of plant life and behaviour will stimulate further intense investigations in order to understand the communicative plants in their whole complexity.

This book reviews all aspects of boron research in recent years and is based on the Third International Symposium on all Aspects of Plant and Animal Boron Nutrition. This includes B sorption mechanisms in soils, deficiency and toxicity of B, B fertilizer application and basic research on the physiology and molecular biology of plant B nutrition, and nutritional function of B in animals and humans.

The fermentation of sugar by cell-free yeast extracts was demonstrated more than a century ago by E. Buchner (Nobel Prize 1907). Buchner's observations put an end to previous animistic theories regarding cellular life. It became clear that metabolism and all cellular functions should be accessible to explication in chemical terms. Equally important for an understanding of living systems was the concept, explained in physical terms, that all living things could be cons- ered as energy converters [E. Schroedinger (Nobel Prize 1933)] which generate complexity at the expense of an increase in entropy in their environment. Bioenergetics was established as an essential branch of the biochemical sciences by the investigations into the chemistry of photosynthesis in i- lated plant organelles [O. Warburg (Nobel Prize 1931)] and by the discovery that mitochondria were the morphological equivalent that catalyzed cellular respiration. The ?eld of bioenergetics also encompasses a large variety of ad- tional processes such as the molecular mechanisms of muscle contraction, the structure and driving mechanisms of microbial ?agellar motors, the energetics of solute transport, the extrusion of macromolecules across membranes, the transformation of quanta of light into visual information and the maintenance of complex synaptic communications. There are many other examples which, in most cases, may perform secondary energy transformations, utilizing - ergy stored either in the cellular ATP pool or in electrochemical membrane potentials.

The Second International Congress on Photosynthesis Research took place in Stresa, Italy during June 24-29, 1971; two centuries after the discovery of Photosynthesis by Joseph Priestley in 1971. This important anniversary was celebrated at the Congress by a learned account of Priestley's life and fundamental discoveries given by Professor Robin HILL, F. R. S. Professor HILL's lecture opens the first of the three volumes which contains the contributions presented at the Congress. The manuscripts have been distributed into three volumes. Volume I con tains contributions in the areas of primary reactions and electron transport; Volume II ion transport and photophosphorylation, and Volume III carbon assimilation, regulatory phenomena, developmental aspects, and from the two special sessions of the Congress devoted to evolution and photorespiration. It is realized that this division is necessarily somewhat arbitrary since many contributions relate to more than one of the above mentioned titles. However, the large number of contributions (over 3000 typed pages) made it impossible to publish the proceedings in less than three volumes. The printing of these volumes and the organization of the Congress were made possible by a contribution from the Consigio Nazionale delle Ricerche of Italy. The generous support of the Istituto Lombardo Acca demia di Scienze e Lettere to the publication of these proceedings is gratefully acknowledged. The editors wish to express their appreciation to all the scientists who contributed the results of the investigations, for their coopera tion; and to Drs."

Since the beginning of agricultural production, there has been a continuous effort to grow more and better quality food to feed ever increasing popula tions. Both improved cultural practices and improved crop plants have al lowed us to divert more human resources to non-agricultural activities while still increasing agricultural production. Malthusian population predictions continue to alarm agricultural researchers, especially plant breeders, to seek new technologies that will continue to allow us to produce more and better food by fewer people on less land. Both improvement of existing cultivars and development of new high-yielding cultivars are common goals for breeders of all crops. In vitro haploid production is among the new technologies that show great promise toward the goal of increasing crop yields by making similar germplasm available for many crops that was used to implement one of the greatest plant breeding success stories of this century, i. e., the development of hybrid maize by crosses of inbred lines. One of the main applications of anther culture has been to produce diploid homozygous pure lines in a single generation, thus saving many generations of backcrossing to reach homozygosity by traditional means or in crops where self-pollination is not possible. Because doubled haploids are equivalent to inbred lines, their value has been appreciated by plant breeders for decades. The search for natural haploids and methods to induce them has been ongoing since the beginning of the 20th century."

Flowering plants dominate much of the Earth's surface and yet, as sessile organisms, they must constantly resist attack by numerous voracious herbivores. Survival in the face of an abundance of insect predators relies on sophisticated resistance systems allowing plants to escape from herbivory in time or in space, to confront herbivores directly, or to fight them indirectly by remarkable collaborations with other species. Until recently, plant resistance was believed to be constitutive, i.e. ever-present and independent from herbivore attack. However, plants were discovered to respond actively to herbivory through the mobilization of specific defenses, and this discovery opened an exciting new field of research.
This book provides a thorough overview of the anatomical, chemical, and developmental features contributing to plant defense, with particular emphasis on plant responses that are induced by wounding or herbivore attack.

Adaptation and evolution of terrestrial plants depend, to a large extent, on their ability to acquire nutrients. This is a modern and integrative treatment of the mechanisms controlling plant nutrient uptake and how plants respond to changes in the environment. The following key topics are covered: soil nutrient bioavailability; root responses to variations in nutrient supply; nitrogen fixation; regulation of nutrient uptake by internal plant demand; root characteristics; kinetics of nutrient uptake; root architecture; life span; mycorrhizae; responses to climate change. This integrated view helps us to understand the mechanisms that govern present-day plant communities and is indispensable in models designed to predict the response of plants to a changing climate.

The Conference on Tropical Rainforest Research: Current Issues was organised by the University of Brunei Darussalam and The Royal Geographical Society, London, and held in Bandar Seri Begawan, Brunei Darussalam, over 8 days in April 1993. Over 160 participants from 22 countries attended the Conference, which was opened on the 9th April by the Brunei Darussalam Minister for Home Affairs, Yang Berhormat Pehin Orang Kaya Laila Setia Bakti Diraja Dato Laila Utama Haji Awang Isa bin Datu Perdana Menteri Dato Laila Utama Haji Awang Ibrahim. The conference was initially intended to provide a forum to present the results of the expedition into the lowland mixed dipterocarp forest of the Temburong District of Brunei Darussalam, which had been jointly organised by the University of Brunei Darussalam and The Royal Geographical Society, London. The 15-month expedition, lasting from January 1991 to March 1992, was based at the then newly-completed Kuala Belalong Field Studies Centre, a research and education facility set up by the University with funds provided by the Brunei Government and Brunei-Shell Petroleum Sdn Bhd. The expedition, with over 70 scientists taking part, received financial support from a wide range of sponsors and Corporate Patrons, including Royal Brunei Airlines, the Baring Foundation, Daiwa-Dicam, Greencard Trust, the Hongkong Bank, Morgan Grenfell and Nomura-Nimco. The conference itself was supported by donations from Brunei-Shell Sdn Bhd, Royal Brunei Airlines and Standard Chartered Bank.

We are facing global issues concerning environmental pollution and shortages of food, feed, phytomass (plant biomass) and natural resources, which will become more serious in the forthcoming decades. To solve these issues, immeasurable numbers of various plants and huge amounts of phytomass are required every year for food, feed and for the improvement of amenities, the environment and our quality of life. Increased phytomass is also required as alternative raw material for producing bio-energy, biodegradable plastics and many other plant-originated industrial products. Only by using phytomass as a reproducible energy source and raw material, instead of fossil fuels and atomic power, we can save natural resources and minimize environmental pollution. To increase phytomass globally, we need billions of quality transplants (small plants) to be grown yearly, in the field or in the greenhouse, under various environmental conditions. However, these high quality transplants can be produced only under carefully controlled, rather than variable environment al conditions. Recent research has shown that the closed transplant production system requires considerably small amounts of electricity, water, fertilizer, CO) and pesticide to produce value-added transplants as scheduled with minimum release of environmental pollutants and minimum loss of transplants. The closed or closed-type transplant production system is defined as a transplant production system covered with opaque walls with minimized or controlled ventilation rates, using artificial lighting. With this system, photoperiod, light intensity and quality, air temperature, humidity, CO) concentration and air current speed can be controlled as desired.

This widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published.

The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures.

Photosynthesis and the Environment examines how photosynthesis may be influenced by environmental changes. Structural and functional aspects of the photosynthetic apparatus are examined in the context of responses to environmental stimuli; particular attention being given to the processing of light energy by thylakoids, metabolic regulation, gas exchange and source-sink relations. The roles of developmental and genetic responses in determining photosynthetic performance are also considered. The complexity of the responses to environmental change is demonstrated by detailed analyses of the effects of specific environmental variables (light, temperature, water, CO2, ozone and UV-B) on photosynthetic performance. Where appropriate attention is given to recent developments in the techniques used for studying photosynthetic activities.

The book is intended for advanced undergraduate and graduate students and a wide range of scientists with research interests in environmental effects on photosynthesis and plant productivity.

Genetic variability is an important parameter for plant breeders in any con ventional crop improvement programme. Very often the desired variation is un available in the right combination, or simply does not exist at all. However, plant breeders have successfully recombined the desired genes from cultivated crop gerrnplasm and related wild species by sexual hybridization, and have been able to develop new cultivars with desirable agronomie traits, such as high yield, disease, pest, and drought resistance. So far, conventional breeding methods have managed to feed the world's ever-growing population. Continued population growth, no further scope of expanding arable land, soil degradation, environ mental pollution and global warrning are causes of concern to plant biologists and planners. Plant breeders are under continuous pressure to improve and develop new cultivars for sustainable food production. However, it takes several years to develop a new cultivar. Therefore, they have to look for new technologies, which could be combined with conventional methods to create more genetic variability, and reduce the time in developing new cultivars, with early-maturity, and improved yield. The first report on induced mutation of a gene by HJ. Muller in 1927 was a major mi1estone in enhancing variation, and also indicated the potential applica tions of mutagenesis in plant improvement. Radiation sources, such as X-rays, gamma rays and fast neutrons, and chemical mutagens (e. g., ethyl methane sulphonate) have been widely used to induce mutations."

Combining an ecosystems approach with new insights at the molecular and biochemical level, this book presents the latest findings on how plants respond, physiologically, to sulfur in their environment. It explores key areas such as biotic and abiotic interactions, adaptations to fluctuating supply, and sulfur s role in plant metabolic networks to assess the functions and implications of this essential plant nutrient in a range of natural, semi-natural and anthropogenic environments.

Leaders in the field, Hawkesford and de Kok, draw together an international group of experts in plant sulfur nutrition, to collate and integrate new information from molecular biology, ecology and physiology. The result is an important new reference on the relationships between plants and sulfur at all levels including contributions arising from recent omic approaches. With implications for issues such as agriculture, forestry, water management and ecosystem restoration, this book is relevant to a wide audience, from graduate level students and researchers, to policy makers and practitioners."

Glutathione is a thiol-containing tripeptide, which appears to be present in nearly all living organisms and which is involved in many important metabolic and physiological processes. The present volume focuses on the biological significance of glutathione in plants. The biochemistry and the metabolism of glutathione are reviewed, and its role in sulphur and selenium metabolism in plants is discussed. The significance of glutathione and of glutathione-related enzymes in the adaptation to natural stress, heavy metals, xenobiotics, air pollution, and in plant-pathogen and plant-animal interactions are evaluated. The main aim of this second volume in the series Plant Ecophysiology is to raise the interest of advanced students and junior researchers in the role of glutathione in plants and to supply basic and comprehensive information for scientists already working on related topics.

In the last two decades technological advances in isotope ratio mass spectrometry have been very rapid, opening up new possibilities for analysis of biological and environmental materials. The new instrumentation has facilitated faster analysis of samples via automated sample preparation and multi-isotope analysis of single samples, resulting in considerable cost savings, and enabling access to isotope analysis for many more researchers. These changes are reflected in the rapidly growing international literature on stable isotopes. While there have been some excellent books and review papers aimed at interpreting isotope signals in biology and environmental science, there have been fewer attempts to provide practical tools for researchers making forays into this exciting new arena. This book aims to address this inadequacy by providing a set of practical guidelines for the application of a range of novel and well proven stable isotope techniques to the fields of plant physiological ecology, agriculture, marine ecology and palaeoecology. The book is the outcome of a weeklong workshop held under the auspices of the Cooperative Research Centre for Legumes in Mediterranean Agriculture (CLIMA 1992 - 2000) at The University of Western Australia and the CSIRO Floreat Laboratories, Perth, Western Australia, in February 1999. The workshop was designed to provide practical tools and experiences for researchers and students concerned with how one goes about using stable isotopes in field investigations.

Plant growth is of great economical and intellectual interest. Plants are the basis of our living environment, the production of our food and a myriad of plant-based natural products. Plant bio-mass is also becoming an important renewable energy resource. Agricultural plant cultivation and breeding programs have altered plant productivity and yield parameters extensively, yet the principles and underlying mechanisms are not well understood. At the cellular level, growth is the result of only two processes, cell division and cell expansion, but these two processes are controlled by intertwined signaling cascades and regulatory mechanisms forming complex regulatory networks. Ultimately this network is what plant scientists are trying to unravel. The sequencing of model and agronomically important plant genomes allows complete insight into the molecular components involved in each process. Methods to quantify the molecular changes, image growth processes and reconstruct growth regulatory networks are rapidly developing. This knowledge should help to elucidate key regulators and to design methods to engineer plant architecture and growth parameters for future human needs. This volume gives a comprehensive overview of what is known about plant growth regulation and growth restraints due to environmental conditions and should allow readers at all levels an entry into this exiting field of research.