This book covers the emerging topic of DNA nanotechnology and DNA supramolecular chemistry in its broader sense. By taking DNA out of its biological role, this biomolecule has become a very versatile building block in materials chemistry, supramolecular chemistry and bio-nanotechnology. Many novel structures have been realized in the past decade, which are now being used to create molecular machines, drug delivery systems, diagnosis platforms or potential electronic devices. The book combines many aspects of DNA nanotechnology, including formation of functional structures based on covalent and non-covalent systems, DNA origami, DNA based switches, DNA machines, and alternative structures and templates. This broad coverage is very appealing since it combines both the synthesis of modified DNA as well as designer concepts to successfully plan and make DNA nanostructures. Contributing authors have provided first a general introduction for the non-specialist reader, followed by a more in-depth analysis and presentation of their topic. In this way the book is attractive and useful for both the non-specialist who would like to have an overview of the topic, as well as the specialist reader who requires more information and inspiration to foster their own research.

How tiny variations in our personal DNA can determine how we look, how we behave, how we get sick, and how we get well. News stories report almost daily on the remarkable progress scientists are making in unraveling the genetic basis of disease and behavior. Meanwhile, new technologies are rapidly reducing the cost of reading someone's personal DNA (all six billion letters of it). Within the next ten years, hospitals may present parents with their newborn's complete DNA code along with her footprints and APGAR score. In Genetic Twists of Fate, distinguished geneticists Stanley Fields and Mark Johnston help us make sense of the genetic revolution that is upon us. Fields and Johnston tell real life stories that hinge on the inheritance of one tiny change rather than another in an individual's DNA: a mother wrongly accused of poisoning her young son when the true killer was a genetic disorder; the screen siren who could no longer remember her lines because of Alzheimer's disease; and the president who was treated with rat poison to prevent another heart attack. In an engaging and accessible style, Fields and Johnston explain what our personal DNA code is, how a few differences in its long list of DNA letters makes each of us unique, and how that code influences our appearance, our behavior, and our risk for such common diseases as diabetes or cancer.

In 2001 the Human Genome Project announced that it had successfully mapped the entire genetic content of human DNA. Scientists, politicians, theologians, and pundits speculated about what would follow, conjuring everything from nightmare scenarios of state-controlled eugenics to the hope of engineering disease-resistant newborns. As with debates surrounding stem-cell research, the seemingly endless possibilities of genetic engineering will continue to influence public opinion and policy into the foreseeable future.

Beyond Biotechnology: The Barren Promise of Genetic Engineering distinguishes between the hype and reality of this technology and explains the nuanced and delicate relationship between science and nature. Authors Craig Holdrege and Steve Talbott evaluate the current state of genetic science and examine its potential applications, particularly in agriculture and medicine, as well as the possible dangers. The authors show how the popular view of genetics does not include an understanding of the ways in which genes actually work together in organisms.

Simplistic and reductionist views of genes lead to unrealistic expectations and, ultimately, disappointment in the results that genetic engineering actually delivers. The authors explore new developments in genetics, from the discovery of "non-Darwinian" adaptative mutations in bacteria to evidence that suggests that organisms are far more than mere collections of genetically driven mechanisms. While examining these issues, the authors also answer vital questions that get to the essence of genetic interaction with human biology: Does DNA "manage" an organism any more than the organism manages its DNA? Should genetically engineered products be labeled as such? Do the methods of the genetic engineer resemble the centuries-old practices of animal husbandry?

Written for lay readers, Beyond Biotechnology is an accessible introduction to the complicated issues of genetic engineering and its potential applications. In the unexplored space between nature and laboratory, a new science is waiting to emerge. Technology-based social and environmental solutions will remain tenuous and at risk of reversal as long as our culture is alienated from the plants and animals on which all life depends.

This book provides an overview of different ethical views on animal experimentation. Special attention is given to the production and experimental use of genetically modified animals. It proposes a middle course between those positions that are very critical and those very positive. This middle course implies that animal experiments originating in vital human research interests are commonly justified, provided that animal welfare is taken seriously. Some animal experiments are not acceptable, since the expected human benefit is too low and the animal suffering too severe. This position is supported by an argument from species care according to which we have special obligations to our children and other humans due to special relations. The book tries to bridge the gap between animal ethics and animal welfare science by discussing various conceptions of animal welfare: function-centered, feeling-based, and those focusing on natural living. The theoretical starting-point is "imaginative casuistry." This approach stresses the role of moral imagination and metaphor in ethical deliberation, accepts a plurality of values, and recognizes the importance of case-by-case balancing. In the discussion of genetically modified animals, both intrinsic ethical concerns and animal welfare concerns are addressed.

A research biologist tells the clear-eyed story of modern plant genetics, the human manipulation of biotechnology, and the reality of genetically engineered plants worldwide. Genetically engineered plant products line the shelves of our grocery stores, but we don't know which ones they are because no label identifies them. Should we be concerned? It is true that biotech companies are saying that engineered corn and canola are safe, but are they telling the truth? In High Tech Harvest, Paul Lurquin answers these questions and more, believing that the public has a right to know and understand how its food is manipulated at the most basic level, that of the DNA itself. With the goal to inform, and a mission to reinforce the importance of the scientific method, Paul Lurquin writes a comprehensive and user-friendly description of the scientific origins, the development, and the applications of genetically modified plants throughout the world today. Lurquin argues that only with an understanding of the basic science can people make informed and reasonable decisions about genetically modified foods.

The book addresses some fundamental and profound questions such as: Are GM foods safe to eat? What do consumers think about GM foods and, alternatively, organic produce? What are the real risks of genetic pollution? And is it appropriate to delete a supposed gene for sadness? 'Recoding Nature' challenges the assumptions of those preparing the world for a 'recoded' DNA future. Recoding Nature is at the cutting edge of critical reflection about the 'biotechnology revolution', the redesign of nature through genetically modified plants, animals and even designer humans. The book addresses some fundamental and profound questions: Are GM foods safe to eat? What do consumers think about GM foods and, alternatively, organic produce? What are the real risks of genetic pollution? Is it appropriate to delete a supposed gene for sadness? Where did the idea of the DNA code come from, and how is it shaping thought for a genetics future? Why has commercial release of GM canola been approved when all canola-growing States have declared moratoriums? Does the biomedical approach really offer the way forward in health care? Are there genes for crime, or is this just an illusion? What about the prospects of corporate bioprospecting among Indigenous peoples? And why have large grass-roots movements in Asia surfaced to contest the notion that GM foods will feed the hungry? In fourteen essays by Australian and New Zealand writers critiquing the new biology, and with a stimulating foreword by Mae-Wan Ho - the UK scientist leading a global attack on genetic engineering as 'bad science' - Recoding Nature challenges the assumptions of those preparing the world for a 'recoded' DNA future.

Few avenues of scientific inquiry raise more thorny ethical questions than the cloning of human beings, a radical way to control our DNA. In August 2001, in conjunction with his decision to permit limited federal funding for stem-cell research, President George W. Bush created the President's Council on Bioethics to address the ethical ramifications of biomedical innovation. Over the past year the Council, whose members comprise an all-star team of leading scientists, doctors, ethicists, lawyers, humanists, and theologians, has discussed and debated the pros and cons of cloning, whether to produce children or to aid in scientific research. This book is its insightful and thought-provoking report.

The questions the Council members confronted do not have easy answers, and they did not seek to hide their differences behind an artificial consensus. Rather, the Council decided to allow each side to make its own best case, so that the American people can think about and debate these questions, which go to the heart of what it means to be a human being. Just as the dawn of the atomic age created ethical dilemmas for the United States, cloning presents us with similar quandaries that we are sure to wrestle with for decades to come.

The Cyborg Experiments analyzes the challenges posed to corporeality by techology. Taking as their starting point the work of the highly influential performance artists Orlan and Stelarc, the essays in this timely and important collection raise a number of questions in relation to new conceptions of embodiment, identity and otherness in the age of new technologies: Has the body become obsolete? Does transgender challenge traditional ideas of agency? Have we always been cyborgs?In addition to highlighting the playful character of digital aesthetics, the contributors investigate ethical issues concerning the ownership of our bodies and the experiments we perform on them. In this way the book explores how humanism, and ideas of the human, have been placed under increasing scrutiny as a result of new developments in science, media and communications.Contributors:John Appleby, Rachel Armstrong, Fred Botting, Julie Clarke, Gary Hall, Chris Hables Gray, Meredith Jones, Orlan, Mark Poster, Jay Prosser, E. A. Scheer, Zod Sofia, Stelarc, Scott Wilson, Joanna Zylinska.

Ever since Dolly, the Scottish lamb, tottered on wobbly legs into our consciousness-followed swiftly by other animals: first, mice; then pigs that may provide human transplants, and even an ordinary house cat-thoughts have flown to the cloning of human beings. Legislators rushed to propose a ban on a technique that remains highly hypothetical, although some independent researchers have announced their determination to pursue the possibilities. Political scientist and well-known expert on reproductive issues, Andrea L. Bonnicksen examines the political reaction to this new-born science and the efforts to construct cloning policy. She also looks at issues that relate to stem cell research, its even newer sibling, and poses a key question:

How does the response to Dolly guide us as we manage innovative reproductive technologies in the future?

Various legislative endeavors and the efforts by the Food and Drug Administration (FDA) to oversee cloning, as well as policy models related to federal funding, individual state laws, and programs abroad, inform Bonnicksen's identification of four types of cloning policy. She analyzes in depth the roles of diverse interest groups as each struggle to become the dominant voice in the decision-making process. With skill and insight, she clears the mists from a complicated topic, and addresses the legal, political, and ethical arguments that are not likely to disappear from the national conversation or debates any time soon.

For this forth edition, the book has been updated thoroughly, with particular emphasis on modulation of chromatin structure by histone modifications/ remodelling complexes and the role of co-activators/ co-repressors. Methods used to analyse gene expression have also been given more attention, with a new section added on methods for examining DNA binding by transcription factors. Additionally, new sections have been added on coupling of transcription factors. Additionally, new sections have been added on coupling of transcription with post-transcriptional process and negatively acting sequence elements, which are of increasing prominence.

Providing the first account of the story behind genetically engineered plants, Paul F. Lurquin covers the controversial birth of the field, its sudden death, phoenixlike reemergence, and ultimate triumph as not only a legitimate field of science but a new tool of multinational corporate interests. In addition, Lurquin looks ahead to the potential impact this revolutionary technology will have on human welfare.

As Lurquin shows, it was the intense competition between international labs that resulted in the creation of the first transgenic plants. Two very different approaches to plant genetic engineering came to fruition at practically the same time, and Lurquin's account demonstrates how cross-fertilization between the two areas was critical to success. The scientists concerned were trying to tackle some very basic scientific problems and did not foresee the way that corporations would apply their methodology. With detailed accounts of the work of individual scientists and teams all over the world, Lurquin pieces together a remarkable account.

Providing the first account of the story behind genetically engineered plants, Paul F. Lurquin covers the controversial birth of the field, its sudden death, phoenixlike reemergence, and ultimate triumph as not only a legitimate field of science but a new tool of multinational corporate interests. In addition, Lurquin looks ahead to the potential impact this revolutionary technology will have on human welfare.

As Lurquin shows, it was the intense competition between international labs that resulted in the creation of the first transgenic plants. Two very different approaches to plant genetic engineering came to fruition at practically the same time, and Lurquin's account demonstrates how cross-fertilization between the two areas was critical to success. The scientists concerned were trying to tackle some very basic scientific problems and did not foresee the way that corporations would apply their methodology. With detailed accounts of the work of individual scientists and teams all over the world, Lurquin pieces together a remarkable account.

The application of computational methods to DNA and protein science is a new and exciting development in biology. Bioinformatics: Sequence and Genome Analysis is a comprehensive introduction to this emerging field of study. The book has many unique and valuable features:
-- It is written for any biologist who wants to understand methods of sequence and structure analysis and how the necessary computer programs work
-- Sequence alignment, structure prediction, phylogenetic and gene prediction, database searching, and genome analysis are clearly explained and amply illustrated
-- Underlying algorithms and assumptions are clearly explained for the non-specialist
-- Examples are presented in simple numerical terms rather than complex formulas and notation
-- Theoretical underpinnings are linked to biological problems and their solutions
-- Extensive tables provide descriptions and Web sources for a broad range of publicly available software
-- An associated Website (www.bioinformaticsonline.org), accessible free of charge by book purchasers, provides links to Internet sources referred to in the text, as well as problem sets for classroom use, and other useful material not included in the text.

Based on the well-established course given at the University of Arizona by the author, David Mount, this book is an ideal foundation for teaching at an undergraduate and graduate level. It is also highly suited for the self-instruction of investigators interested in the application of methods and strategies in functional genomics and for the needs of information specialists working in molecular biology and pharmaceutical laboratories.

Identification and Expression of G Protein-Coupled Receptors edited by Kevin R. Lynch The past decade has seen tremendous advances in the study of G protein-coupled receptors (GPCRs), including the molecular cloning and identification of more than 100 GPCR genes. But while GPCRs serve as targets for more than 300 medicines in the modern pharmacopoeia, the shrinking pool of known ligands and the continuing discovery of orphan GPCR genes have underscored the need for new approaches to ligand identification. Identification and Expression of G Protein-Coupled Receptors addresses this new direction in GPCR biochemistry-offering a definitive laboratory bench manual that emphasizes expression over primary cloning strategies. In a series of expert contributions by well-known researchers, this book provides detailed protocols for various expression systems-from bacteria to mammalian cells-as well as straightforward opinions on the advantages and shortcomings of each approach. Topics covered include:
* Homology screening and the polymerase chain reaction in the cloning of GPCR genes
* Cloning of GPCRs using mammalian cell expression
* GPCR informatics and the orphan problem
* The use of Xenopus laevis oocytes for the study of GPCRs
* Stable expression of GPCRs in mammalian cells
* Heterologous expression in primary cell cultures
* Expression of GPCRs in Escherichia coli
* Large scale expression and purification of GPCRs in mammalian cells
* High-level expression of GPCRs in the Baculovirus/Sf9 cell expression system
* Expression of GPCRs in Drosophila Schneider 2 cells
* Methods for genetic analysis and ligand identification using heterologous GPCRsexpressed in Saccharomyces cerevisiae
Supplemented with numerous photographs and illustrations, Identification and Expression of G Protein-Coupled Receptors is important reading for biochemists, pharmacologists, neuroscientists, structural biologists, and anyone involved in GPCR-based research. It delivers a wealth of useful advice, practical tips, and invaluable insight into trends at the cutting-edge of current research. Also in this series: Receptor Localization: Laboratory Methods and Procedures edited by Marjorie A. Ariano

Human cloning is a prospect the contributors to Clones and Clones view with varying degrees of alarm, calm, ambivalence, and not a little humor. Ranging from psychoanalyst Adam Phillips's case study of a child whose confusion of "cloning" and "clothing" expresses our mixed desire and terror of sameness, to Stephen Jay Gould's and Richard Dawkins's "characteristically pithy and intelligent" essays (Civilization); from William Ian Miller's analysis of the queasiness the subject elicits in many of us, to Martha Nussbaum's witty and elegiac fantasy of the cloning of a lost lover-this superb collection limns our beliefs and concerns about what it means to be human. The writers here, says the San Diego Union-Tribune, "comprise an eclectic group, but their observations on the science and ethics of cloning, how it might fit into and affect human society and what the future might bring are just the sort of thinking that . . . we need more of." Praise for Clones and Clones: "A worthy exploration of a discomfiting topic." - Foreign Affairs "Greatly aid[s] the cloning debate." - Washington Post "The spectrum of authors and their varying perspectives in fact and fiction are assets to anyone who hopes to understand this broad issue and its vast cultural implications." - Publishers Weekly

Little more than a decade ago the term 'genetic engineering' was hardly known outside research laboratories. By now, though, its use is widespread. Those in favour of genetic engineering - and those against it - tell us that it has the potential to change our lives perhaps more than any other scientific or technological advance. But what are the likely consequences of genetic engineering? Is it ethically acceptable? Should we be trying to improve on nature? The authors, a biologist and a moral philosopher, examine the implications of genetic engineering in every aspect of our lives. The underlying science is explained in a way easily understood by a general reader, and the moral and ethical considerations that arise are fully discussed. Throughout, the authors clarify the issues involved so that readers can make up their own minds about these controversial issues.

The Human Genome Project, the most ambitious biological research program ever undertaken, was born in controversy. Heralded by its more enthusiastic proponents as a quest for the 'Holy Grail of biology' - and the key, ultimately, to the treatment of a variety of hereditary diseases - it has as its initial goal the mapping of all the genes in the entire three-billion-letter genetic code embodied in the DNA of a typical human cell. A major factor in the counterarguments of its opponents: its projected cost, estimated to run into the billions of dollars, spread over 10-20 years. In this firsthand account of the protracted struggle to launch the genome project, a close observer of that process - and sometime participant in it - unravels the tangled scientific and political threads of the story, relying on primary documents gathered even as events unfolded, supplemented by interviews with all the main actors - including the controversial first head of the National Institutes of Health genome effort, Nobel laureate James D. Watson. The result is an absorbing case study in the politics of modern science - focused in this case on a project with far-reaching medical and social implications.

The early promise of genetic engineering triggered a host of social and political concerns. In Molecular Politics, Susan Wright draws on government records, archival materials, and a wide range of interviews to analyze how the American and British governments responded to these concerns and to the struggles among corporations, scientists, universities, trade unions, and public interest groups for control of this controversial technology. Advancing an original approach to the expression of power in policymaking, she provides the first comparative study of a crucial set of policy decisions and explores their implications for the political economy of contemporary science.

The creation of 'test--tube babiesa acted as a spur to public debate about the implications of research on embryos, in vitro fertilization, surrogate motherhood, and the whole range of technologies concerned with human reproduction. The scope of reproductive technologies examined in this volume -- from techniques for the medical 'managementa of childbirth, to genetic engineering -- is such that few women in the western world, and smaller and smaller numbers in the third world, escape their influence. What then is their impact: on the process of reproduction, on family life and particularly on women? 'Reproductive Technologiesa is a remarkable collection of original essays which attempts to place the current controversy over reproductive technologies in a political, legal and economic context. Contributors -- including Lesley Doyal, Ann Oakley, Ros Petchesky, Carol Smart, Hilary Rose, and Naomi Pfeffer -- examine systematically the technologies that have sparked off these debates. They explore the problem of infertility which is used to validate reproductive technologies; the way assumptions about the family and about biological parenthood continue to structure the arguments for and against; the impact of the medicalization of childbirth; the way debates are embedded in changing conceptions of paternal rights, maternal rights and embryo rights; the problems of providing adequate health care for women; and, above all, the urgency with which these issues raise problems about the accountability of science.

Rice has been chosen as a model cereal for functional genomics because of its well understood genetics. In addition, there is great similarities among all the cereals and grasses: any understanding of rice genes is directly applicable to the genes of other cereals. This book details rice functional genomics. It takes a multi-pronged genome-wide approach using structural similarities, expression profiles, and mutant phenotypes. Coverage includes the current status of genome sequencing and annotation, various tools, and resources being developed worldwide.

Early characterization of toxicity and efficacy would significantly impact the overall productivity of pharmaceutical R&D and reduce drug candidate attrition and failure. By describing the available platforms and weighing their relative advantages and disadvantages, including microarray data analysis, Genomics in Drug Discovery and Development introduces readers to the biomarker, pharmacogenomic, and toxicogenomics toolbox. The authors provide a valuable resource for pharmaceutical discovery scientists, preclinical drug safety department personnel, regulatory personnel, discovery toxicologists, and safety scientists, drug development professionals, and pharmaceutical scientists.

This is the first time that human organs, such as the heart, liver, kidney, stomach, uterus, skin, lung, pancreas and breast can be manufactured automatically and precisely for clinical transplantation, drug screening and metabolism model establishment. Headed by Professor Xiaohong Wang (also the founder and director) in the Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, this group has focused on organ manufacturing for over ten years. A series of technical bottleneck problems, such as vascular and nerve system establishment in a construct, multiple cell types and material system incorporation, and stem cell sequential engagement, have been overcome one by one. Two technical approaches have been exploited extensively. One is multiple nozzle rapid prototyping (RP), additive manufacturing (AM), or three-dimension (3D) printing. The other is combined mold systems. More than 110 articles and 40 patents with a series of theories and practices have been published consequently. In the future, all the failed organs (including the brain) in the human body can be substituted easily like a small accessory part in a car. Everyone can get benefit from these techniques, which ultimately means that the lifespan of humans, therefore, can be greatly prolonged from this time point. This book examines the progress made in the field and the developments made by these researchers (and authors) in the field.

Induced pluripotent stem (iPS) cell technology has paved new ways for disease modelling and drug discovery. Disease modelling with the differentiated neuronal cells from patient-specific iPS cells partially recapitulated the phenotypes of spinal muscular atrophy, familial dysautonomia and Rett syndrome. In this book, the authors present current research in the study of induced stem cells, including the cryopreservation of pluipotent stem cells; tissue engineering approaches using bioactive ceramics towards bone regeneration; induced pluripotent stem cell-derived hepatocytes as an alternative to human adult hepatocytes; iPS technology for studying neurodegenerative diseases and iPS from cord blood cells.

The long-awaited revision of the industry standard on phylogenetics

Since the publication of the first edition of this landmark volume more than twenty-five years ago, phylogenetic systematics has taken its place as the dominant paradigm of systematic biology. It has profoundly influenced the way scientists study evolution, and has seen many theoretical and technical advances as the field has continued to grow. It goes almost without saying that the next twenty-five years of phylogenetic research will prove as fascinating as the first, with many exciting developments yet to come.

This new edition of "Phylogenetics" captures the very essence of this rapidly evolving discipline. Written for the practicing systematist and phylogeneticist, it addresses both the philosophical and technical issues of the field, as well as surveys general practices in taxonomy. Major sections of the book deal with the nature of species and higher taxa, homology and characters, trees and tree graphs, and biogeography--the purpose being to develop biologically relevant species, character, tree, and biogeographic concepts that can be applied fruitfully to phylogenetics.

The book then turns its focus to phylogenetic trees, including an in-depth guide to tree-building algorithms. Additional coverage includes:

Parsimony and parsimony analysis

Parametric phylogenetics including maximum likelihood and Bayesian approaches

Phylogenetic classification

Critiques of evolutionary taxonomy, phenetics, and transformed cladistics

Specimen selection, field collecting, and curating

Systematic publication and the rules of nomenclature

Providing a thorough synthesis of the field, this important update to "Phylogenetics" is essential for students and researchers in the areas of evolutionary biology, molecular evolution, genetics and evolutionary genetics, paleontology, physical anthropology, and zoology.