What does a mammoth smell like? Do dinosaurs bob their heads as they walk, like today’s birds? Do aurochs moo like cows? You may soon find out.

From the Siberian permafrost to balmy California, scientists across the globe are working to resurrect all kinds of extinct animals, from ones that just left us to those that have been gone for many thousands of years. Their tools in this hunt are both fossils and cutting-edge genetic technologies. Some of these scientists are driven by sheer curiosity; others view the lost species as a powerful weapon in the fight to save rapidly disappearing ecosystems.

Science journalist Torill Kornfeldt travelled the world to meet the men and women working to bring extinct animals back from the dead. Along the way, she saw a mammoth that has been frozen for 20,000 years, and visited the places where these furry giants once walked. It seems certain that they and other lost species will walk the earth again, but what world will that give us? And is any of this a good idea?

The process of discovery in science and technology may require investigation of a large number of features, such as factors, genes or molecules. In Screening, designed experiments and statistical analyses of the resulting data sets are used to identify efficiently the few features that determine key properties of the system under study.

This book brings together accounts by leading international experts that are essential reading for those working in fields such as industrial quality improvement, engineering research and development, genetic and medical screening, drug discovery, and computer simulation of manufacturing systems or economic models. Our aim is to promote cross-fertilization of ideas and methods through detailed explanations, a variety of examples and extensive references.

Topics cover both physical and computer simulated experiments. They include screening methods for detecting factors that affect the value of a response or its variability, and for choosing between various different response models. Screening for disease in blood samples, for genes linked to a disease and for new compounds in the search for effective drugs are also described. Statistical techniques include Bayesian and frequentist methods of data analysis, algorithmic methods for both the design and analysis of experiments, and the construction of fractional factorial designs and orthogonal arrays.

The material is accessible to graduate and research statisticians, and to engineers and chemists with a working knowledge of statistical ideas and techniques. It will be of interest to practitioners and researchers who wish to learn about useful methodologies from within their own areaas well as methodologies that can be translated from one area to another.

What should individuals and society do when genetic screening becomes widely available and with its impact on current and future generations still uncertain? How can our education systems around the world respond to these developments? Reproductive and genetic technologies (RGTs) are increasingly controversial and political. We are entering an era where we can design future humans, firstly, by genetic screening of "undesirable" traits or indeed embryos, but perhaps later by more radical genetic engineering. This has a profound effect on what we see as normal, acceptable and responsible. This book argues that these urgent and biopolitical issues should be central to how biology is taught as a subject. Debate about life itself has always been at the forefront of connected molecular, genetic and social/personal identity levels, and each of these levels requires processes of communication and debate, what Anthony Giddens called in passing life politics. In this book Padraig Murphy opens the term up, with examples from field research in schools, student responses to educational films exploring the future of RGTs, and science studies of strategic biotechnology and the lab practices of genetic screening. Life political debate is thoroughly examined and is identified as a way of connecting mainstream education of biology with future generations. Biotechnology, Education and Life Politics will appeal to post-graduates and academics involved with science education, science communication, communication studies and the sociology of education.

DNA, once the exclusive domain of scientists in research labs, is now the darling of popular and social media. With personal genetic testing kits in homes and GMO foods in stores, DNA is an increasingly familiar term. Unfortunately, what people know, or think they know, about DNA and genetics is often confused or incorrect. Contrary to popular belief, for instance, genes don't "skip a generation" and, no, human DNA is not "different" from DNA of other species. With popular misconceptions proliferating in the news and on the internet, how can anyone sort fact from fiction? DNA Demystified satisfies the public appetite for and curiosity about DNA and genetics. Alan McHughen, an accomplished academic and public science advocate, brings the reader up-to-speed on what we know, what we don't, and where genetic technologies are taking us. The book begins with the basic groundwork and a brief history of DNA and genetics. Chapters then cover newsworthy topics, including DNA fingerprinting, using DNA in forensic analyses, and identifying cold-case criminals. For readers intrigued by the proliferation of at-home DNA tests, the text includes fascinating explorations of genetic genealogy and family tree construction-crucial for people seeking their biological ancestry. Other chapters describe genetic engineering in medicine and pharmaceuticals, and the use of those same technologies in creating the far more controversial GMOs in food and agriculture. Throughout, the book raises provocative ethical and privacy issues arising from DNA and genetic technologies. With the author's comprehensive expertise, DNA Demystified offers an informal yet authoritative guide to the genetic marvel of DNA.

Each plant species has its own unique passage that is affected by its gene pool, dispersal ability, interactions with competitors and pests, and the habitats and climactic conditions to which it is exposed. This book will explore plant species as dynamic entities within this passage, following the four stages of plant species life that normally occur. Those four stages can be identified as birth, expansion, differentiation and loss of cohesion, and decline/extinction. Each chapter focuses on part of the speciation process and examines it closely in the light of exploring the species passage from birth to death.

This book presents descriptive overviews of gene editing strategies across multiple species while also offering in-depth insight on complex cases of application in the field of tissue engineering and regenerative medicine. Chapters feature contributions from leaders in stem cell therapy and biology, providing a comprehensive view of the application of gene therapy in numerous fields with an emphasis on ophthalmology, stem cells, and agriculture. The book also highlights recent major technological advances, including ZFN, TALEN, and CRISPR. Precision Medicine, CRISPR, and Genome Engineering is part of the highly successful Advances in Experimental Medicine and Biology series. It is an indispensable resource for researchers and students in genetics as well as clinicians.

It was through control of the shattering of wild seeds that humans first domesticated plants. Now control over those very plants threatens to shatter the world's food supply, as loss of genetic diversity sets the stage for widespread hunger. Large-scale agriculture has come to favor uniformity in food crops. More than 7,000 U.S. apple varieties once grew in American orchards; 6,000 of them are no longer available. Every broccoli variety offered through seed catalogs in 1900 has now disappeared. As the international genetics supply industry absorbs seed companies--with nearly one thousand takeovers since 1970--this trend toward uniformity seems likely to continue; and as third world agriculture is brought in line with international business interests, the gene pools of humanity's most basic foods are threatened. The consequences are more than culinary. Without the genetic diversity from which farmers traditionally breed for resistance to diseases, crops are more susceptible to the spread of pestilence. Tragedies like the Irish Potato Famine may be thought of today as ancient history; yet the U.S. corn blight of 1970 shows that technologically based agribusiness is a breeding ground for disaster. "Shattering" reviews the development of genetic diversity over 10,000 years of human agriculture, then exposes its loss in our lifetime at the hands of political and economic forces. The possibility of crisis is real; this book shows that it may not be too late to avert it.

In recent decades, livestock producers have moved away from open grazing for a number of reasons, none having to do with the health of consumers. Genetic Resources, Chromosome Engineering, and Crop Improvement: Forage Crops demonstrates how state-of-the-art technology can encourage the raising of livestock in open pastures where they can be fed grasses grown in nature rather than meals enriched with hormones and other by-products. The volume brings together the world's leading innovators in crop science who furnish information on the availability of germplasm resources that breeders can exploit for the improvement of major forage crop varieties including alfalfa, wheatgrass and wildrye grasses, Bahiagrass, birdsfoot trefoil, clover, Bermudagrass, and ryegrass. An introductory chapter outlines the cytogenetic architecture of forage crops, describes the principles and strategies of cytogenetic and breeding manipulations, and summarizes landmark research. Ensuing chapters provide a comprehensive account of each crop: its origin; wild relatives; exploitation of genetic resources in the primary, secondary, and tertiary, and, where feasible, quarternary gene pools through breeding and cytogenetic manipulation; and genetic enrichment using the tools of molecular genetics and biotechnology. . Certain to become the standard reference, this volume- Discusses taxonomy, genomic and chromosomal constitution, and the geographical distribution Stresses the role of germplasm exploration, maintenance, and assimilation for increasing yield Presents practical improvement methodologies including conventional, cytogenetic, mutation, molecular, cell and tissue cultures, and genetic transformation In addition to serving as fodder, forage crops provide ground cover, aid in abetting erosions, yield a number of pharmaceuti

Efforts to improve food security in the developing world have been hampered due to myths surrounding GM agriculture. This book explores the theory, evidence and rhetoric of the impact of food production on the environment, and the impact of the environment on food production. The chapters address: food security and technology; expertise and opportunism; the promise of technology; the politicization of risk; industrial agriculture; the meaning of 'natural'; the potential of the local food movement; food labelling; genetic diversity in the agro-industrial era; sustainability and chemical application; plant vitality; and future prospects for food security. Each chapter includes a personal introduction from the authors about the issues at hand, followed by a detailed analysis with further references. The book considers the origins of concerns and then examines the evidence around the issues, and the impacts in terms of policy, regulation and agricultural practice. It also: - Refutes common consumer and environmental organization myths about biotechnology. - Highlights the importance of food security in both the developing and developed world. - Provides a pro-science approach to increasing food security. This book will be of interest to students and researchers in biotechnology, food security and public understanding of science, and also to policy makers, regulators and industry managers.

"Sandel explores a paramount question of our era: how to extend the power and promise of biomedical science to overcome debility without compromising our humanity. His arguments are acute and penetrating, melding sound logic with compassion." -Jerome Groopman, author of How Doctors Think Breakthroughs in genetics present us with a promise and a predicament. The promise is that we will soon be able to treat and prevent a host of debilitating diseases. The predicament is that our newfound genetic knowledge may enable us to manipulate our nature-to enhance our genetic traits and those of our children. Although most people find at least some forms of genetic engineering disquieting, it is not easy to articulate why. What is wrong with re-engineering our nature? The Case against Perfection explores these and other moral quandaries connected with the quest to perfect ourselves and our children. Michael Sandel argues that the pursuit of perfection is flawed for reasons that go beyond safety and fairness. The drive to enhance human nature through genetic technologies is objectionable because it represents a bid for mastery and dominion that fails to appreciate the gifted character of human powers and achievements. Carrying us beyond familiar terms of political discourse, this book contends that the genetic revolution will change the way philosophers discuss ethics and will force spiritual questions back onto the political agenda. In order to grapple with the ethics of enhancement, we need to confront questions largely lost from view in the modern world. Since these questions verge on theology, modern philosophers and political theorists tend to shrink from them. But our new powers of biotechnology make these questions unavoidable. Addressing them is the task of this book, by one of America's preeminent moral and political thinkers.

This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence "(lux)" genes from the marine bacterium "Vibrio fischeri." The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.

Over the last two decades one of the great global emerging technological trends has been the shift from chemistry to biology in agriculture. Bitterly contested and enduringly controversial, the shift to biotechnology has nevertheless led to greater sustainability and promises even greater gains in years to come. This Handbook is an invaluable compendium of detailed case study and insight.' - Mark Lynas, Cornell University, US'This important volume analyses the current state of crop biotechnology development and regulation. It establishes a firm basis for understanding the current level of deployment of crops modified by biotechnology and also the uneven and often unscientific bases that have been used to judge their merits for particular regions. This book is an indispensable reference for anyone concerned with the development of this vital area of agriculture.' - Peter H. Raven, Missouri Botanical Garden, US 'With interest in biotechnology surging, this book is a must-read for anyone who wants to learn more about the potential that advancements in modern agriculture have to offer, how they've already changed agriculture around the world and what s coming down the pipeline. Agriculture is about economics, the environment and feeding the world; so too, at the heart of it, is biotechnology, as this book so clearly demonstrates. The authors have years of experience with biotechnology and their expertise shines through on each page.' - Lorne Hepworth, CropLife Canada This book is a compendium of knowledge, experience and insight on agriculture, biotechnology and development. Beginning with an account of GM crop adoptions and attitudes towards them, the book assesses numerous crucial processes, concluding with detailed insights into GM products. Drawing on expert perspectives of leading authors from 57 different institutions in 16 countries, it provides a unique, global overview of agbiotech following 20 years of adoption. Many consider GM crops the most rapid agricultural innovation adopted in the history of agriculture. This book provides insights as to why the adoption has occurred globally at such a rapid rate. This is a rich and varied collection of research, which will appeal to scholars, academics and practitioners worldwide. An invaluable resource, this book will be a first point of reference to anyone with an interest in agbiotech and studies into agriculture, biotechnology and development. Contributors: A.A. Adenle, P. Aerni, C. Alexander, J.M. Alston, V. Beckmann, J. Bognar, C.G. Borroto, D. Brewin, G. Brookes, J. Carpenter, Y. Carriere, D. Castle, M. Chen, P. Conceicao, B. Dayananda, M. Demont, K. Dillen, D. Eaton, E. Einsiedel, J. Falck-Zepeda, J. Fernandez-Cornejo, G.B. Frisvold, C.V. Gonslaves, D. Gonsalves, M. Gouse, G. Graff, R. Gray, A. Gupta, W.O. Hennessey, J.E. Hobbs, W.E. Huffman, L. A. Jackson, C. Juma, N. Kalaitzandonakes, S. Kaplan, V.J. Karplus, W.A. Kerr, G.G. Khachatourians, E.M. Kikulwe, E. Kim, D.E. Kolady, S.P. Kowalski, J. Kruse, L. Levidow, S. Levine, K. Ludlow, X. Ma, A. Magnier, S. Malla, I. Matuschke, J.J. McCluskey, A. McHughen, J. Medlock, D. Miller, L. Nagarajan, A. Naseem, C. Oguamanam, M. Ouattarra, M. Owen, R. Paarlberg, P. W. B. Phillips, M. Qaim, T. Raney, J.M. Reeves, S.D. Rhodes, S.M.H. Rizvi, C.D. Ryan, D. Schimmelpfennig, G.J. Scoles, G. Skogstad, S. J. Smyth, C. Soregaroli, D.J. Spielman, A.J. Stein, J. Thomson, J. Vitale, G. Vognan, G. Waterfield, S. Wechsler, J. Wesseler, A. Williams, W.W. Wilson, L.L. Wolfenbarger, G. Ye, J. Yorobe Jr, D.Z. Zeng, D. Zilberman

Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications.

Much research has focused on the basic cellular and molecular biological aspects of stem cells. Much of this research has been fueled by their potential for use in regenerative medicine applications, which has in turn spurred growing numbers of translational and clinical studies. However, more work is needed if the potential is to be realized for improvement of the lives and well-being of patients with numerous diseases and conditions. This book series 'Cell Biology and Translational Medicine (CBTMED)' as part of SpringerNature's longstanding and very successful Advances in Experimental Medicine and Biology book series, has the goal to accelerate advances by timely information exchange. Emerging areas of regenerative medicine and translational aspects of stem cells are covered in each volume. Outstanding researchers are recruited to highlight developments and remaining challenges in both the basic research and clinical arenas. This current book is the sixth volume of a continuing series.

The Mutator Transposable Element Family of Maize.- Protein Phosphorylation and the Regulation of Cellular Processes by the Homologous Two-Component Systems of Bacteria.- The Peculiar Nature of Codon Usage in Primates.- The Role of Nodulation Genes in Bacterium-Plant Communication.- Regulation of Gene Expression by Epidermal Growth Factor.- Machinery for Protein Import into Chloroplasts and Mitochondria.- High-Level Expression of Foreign Genes in Mammalian Cells.- Aromatic Hydrocarbon Degradation: A Molecular Approach.- Employment of Fibroblasts for Gene Transfer Applications for Grafting into the Central Nervous System.- The Molecular Biology of Amino Acid Biosynthesis in Plants.- Genetic Manipulation of Bacillus Thuringiensis Insectidal Crystal Protein Genes in Bacteria.- Progress Towards Gene Targeting in Plants.- Molecular Biology of Mating-Type Determination in Schizophyllum Commune.- Functions of Intracellular Protein Degradation in Yeast.- Transgenic Fish for Aquaculture.

This book presents a historicised account of the Feminist International Network of Resistance to Reproductive and Genetic Engineering (FINRRAGE), a coordinated effort during the 1980s and 1990s by an international group of women to create and disseminate feminist knowledge about the then-new field of reproductive technologies. Bringing insights from science and technology studies together with social movements and feminist theory, it seeks to examine larger questions about knowledge and expertise in activist engagements with rapidly-developing technologies, as well as explore an important and neglected episode of feminist history. Its findings will be relevant to scholars in science studies, gender and women's studies and social movements, as well as to anyone with an interest in reproductive technologies and the history of feminist activism.

A vision of the future where the latest Silicon Valley tech meets cutting-edge genetics. Decoding the World is a buddy adventure about the quest to live meaningfully in a world with such uncertainty. It starts with Po Bronson coming to IndieBio. Arvind Gupta created IndieBio as a laboratory for early biotech startups trying to solve major world problems. Glaciers melting. Dying bees. Infertility. Cancer. Ocean plastic. Pandemics. As they travel around the world, finding scientists to join their cause, the authors bring their first-hand experience to the great mysteries that haunt our future. Natural resource depletion. Job-taking robots. China's global influence. Decoding the World is the kind of book you get when you give two guys $40 million, a world full of messy big problems, a genetics laboratory to play in, and a set of Borges' collected works. After looking through their lens, you'll never see the world the same.

This book covers trends in modern biotechnology. All aspects of this interdisciplinary technology, where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science, are treated. More information as well as the electronic version is available at springer.com.

A comprehensive and mechanistic perspective on fruit ripening, emphasizing commonalities and differences between fruit groups and ripening processes.

Fruits are an essential part of the human diet and contain important phytochemicals that provide protection against heart disease and cancers. Fruit ripening is of importance for human health and for industry-based strategies to harness natural variation, or genetic modification, for crop improvement.

This book covers recent advances in the field of plant genomics and how these discoveries can be exploited to understand evolutionary processes and the complex network of hormonal and genetic control of ripening. The book explains the physiochemical and molecular changes in fruit that impact its quality, and recent developments in understanding of the genetic, molecular and biochemical basis for colour, flavour and texture. It is a valuable resource for plant and crop researchers and professionals, agricultural engineers, horticulturists, and food scientists.

Summary: Reviews the physiochemical and molecular changes in fruit which impact flavour, texture, and colourCovers recent advances in genomics on the genetic, molecular, and biochemical basis of fruit qualityIntegrates information on both hormonal and genetic control of ripeningRelevant for basic researchers and applied scientists

This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

Computational Epigenetics and Diseases, written by leading scientists in this evolving field, provides a comprehensive and cutting-edge knowledge of computational epigenetics in human diseases. In particular, the major computational tools, databases, and strategies for computational epigenetics analysis, for example, DNA methylation, histone modifications, microRNA, noncoding RNA, and ceRNA, are summarized, in the context of human diseases. This book discusses bioinformatics methods for epigenetic analysis specifically applied to human conditions such as aging, atherosclerosis, diabetes mellitus, schizophrenia, bipolar disorder, Alzheimer disease, Parkinson disease, liver and autoimmune disorders, and reproductive and respiratory diseases. Additionally, different organ cancers, such as breast, lung, and colon, are discussed. This book is a valuable source for graduate students and researchers in genetics and bioinformatics, and several biomedical field members interested in applying computational epigenetics in their research.

Summarizing landmark research, Volume 2 of this essential series furnishes information on the availability of germplasm resources that breeders can exploit for producing high-yielding cereal crop varieties. Written by leading international experts, this volume offers the most comprehensive and up-to-date information on employing genetic resources to increase the yield of those cereal crops that provide the main source of nutrition for two-thirds of the world. In thirteen succinct chapters, Genetic Resources, Chromosome Engineering, and Crop Improvement: Cereals, Volume 2 focuses on wheat, rice, maize, oats, barley, millet, sorghum, and rye, as well as triticale: a wheat and rye hybrid with great potential. An introductory chapter outlines the cytogenetic architecture of cereal crops, describes the principles and strategies of cytogenetics and breeding, and summarizes landmarks in current research. This sets the stage for the ensuing crop-specific chapters. Each chapter generally provides a comprehensive account of the crop, its origin, wild relatives, exploitation of genetic resources in the primary, secondary, and tertiary gene pools through breeding and cytogenetic manipulation, and genetic enrichment using the tools of molecular genetics and biotechnology. Certain to become the standard reference for improving the yields of these critical grains, this book is the definitive source of information for plant breeders, agronomists, cytogeneticists, taxonomists, molecular biologists, biotechnologists, and graduate students and researchers in these fields.

Louis-Marie Houdebine and Jianglin Fan The study of biological functions of proteins and their possible roles in the pathogenesis of human diseases requires more and more relevant animal m- els. Although mice including genetically modified mice offer many possibilities, other non-murine species are absolutely required in some circumstances. Rabbit is one of these species, which has been widely used in biomedical studies. This animal is genetically and physiologically closer to humans including cardiov- cular system and metabolism characteristics. Rabbit is thus more appropriate than mice to study some diseases such as atherosclerosis and lipid metabolism. Because of its larger size, surgery manipulation, bleeding, and turn-over studies are much easier performed in rabbits than in mice. Furthermore, transgenic rabbits can be produced using microinjection and other methods such as lentiviral v- tors. Cloning in rabbits has been proved possible, even though still laborious and time-consuming. Hopefully, functional rabbit ES cell lines will be available in the coming years. Gene deletion or knock-out in rabbits will then become possible.

Transhumanists advocate for the development and distribution of technologies that will enhance human intellectual, physical, and psychological capacities, even eliminate aging. What if the dystopian futures and transhumanist utopias found in the pages of science journals, Margaret Atwood novels, films like "Gattaca," and television shows like "Dark Angel" are realized? What kind of world would humans have created?

Maxwell J. Mehlman considers the promises and perils of using genetic engineering in an effort to direct the future course of human evolution. He addresses scientific and ethical issues without choosing sides in the dispute between transhumanists and their challengers. However, "Transhumanist Dreams and Dystopian Nightmares" reveals that radical forms of genetic engineering could become a reality much sooner than many people think, and that we need to encourage risk-management efforts.

Whether scientists are dubious or optimistic about the prospects for directed evolution, they tend to agree on two things. First, however long it takes to perfect the necessary technology, it is inevitable that humans will attempt to control their evolutionary future, and second, in the process of learning how to direct evolution, we are bound to make mistakes. Our responsibility is to learn how to balance innovation with caution.

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field.