The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book.

Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.

The author of the international bestseller The Sixth Extinction returns to humanity's transformative impact on the environment, now asking: after doing so much damage, can we change nature, this time to save it? Meet the biologists trying to save the world's rarest fish; the engineers who are turning carbon emissions to stone; the researchers trying to develop a 'super coral'; and the physicists contemplating shooting tiny diamonds into the stratosphere to cool the earth. Elizabeth Kolbert is one of the most important writers on the environment. Here she investigates the immense challenges humanity faces as we scramble to reverse, in a matter of decades, the effects we've had on the natural world and asks - can we save the natural world in time? 'Important, necessary, urgent' Helen MacDonald 'Meticulously researched and deftly crafted' Guardian

The announcement last spring that a lab in Scotland had successfully cloned a mammal captured the attention of the media and the imagination of the public. This culmination of decades of research has profound scientific and ethical implications. If applied to other species, cloning could further genetic engineering and greatly improve animal husbandry. Now that a sheep has been cloned, are humans next? Governments reacted swiftly with bans on funding for human cloning research. Churches united in calling for a complete ban on the cloning of higher animals. Critics immediately alluded to Mary Shelley's "Frankenstein" and the myth of Icarus. Has scientific sophistication outpaced our social and moral development? Can we "save" our society from this possible evil by banning any attempts to expand the knowledge? Does cloning really differ in spirit from the selective breeding that humankind has performed for centuries? "Cloning: For and Against" comprises 30 articles by scientists, ethicists, religious leaders and legal experts who explore the benefits and costs of cloning. Topics include: playing God: is cloning against human nature?; is cloning the salvation for endangered species?; no need for marriage: the separation of reproduction from human relationships; can you xerox a soul? and other theological issues; Brave New World: what's possible and what isn't; clones in medicine; and a million Michael Jacksons: eugenic/dysgenic and cultural consequences of human cloning.

In 2001 the Human Genome Project succeeded in mapping the DNA of humans. This landmark accomplishment launched the field of genomics, the integrated study of all the genes in the human body and the related biomedical interventions that can be tailored to benefit a person's health. Today genomics, part of a larger movement toward personalized medicine, is poised to revolutionize health care. By cross-referencing an individual's genetic sequence - their genome - against known elements of "Big Data," elements of genomics are already being incorporated on a widespread basis, including prenatal disease screening and targeted cancer treatments. With more innovations soon to arrive at the bedside, the promise of the genomics revolution is limitless. This entry in the What Everyone Needs to Know series offers an authoritative resource on the prospects and realities of genomics and personalized medicine. As this science continues to alter traditional medical paradigms, consumers are faced with additional options and more complicated decisions regarding their health care. This book provides the essential information everyone needs.

The decline of many individual and wild fish stocks has commanded an increase in aquaculture production to meet the protein demands of a growing population. Alongside selective breeding schemes and expanding facilities, transgenic methods have received increasing attention as a potential factor in meeting these demands. With a focus on developing countries, this third text in the series provides detailed information on environmental biosafety policy and regulation and presents methodologies for assessing ecological risks associated with transgenic fish.

This book provides a timely overview of toxicogenomics, with special emphasis on the practical applications of this technology to the risk assessment process. Introductory sections are followed by a series of chapters highlighting practical and systematic applications of toxicogenomics in informing the risk assessment process - including the areas of mutagenicity, carcinogenicity, endocrine toxicity, organ-specific toxicity, population monitoring, and ecotoxicology. The book concludes with approaches for the integration of this technology in safety evaluation studies, and an outlook on how toxicogenomics and complementary technologies can reframe the current risk assessment paradigm.

In China, as elsewhere, the debate over genetically modified organisms has become polarized into anti- and pro-GMO camps. Given the size of China's population and market, much is at stake in conflicts over regulation for domestic as well as international actors. In this book, Cong Cao provides an even-handed analysis that illuminates the tensions that have shaped China's policy toward agricultural biotechnology in a global perspective. Cao presents a comprehensive and systematic analysis of how China's policy toward research and commercialization of genetically modified crops has shifted that explains how China's changing GMO stances reflect its evolving position on the world stage. While China's scientific community has set the agenda, it has encountered resistance rooted in concerns over food safety and consumers' rights as well as issues of intellectual property rights and food sovereignty. Although Chinese leaders at first sought to take advantage of the biotech revolution by promoting GMO crop consumption, Cao demonstrates that policy has since become precautionary, as seen in new laws and regulations grounded in concerns over safety and the deferral of commercialization of GM rice. He presents China's policies in light of changing global attitudes toward GM crops: As shifts in China have closely followed global trends, so has domestic activism. Drawing on government and scientific documents as well as interviews with scientists, officials, policy analysts, activists, and journalists, GMO China is an important book for China studies, science and technology studies, policy analysts, and professionals interested in the Chinese biotechnology market.

Probablistic models are becoming increasingly important in analyzing the huge amount of data being produced by large-scale DNA-sequencing efforts such as the Human Genome Project. For example, hidden Markov models are used for analyzing biological sequences, linguistic-grammar-based probabilistic models for identifying RNA secondary structure, and probabilistic evolutionary models for inferring phylogenies of sequences from different organisms. This book gives a unified, up-to-date and self-contained account, with a Bayesian slant, of such methods, and more generally to probabilistic methods of sequence analysis. Written by an interdisciplinary team of authors, it is accessible to molecular biologists, computer scientists, and mathematicians with no formal knowledge of the other fields, and at the same time presents the state of the art in this new and important field.

Would you change your genes if you could? As we confront the 'industrial revolution of the genome', the recent discoveries of Crispr-Cas9 technologies are offering, for the first time, cheap and effective methods for editing the human genome. This opens up startling new opportunities as well as significant ethical uncertainty. Tracing events across a fifty-year period, from the first gene splicing techniques to the present day, this is the story of gene editing - the science, the impact and the potential. Kozubek weaves together the fascinating stories of many of the scientists involved in the development of gene editing technology. Along the way, he demystifies how the technology really works and provides vivid and thought-provoking reflections on the continuing ethical debate. This updated paperback edition contains all the very latest on the patent battle over Crisp and the applications of Crispr technology in agriculture and medicine.

Many farmers in sub-Saharan Africa suffer heavily from crop losses due to stem borer pests. Insecticides are often unaffordable; therefore, maize plants must be made resistant to pests. The 'Insect Resistant Maize for Africa' (IRMA) project in Kenya was aimed at developing new maize varieties both by conventional methods and by biotechnologically incorporating the ?-endotoxin produced by the soil bacterium Bacillus thuringiensis. The author gives an impartial and chronological account of this exemplary project between 1999 and 2008, supplemented by discussions of agricultural development policy and descriptions of Kenyan smallholders and the project team. Taking critical and rational positions on the use of modern plant breeding techniques, biotechnology and development policy, this book is of interest to researchers and students, development agencies, NGOs and biotechnology companies.

The genetic modification of crops continues to be the subject of intense debate, and opinions are often strongly polarised. "Environmental Impact of Genetically Modified Crops" addresses the major concerns of scientists, policy makers, environmental lobby groups and the general public regarding this controversial issue, from an editorially neutral standpoint. While the main focus is on environmental impact, food safety issues, for both humans and animals are also considered. The book concludes with a discussion on the future of agricultural biotechnology in the context of sustainability, natural resource management and future global population and food supply.

Many international forums have identified the need for comprehensive, scientific methods for the pre-release testing and post-release monitoring of transgenic plants to ensure their environmental safety and sustainable use. In response to this requirement, a GMO Guidelines Project was established under the aegis of the International Organization for Biological Control, to develop biosafety testing guidelines for transgenic plants. This second volume focuses on transgenic cotton in Brazil and addresses both environmental and agricultural impacts. It draws out some general risk assessment guidelines and demonstrates the need for case-by-case analysis.

Continued advances in biotechnology have driven forward the harnessing of micro-organisms and plants to help protect our fragile environment from the impact of human activity in increasingly targeted yet diverse ways. Environmental Biotechnology provides a broad overview of the subject, focusing on how biotechnological techniques are applied to solve environmental problems, rather than giving detailed explanations of the techniques themselves. Opening with an overview of environmental biotechnology, and a review of the key aspects of microbiology upon which the field is based, the book goes on to explore the diverse way in which biotechnology is applied to tackle environmental problems and issues, from monitoring of the environment and treatment of waste, to the removal of pollutants and extraction of oil and minerals. The book closes with a discussion of the ways in which future advances in biotechnology are likely to be harnessed to address current and emerging issues and problems in increasingly effective and powerful ways. Capturing the current excitement in a field reinvigorated by advances in genetic manipulation, and emerging genomic and proteomic technologies, Environmental Biotechnology is the perfect resource for any student needing to develop a sound understanding of biotechnology, and the diverse ways it can be applied to address important environmental issues. Online Resource Centre - Figures from the book available to download, to facilitate lecture preparation - Library of web links cited in the book, to give students ready access to these additional resources

In 2001 the Human Genome Project succeeded in mapping the DNA of humans. This landmark accomplishment launched the field of genomics, the integrated study of all the genes in the human body and the related biomedical interventions that can be tailored to benefit a person's health. Today genomics, part of a larger movement toward personalized medicine, is poised to revolutionize health care. By cross-referencing an individual's genetic sequence - their genome - against known elements of "Big Data," elements of genomics are already being incorporated on a widespread basis, including prenatal disease screening and targeted cancer treatments. With more innovations soon to arrive at the bedside, the promise of the genomics revolution is limitless. This entry in the What Everyone Needs to Know series offers an authoritative resource on the prospects and realities of genomics and personalized medicine. As this science continues to alter traditional medical paradigms, consumers are faced with additional options and more complicated decisions regarding their health care. This book provides the essential information everyone needs.

DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA and by providing a massive parallel platform for data gathering represent a fundamental technical advance in biomedical research. This volume is a comprehensive overview of DNA microarray technology and will be invaluable to any researcher interested in taking advantage of this powerful new technique.

The late 20th century has witnessed dramatic technological developments in biomedical science and the delivery of health care, and these developments have brought with them important social changes. All too often ethical analysis has lagged behind these changes. The purpose of this series is to provide lively, up-to-date, and authoritative studies for the increasingly large and diverse readership concerned with issues in biomedical ethics - not just healthcare trainees and professionals, but also social scientists, philosophers, lawyers, social workers, and legislators. This volume brings together work by an international group of contributors from various fields and perspectives, on ethical, social, and legal issues raised by recent advances in reproductive technology. These advances have put us in a position to choose what kinds of children and parents there should be; the aim of the essays is to illuminate how we should deal with these possibilities for choice. Topics discussed include gender and race selection, genetic engineering, fertility treatment, ovarian tissue transfer, and post-menopausal pregnancy. The central focus of the volume is the interface between reproductive c

Bringing together the ideas of experts from around the world, this incisive text offers cutting-edge perspectives on the risk analysis and governance of genetically modified organisms (GMOs), supporting effective and informed decision-making in developing countries. Comprised of four comprehensive sections, this book covers: integrated risk analysis and decision making, giving an overview of the science involved and examining risk analysis methods that impact decision-making on the release of GMOs, particularly in developing countries; diversification of expertise involved in risk analysis and practical ways in which the lack of expertise in developing countries can be overcome; risk analysis based regulatory systems and how they can be undermined by power relationships and socio-political interests, as well as strategies for improving GMO policy development and regulatory decision-making; and case studies from developing countries providing lessons based on real-world experience that can inform our current thinking.

This book identifies targets for plant transformation by molecular biology for two crops of major importance in European agriculture - wheat and oilseed rape - and the potentially important protein crop faba beans. Modern techniques have enabled researchers to identify, isolate and modify plant genes, and much effort is now being devoted to improving these techniques and to adapting them to crop plants. By these means, it should prove possible to make defined changes to plants of commercial value, to improve their yield, quality and resistance to stresses, pests and diseases. This volume results from a report prepared for the Genetics and Biotechnology Division of the Commission of the European Communities by Dr Austin and his colleagues at the Plant Breeding Institute, where some of the work is being carried out. It therefore provides an authoritative account of the area for research workers and students.

mRNA METABOLISM & POST-TRANSCRIPTIONAL GENE REGULATION
Edited by Joe B. Harford and David R. Morris
Gene expression is a process that begins with the transcription of DNA to an RNA messenger (mRNA), which is then translated into a protein. Historically, attention has been focused on the regulation of RNA synthesis (transcription); however, there is a growing recognition of and appreciation for the importance of the many regulatory mechanisms that take place after RNA synthesis has been completed.
mRNA Metabolism and Post-Transcriptional Gene Regulation is the first comprehensive overview of the various modes of gene regulation that exist post-transcriptionally. Collecting studies by some of the top researchers in the field, this volume provides both an up-to-date review of the complex "life" of an mRNA molecule and an introduction to current work on the diversity of mechanisms of post-transcriptional reactions.
Topics covered include:
* RNA structure
* Mammalian RNA editing
* RNA export from the nucleus
* The fundamentals of translation initiation
* Control of mRNA decay in plants
* mRNA metabolism and cancer
* Control of mRNA stability during herpes simplex virus infection
* Regulation of mRNA expression in HIV-1 and other complex retroviruses
* Nucleases
* RNA localization

A timely contribution to the understanding of genetic regulatory mechanisms, mRNA Metabolism and Post-Transcriptional Gene Regulation provides a basis from which potential therapeutic strategies may be developed. This book will be of vital interest to cell and molecular biologists at all levels, from graduate students to senior investigators, clinical researchers, and professionals in the pharmaceutical and biotechnology industries.

Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Since the birth of civilisation, human beings have manipulated other life-forms. We have selectively bred plants and animals for thousands of years to maximise agricultural production and cater to our taste in pets. The observation of the creation of artificial animal and plant variants was a key stimulant for Charles Darwin's theory of evolution. The ability to directly engineer the genomes of organisms first became possible in the 1970s, when the gene for human insulin was introduced into bacteria to produce this protein for diabetics. At the same time, mice were modified to produce human growth hormone, and grew huge as a result. But these were only our first tottering steps into the possibilities of genetic engineering. In the past few years, the pace of progress has accelerated enormously. We can now cut and paste genes using molecular scissors with astonishing ease, and the new technology of genome editing can be applied to practically any species of plants or animals. 'Mutation chain reaction' can be used to alter the genes of a population of pests, such as flies; as the modified creatures breed, the mutation is spread through the population, so that within a few generations the organism is almost completely altered. At the same time, scientists are also beginning to synthesize new organisms from scratch. These new technologies hold much promise for improving lives. Genome editing has already been used clinically to treat AIDS patients, by genetically modifying their white blood cells to be resistant to HIV. In agriculture, genome editing could be used to engineer species with increased food output, and the ability to thrive in challenging climates. New bacterial forms may be used to generate energy. But these powerful new techniques also raise important ethical dilemmas and potential dangers, pressing issues that are already upon us given the speed of scientific developments. To what extent should parents be able to manipulate the genetics of their offspring - and would designer babies be limited to the rich? Can we effectively weigh up the risks from introducing synthetic lifeforms into complex ecosystems? In this extensively revised paperback edition, John Parrington explains the nature and possibilities of these new scientific developments, which could usher in a brave, new world. We must rapidly come to understand its implications if we are to direct its huge potential to the good of humanity and the planet.

Jobst Conrad prasentiert die Ergebnisse einer sozialwissenschaftlichen Begleitstudie, die den Prozess und die Chancen der Umsetzung des InnoRegio-Programms des BMBF in der Praxis untersucht. Daruber hinaus gibt er einen Uberblick uber Innovations- und Netzwerkkonzepte, Innovationsdynamik und Technologiepolitik sowie uber Geschichte und Entwicklungstrends der Biotechnologie, Biotechnologiepolitik und Gentechnikdiskurs.

Entwicklungen in Biomedizin, Genetik und Informatik fA1/4hren zu einem Wissenszuwachs im VerstAndnis der Ursachen und Entstehungsmechanismen von Krankheiten. Damit verbunden ist die Hoffnung, zukA1/4nftig Krankheitsrisiken frA1/4hzeitiger zu erkennen und durch MaAnahmen der Entwicklung von Krankheiten vorzubeugen. Mehr als andere Untersuchungsergebnisse erlauben genetische Informationen Aussagen A1/4ber den Gesundheitszustand auch von FamilienangehArigen. Sie bergen damit das Risiko konfligierender Interessen im Hinblick auf deren autonome LebensfA1/4hrung und informationelle Selbstbestimmung in sich.

Das Buch enthAlt die auf dem 10. Einbecker Workshop gehaltenen VortrAge, die sich aus medizinisch-naturwissenschaftlicher und juristischer Sicht mit dem Thema befassen. Im Anhang werden wichtige Materialien zum Thema Gentest ergAnzt. So ist das Asterreichische Gentechnikgesetz, der Schweizer Entwurf eines Gentestgesetzes und der Entwurf der Fraktion der GRAoeNEN im Bundestag fA1/4r ein Deutsches Gentestgesetz enthalten.

One of the biggest challenges faced in medical research had been to create accurate and relevant models of human disease. A number of good animal models have been developed to understand the pathophysiology. However, not all of them reflect the human disorder, a classic case being Usher's syndrome where the mutant mice do not have the same visual and auditory defects that patients face. There are others which have been even more difficult to model due to the multi-factorial nature of the condition and due to lack of discovery of a single causative gene such as age-related macular degeneration or Alzheimer's syndrome. Thus a more relevant and accurate system will allow us to make better predictions on relevant therapeutic approaches. The discovery of human pluripotent stem cells in 1998 followed by the technological advances to reprogram somatic cells to pluripotent-stem cell-like cells in 2006 has completely revolutionized the way we can now think about modelling human development and disease. This now coupled with genome editing technologies such as TALENS and CRISPRs have now set us up to develop in vitro models both 2D as well as 3D organoids, which can more precisely reflect the disease in the patients. These combinatorial technologies are already providing us with better tools and therapeutics in drug discovery or gene therapy. This book summarizes both the technological advances in the field of generation of patient specific lines as well as various gene editing approaches followed by its applicability in various systems. The book will serve as a reference for the current state of the field as it: -Provides a comprehensive overview of the status of the field of patients derived induced pluripotent stem cells. -Describes the use of cardiac cells as a main featured component within the book. -Examines drug toxicity analysis as a working example throughout the book.

Der Autor erklart die Widerstande gegen einzelne Anwendungsbereiche der Gentechnik, beleuchtet deren historische Hintergrunde und untersucht Moglichkeiten, die Akzeptanz der Gentechnik zu beeinflussen."