Human teratocarcinoma has long held a fascination to the pathologist be- cause of the diversity of the seemingly benign differentiated tissues asso- ciated with the tumor. An understanding of this diversity came with the demonstration of the pluripotent nature of the embryonal carcinoma cell, the stem cell of the tumor, in experimental animals. Single cell clones of murine embryonal carcinomas were derived and the resultant cell lines injected into immunosuppressed hosts, where the embryonal carcinoma stem cell itself gave rise to the wealth of differentiated tissues seen in teratocarcinomas. It was also shown that teratocarcinomas could be pro- duced experimentally by transplantation of staged early mouse embryo to extrauterine sites; subsequent experiments suggested that the embryonal carcinoma stem cell was, in fact, comparable to a normal cell of the em- bryonic ectoderm. Many elegant experiments have since been built on this early work; murine embryonal carcinoma cells are providing the ex- perimental material for a first biochemical evaluation of the earliest dif- ferentiation events in mammalian embryogenesis. In this volume, we relate the beginnings of experimental research on human teratoma. The human embryonal carcinoma cells have been char- acterized and established in vitro, providing an experimental model to study the unique biology of these cells. Monoclonal antibodies, estab- lished from work with these in vitro cultures, provide cell surface probes that define populations of embryonal cells and their derivatives in tumor sections.

T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo, and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.

The localized attachment of circulating leukocytes to endothelium has been recognized as the cellular hallmark of the inflammatory response. This adhesive interaction, a necessary antecedent to the emigration of leukocytes from the blood into the tissues, is mediated by vascular adhesion molecules. Leukocyte Recruitment, Endothelial Cell Adhesion Molecules and Transcriptional Control: Insights for Drug Discovery outlines some of the cellular and molecular mechanisms of inflammation with contributions from top researchers. This volume provides an overview of three of these endothelial adhesion molecules, as examples of key mediators of leukocyte recruitment. It reviews the structure and regulation of these cell surface proteins and focus on the rapidly expanding field of transcriptional regulation of these inducible proteins, and closes with a discussion of drug discovery possibilities that target the regulation of leukocyte recruitment. This book will be of interest for any researchers, in academia or industry, looking for an overview of leukocyte recruitment or novel approaches to drug discovery.

Towards the end of this century we celebrate three great discoveries of the last one - of X-rays in 1895, of radioactivity in 1896 and of radium in 1898 - and recall the pioneering achievements that founded the new science of radiology and changed the face of medicine forever. Dr Mould's comprehensive centennial history makes a unique contribution to the telling of this entertaining story - in the unusual and accessible style of a 'radiological photograph album'. Over 700 historical illustrations, with full and informative captions, are supported by short introductory essays to blow the dust off our fascinating radiological past in an easily readable style. The focus of this book is on the historically more interesting earlier years - of discovery and invention, diagnosis and therapy, dosimetry, risk and protection. The photographic record is complemented by archival accounts of the pioneer scientists and physicians, and of their early patients, and is interspersed with a variety of radiological anecdotes. In the several chapters on diagnostic techniques, radiotherapy and nuclear medicine the history is nevertheless brought up to date so that the old methodologies may be contrasted with newer technologies. The medical theme is itself complemented by two interesting chapters on museum and industrial applications of radiography. The predominantly photographic presentation of this book is derived from the author's earlier but smaller A History of X-rays and Radium, no longer available yet still of enduring interest. Research has been based on original source material which is fully cited and the illustrations and text are comprehensively indexed for easy retrieval of the wide variety ofpeople, techniques, apparatus and examples featured throughout this radiological journey.

Pocket Guide to Gene Level Diagnostics in Clinical Practice is an abbreviated, pocket-size, quick-reference guide that provides a point-by-point synopsis of the vast wealth of information contained in CRC Handbook of Gene Level Diagnostics in Clinical Practice. All sections and subsections in the Pocket Guide are cross-referenced to corresponding pages in the Handbook. The book works well on its own as a quick reference, but also can be used in conjunction with the larger Handbook for detailed coverage and references to specific information. Pocket Guide to Gene Level Diagnostics in Clinical Practice also includes extensive supplements featuring material not included in the Handbook. These are intended to provide an up-dated, practical source of information useful to anyone involved in molecular diagnostic research and/or service. Supplements are cross-referenced to the main text of the Pocket Guide, that complement and enhance the material covered. Pocket Guide to Gene Level Diagnostics in Clinical Practice will be a handy reference for professionals and students in pathology, biotechnology, biology, and medicine.

Over the last decade, interest in mycoplasmas has been greatly sti- lated by the spread of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides SC, in Europe and Africa and the discovery of a possible association between mycoplasmas and AIDS. During this period there has also been a recognition by control authorities and int- national organizations, including the Office International des Epizooties, that mycoplasmas are major causes of economic loss in livestock and continue to be a problem in humans as a result of a range of chronic respiratory and urogenital diseases. However, it is likely that the true significance of my- plasmas is still underestimated because of the difficulties in working with these extremely fastidious microorganisms. Advances in such laboratory technology as DNA amplification has enabled the detection of unrecoverable mycoplasmas in tissue samples and this will provide us with a better understanding of their role in disease. Important developments have also been seen in more conv- tional areas such as serological tests and media formulation enabling improved recovery and identification. Mycoplasma Protocols provides in a single volume up-to-date and easy-- follow methods for the detection, isolation, identification, and characterization of mycoplasmas, with a major emphasis on those of medical and veterinary significance. It also includes biochemical, genetic, and molecular techniques that will form the basis of understanding pathogenicity and, particularly, - hesion to host cells.

The Number One bestselling memoir from Ireland's former state pathologist In 1997, Dr Marie Cassidy arrived in Dublin from Glasgow. There to discuss a possible deputy state pathologist post with Professor John Harbison, instead she was whisked by police escort to a Grangegorman murder scene. There was no turning back. She became Ireland's State Pathologist from 2004 until 2018, her image synonymous with breaking news of high-profile cases - a trusted figure in turbulent times. Here, with the scalpel-like precision and calm authority of her trade, Marie shares her remarkable personal journey from working-class Scotland into the world of forensic pathology, describing in candid detail the intricate processes central to solving modern crime. She recounts her work following the tragic deaths of Rachel O'Reilly, Siobhan Kearney, Robert Holohan, Tom O'Gorman and others - along with the Stardust exhumations and lesser known cases from her long career - outlining the subtle methods by which pathology and the justice system meet. Beyond the Tape is a unique behind-the-scenes journey into the mysteries of unexplained and sudden death - by turns poignant, stark and deeply compelling.

This volume provides a history of Eosinophilic Esophagitis (EoE), a basic understanding of the physiology of the eosinophil, and a current understanding of the pathophysiology and genetics of EoE. The emphasis is on clinical applications including presenting symptoms, diagnosis and treatment options for patients with EoE. Written by both pediatric and adult experts in the fields of gastroenterology, allergy and pathology, this volume includes the most up to date information. Providing practical information useful in the treatment of patients, this book will be of great value to gastroenterologists, allergists, pathologists, medical residents, fellows, internists, and general practitioners who treat patients with eosinophilic esophagitis.

General Pathology covers the study of pathological or disease processes in general with particular reference to morphological changes. The book is designed for second- and third-year pathology students in the medical and paramedical fields. Topics covered include cell injury, death and necrosis, pigmentation, calcification, haemorrhage, shock and oedema.

Crime scene reconstruction (CSR) is today's hot topic. The immense proliferation of television, print, and electronic media directed at this area has generated significant public interest, albeit occasionally encouraging inaccurate perceptions. Practical Crime Scene Analysis and Reconstruction bridges the gap between perception and reality, helping readers understand the nature of the scientific method and teaching the proper application of its components. A proven methodology The book begins by recounting the history of crime scene analysis and exploring fundamental principles. Then the authors introduce their proven methodology, known as Event Analysis. This technique defines specific actions, discusses the order of those actions, and offers significant insight into determining what did or did not happen in the course of the incident under investigation. Using case studies and more than 200 color photos, the book demonstrates this method and how it can be used to explain clues that would otherwise be puzzling or ambiguous. Practical advice from the crime scene to the courtroom The authors show how to resolve significant questions that arise in the course of CSR through the use of an event analysis worksheet. The book also discusses crime scene protocol, bloodstain pattern analysis, gunshot investigation, and forensic pathology and the human body. The final chapters provide instruction on writing crime scene reports, discuss ethical issues, and give advice on courtroom presentation. Includes color photos demonstrating the science of CSR on: Superposition Timing and sequence Bloodstain analysis Ballistics Wound patterns

Offering rapid, portable access to key concepts and principles of pathology from Robbins and Cotran Pathologic Basis of Disease, 10th Edition, this up-to-date Pocket Companion makes it easy to locate essential information on the go. The condensed, at-a-glance format, organized to parallel the parent text, is ideal for quick review-anytime, anywhere. Features cutting-edge information on important topics such as novel therapies for hepatitis C, personalized medicine, the role of microbiome and metabolome in non-communicable disease, and much more. Includes new gross and microscopic figures for clarity of morphology and new artwork depicting the latest advances in molecular pathogenesis of cancers. Reflects updated page references and content changes found in to Robbins and Cotran Pathologic Basis of Disease, 10th Edition. Contains all the key data and principles needed for the USMLE Step 1, in-course exams, and rotations. Enhanced eBook version included with purchase. Your enhanced eBook allows you to access all of the text, figures, and references from the book on a variety of devices.

Immunometabolism has emerged as an intersectional crossroad between metabolism and immune response. Over the past decade, it has become clear that most - if not all - immune cell functions are not separated from cellular metabolism. Although seminal works have addressed the metabolic fate of immune cells during differentiation and function, the physiological status of a given tissue is also dependent on the cell metabolism. The dialogue between immune cells and their microenvironment can also modulate cellular metabolism, which can trigger the onset and progression of a multitude of inflammation-mediated diseases. Thus, uncovering the specific characteristics of the metabolism in different immune cells types and in different conditions, can shed light into the molecular mechanisms of disease and help develop new drugs and therapeutic strategies to treat immune diseases. The edited volume Essentials aspects of Immunometabolism will give the readers a broad view on how metabolic pathways can influence many types of immune cells during activation, differentiation and function, in health and disease. Of note, the structure of the book was created thinking not only on the experienced immunologist but also on undergraduate and graduate students, physicians, and all members of the scientific community interested in this exciting field of research.

A complete and balanced overview of all aspects of immunocytochemistry is presented providing a clear understanding of their impact on experiment. All available techniques and many diagnostic and research applications are included, as well as practical step-by-step instructions for carrying out recommended methods. Intended for the novice as well as the experienced researchers.

This expert volume in the Diagnostic Pathology series is an excellent point-of-care resource for practitioners at all levels of experience and training. Covering all areas of nonneoplastic pediatric pathology, it incorporates the most recent clinical, pathologic, and molecular knowledge in this challenging field to provide a comprehensive overview of all key issues relevant to today's practice. Richly illustrated and easy to use, Diagnostic Pathology: Nonneoplastic Pediatrics is a one-stop reference for accurate, complete pathology reports, ideal as a day-to-day reference for both pediatric and general pathologists or as a reliable training resource. Provides a clear framework for a better understanding of the clinical and histopathologic appearances of more than 270 nonneoplastic entities, covering every organ system with a focus on reactive, infectious, congenital, and genetic conditions Covers significant advances in this complex field, including recent molecular discoveries and updated disease entities, new information on multiple immunologic disorders, changes and expansions to several classification systems (such as WHO, Banff, and ISSVA), and updates in other scoring systems Contains new chapters on manifestations of pediatric COVID-19 infection, tumoral calcinosis, multiple skeletal dysplasia, skeletal muscle, collagenous gastritis and colitis, adenovirus appendicitis, intestinal pseudoobstruction, disorders of sexual development, and Kikuchi disease Includes approximately 3,000 high-quality clinical and histologic images, gross pathology images, radiologic images, and full-color illustrations to help practicing and in-training pathologists reach a confident diagnosis-as well as nearly 200 additional images online Employs consistently templated chapters and bulleted content for quick, expert reference at the point of care Includes an eBook version that enables you to access all text, figures, and references, with the ability to search, customize your content, make notes and highlights, and have content read aloud

This brief, accessible treatise harnesses the neurophysiological processes of learning to create an innovative and powerful approach to therapy. It sets out a non-pathologizing alternative not only to the current medicalized conception of diagnosis and treatment but also to the labeling of relatively normal reactions to stressors and upsets as illnesses. Rooted in the neurobiology of human learning, the book's approach to treatment, Neuro-Cognitive Learning Therapy, characterizes maladaptive behavior patterns as learned responses to upsetting conditions-processes which can be unlearned. In addition, the coverage includes a clinical teaching guide for bringing NCLT theory and methods into the training curriculum. This groundbreaking volume: Proposes a non-stigmatizing learning model for therapy, Neuro-Cognitive Learning Therapy. Introduces the concept of the connectome and explains its critical role in mental health and illness. Differentiates between the unconscious and automaticity in cognition and behavior. Addresses the applicability of NCLT to biologically-based mental disorders. Offers case studies illustrating NCLT in contrast with commonly-used approaches. Includes a chapter-by-chapter clinical teaching guide with therapeutic principles and discussion questions. Provides a comprehensive therapeutic framework for practitioners of all orientations. Depathologizing Psychopathology gives neuropsychologists, psychiatrists, clinical social workers, and child and school psychologists new ways of thinking about mental illness and learning about learning for a bold new step in the evolution of mind/brain knowledge.

This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.

This detailed volume presents a variety of animal models that are commonly used to study sepsis and some key procedures to measure specific disease outcomes. The chapters describe well-established surgical and nonsurgical rodent models of sepsis, presented by experts in the field. In addition, the book includes protocols for burn injury and sepsis, modeling the mouse intensive care unit (MICU), and the development of humanized mice, which may be useful tools to increase the translational potential of rodent sepsis research. There is a chapter discussing the use of biomarkers for sepsis diagnosis and prognosis in humans and mice, as well as chapters describing specific methods for biomarker measurement, and the use of analgesics and humane endpoints in rodent sepsis research. Finally, agent-based computational modeling is presented as a valuable complementary approach to study sepsis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Sepsis: Methods and Protocols serves as an ideal guide for researchers working toward improving the identification and treatment of septic patients.

The purpose of the book is to bring the two disciplines - vascular mechanics and pathology - together. In addition, the book bridges the gap in our knowledge and enhances engineering applications in medicine. This cutting-edge work presents the use of veins as arterial grafts and discusses the role of vein valves in graft stenosis. The book illustrates aneurysm formation, growth, and rupture, using pressure vessel principles. This new work details the investigation of, amongst other topics, aortic dissection, showing for the first time that the aortic root mechanics plays a vital role in the development of this pathology.

The definitive interdisciplinary reference work for wound ballistics Basics The book begins by providing the necessary basic knowledge about physics, ballistics and ammunition and weapons. Then it describes the behaviour of projectiles in humans and animals (the physics of gunshot injuries) and introduces the experimental simulation of gunshot wounds, including the materials suitable for this purpose. Applications These basic principles can be applied in forensic medicine and criminalistics. The gunshot wound provides some forensic traces and the experimental reconstruction helps to understand the dynamic crime process. The wounding potential of non-lethal weapons can be determined. In emergency and war surgery, injuries caused by small arms bullets and fragments as well as by gas jets (of gas weapons) can be assessed. International conventions could be freed from undefined terms (such as "unnecessary suffering") with the help of physical quantities. Reference work Detailed tables as e. g. ballistic data of numerous cartridge types, also older ones, material properties, as well as many otherwise difficult to access data and a trilingual glossary of ballistic and technical terms in the languages German, English and French. NEW Wound Ballistics of European Police ammunition References to recent research results Partly coloured illustrations Due to the increase in terrorist and criminal activities worldwide, it is not only members of the armed forces who are affected. Surgeons, forensic doctors, police officers and criminalists also need to know and be able to assess the specifics of gunshot wounds.

THE TRUE CRIME BOOK OF THE YEAR AND SUNDAY TIMES TOP 10 BESTSELLER 'One of the most fascinating books I have read in a long time. Engrossing, a haunting page-turner. A book I could not put down' The Times, BOOKS OF THE YEAR __________ Meet the forensic pathologist, Dr Richard Shepherd. He solves the mysteries of unexplained or sudden death. He has performed over 23,000 autopsies, including some of the most high-profile cases of recent times; the Hungerford Massacre, the Princess Diana inquiry, and 9/11. He has faced serial killers, natural disaster, 'perfect murders' and freak accidents. His evidence has put killers behind bars, freed the innocent, and turned open-and-shut cases on their heads. Yet all this has come at a huge personal cost. Unnatural Causes tells the story of not only the cases and bodies that have haunted him the most, but also how to live a life steeped in death. Thoughtful, revealing, chilling and always unputdownable, if you liked All That Remains, War Doctor and This is Going to Hurt you'll love this. **Pre-order Dr Richard Shepherd's new book THE SEVEN AGES OF DEATH now** __________ 'Gripping, grimly fascinating, and I suspect I'll read it at least twice' Evening Standard 'A deeply mesmerising memoir of forensic pathology. Human and fascinating' Nigella Lawson 'An absolutely brilliant book. I really recommend it, I don't often say that but it's fascinating' Jeremy Vine, BBC Radio 2 'Puts the reader at his elbow as he wields the scalpel' Guardian 'Fascinating, gruesome yet engrossing' Richard and Judy, Daily Express 'Fascinating, insightful, candid, compassionate' Observer

This book takes Complexity Theory and applies it to medicine where it has previously made little ground. It provides new hypotheses for multiple common but misunderstood diseases. Doctors in particular will understand that many diseases have remained unsolved due to a linear approach to what are complex biological systems, and failure to understand and apply Complexity Theory. Therefore, many common conditions have no known cause and consequently treatments are either ineffectual or non-existant, when many of these diseases are in fact preventable. There is growing interest in non-linear science, dynamic systems, chaos and complexity theory. This trend has directly involved other sciences, including biology, but has been little touched on by medicine. Readers of this book will: * Understand the difference between Linear Science and Complexity Theory, and how medicine has failed to apply the latter. * Recognise the advantages of using this understanding to generate realistic hypotheses for cause of disease. * Read how hypotheses so generated have been formulated for a number of common diseases.

Blood Science The second edition of the leading introduction to blood science, with updated new illustrations and case studies Blood Science: Principles and Pathology integrates hematology and blood transfusion, -clinical biochemistry, and immunology to provide a thorough introduction to this rapidly expanding discipline. Reflecting recent changes in education and training for healthcare scientists, this comprehensive textbook covers the analytical techniques used in blood -science, the diagnosis and management of various blood disorders, and more. Fully revised, the second edition presents new case studies and high-quality images -throughout, illustrating the practical skills and knowledge required by today's under-graduate students and practitioners. Detailed yet accessible chapters contain learning objectives and summaries, links to further readings and resources, and real-world case studies with easy-to-follow interpretations. Throughout the text, the authors highlight how laboratory data and clinical details are used to investigate patients with actual or suspected diseases in real-world scenarios: Multi-disciplinary view merging biochemical, hematological, immunological, and genetical knowledge into a single discipline: Blood science Discusses advances in molecular genetics identifying mutations resulting in the occurrence of certain pathological conditions such as leukaemia Presents an expanded concluding chapter with detailed case reports that integrate biochemistry, immunology, and haematology, which all contribute to the investigation of respective conditions Explains the potentials for developing tests such as non-coding RNAs Offers further reading suggestions to dive even deeper into discussed subjects and concepts Designed to meet the needs of undergraduate students taking blood science modules in biomedical, biological, and healthcare science programs, Blood Science: Principles and Pathology, Second Edition is also an invaluable guide for new graduates entering the field, as well as those training for professional qualifications or working with blood samples in laboratory-based environments.