This book presents new insights into the etiology and pathogenesis of systemic diseases recently discovered to be due to specific defects in molecular assemblies, organelles, or other subcellular structures. This new information, of great importance to medical students, physicians, and basic scientists, has not been incorporated into major medical textbooks. The volume will be an important resource for medical or graduate students, investigators and physicians, as it covers major new insights into diverse diseases and concepts missing from pathology textbooks. Each of the ten chapters in the book is written by a leader in the field.

Through five well-regarded editions, Dr. David Dabbs' Diagnostic Immunohistochemistry has set the standard for concise, complete, guidance on the use and interpretation of immunohistochemical stains. The 6th Edition continues this tradition of excellence, bringing you fully up to date with all aspects of this dynamic field. Easy to use and understand, this practical resource distills the large body of information on immunohistochemistry into a single, convenient reference that is invaluable for today's surgical pathologists. Covers all aspects of the field, with an emphasis on the role of genomics in diagnosis and theranostic applications that will better inform treatment options. Includes the latest grading schemes in several organs along with new antibodies to cover more genomic immunohistochemistry applications. Contains current biomarker guidelines and up-to-date references throughout. Offers a systematic approach to the diagnostic entities of each organ system, including detailed differential diagnoses, diagnostic algorithms, and immunohistograms that depict immunostaining patterns of tumors. Contains numerous charts and tables, as well as 1,500 high-quality color histologic images that assist in making a definitive diagnosis. Discusses diagnostic pitfalls through immunohistologic differential diagnosis wherever appropriate so you can provide the most accurate diagnoses. Covers many more antigens than other texts, and discusses antibody specifications with tables that convey information on uses, clones, vendors, sources, antibody titers, and types of antigen retrieval. Enhanced eBook version included with purchase. Your enhanced eBook allows you to access all of the text, figures, and references from the book on a variety of devices.

This inaugural issue of Advances in Molecular Pathology will provide a comprehensive review of the most current practices, trends, and developments in the field of Molecular Pathology. Publishing on an annual basis, the volume will be divided into 7 sections: Genetics, Hematopathology, Infectious Disease, Pharmacogenomics, Informatics, Solid tumors, and Identity/HLA. Led by Dr. Gregory Tsongalis of Dartmouth University, a team of experienced pathologists from institutions across the country oversee annual topic and expert author selection. Topics discussed in this volume include, but are not limited to: whole genome sequencing in critically ill children, bioinformatics in clinical genomic sequencing, comprehensive monitoring of patients with chronic myeloid leukemia, genetic biomarkers in the biology and clinical workup of chronic lymphocytic leukemia, metagenomics in infectious disease, point of care molecular testing, pharmacogenomics in oncology, clinical uses of panel testing vs. single gene testing, large scale data sharing initiatives in genomic oncology, clinical NGS assays for solid tumors emerging concepts in liquid biopsy the cell line and tissue misidentification problem, and cell line detective work.

This issue of Medical Clinics, guest edited by Dr. Cheston B. Cunha, is devoted to Antimicrobial Stewardship. Articles in this issue include: Principles of Antimicrobial Stewardship; Antibiotic Resistance in Stewardship; Therapy of Resistant Organisms: A Stewardship Approach; Optimal Antibiotic Dosing Strategies; The Importance of Interdisciplinary Collaboration in Antimicrobial Stewardship; Role of Education in Antimicrobial Stewardship; Role of the Hospital Epidemiologist in Antimicrobial Stewardship; Role of the Clinical Microbiology Laboratory in Antimicrobial Stewardship; Role of New and Rapid Diagnostics In Antimicrobial Stewardship; Antimicrobial Stewardship in the Community Hospital; Antimicrobial Stewardship in Long-Term Care Facilities; Role of the Pharmacist in Antimicrobial Stewardship; Pharmacoeconomic Considerations of Antimicrobial Stewardship Programs; Principles of IV-to-PO Switch and PO therapy; Role of Technology in Antimicrobial Stewardship; and Metrics of Antimicrobial Stewardship Programs.

Combined fields of Microbiology and Nanotechnology have been most successful in providing novel solutions for protecting the health of humans and environment. This book covers the implications of nano-strategies to combat bacterial pathogens, applications of nanotechniques in microbiology, and innovative advances in the area of medical microbiology. Contents are divided into three sections -- Nanoscience in controlling bacterial pathogens, Nanoscience in Microbiology, Medical Microbiology. This volume is going to provide timely information about the technological advances of Nanoscience in the domain of Microbiology, with a special emphasis on Pathobiology. The book is a useful read for students and researchers in microbiology, nanotechnology and medical microbiology.

This issue of Clinics in Laboratory Medicine will focus on Clinical Pathology and is edited by Geza S. Bodor. Topics include, but are not limited to, Steroid measurement / Salivary cortisol measurement, Protein testing by LCMSMS, LCMSMS in the Clinical Laboratory, Laboratory Standards for Clinical LCMSMS, The need to teach LCMSMS to clinical laboratory scientists, MALDI-TOF in the clinical laboratory, MALDI TOF MS in the clinical microbiology laboratory, LCMSMS method development consideration in clinical laboratory practice, Cancer diagnosis using mass spectrometry, Adulteration and LCMSMS drug testing, Diagnosis of inherited metabolic disorders using LCMSMS, Harmonization of LCMSMS protein assays, Vitamin D testing by LCMSMS versus by immunoassay, Pain management testing by LCMSMS, and Development of FDA approved clinical mass spectrometer.

This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab. Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and the novel coronavirus disease that results from it.

This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.

Biomolecular Free Radical Toxicity: Causes and Prevention provides a comprehensive overview of biomolecular injury. By discussing recent research and providing interpretations of the available data, this unique and timely book explores the causes of biomolecular injury and the possible routes to its prevention. Split into three sections, the book covers:
* macromolecular mechanisms of injury and the role of antioxidants;
* gene expression and cell mediated mechanisms of toxicity expression; and,
* human and environmental health effects.
Written by experts in the field, Biomolecular Free Radical Toxicity: Causes and Prevention will be invaluable to researchers specialising in food and nutrition-based toxicology, biomolecular sciences, biochemistry, genetics, environmental biomonitoring, drug metabolism and carcinogenesis.

This issue of Surgical Pathology Clinics, edited by Dr. John Hart, focuses on Liver Pathology. Topics include--but are not limited to--Hereditary cholestatic disorders; Autoimmunity in the liver; Fibrolamellar carcinoma; Hepatocellular adenoma; Infections/granulomas in the liver; HCV and direct acting antivirals; Lymphomas in the liver; Viral hepatitis; NASH; Drug induced liver injury; PBC and AIH; Liver transplant; Frozen sections of the liver; Hepatocellular carcinoma; and Cholangiocarcinoma.

The accurate and reliable diagnosis of transmissible diseases is the most powerful weapon available to ensure their control, and in some cases eradication. The detection of parasites in clinical cases, companion and farm animals, and in the environment is relatively easy since many of them are visible to the naked eye, and those that are not are readily detected by light microscopy. Fungal infections can similarly be determined. Bacteria are somewhat harder to detect. Although their presence can frequently be detected by light microscopy, differential diagnosis, beyond their gross morphology, is almost always impossible. However, most bacterial pathogens can be cultured in the laboratory and can be accurately identified by combinations of a series of simple tests such as morphology, staining, antibiotic sensitivity, biochemical analyses, nutrient dependence, and phage sensitivity. Viruses, however, have proved much more difficult; their size and absolute dependence of the host cell for propagation have rendered useless the methods traditionally used for other microorganisms. Until the development of tissue culture in the middle of this century, diagnosis was entirely dependent on the skill and experience of the clinician. But this was an unreliable process since many of the common virus infections exhibit similar clinical symptoms, such as coryza, exanthema, vomiting, diarrhea, neuralgia, and lethargy. Indeed many viral infections display clinical signs that are indistinguishable from bacterial or parasitic infections.

Over the last ten years, much effort has been devoted to improving the biophysical techniques used in the study of viruses. This has resulted in the visualization of these large macromolecular assemblages at atomic level, thus providing the platform for functional interpretation and therapeutic design. Structural Virology covers a wide range of topics and is split into three sections. The first discusses the vast biophysical methodologies used in structural virology, including sample production and purification, confocal microscopy, mass spectrometry, negative-stain and cryo-electron microscopy, X-ray crystallography and nuclear magnetic resonance spectroscopy. The second discusses the role of virus capsid protein structures in determining the functional roles required for receptor recognition, cellular entry, capsid assembly, genome packaging and mechanisms of host immune system evasion. The last section discusses therapeutic strategies based on virus protein structures, including the design of antiviral drugs and the development of viral capsids as vehicles for foreign gene delivery. Each topic covered will begin with a review of the current literature followed by a more detailed discussion of experimental procedures, a step in the viral life cycle, or strategies for therapeutic development. With contributions from experts in the field of structural biology and virology this exceptional monograph will appeal to biomedical scientists involved in basic and /or applied research on viruses. It also provides up-to-date reference material for students entering the field of structural virology as well as scientists already familiar with the area.

Molecular Pathology and the Dynamics of Disease bridges the basic science of, and primary clinical literature on, human disease. Topics covered include several major disease areas, such as inflammation and host response, vascular disease, obesity, weight regulation and appetite, cancer biology, drug development, and gene- and cell-based therapeutics that are all presented in a way that emphasizes the interplay between clinical care and investigation. As new technologies and techniques are constantly changing and laboratory scientists plays a critical role in validating data used by clinicians in diagnosing patients, this book provides a timely guide that includes a clinical, research and theory perspective.

Highly illustrated and conveniently templated for quick reference, Atlas of Liver Pathology, 4th Edition, is a useful Atlas and text for every practicing pathologist or trainee who assesses liver biopsy specimens. From cover to cover, it contains all the information needed to identify histologic features and correlate them with clinical findings, offering a clearer understanding of the clinical implications of the disease as it relates to treatment. More than 1,200 high-quality, full-color images and illustrations provide you with a complete visual guide to the vast majority of liver diseases and assist in the diagnoses of biopsy and resected liver specimens. Provides clear, templated information for each disease: Major Morphologic Features; Special Stains; Differential Diagnoses; Clinical and Biologic Behavior; and Treatment and Prognosis.  Incorporates relevant data from ancillary techniques (immunohistochemistry, cytology, cytogenetics and molecular genetics), giving you the necessary tools required to master the latest breakthroughs in diagnostic technology.  Incorporates the latest diagnostic biomarkers and their utility in differential diagnoses, newly described variants, and new histologic entities.  Contains two new chapters on liver biopsy interpretation and autoimmune hepatitis.  Features concise, bulleted text and abundant tables that cover common as well as rare diseases, differential diagnoses, and more.  An eBook version is included with purchase. The eBook allows you to access all of the text, tables, figures and references, with the ability to search, customize your content, make notes and highlights, and have content read aloud. 

Studies on Hepatitis Viruses: Life Cycle, Structure, Functions, and Inhibition presents the latest on this systemic infection that predominantly affects the liver with inflammation that can be acute or chronic. Hepatitis viruses have been the subject of intense study in the last twenty years, with a wealth of information related to their lifecycle, structure, functions and inhibition being presented. This book compiles the most important developments and research, giving users a very useful guide on this evolving area of virology and medicinal chemistry.

Transfusion Medicine for Pathologists: A Comprehensive Review for Board Preparation, Certification, and Clinical Practice is a concise study guide designed to complement standard textbooks in the field of clinical pathology. Pathology residents and fellows of transfusion medicine will find this book useful as a preparation tool for their exams. In addition, the book is a valuable timesaver for busy residents looking for a focused and compact study guide on transfusion medicine that will also be ideal for practicing pathologists who cross-cover transfusion medicine in their clinical practice.

Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.

This issue of Surgical Pathology Clinics, edited by Dr. Laura C. Collins, will focus on Breast Pathology. Topics include, but are not limited to: Core needle biopsy of the breast, Mucinous Lesions of the Breast: Pragmatic approach to fibroepithelial lesions; Lymph node inclusions; Differential diagnosis of benign spindle cell lesions of the breast; Ancillary Prognostic and Predictive Testing in Breast Cancer; Lesions of the Low Grade Breast Neoplasia Pathway; Genotype-Phenotype Correlation in Breast Neoplasia; Lobular Carcinoma in Situ; and Processing and Reporting of Breast Specimens in the Neoadjuvant Setting.

This updated issue of Clinics in Laboratory Medicine, edited by Martin H. Bluth, will focus on Molecular Pathology. Topics include, but are not limited to, The Impact of Molecular Pathology on the Practice of Pathology; Molecular pathology techniques; Clinical Implication of MicroRNAs in Molecular Pathology; Diagnostic Molecular Microbiology; Molecular Pathology in Transfusion Medicine; Molecular Diagnosis of Hematopoietic Neoplasms; Molecular Diagnostics in Colorectal Carcinoma; Molecular Diagnostics in the Neoplasms of Small Intestine and Appendix; Molecular Diagnostics in Esophageal and Gastric Neoplasms; Molecular Diagnostics in the Neoplasms of the Pancreas, Liver, Gall Bladder, and Extrahepatic Biliary Tract; Current Applications of Molecular Genetic Technologies to the Diagnosis and Treatment of Cutaneous Melanocytic Neoplasms; Breast Carcinoma; and Gynecologic Cancers.

Chikungunya and Zika Viruses: Global Emerging Health Threats is the go-to resource for both historical and current information on this important virus that is rapidly increasing its global range. Epidemics since 2005 have spread from Africa and Asia, and through Europe, and an ongoing epidemic has caused nearly two million cases in the Americas. It causes severe crippling arthritis, with symptoms lasting for months or years. As no vaccine or treatment is available, there is international interest in the virus, thus funding opportunities for research have dramatically increased. This book presents our understanding of the virus, bringing comprehensive knowledge in a single source.

This reference text is a must have for any current or future clinicians or students of microbiology. It is concisely organized to provide vital information on many of the microbes one will regularly encounter and the most efficacious ways of addressing associated infections. Discussion of antimicrobial resistance mechanisms and measures to combat them are also one of the key features of this text. Whether you desire to utilize this book at the bedside for prompt treatment decisions or as a reference manual to be used at your leisure, you will find it to be a valuable addition to your library.

Atlas of Conducted Electrical Weapon Wounds and Forensic Analysis provides a comprehensive publication on the subject of Conducted Electrical Weapon (CEW) wounds and signature markings created by this class of weapon. This volume will serve as a very useful resource for all professions tasked with assisting persons that have allegedly been subjected to a CEW exposure. The volume provides an introduction to basic CEW technology and the types of CEWs currently available. It also serves as a comprehensive pictorial atlas of signature markings that CEW exposures make in the immediate and more remote post-exposure periods. Also, it discusses the ability of forensic specialty examinations of the CEW itself to aid in the determination of whether the alleged CEW exposure is consistent with the objective evidence and the subjective statements. Finally, this text addresses the important and growing area of factitious CEW markings that will be useful for consideration by investigators and litigators. Atlas of Conducted Electrical Weapon Wounds and Forensic Analysis provides an objective atlas of evidence for reference that will benefit those professionals who often must make diagnostic, treatment or legal judgments on these cases including Emergency and Primary-Care Physicians, Medical Examiners, Forensic Pathologists, Coroners, Law Enforcement Investigators, and Attorneys.

Fundamentals of Toxicologic Pathology, Third Edition, presents an essential overview of systems toxicologic pathology in a clear-and-concise manner. Toxicologic pathology integrates toxicology and its interdisciplinary components, including biochemistry, pharmacodynamics and risk assessment to pathology and its related disciplines, such as physiology, microbiology, immunology and molecular biology. This wholly revised and updated edition presents the newest information on the topic, and is an essential reference for advanced students, early career researchers, toxicologic pathologists, pharmaceutical scientists, medical pathologists and clinicians, and anyone involved with drug and device development. The book includes a new section describing the application of toxicologic pathology, such as diagnostic and forensic toxicologic pathology, environmental toxicologic pathology, experimental and industrial toxicologic pathology, and pathology issues in the design of toxicology studies. There are also new chapters on special senses (the eye and ear) and the biochemical and molecular basis of toxicity, among others.

Hepatitis C in Developing Countries: Current and Future Challenges explores the current state of HCV in several countries, including Africa, Asia and South America. It maintains a dedicated focus on the epidemiology, clinical patterns, virologic diversity, coinfections, natural history and progression, complications, and response to standard of care (SOC) pegylated interferon and ribavirin therapy of HCV with recommendations specific to middle and low income countries. Readers will find detailed information on the burden of HCV infection from a global health and economic perspective, along with data from multicenter trials on DAAs that have enrolled patients infected with HCV non-genotype 1.

HIV/AIDS: Oxidative Stress and Dietary Antioxidants provides comprehensive coverage of oxidative stress in HIV/AIDS, focusing on both the pathological process around molecular and cellular metabolism and the complications that can arise due to nutritional imbalance. It provides a pathway for researchers and clinicians to gain an in-depth understanding of the role of oxidative stress, bridging the transdisciplinary divide between virologists, immunologists, physicians, clinical workers, food scientists and nutritionists to advance medical sciences and enable preventative treatment strategies. Very often oxidative stress is a feature of HIV/AIDs or of the treatment of HIV/AIDs. While immunologists, physicians and clinical workers understand the processes in HIV/AIDs, they may be less conversant in the science of nutrition and dietetics. Similarly, nutritionists and dietitians may be less conversant with the detailed clinical background and science of HIV/AIDs.