This book explores the fundamental and practical aspects of supercritical fluid chromatography (SFC) and extraction. It discusses packed columns in SFC; detection in SFC; supercritical fluid chromatography/mass spectroscopy; and evaporative light scattering detection in SFC.

Application of NMR and Molecular Docking in Structure-Based Drug Discovery, by Jaime L. Stark and Robert Powers NMR as a Unique Tool in Assessment and Complex Determination of Weak Protein-Protein Interactions, by Olga Vinogradova and Jun Qin The Use of Residual Dipolar Coupling in Studying Proteins by NMR, by Kang Chen und Nico Tjandra NMR Studies of Metalloproteins, by Hongyan Li and Hongzhe Sun Recent Developments in 15N NMR Relaxation Studies that Probe Protein Backbone Dynamics, by Rieko Ishima Contemporary Methods in Structure Determination of Membrane Proteins by Solution NMR, by Tabussom Qureshi and Natalie K. Goto Protein Structure Determination by Solid-State NMR, by Xin Zhao Dynamic Nuclear Polarization: New Methodology and Applications, by Kong Hung Sze, Qinglin Wu, Ho Sum Tse and Guang Zhu

The 37th Annual Denver Conference on Applications of X-Ray Analysis was held August 1-5, 1988, at the Sheraton Steamboat Resort and Conference Center, Steamboat Springs, Colorado. As usual, alternating with x-ray diffraction, the emphasis this year was x-ray fluorescence, but as has been the pattern for several occasions over the last few years, the Plenary Session did not deal with that subject, specifically. In an attempt to introduce the audience to one of the new developments in x-ray analysis, the title of the session was "High Brilliance Sources/Applications," and dealt exclusively with synchrotron radiation, a topic which has made a very large impact on the x-ray community over the last decade. As the organizer and co-chairman of the Plenary Session (with Paul Predecki), it is my responsibility to report on that session here. The Conference had the privilege of obtaining the services of some of the preeminent practitioners of research using this remarkable x-ray source; they presented the audience with unusually lucid descriptions of the work which has been accomplished in the development and application of the continuous, high intensity, tunable, polarized and collimated x-rays available from no facility other than these specialized storage rings. The opening lecture (and I use that term intentionally) was an enthusiastic description of "What is Synchrotron Radiation?" by Professor Boris Batterman of Cornell University and the Cornell High Energy Synchrotron Sourc(! (CHESS).

This detailed volume provides a comprehensive overview of state-of-the-art metabolomics methods based on mass spectrometry (MS), and their application in food, nutrition, and biomedical research. The chapters assembled herein cover hot topics related to sample preparation, chromatographic and electrophoretic separation, MS-based analysis, as well as data processing and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry for Metabolomics serves as a timely guide for chemists, biochemists, biologists, nutritionists, clinicians, and other experts working in the growing and exciting field of metabolomics.

Introducing the most recent advances in crystallography, nuclear magnetic resonance, molecular modeling techniques, and computational combinatorial chemistry, this unique, interdisciplinary reference explains the application of three-dimensional structural information in the design of pharmaceutical drugs. Furnishing authoritative analyses by world-renowned experts, Structure-Based Drug Design discusses protein structure-based design in optimizing HIV protease inhibitors and details the biochemical, genetic, and clinical data on HIV-1 reverse transcriptase presents recent results on the high-resolution three-dimensional structure of the catalytic core domain of HIV-1 integrase as a foundation for divergent combination therapy focuses on structure-based design strategies for uncovering receptor antagonists to treat inflammatory diseases demonstrates a systematic approach to the design of inhibitory compounds in cancer treatment reviews current knowledge on the Interleukin-1 (IL-1) system and progress in the development of IL-1 modulators describes the influence of structure-based methods in designing capsid-binding inhibitors for relief of the common cold and much more!

This book reviews the latest advances in mass spectrometry (MS) techniques applied to food safety and environment quality, and it discusses the recent improvements in sample preparation and MS platforms for screening of emerging contaminants. Expert contributors discuss the current applications from omics to the screening of emerging contaminants and nanomaterials in food and environmental matrices, and particular attention is given to the opportunities that MS offers for guarantying food security and promoting the sustainable use of ecosystems. Divided into 13 chapters, the book covers topics such as the handling and preparation of food and environmental samples for MS, foodomics, environmental omics, ambient ionization techniques in food and environmental chemistry, and chip-based separation devices coupled to MS. Readers will also find a comprehensive overview of several MS techniques applied to food and environmental chemistry, including elemental, isotopic, chiral, ion mobility, chromatographic and imaging MS. This book will appeal not only to students and researchers, but also to professionals working with MS platforms in food safety and environmental quality. The different advances and promising applications described in this work will be of paramount importance for ensuring food safety and environment health for current and future generations.

The objective of this book is to discuss the current status of research and development of boron-rich solids as sensors, ultra-high temperature ceramics, thermoelectrics, and armor. Novel biological and chemical sensors made of stiff and light-weight boron-rich solids are very exciting and efficient for applications in medical diagnoses, environmental surveillance and the detection of pathogen and biological/chemical terrorism agents. Ultra-high temperature ceramic composites exhibit excellent oxidation and corrosion resistance for hypersonic vehicle applications. Boron-rich solids are also promising candidates for high-temperature thermoelectric conversion. Armor is another very important application of boron-rich solids, since most of them exhibit very high hardness, which makes them perfect candidates with high resistance to ballistic impact. The following topical areas are presented: *Boron-rich solids: science and technology *Synthesis and sintering strategies of boron rich solids *Microcantilever sensors *Screening of the possible boron-based thermoelectric conversion materials; *Ultra-high temperature ZrB2 and HfB2 based composites *Magnetic, transport and high-pressure properties of boron-rich solids *Restrictions of the sensor dimensions for chemical detection *Armor

Practical Thin-Layer Chromatography provides thorough coverage of the principles, practices, and applications of thin-layer chromatography (TLC) for important sample and compound types. This information is directed specifically at workers in the most active scientific fields.

Nanospectroscopy addresses the spectroscopy of very small objects down to single molecules or atoms, or high-resolution spectroscopy performed on regions much smaller than the wavelength of light, revealing their local optical, electronic and chemical properties. This work highlights modern examples where optical nanospectroscopy is exploited in photonics, optical sensing, medicine, or state-of-the-art applications in material, chemical and biological sciences. Examples include the use of nanospectroscopy in such varied fields as quantum emitters, dyes and two-dimensional materials, on solar cells, radiation imaging detectors, biosensors and sensors for explosives, in biomolecular and cancer detection, food science, and cultural heritage studies.

Updated and revised throughout. Second Edition explores the chromatographic methods used for the measurement of drugs, impurities, and excipients in pharmaceutical preparations--such as tablets, ointments, and injectables. Contains a 148-page table listing the chromatographic data of over 1300 drugs and related substances--including sample matrix analyzed, sample handling procedures, column packings, mobile phase, mode of detection, and more.

Martin Stein's thesis describes a novel methodology for natural product discovery. Due to its high degree of reproducibility, robustness and sensitivity, the technique can be utilized to detect even trace amounts of bioactive substances in heterogeneous matrices such as fermentation broths or crude organic extracts. This research is thus relevant for a large number of researchers working in natural product discovery. Applications of this novel NMR-based approach include suitable environmental triggers for the induction of biosynthetic machineries. The author demonstrates the extraordinary value of this approach by the successful isolation of two potent inhibitors of the pharmaceutically relevant proteasome core particle from the insect pathogen photorhabdus luminescens. This thesis has led to a number of publications in high-impact journals.

For many years TLC has suffered from the image of being a low sensitivity, low resolution, non-quantitative teehnique, suitable for chemists, but not a tool for real ehromatographers. Whilst perhaps true in the past this attitude no longer ref1eets the eapabi1ities of modern instrumenta1ized TLC in all its many forms. This volume represents the proceedings of a meeting in Brighton in 1987 whieh formed part of a eontinuing series of one and two day events on TLC organized by the Chromatographie Soeiety either alone or, like this one, in eonjunetion with other learned bodies. These meetings are designed to keep ehromatographers up to date with the latest deve10pments and help promote a more positive image of TLC. lan Wilson November 1987 v CONTENTS The Chromatographic Society xi Contemporary Thin-layer Chromatography: An introduction IoD. Wilson INSTRUMENTATION Quantitative Methods in Thin-layer Chromatography C. F. Poole, S. K. Poole and T. A. Dean 11 Fluorescence Line-narrowing Spectroscopy: A New and Highly Selective Detection Technique for Thin-layer and Liquid Chromatography J. W. Hofstraat, C. Gooijer, U. A. Th. Brinkman and N. H. Velthorst 29 Qualitative and Quantitative Image Analysis of Fluorescence from High Performance Thi. n-layer Chromatography R. M. Belchamber, S. J. Brinkworth, H. Read and J. D. M. Roberts 37 Analytical Rotation Planar Chromatography Sz. Nyiredy, K. Dallenbach-Toelke and O. Sticher 45 Analytical and Preparative Overpressured Layer Chromatography E. Mincsovics and E. Tyihak 57 Anticircular Planar Chromatography: Analytical and Preparative Aspects H. Traitler and A.

Metrology is part of the essential but largely hidden infrastructure of the modern world. This book concentrates on the infrastructure aspects of metrology. It introduces the underlying concepts: International system of units, traceability and uncertainty; and describes the concepts that are implemented to assure the comparability, reliability and quantifiable trust of measurement results. It is shown what benefits the traditional metrological principles have in fields as medicine or in the evaluation of cyber physical systems.

This work represents a sound introduction to the fundamental principles of infrared microspectroscopy (IMS). It describes how IMS is used to solve specific microanalytical problems in a variety of disciplines, including forensic analysis, art conservation, and geological, pharmaceutical and electronics research. The book discusses when and how to use special techniques such as line scanning, 3-dimensional imaging and attenuated total reflection and grazing-angle spectroscopy.

This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.

The rapid growth of the subject since the first edition ten years ago has made it necessary to rewrite the greater part of the book. Except for the introductory portion and the section on Mott scattering, the book has been completely revised. In Chap. 3, sections on polarization violating reflection symmetry, on resonance scattering, and on inelastic processes have been added. Chapter 4 has been rewritten, taking account of the numerous novel results obtained in exchange scattering. Chapter 5 includes the recent discoveries on photoelectron polarization produced by unpolarized radiation with unpolarized targets and on Auger-electron polarization. In Chap. 6, a further discussion of relativistic polarization phenomena has been added to the book. The immense growth of polarization studies with solids and surfaces required an extension and new presentation of Chap. 7. All but one section of Chap. 8 has been rewritten and a detailed treatment of polarization analysis has been included. Again, a nearly comprehensive treatment has been attempted. Even so, substantial selectivity among the wide range of available material has been essential in order to accomplish a compact presentation. The reference list, selected along the same lines as in the first edition, is meant to lead the reader through the literature giving a guide for finding further references. I want to express my indebtedness to a number of people whose help has been invaluable.

This thesis presents results from a combined atomic-resolution Z-contrast and annular bright-field imaging and electron energy loss spectroscopy in the Scanning Transmission Electron Microscopy, as well as first principles studies of the interfaces between crystalline " "" "Si3N4 and amorphous (i) CeO2-x as well as (ii) SiO2 intergranular film (IGF). These interfaces are of a great fundamental and technological interest because they play an important role in the microstructural evolution and mechanical properties of Si3N4 ceramics used in many high temperature and pressure applications. The main contribution of this work is its detailed description of the bonding characteristics of "light" atoms, in particular oxygen and nitrogen, at these interfaces, which has not been achieved before. The atomic-scale information on the arrangement of both light and heavy atoms is critical for realistic modeling of interface properties, such as interface strength and ion transport, and will facilitate increased control over the performance of ceramic and semiconductor materials for a wide-range of applications."

This is the first ever comprehensive treatment of NEXAFS spectroscopy. It is suitable for novice researchers as an introduction to the field, while experts will welcome the detailed description of state-of-the-art instrumentation and analysis techniques, along with the latest experimental and theoretical results.

This book details groundbreaking experiments for the sensing and imaging of terahertz-frequency electromagnetic radiation (THz) using Rydberg atoms. The major advances described include the development and implementation of a new technique for THz imaging using atomic fluorescence; the demonstration of a THz-driven phase transition in room-temperature atomic vapour; and a novel method for probing the excited-state dynamics of atoms using quantum beats. The work has formed the basis for several articles published in journals including Nature Photonics and the Physical Review, and has sparked industry interest, becoming the subject of ongoing collaborative research and development. This exceptionally well-written book provides a definitive account of terahertz sensing with Rydberg atoms.

This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.

This biography gives an insider view of 20th century German science in the making. The discovery by Max von Laue in 1912 of interference effects demonstrated the wave-like nature of X-rays and the atomic lattice structure of crystals. This major advance for research on solids earned him the Nobel Prize two years later, the ultimate acclaim as an exceptional theoretician. As an early supporter of Einstein's relativity theory, he published fundamental papers on light scattering as well as on matter waves and superconductivity. Laue may be counted among the few persons of influence in Germany who - as Einstein put it - managed to "stay morally upright" under Nazism. It is thus surprising that this is the first extensive biography of this famous scientist. Jost Lemmerich could hardly have been better equipped to describe German physics and physicists in the 1920s. His copiously illustrated historical account is based as much on scientific material as on private correspondence, creating a fascinating and convincingly detailed portrait.

This book describes a simple yet innovative method for performing Raman spectroscopy of samples submerged under liquid nitrogen. While Raman spectroscopy has proven to be a powerful tool for the characterization of the structure of matter in the gaseous, liquid, and solid phases, one major difficulty in its application has been laser damage to the material under investigation, especially for biological samples. This book demonstrates how immersion of the sample in liquid nitrogen protects the sample from thermal degradation and oxidation at high incident laser power and allows improvements in sensitivity and spectral resolution over room-temperature Raman spectroscopy, leading to the so-called RUN (Raman Spectroscopy Under liquid Nitrogen) technique. Cooling to liquid nitrogen temperature also allows the selection of the lowest energy molecular conformation for molecules which may have many low energy conformers. In addition, the presence of liquid nitrogen over a roughened surface improves the sensitivity of Surface Enhanced Raman Spectroscopy (SERS), enabling the closely related SERSUN (Surface-Enhanced Raman Spectroscopy Under liquid Nitrogen) technique. This book starts with the theoretical and experimental basics of Raman and polarized Raman spectroscopy, before moving on to detailed descriptions of RUN and SERSUN. Room temperature and RUN spectra are provided for over fifty molecules.

This book presents comprehensive studies of charge density waves (CDW) in a high-Tc cuprate superconductor using x-ray scattering techniques under uniaxial pressure. Specifically, the work addresses inelastic x-ray scattering studies under uniaxial pressure performed on the underdoped cuprate YBa2Cu3O6.67(p=0.12, Tc=65K) with incoming photon energy in the resonant (E=931.3 eV, Cu-L3 edge) and non-resonant conditions (E=17.794 keV). This is a completely new approach to the investigation of charge density waves. It revealed new features of charge density waves in cuprates, whose properties had previously been inaccessible..